Peripheral Neuron-Organoid Interaction Induces Colonic Epithelial Differentiation via Non-Synaptic Substance P Secretion
- Author:
Young Hyun CHE
1
;
In Young CHOI
;
Chan Eui SONG
;
Chulsoon PARK
;
Seung Kwon LIM
;
Jeong Hee KIM
;
Su Haeng SUNG
;
Jae Hoon PARK
;
Sun LEE
;
Yong Jun KIM
Author Information
- Publication Type:ORIGINAL ARTICLE
- From:International Journal of Stem Cells 2023;16(3):269-280
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background and Objectives:The colonic epithelial layer is a complex structure consisting of multiple cell types that regulate various aspects of colonic physiology, yet the mechanisms underlying epithelial cell differentiation during development remain unclear. Organoids have emerged as a promising model for investigating organogenesis, but achieving organ-like cell configurations within colonic organoids is challenging. Here, we investigated the biological significance of peripheral neurons in the formation of colonic organoids.
Methods:and Results: Colonic organoids were co-cultured with human embryonic stem cell (hESC)-derived peripheralneurons, resulting in the morphological maturation of columnar epithelial cells, as well as the presence of enterochromaffin cells. Substance P released from immature peripheral neurons played a critical role in the development of colonic epithelial cells. These findings highlight the vital role of inter-organ interactions in organoid development and provide insights into colonic epithelial cell differentiation mechanisms.
Conclusions:Our results suggest that the peripheral nervous system may have a significant role in the development ofcolonic epithelial cells, which could have important implications for future studies of organogenesis and disease modeling.
