To determine the optimal condition of pollen germination. The pollen of Prunella vulgaris was cultured in vitro. Pollen germination rates were recorded using 10% H3BO4, 30% Ca(NO3)2, 20% MgSO4 and 10% KNO3 as the basic mineral medium with PEG of different molecular weight, sucrose of various density and multiple pH value. The rates were also measured under different cultivation temperature and pollen acquisition time. The optimal condition of pollen germination is 10% H3 BO4, 30% Ca(NO3)2, 20% MgSO4, 10% KNO3, and 25% PEG-4000 as the medium, with pH about 6. 5 and pollen acquired at the beginning of blossom.
Flowers ; physiology ; Pollen ; physiology ; Prunella ; physiology

To systemically evaluate the clinical efficacy and safety of oral preparation of Xiakucao with levothyroxine(LT4) on Hashimoto's thyroiditis(HT), so as to provide the evidence for its clinical application in the future. All the included studies were retrieved from four Chinese databases and three English databases from their inception to December 2019. ROB assessment tool of cochrane system and the evidence classification recommended by GRADE were used to evaluate the quality of evidences in all included studies. RevMan 5.3 was used for Meta-analysis of the outcomes. Software TSA 0.9(trail sequential analysis) was used to estimate the sample size for Meta-analysis. The results showed that 11 randomized controlled trials and totaling 1 215 patients were included. Preparation of Xiakucao combined with LT4 was adopted as intervention in experimental group, while patients in control group were treated with LT4 alone. Meta-analysis results showed that as compared with control group, the rate of total efficacy in experimental group was significant improved, including improvement of thyroid function and thyroid autoantibodies, shrinkage of thyroid gland and nodule, and improvement of clinical symptoms such as fatigue and cold intolerance(RR=1.15, 95%CI[1.09, 1.21]). The experimental group significantly decreased the serum level of thyroperoxidase antibody TPO-Ab(SMD=-0.91, 95%CI[-1.40,-0.41]), and reduced the size of left thyroid lobe(MD=-1.46, 95%CI[-1.82,-1.11]), right thyroid lobe(MD=-1.45, 95%CI[-1.96,-0.94]) and isthmus of thyroid gland(MD=-1.08, 95%CI[-1.20,-0.95]). After evaluation based on GRADEpro, the results showed that the evidence quality of all included studies was low or very low. The result of TSA showed that the cumulative sample size had reached the expected value. However, the pooled results may be affected by one study with high bias risk, with not so high effect intensity of evidences. From this review, we can see that in treatment of HT, intervention of preparation of Xiakucao combined with LT4 has advantages on improvement of clinical efficiency, decreasing serum level of TPO-Ab and shrinkage of thyroid gland. However, due to the quality of evidence, more rigorously designed and high-quality trials are needed in the future to verify the clinical efficacy and safety of preparation of Xiakucao in treating HT.
Hashimoto Disease ; Humans ; Iodide Peroxidase ; Prunella ; Thyroxine

OBJECTIVEThe contents of mineral elements in soil and each organ from cultivated and wild Prunella vulgaris were determined in order to provide a theoretical basis for exploitation and protection of wild resources and GAP study.

METHODThe contents of mineral elements (P, K, Ca, Mg, Na, Fe, Cu, Zn, Mn, Al) in soil and each organ from cultivated and wild P. vulgaris were determined by ICP-AES, and the results were analyzed by correlation analysis.

RESULTThe contents of mineral elements in soil and different organs of P. vulgaris were significant different between cultivated and wild species; the contents of P and Cu ranked in the order of spica > leaf > stem, the order of Mg, Ca, Zn, Fe, Mn, Al from high to low was leaf > spica > stem, the concentrations of K, Na were mainly distributed in leaf and stem, less in spica. A distinctly positive correlation between soil and spica for Fe content, and the relationship between soil and leaf, stem were significant positive correlation, the concentrations of Zn in spica, leaf, stem were positively related to soil, K contents in soil and stem were significant positive correlation. The different organs of cultivated and wild P. vulgaris had a strong tendency to accumulate P.

