Study on 17 pediatric patients aged from 1 to 12 years of old, 14 boys and 3 girls. Burn-caused agents were dry heat, wet-heat and chemicals. Patients were divided randomly into 2 groups: Group A (Study group) involved 10 patients with mean age of 5 years, average common burn area was 41% of body area and deep burn area was 35% of body area. Group B (control group) included 7 patients with mean age of 6 years. The average common burn area of these patients was 48% of body area and the deep burn area was 34%. In group A, the bacterial infection incidence was lower markedly than group B (p<0.05). P. aeruginosa caused sepsis had highest incidence (66.6%) in patients with positive culture. Skin implantation produced good and moderate results in group A. 10 patients received skin allograft in 16 operations. Mean time survival of skin grafts is 14.92 days.
burns, (Transplantation, Homologous)

Bone Transplantation ; Humans ; Mandible ; transplantation ; Transplantation, Homologous

After using the same homograft in 15 cases at our Institute, we only want to tell colleagues, because the number of the patients is still restricted and these cases are watched for only 2 years. A question is that we should only use the same spongy bones for mixing deformed face after accident or deformity by cleflip and palate, using the same hard bones should be only used instead of Mandibular.
Homologous Transplantation ; Surgery ; Oral Surgery

An orthotopic penetrating keratoplasty model was developed in the rat. An oversized (0.5 mm) graft was used and 8 interrupted sutures were applied. These sutures were not removed. Eleven grafts out of 13 were rejected by the 3rd week in the disparate group (Brown Norway rat to Lewis rat transplantation group), which was characterized by edema, opacity, and neovascularization. All grafts remained clear in the syngeneic group (Lewis rat to Lewis rat transplantation group). Immunohistochemical examination was performed. This model seems to be a reliable and reproducible one to evaluate rejection reaction in corneal transplantation.
Animals ; Female ; Graft Rejection ; Keratoplasty, Penetrating/immunology/*methods/pathology ; Lymphocyte Subsets/immunology/pathology ; Macrophages/immunology/pathology ; Rats ; Rats, Inbred BN ; Rats, Inbred Lew ; Transplantation, Homologous ; Transplantation, Isogeneic

Empyema after lung transplantation causes dysfunction of the allograft, and it has the potential to cause mortality and morbidity, but the technical difficulty of surgically treating this empyema makes this type of treatment unfavorable. We report here on two cases of decortication for empyema after lung transplantation.
Empyema ; Lung ; Lung Transplantation ; Transplantation, Homologous

Humans ; Intestine, Small ; transplantation ; Transplantation, Homologous ; methods

Comparison of pancreaticoduodenal transplants (PDT) and duct-ligated pancreas transplant (DLPT) were performed using syngeneic and allogeneic studies in rats. Both DLPT and PDT allogeneic grafts showed mild rejection. DLPT groups showed disorganized pathology and acini replaced by fat. Eventually, massive fibrosis was seen in the Islets of Langerhans, as well as rejection cellular infiltrates. In both PDT groups, normal histology was observed in the same period. Thus the effect of duct occlusion is highly detrimental for the grafts.
Animals ; Graft Rejection/pathology ; Ligation/adverse effects ; Pancreas/*pathology ; Pancreas Transplantation/*adverse effects ; Pancreatic Ducts/surgery ; Postoperative Period ; Rats ; Rats, Inbred Lew ; Rats, Sprague-Dawley ; Research Support, Non-U.S. Gov't ; Transplantation, Homologous ; Transplantation, Isogeneic

OBJECTIVETo screen specific functional proteins from lymphocyte involved in acute rejection using differential proteomics research.

METHODSTwo groups of rat liver transplantation models were established (isograft as control and allograft as acute rejection groups) by transplantation within Wistar rats, and between Wistar and SD. Morphology study were performed by histochemistry tech, followed by serum cytokine detection with ELISA. With 2-dimensional electrophoresis, proteomes of lymphocyte from the rats of different groups were separated and 2 proteome profiles were established. Comparing with the 2 profiles, 25 spots were selected and picked for in gel digestion, followed for analysis by matrix assisted laser desorption ionization (MALDI)-time of fly (TOF)/TOF MS. Two of the proteins were detected with Western blot to verify the changing profiles.

RESULTSThe results of morphology analysis and detection of cytokines (IL-2 and IFN-gamma) indicate that the animal models were established successfully and acute rejection happened after transplantation for 3 days. Twenty-five differential proteins were found out to be associated with acute rejection, among which 13 proteins were upregulated and 12 downregulated. The expression alterations of 2 proteins (beta-actin and carbonic anhydrase) are consistent with proteomics analysis results showing in Western blot.

CONCLUSIONSTwenty-five specific proteins exploiting mechanism of acute rejection are screened out, including IL-2 and carbonic anhydrase, which maybe benefit for the further works.

Acute Disease ; Animals ; Disease Models, Animal ; Graft Rejection ; metabolism ; pathology ; Interferon-gamma ; blood ; Interleukin-2 ; blood ; Liver ; pathology ; Liver Transplantation ; methods ; Lymphocytes ; metabolism ; Peptide Mapping ; Proteomics ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar ; Transplantation, Homologous ; Transplantation, Isogeneic

In this study antigen-independent factor in the pathogenesis of chronic rejection of organ transplants was examined. Kidney isografts and allografts were transplanted orthotopically into bilaterally nephroectomized rat recipients and studied functionally, morphologically and immunohistologically, at serial intervals up to 52 weeks after transplantation. Allograft recipients developed progressive proteinuria after 12 weeks, with gradual renal failure ultimately leading to death. At the same time, morphological changes, including progressive arteriosclerosis and glomerulosclerosis, tubular atrophy and interstitial fibrosis, developed. Immunohistologically, macrophages infiltrated glomeruli during this period and cytokines became unregulated. Our results showed that antigen-independent functional and morphological changes occurred in long-term kidney isografts and mimicked those appearing much earlier in allografts that reject chronically. Initial injury and extent of functioning renal mass is suggested to be important factor for such late changes.
Animals ; Graft Rejection ; etiology ; immunology ; pathology ; Graft Survival ; physiology ; Kidney ; immunology ; pathology ; Kidney Transplantation ; immunology ; methods ; pathology ; Proteinuria ; etiology ; Rats ; Rats, Inbred Strains ; Time Factors ; Transplantation, Homologous ; Transplantation, Isogeneic

By obsersation study, authors researched applying process of preperation of fresh homograft heart aortic valves. The result showed that: 85% (12/14) cases was good. The maximum kipped time was 8 weeks but the best time was in 6 weeks. The antibiotic-nutrient solution may be stored for up to 4 weeks in the dark at 4oC.
Heart Transplantation ; Heart-Lung Transplantation ; Aortic Valve ; Transplantation, Homologous