1.Isolation and antimocrobial susceptibility of bacteroides fragilis.
Kag Hyun KIM ; Hyung Hoan LEE ; Hye Kyung CHUNG
Journal of the Korean Society for Microbiology 1992;27(2):115-123
No abstract available.
Bacteroides fragilis*
;
Bacteroides*
2.In vitro susceptibilities of bacteroides fragilis group organisms: comparison of the strains isolated in 1990 with 1991.
Kyung Won LEE ; Yoon Seob JUNG ; Oh Hun KWON ; In Ho CHANG ; Kap Joon YOON ; Sung Jin KIM
Korean Journal of Infectious Diseases 1993;25(1):27-32
No abstract available.
Bacteroides fragilis*
;
Bacteroides*
3.Genetic Structure in the Region Near the Sialidase Gens in Bacteroides fragilis.
Tomomi KUWAHARA ; Haruyuki NAKAYAMA ; Tomoya IWASA ; Suya OKAMOTO ; Yuuki TSUCHIHASHI ; Yoshinari OHNISHI
Journal of the Korean Society for Microbiology 2000;35(5):364-364
No Abstract Available.
Bacteroides fragilis*
;
Bacteroides*
;
Genetic Structures*
;
Neuraminidase*
4.Using FAB and PYG in coordinating with PCR on culturing, isolating and identifying enterotoxigenic bacteroides fragilis
Journal of Practical Medicine 2005;0(12):42-44
The result of the study of using FAB and PYG in isolation and identification of ETBF from fecal samples were as follows: FAB medium had more advantages as compared to PYG. Together with using FAB, the PCR method can differentiate and identity ETBF from the B. fragilis species. The detection limit of PCR was 103 CFU of bacteria per ml of fecal suspension. Among 27 B. fragilis trains, 66% of them were positive with primer pair GBF 201-210 by PCR. The findings showed that ETBF can be detected by culturing samples in FAB together with identification by PCR. More studies could be done for detecting ETBF from fecal samples, especially from diarrhea patients
Bacteroides fragilis
;
Polymerase Chain Reaction
5.Nuclear Factor - kappa B Activation and Signal Transduction Pathway in Human Intestinal Epithelial Cells Stimulated with Bacteroides fragilis Enterotoxin.
Jung Mogg KIM ; Soo Jin CHO ; Shin Jae KANG ; Hwoon Yong JUNG ; Yu Kyoung OH ; Young Jeon KIM ; Yang Ja CHO
Journal of Bacteriology and Virology 2001;31(4):343-351
No abstract available.
Bacteroides fragilis*
;
Bacteroides*
;
Enterotoxins*
;
Epithelial Cells*
;
Humans*
;
Signal Transduction*
6.Inflammatory Reaction in Intestinal Mucosa by Bacteroides fragilis Enterotoxin Stimulation: Regulation of Chemokine Gene Expression in Human Intestinal Epithelial Cells.
Jung Mogg KIM ; Soo Jin CHO ; Yu Kyoung OH ; Hee Bok OH ; Yang Ja CHO
Journal of Bacteriology and Virology 2001;31(2):113-121
No abstract available.
Bacteroides fragilis*
;
Bacteroides*
;
Enterotoxins*
;
Epithelial Cells*
;
Gene Expression*
;
Humans*
;
Intestinal Mucosa*
7.Basolaterial Secretion of CXC Chemokines by Human Intestinal Epithelial Cells in Response to Bacteroides fragilis Enterotoxin Via NF-kB Pathway.
Jung Mogg KIM ; Yu Kyoung OH ; Young Jeon KIM ; Hee Bok OH ; Yang Ja CHO
Journal of the Korean Society for Microbiology 2000;35(5):350-350
No Abstract Available.
Bacteroides fragilis*
;
Bacteroides*
;
Chemokines, CXC*
;
Enterotoxins*
;
Epithelial Cells*
;
Humans*
;
NF-kappa B*
8.Basolaterial Secretion of CXC Chemokines by Human Intestinal Epithelial Cells in Response to Bacteroides fragilis Enterotoxin Via NF-kB Pathway.