CONCLUSIONThere was no direct relationship between the concentrations of mineral elements in P. vulgaris, and their corresponding soil P. vulgaris adjusts the concentration of mineral elements mainly by active absorption.

Minerals ; analysis ; Prunella ; chemistry ; growth & development ; Soil ; analysis

OBJECTIVETo study the effect of water stress on physiological and the growth characters of Prunella vulgaris at the vegetative stage.

METHODThe plants were treated timely and quantitatively by weighting method, and the relevant index were measured under different water stress conditions.

RESULTWith the long time of water stress, the plants of SY2 and SY3 showed the quickest growth rate and the best of growth vigor. The protective enzyme and osmoregulation substance presented the trend of increase firstly and then decrease. Under the same time of water stress, with the increase of extent in treatment, the protective enzyme and osmoregulation substance presented the trend of increase firstly and then decrease, and SY2 and SY3 were the lowest.

CONCLUSIONThe protective enzyme and osmoregulation substance of P. vulgaris have a whole effect. The suitable water range of growth in P. vulgaris is 65%-80%.

Prunella ; growth & development ; physiology ; Stress, Physiological ; Water ; metabolism

Pancreatic lipase (PL) inhibitors were firstly screened from Prunella vulgaris with PL immobilized on carboxylic acid-terminated magnetic nanoparticles, then these possible inhibitors were identified by LC-MS/MS and mixed standards. Finally, their inhibitory effects and types on PL were tested by p-nitrophenol method. The results showed that four PL inhibitors were screened out from P. vulgaris and confirmed by LC-MS/MS and mixed standards. The IC₅₈ and inhibition types were as follows: caffeic acid [(252.3±3.6) mg·L⁻¹, anti-competitive inhibition], rutin [(91.2±1.6)mg·L⁻¹, competitive inhibition], hesperidin [(31.5±4.4) mg·L⁻¹, competitive inhibition] and ursolic acid [(41.3±2.2) mg·L⁻¹, competitive inhibition]. Their inhibitive types and abilities on PL were related to their molecular size, hydrophobicity and the number of hydrogen bond with PL triplet.
Chromatography, Liquid ; Lipase ; Plant Extracts ; Prunella ; Tandem Mass Spectrometry

OBJECTIVETo study the extraction flavonoids distribution in different parts of Prunella vulgaris from different populations.

METHODThe optimal extraction condition was selected by response surface method (RSM). The amounts of flavonoids were determined by colorimetric method.

RESULTThe optimum extraction condition was using 35% ethanol at 25 times of sawple volume, refluxing at 87 degrees C for 3.5 h. The amount of flavonoids was 2.16% -10.29% in P. vulgaris. The content of flavonoids was the highest in leaf while that in root was the lowest. And the content of flavonoids in spike after removed seeds was 27.6% higher than the one reserved seeds.

CONCLUSIONThe RSM was feasible for optimum extraction condition and the amount of flavonoids showed a signiticant regional distribution pattern in various populations of P. vulgaris.

Drugs, Chinese Herbal ; metabolism ; Feasibility Studies ; Flavonoids ; isolation & purification ; metabolism ; Plant Structures ; metabolism ; Prunella ; metabolism

OBJECTIVEThe accumulation of dry matter, nitrogen, phosphorus and potassium in Prunella vulgaris and their relationships under field condition were studied, in order to provide a basis for the rational fertilization.

METHODThrough the dynamic sampling of P. vulgaris in different growing stages, and the dry weight of plant, the amount of dry matter and the content of N, P and K within each organ of the plant were measured.

