3 types the results were evaluated according to the efficacy of obtained vaccines have shown that the mininal infective dosages of poliovirus in production of oral live poliovirus vaccine in the renal cells of monkey which given the highest efficacy were 103 TCID 50 /bottle of Roux, 104TCID/bottle of Roux and 103 TCID/ bottle of Roux for type I (strain: WHO-IS-C), type II (strain: IIS-90A) and type III (IIIs-79A), respectively
Poliovirus ; Poliovirus Vaccine, Oral

In 2003, National Poliovirus Reference Laboratory-NIHE of Hanoi isolated three type 3 poliovirus strains from 466 stool specimens of 233 acute flaccid paralysis cases. All these poliovirus strains were confirmed as Sabin-like strains. 86 other non-polio enterovirus strains were isolated including 31 echoviruses, 4 coxsackie B viruses and 51 untyped enteroviruses. The results confirmed the sustainance of polio-myelitis free status in Viet nam.
Poliovirus ; Paralysis

Background: Paralysis has been eliminated in Vietnam in 2000. To maintain the achievement, Vietnam needs to have more than 90% of children under 1 age drinking 3 doses of OPV, controlling cases of acute flaccid paralysis and diagnosing for virology in laboratory to determine causes of the disease. Results of laboratory identifying Polio virus and enterovirus in patients with acute flaccid paralysis is evidence of successful in paralytic elimination annually in Vietnam.\r\n', u'Objectives: To of isolate polio virus and enterovirus in patients with acute flaccid paralysis suspected paralysis in 2005.\r\n', u'Subjects and methods: The study included 267 cases with acute flaccid paralysis from 29 provinces, cities in the North and six provinces in the Central of Vietnam during 2005.\r\n', u'Results:Results of surveillance for acute flaccid paralysis and indicators of samples tested in 2005 have reached the criteria regulated by the World Health Organization. 8 poliovirus strains and 56 other non-polio enterovirus strains were isolated from 538 stool specimens of above patients. All isolated poliovirus strains were confirmed as Sabin-like strains. 56 non-polio enterovirus strains included coxsaxkie A (3), conxsackieB (5), echoviruses (34), enterovirus 71 (1) and untyped enteroviruses (13). Wild polio virus was not isolated in 2005.\r\n', u'Conclusion: The result confirmed successfulness in maintaining of poliomyelitis free status in the North and 6 provinces of the Central of Vietnam in 2005.\r\n', u'\r\n', u'\r\n', u'
Paralysis ; Enterovirus ; Poliovirus

Background: \r\n', u'October 2000, Vietnam was acknowledged as the country to successfully eradicate the polio by WHO.This success was partly due to the oral polio vaccine (OPV) produced on the primary money cells by the Centre of Research, Production of vaccines and biologicals, Ha Noi. In 2006, the Centre developed the single polio vaccine type 1 from primary monkey cells.\r\n', u'Objectives: \r\n', u'To evaluate the safety and antibody titre .\r\n', u'Subjects and method: \r\n', u'6 lots of single polio vaccine type 1 (ISO- 90, Antibodies of Polio type 1,2,3; the standard sample F113 from Japanese research on Polio Institute\ufffd?\r\n', u'Using the tests (T- maker, D maker, PFU, CCID50) to check the safety of single polio vaccine type 1. \r\n', u'Results:\r\n', u'After 14 days, 6/6 lots of viruses were observed via the microscope that they stayed in well developed, and of no serious adverse affects.There was no appearance of degenerated cells. \r\n', u'Conclusion:\r\n', u'6/6 lots of single polio vaccine type 1 produced on Macca mulltta monkey kidney cells with the first time passage at POLYVAC in 2006 are safe and high antibody titre.\r\n', u'
Poliovirus Vaccine ; Oral ;

A study on the establishment of the effective standard for the national standard samples of vaccine of single vaccine type 1, 2 and 3 and triple vaccine (Sabin) produced in Vietnam was carried out. The sesults have shown that the National standard sample had a high unified and stable properties that meet the requirement of the world health organization (WHO). The National standard sample were used to evaluate the quality of sabin vaccine produced in Vietnam which showed that 450 batches (100%) sabin vaccine produced in the scientific center for the vaccine production during 1995-1999 obtained the standard of WHO.
Poliomyelitis ; Poliovirus Vaccine, Oral

After 5 years, since 1993, the strategies for polio eradication have been well implemented. Coverage of routine immunization with 3 doses of OPV to children under 1 year of age has always been maintained higher than 90%. Coverage of supplementary immunization with 2 doses of OPV to children under 5 years of age during the NIDs has been achieved greater than 98%, surveillance of all the acute flaccid paralysis cases with collection of 2 adequate stool specimens for the identification of wild poliovirus. The results of the surveillance show that wild poliovirus cases have disappeared, decreasing from 152 cases in 1993 to 0 cases in 1998. The last cases of wild poliovirus identified in Phu Yen province of Central region had the date of onset on the 29th January 1997.
Poliomyelitis ; Child ; Poliovirus

All children in suspicion of poliomyelitis from 28 provinces of North Vietnam (from 1993 to 1999) were enrolled in study performed with a form of inquiry concerning all related personal health documents and reports. Results shown that all children suffered from poliomyelitis with isolated wild poliovirus accounted for 73.4% were primarily non-immunized or under-dose-immunized. Conclusion recommended the continuation of oral immunization among all under-one year old children in combining with the providing two doses of oral vaccine per year for under five years old children
Poliomyelitis ; Poliovirus Vaccine, Oral

From 1991 to 1994, 203 poliovirus positive cases were identified among 559 patients with acute flaccid paralysis. Of these 207 isolates: 175 cases were caused by poliovirus type 1, 7 by type 2 and 11 type 3. Most of these isolates were intratypically differentiated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Of the studied 142 isolates: 107 were wild type 1 poliovirus strains, 7 were wild type 3 poliovirus strains, the remaining strains were Sabin vaccine-like strains. 107 wild type 1 poliovirus strains were divided into 7 groups by PCR-RFLP patterns and were also classified into 3 genomic groups by nucleotide sequencing and phylogenetic analysis. There were 3 unique wild poliovirus strains were circulating in Northern Vietnam during period 1991-1993. In 1994, no wild poliovirus was isolated
Poliovirus ; Viruses ; epidemiology

498 healthy children aged from 1 to 10 years were examined on the relationships between immunized status and a number of received OPV doses as well as the period of time after the last OPV dose. The results showed that the mixed antibody rate of 3 types was 86.5%; the monotype antibody rate was 97.8% in type 1, 99.7% in type 2 and 89.3% in type 3. Antibody titer was very high in all ages, GMTs of type 1 and type 2 were 143 and 146, respectively while that of type 3 was 31. Antibody rate and antibody titer with poliovirus had decreased year by year, have relationship with the number of received OPV doses, as well as the duration after the last OPV dose
Poliovirus ; Immunization ; child

In 1999, 49 vaccine samples had been collected from the field in routine immunization days. The potency of the samples had been verified in National Centre of Control of Medio-Biological Products, Ha Noi. The results showed that the potency of all the vaccine samples is at the acceptable limit, type 2 is the most stable and type 3 is the least stable one
Poliovirus Vaccine, Oral ; Drug Monitoring