2.Comparison of various estimation methods for the parameters of Michaelis-Menten equation based on in vitro elimination kinetic simulation data
Yong Soon CHO ; Hyeong Seok LIM
Translational and Clinical Pharmacology 2018;26(1):39-47
The Michaelis-Menten equation is one of the best-known models describing the enzyme kinetics of in vitro drug elimination experiments, and takes a form of equation relating reaction rate (V) to the substrate concentration ([S]) via the maximum reaction rate (Vmax) and the Michaelis constant (Km). The current study was conducted to compare the accuracy and precision of the parameter estimates in the Michaelis-Menten equation from various estimation methods using simulated data. One thousand replicates of simulated [S] over serial time data were generated using the results of a previous study, incorporating additive or combined error models as a source of random variables in the Monte-Carlo simulation using R. From each replicate of simulated data, Vmax and Km were estimated by five different methods, including traditional linearization methods and nonlinear ones without linearization using NONMEM. The relative accuracy and precision of the estimated parameters were compared by the median values and their 90% confidence intervals. Overall, Vmax and Km estimation by nonlinear methods (NM) provided the most accurate and precise results from the tested 5 estimation methods. The superiority of parameter estimation by NM was even more evident in the simulated data incorporating the combined error model. The current simulation study suggests that NMs using a program such as NONMEM provide more reliable and accurate parameter estimates of the Michaelis-Menten equation than traditional linearization methods in in vitro drug elimination kinetic experiments.
In Vitro Techniques
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Kinetics
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Methods
3.The kinetics of interaction between Fructose 1,6 - bisphosphat aldolase and derivatives of heparin
Journal of Vietnamese Medicine 2004;302(9):41-48
Fructose 1,6 bisphosphate adolase A and C (FPA A4 and C44) have been found to bind specifically to heparin in physiological ionic strength. The researcher found that activity of FPA was inhibited by heparin. The inhibition of FPA A4 and C4 depend on the degree of sulfation. Inhibition activity FPA of heparin increase directly proportional to molecule lenghth. Kinetic studies showed that: inhibitory mode of heparin on FPA was the linear mixed type inhibition, in which velocity can be driven to zero at high heparin concentration
Kinetics
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Fructose-Bisphosphate Aldolase
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Heparin
4.The Effect of Lifting Speed on Cumulative and Peak Biomechanical Loading for Symmetric Lifting Tasks.
Kasey O GREENLAND ; Andrew S MERRYWEATHER ; Donald S BLOSWICK
Safety and Health at Work 2013;4(2):105-110
BACKGROUND: To determine the influence of lifting speed and type on peak and cumulative back compressive force (BCF) and shoulder moment (SM) loads during symmetric lifting. Another aim of the study was to compare static and dynamic lifting models. METHODS: Ten male participants performed a floor-to-shoulder, floor-to-waist, and waist-to-shoulder lift at three different speeds [slow (0.34 m/s), medium (0.44 m/s), and fast (0.64 m/s)], and with two different loads [light (2.25 kg) and heavy (9 kg)]. Two-dimensional kinematics and kinetics were determined. A three-way repeated measures analysis of variance was used to calculate peak and cumulative loading of BCF and SM for light and heavy loads. RESULTS: Peak BCF was significantly different between slow and fast lifting speeds (p < 0.001), with a mean difference of 20% between fast and slow lifts. The cumulative loading of BCF and SM was significantly different between fast and slow lifting speeds (p < 0.001), with mean differences > or =80%. CONCLUSION: Based on peak values, BCF is highest for fast speeds, but the BCF cumulative loading is highest for slow speeds, with the largest difference between fast and slow lifts. This may imply that a slow lifting speed is at least as hazardous as a fast lifting speed. It is important to consider the duration of lift when determining risks for back and shoulder injuries due to lifting and that peak values alone are likely not sufficient.
Biomechanics
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Humans
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Kinetics
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Lifting*
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Male
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Shoulder
5.Kinetic Analysis of Na-K-ATPase Activity of Rabbit Cerebral Cortex by DDVP.
Byoung Jo JANG ; Young Woo LEE
Journal of Korean Neurosurgical Society 1982;11(1):17-22
The kinetics of inhibition of Na-K-ATPase activity by o, 0-dimethyl o-(2,2-dichlorovinyl) phosphate(DDVP) was investigated with homogenerate of rabbit cerebral cortex. The results were summarized as the follows : 1. Inhibition of Na-K-ATPase activity by DDVP showed a dose-dependent manner with an estimated I5.0 of 3.5x10(-4) M. 2. Altered pH and activity curves for Na-K-ATPase activity demonstrated comparable inhibition by DDVP in buffered acidic, neutral and alkaline pH renges. 3. Kinetic studies of cationic-substrate activation of Na-K-ATPase showed noncompetitive with respect to substrate and K+ and a mixed type inhibition with respect to Na+. These results suggest that DDVP appeared to exert its effects on Na-K-ATPase activity by interfering with the formation of Na-dependent phosphoenzyme.