Jung Mogg KIM ; Yu Kyoung OH ; Young Jeon KIM ; Hee Bok OH ; Yang Ja CHO
Journal of the Korean Society for Microbiology 2000;35(5):350-350
No Abstract Available.
Bacteroides fragilis*
;
Bacteroides*
;
Chemokines, CXC*
;
Enterotoxins*
;
Epithelial Cells*
;
Humans*
;
NF-kappa B*
9.Comparison of the E-test with agar dilution susceptibility test by using bacteroides fragilis.
Hee Sun KIM ; Sung Kwang KIM ; Hwa Sun CHA
Yeungnam University Journal of Medicine 1993;10(1):135-143
The susceptibilities of 45 clinical isolates of bacteroidis fragilis to cefaclor, ciproflxacin and imipenem were determined by new method, E-test (AB Bidisk, Solna, Sweden) and were compared with those from conventional agar dilution method by using brain heart infusion, Mueller-Hinton and Wilk:..s Chalgren agar plates. And the susceptibility of 60 clinical isolates of bacteroides fragilis group (B. fragilis 45 strains, B. distasonis 6 strains, B. ovatus 5 strains, B. thetaiotaomicron 4 strains) to 5 quinolones (ciprofloxacin, enoxacin, norfloxacin, ofloxacin, pefloxacin) were determined by in vitro agar dilution method. Compared with agar dilution MICs for B. fragilis 45 strains, 90.3% of E-test MICs were within +/- 1 dilution of the agar dilutions, and 98.4% were within 2 dilutions. And there were little effect of different medium bases to determine MICs except Mueller-Hinton agar. On Mueller-Hinton agar, B. fragilis showed have or no growth activity. In vitro susceptibility of B. fragilis group to quinolones, most of the test strains showed resistant patterns to quinolones except ofloxacin and there was little difference of susceptibility patterns between species of B. fragilis group.
Agar*
;
Bacteroides fragilis*
;
Bacteroides*
;
Brain
;
Cefaclor
;
Enoxacin
;
Heart
;
Imipenem
;
Norfloxacin
;
Ofloxacin
;
Quinolones
10.Characterization of a Potential Pathogenicity Island of Enterotoxigenic Bacteroides fragilis (ETBF) Strains.
Journal of the Korean Society for Microbiology 1998;33(3):273-280
Enterotoxigenic B. pagilis (ETBF) strains which produce a 20 kDa zinc metalloprotease toxin (BFI) have been associated with diarrheal disease of animals and young children. Using B. pngilis toxin gene (bfi) from strain 86-5443-2-2 (piglet isolate) as a probe, the gene was identified in 74/77 human and animal ETBF strains but only 2/97 non-toxigenic B. fragilis (NTBF) strains. The region flanking bp was mapped with several restriction enzymes and 8 resriction fragments aacent to bft were used to probe colony blots of 77 KTBF and 97 NTBF strains. All 74 bft-positive ETBF strains hybridized to the 8 probes spanning a ca. 18 kb chromosomal region; however, this 18 kb region was absent in the 3 ETBF strains lacking p, and 47 of the 97 (48%) NTBF strains lacked the entire 18 kb region. Of note, the 2 NTBF strains containing btf did not have a ca. 12 kb region upstream of btfp. A ca. 9 kb fragment flanking the btf gene has been sequenced. Analysis of this data revealed several open reading frames (ORF) of which 3 are of particular interest (ORFs 1, 2 and 3). ORF1 and ORF3 encode proteins with significant homology to mobilization proteins, and ORF2 encodes a protein with significant homology to metalloprotease proteins, but only 50% similarity and 30% identity to BFf. These results suggest: 1) the btf genes are flanked by at least 18 kb of DNA largely unique to ETBF strains indicating a putative pathogenic island, 2) another metalloprotease protein present in ETBF strains may contribute to the pathogenicity and variable virulence of these diarrheagenic strains and 3) the pathogenic island may be mobiTized among different Bacteroides strains, and possibly among different species of intestinal bacteria.
Animals
;
Bacteria
;
Bacteroides fragilis*
;
Bacteroides*
;
Child
;
DNA
;
Genomic Islands*
;
Humans
;
Open Reading Frames
;
Virulence*
;
Zinc
Result Analysis
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