RESULTThe dry weight of P. vulgaris linearly increased with the increase of nitrogen, phosphorus and potassium accumulation, there existed a significant correlation at whole growth period. The maximum stage accumulation rate of dry matter from mid-April to the beginning of May, reached 63.71%. The amount of dry matter was mainly distribution in leaves from late March to mid-April. The distribution ratio in stem was the highest from beginning of May to late May. Until mid-June, the distribution ratio of dry matter in spicas was higher than that in other organs. The absorption amounts of N, P and K were lower before mid-April, and the nutrient element was concentrated in plant leaves. From late April to beginning of May, the N, P and K accumulation reached maximum of total absorptive capacity, which accounted for 72.35%, 59.82%, 87.41%, respectively, and the mineral elements were concentrated distribution of plant stems. When transferred to reproductive stage, both accumulation amount and distribution rate of nutrient elements in spicas increased rapidly, which reached the maximum until mid-June. From elongation stages to quaring period was the period of maximal accumulation of dry matter and showed the maximal efficiency of N, P and K nutrition.

CONCLUSIONThe time from elongation stages to quaring period is the most crucial period in the growth development stage of P. vulgaris, and should be paid more attention to the supply of mineral nutrition and moisture of plant to promote spicas formation and dry matter accumulation in this stage. The average N, P2O5 and K2O absorption amount per 100 kg dry matter of spicas were 1.62, 0.36, 2.88 kg respectively, and the ratio of N-P2O5,-K2O was 1 : 0.22: 1.77.

Absorption ; Nitrogen ; metabolism ; Phosphorus ; metabolism ; Potassium ; metabolism ; Prunella ; growth & development ; metabolism ; Seasons

OBJECTIVETo understand the effect of illumination, temperature on the germination of the Prunella vulgaris seeds.

METHODThe general germination method.

RESULTIllumination showed no clearly effect on the germination rate of the P. vulgaris seeds under proper temperature condition; However, illumination was the main factor to determine the germination rate of the P. vulgaris seeds when the temperature condition was unsuitable. The germination rate of the P. vulgaris seeds decreased with the time and increased when the marinating time was from 12 to 36 hours. When temperature reached 5-25 9 degrees C, the trend of the germination rate increased at frst and then decreased.

CONCLUSIONThe optimal germination condition of P. vulgaris seeds is 12 h of marinating time at the temperature of 20 degrees C under illumination.

Germination ; Lighting ; Plants, Medicinal ; growth & development ; Prunella ; growth & development ; Seeds ; growth & development ; Temperature

OBJECTIVETo establish HPLC fingerprint of Prunella vulgarise for quality control of the herbal medicine.

METHODA sunfire C18 analytical column was used. The mobile phase A was 1% acetic acid, and mobile phase B was methanol. The elution was in gradient mode and detection wavelength was set at 290 nm. The flow rate was 1.0 mL x min(-1) and the column temperature at 30 degrees C. The analysis time was 60 min.

RESULTThe similarity of 10 batches of P. vulgaris was not lower than 0.810. The fingerprints of the herbal medicine were classified P. vulgaris on the results of cluster analysis.

CONCLUSIONThis method is available for quality evaluation and control the quality of P. vulgaris.

Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; Prunella ; chemistry

Volatile oil components and the contents and types of amino acid in spica of Prunella vulgaris were analysed by GC-MS and amino acid analyzer. Esters, fatty acids, aromatic hydrocarbon, ketone and several alcohol compounds were identified by mass spectrum comparison. In these ingredients, beta-ionone smelled aroma of cedar, raspberry, nerolidol showed weak sweet soft orange blossom flavor, neroli tasted sweet and fresh, nerolidol tasted sweet with light aroma of wood, hexadecanal showed a weak aroma of flowers and wax, alpha-sinensal had rich and fresh sweet orange flavor. To some extent, these types of aromatic substances can affect the taste of herbal tea or decoction made of Spica Prunellae. Among amino acids detected, natural amino acids accounted for a larger proportion, and those natural amino acids showed bitterness, slight bitterness, sourness (freshness), sweetness, slight sweetness, sourness (slight freshness). The results indicated that bitter and slightly bitter amino acids have the greatest impacts on the sense of Spica Prunellae.
Amino Acids ; analysis ; Gas Chromatography-Mass Spectrometry ; Oils, Volatile ; analysis ; Prunella ; chemistry ; Taste