1.Computational Approach for Biosynthetic Engineering of Post-PKS Tailoring Enzymes.
Genomics & Informatics 2008;6(4):227-230
Compounds of polyketide origin possess a wealth of pharmacological effects, including antibacterial, antifungal, antiparasitic, anticancer and immunosuppressive activities. Many of these compounds and their semisynthetic derivatives are used today in the clinic. Most of the gene clusters encoding commercially important drugs have also been cloned and sequenced and their biosynthetic mechanisms studied in great detail. The area of biosynthetic engineering of the enzymes involved in polyketide biosynthesis has recently advanced and been transferred into the industrial arena. In this work, we introduce a computational system to provide the user with a wealth of information that can be utilized for biosynthetic engineering of enzymes involved in post-PKS tailoring steps. Post-PKS tailoring steps are necessary to add functional groups essential for the biological activity and are therefore important in polyketide biosynthesis.
Clone Cells
;
Multigene Family
2.The Methods and Ethical Problems in the Human Clone.
Journal of the Korean Medical Association 1997;40(9):1146-1153
No abstract available.
Clone Cells*
;
Humans*
3.Situation of the Korean Medical Association about Animal Clone.
Journal of the Korean Medical Association 1999;42(9):826-829
No abstract available.
Animals*
;
Clone Cells*
4.The role of CDI on the antigen recognition of human CD3+CD4-CD8 T lymphocyte clone specific to M.tyberculosis.
Myung Sik CHOI ; Ju Young SEOH ; Dong Gyun LIM ; Ik Sang KIM ; Woo Hyun CHANG ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1993;28(6):505-519
No abstract available.
Clone Cells*
;
Humans*
;
Lymphocytes*
5.Construction and characterization of band-specific genomic clones by chromosome microdissection.
Gham HUR ; Eun Young KIM ; Sun Hwa PARK ; Yong Hyuck CHUN
Korean Journal of Anatomy 1993;26(4):435-449
No abstract available.
Clone Cells*
;
Microdissection*
6.Location and cloning of the polyhedrin gene of hyphantria cunea nuclear polyhedrosis virus.
Hyung Hoan LEE ; Mi Kyung LEE ; Il Hwan CHO ; Kwan Hee YOO
Journal of the Korean Society of Virology 1991;21(1):25-34
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
Nucleopolyhedrovirus*
7.Cloning and map location of thymidine kinase(TK) gene of Korean isolate bovine herpesvirus PQ strain.
Chang Hee KWEON ; Young Jin KEE ; Byung Joon KWON ; Soo Hwan AN
Journal of the Korean Society of Virology 1993;23(2):165-169
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
Thymidine*
8.Molecular Marker Related to Fruitbody Color of Flammulina velutipes.
Won Sik KONG ; Chang Hyun YOU ; Young Bok YOO ; Gyu Hyun KIM ; Kwang Ho KIM
Mycobiology 2004;32(1):6-10
White and brown strains of Flammulina velutipes were inter-crossed. All F1 showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.
Clone Cells
;
Flammulina*
;
Genes, vif
9.Cloning and protein expression of Actinobacillus actinomycetemcomitans cytolethal distending toxin subunit CdtA.
Sun Young KO ; Dong Keun JEONG ; So Hyun RYU ; Hyung Seop KIM
The Journal of the Korean Academy of Periodontology 2007;37(Suppl):339-351
No abstract available.
Actinobacillus*
;
Aggregatibacter actinomycetemcomitans*
;
Clone Cells*
;
Cloning, Organism*
10.Bioprospecting Potential of the Soil Metagenome: Novel Enzymes and Bioactivities.
Genomics & Informatics 2013;11(3):114-120
The microbial diversity in soil ecosystems is higher than in any other microbial ecosystem. The majority of soil microorganisms has not been characterized, because the dominant members have not been readily culturable on standard cultivation media; therefore, the soil ecosystem is a great reservoir for the discovery of novel microbial enzymes and bioactivities. The soil metagenome, the collective microbial genome, could be cloned and sequenced directly from soils to search for novel microbial resources. This review summarizes the microbial diversity in soils and the efforts to search for microbial resources from the soil metagenome, with more emphasis on the potential of bioprospecting metagenomics and recent discoveries.
Clone Cells
;
Ecosystem
;
Genome
;
Metagenome
;
Metagenomics
;
Soil
Result Analysis
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