Cerebral Cortex*
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Dichlorvos*
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Hydrogen-Ion Concentration
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Kinetics
6.Flowcytometric Analysis of DNA Content and Cell Kinetics in Nervous System Neoplasms.
Byung Kab HAN ; Tae Young KIM ; Jong Moon KIM
Journal of Korean Neurosurgical Society 1990;19(2):247-254
No abstract available.
DNA*
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Kinetics*
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Nervous System Neoplasms*
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Nervous System*
7.The effect of retinoic acid on cell kinetics in bromodeoxyuridine labelled hep G2 cell line.
Dae Ghon KIM ; Joong Ki AHN ; Dong Suck JANG ; Yee Yup KIM ; Se Ra LEE ; Soo Taek LEE ; Deuk Soo AHN
Korean Journal of Medicine 1993;45(5):561-571
No abstract available.
Bromodeoxyuridine*
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Hep G2 Cells*
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Kinetics*
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Tretinoin*
8.Flow model of internal-loop granular sludge bed nitrifying reactor.
Chinese Journal of Biotechnology 2003;19(6):754-757
Internal-loop granular sludge bed nitrifying reactor is a new type of aerobic nitrifying equipment and has shown a good potential for nitrification. To study the flow pattern and construct the flow model, the tracer tests were performed using pulse stimulus-response technique. Based on the experimental results, the flow pattern in the settling section and the circulating section of reactor were analyzed by axial dispersion model and tank-in-series model, respectively. The dispersion number D/uL of 0.00148 in the settling section indicates that its flow pattern is similar to plug flow reactor (PFR), and the series number N of 1.021 in the circulating section indicates that its flow pattern is similar to continuously stirred tank reactor (CSTR). During steady state, the theoretic hydraulic retention time is 360 min, and the actual hydraulic retention time is 341.2 min. The percentage of dead space in the reactor is 5.22%, thereinto the dead space caused by biomass (db ) is 0.75 % and the hydraulic dead space (dh) is 4.47%, which shows that the structural performance of the reactor is excellent. Based on the experiments and analysis, a model of CSTR and PFR in series was constructed. The actual hydraulic retention time distribution of the reactor is in good agreement with the model predictions. Since the relative error between them is 8.56%, the model is accurate to describe the flow pattern. The results have laid a foundation for the kinetic model of the reactor and will be helpful for its design and operation.
Bioreactors
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Kinetics
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Models, Theoretical
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Nitrites
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metabolism
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Sewage
9.Study on the kinetics of immobilized cells of Brevibacterium ammoniagenes MA-2 and Brevibacterium flavum MA-3.
Yong-Hong HU ; Shu-Bao SHEN ; Ping-Kai OUYANG
Chinese Journal of Biotechnology 2002;18(2):235-238
The kinetics of immobilized cells of Brevibacterium ammoniagenes MA-2 and Brevibacterium flavum MA-3 cells were studied. By means of both a theoretical analysis of diffusion in the gel particles and an experimental determination of apparent kinetic parameters, the intrinsic kinetic parameters of immobilized cells of B. ammoniagenes MA-2 and B. flavum MA-3 cells were obtained.
Brevibacterium
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isolation & purification
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metabolism
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physiology
;
Kinetics
10.Supralinearity of UV Irradiated Magnesium Aluminum Spinel.
Korean Journal of Medical Physics 2005;16(4):202-206
Three-dimensional thermoluminescence (TL) spectra from MgAl2O4 irradiated with UV light were measured over 300~600 K and 300~800 nm. The peak positions of TL glow curves were shifted to lower temperature with increasing the exposure time of UV light. The 476 K TL glow curve is due to the second kinetics and its activation energy and escape frequency factor are calculated to be 0.85 eV and 1.92x10(6) sec(-1), respectively. The TL spectra were split into 530 nm and 700 nm emission bands which were associated with V(2+) and Cr(2+), respectively. The linearity range of 700 nm emission band is smaller than that of 530 nm emission band, but the saturation time of 700 nm emission band is longer than that of 530 nm emission band.
Aluminum*
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Kinetics
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Magnesium*
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Ultraviolet Rays
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United Nations