The short time antibacterial effect of tetracaine hydrochloride was studied. S. aureus, Coagulase Negative Staphylococcus and P. aeruginosa were each incubated with tetracaine hydrochloride(preservative free) for 18 hours or for 2 minutes and then diluted and cultured on nutrient agar plate. Colony counts were done after 18 hours. In cases of 18 hours incubation, there was no growth of microbials in 0.5%, 0.1% tetracaine hydrochloride, but there was no inhibitory effect of 0.02% of tetracaine hydrochloride on growth of microbials, irrespective of inoculum amount. In cases of 2 minutes incubation with 0.5% tetracaine hydrochloride, there was no difference between the amount of microbial inoculum and colony count. Above in vitro study indicates that tetracaine hydrochloride has no inhibitory effect on bacterial growth in short time exposure less than 2 minutes.
Agar ; Coagulase ; Staphylococcus ; Tetracaine*

The short time antibacterial effect of tetracaine hydrochloride was studied. S. aureus, Coagulase Negative Staphylococcus and P. aeruginosa were each incubated with tetracaine hydrochloride(preservative free) for 18 hours or for 2 minutes and then diluted and cultured on nutrient agar plate. Colony counts were done after 18 hours. In cases of 18 hours incubation, there was no growth of microbials in 0.5%, 0.1% tetracaine hydrochloride, but there was no inhibitory effect of 0.02% of tetracaine hydrochloride on growth of microbials, irrespective of inoculum amount. In cases of 2 minutes incubation with 0.5% tetracaine hydrochloride, there was no difference between the amount of microbial inoculum and colony count. Above in vitro study indicates that tetracaine hydrochloride has no inhibitory effect on bacterial growth in short time exposure less than 2 minutes.
Agar ; Coagulase ; Staphylococcus ; Tetracaine*

KOH examination is a simple, rapid and diagnostic procedure to confirm dermatophytic infections. It is important to select a proper examination site of the lesion. To determinate the proper examination site of the lesion, mycologic studies were done with multiple specimens collected from the center, margin and out of margin of the ring-shaped dermatophytic skin lesion on the 58 patients. The results were as follows. Positive rate of KOH wet smear was 94.8% at the center and 100% at the margin of the lesions, 22.4% at the 1 cm and 5.2% at the 2 cm out of the lesions. The more hyphae were found in the lesion, the more hyphae were found out of the lesion. Culture was done on the Sabouraud's glucose agar from the highest KOH positive area and the positive culture was 48 strains (82.8%) of 58 patients. These findings suggested that the ring-shaped active margin was the best site to examine mycologic studies.
Agar ; Glucose ; Humans ; Hyphae ; Skin*

BACKGROUND: Early and appropriate antibiotic treatment improves the clinical outcome of patients with septicemia; therefore, reducing the turn-around time for identification (ID) and antimicrobial susceptibility test (AST) results is essential. We established a method for rapid ID and AST using short-term incubation of positive blood culture broth samples on solid media, and evaluated its performance relative to that of the conventional method using two rapid ID systems and a rapid AST method. METHODS: A total of 254 mono-microbial samples were included. Positive blood culture samples were incubated on blood agar plates for six hours and identified by the MicroFlex LT (Bruker Daltonics) and Vitek-MS (bioMeriéux) systems, followed by AST using the Vitek2 System (bioMeriéux). RESULTS: The correct species-level ID rates were 82.3% (209/254) and 78.3% (199/254) for the MicroFlex LT and Vitek-MS platforms, respectively. For the 1,174 microorganism/antimicrobial agent combinations tested, the rapid AST method showed total concordance of 97.8% (1,148/1,174) with the conventional method, with a very major error rate of 0.5%, major error rate of 0.7%, and minor error rate of 1.0%. CONCLUSIONS: Routine implementation of this short-term incubation method could provide ID results on the day of blood culture-positivity detection and one day earlier than the conventional AST method. This simple method will be very useful for rapid ID and AST of bacteria from positive blood culture bottles in routine clinical practice.
Agar ; Bacteria* ; Humans ; Methods ; Sepsis

BACKGROUND: The aim of this study is to evaluate the effectiveness of automated ozonated water endoscopic reprocessing system (AORS). MATERIALS AND METHODS: Thirty cases were collected and randomly assigned to 3 groups according to the disinfection methods (Group A, AORS for 5 minutes; Group B, AORS for 10 minutes; Group C, automated disinfection with superoxidized water for 3 minutes 30 seconds). After disinfection was finished, samples were collected from the tip of scopes (Site 1, S1) and rinsing water through biopsy channel (Site 2, S2). Samples were inoculated in blood agar plate for 48 hrs, and then colony count was evaluated. RESULTS: Culture positive rate of S1 was 0% in all three groups. Culture positive rates of S2 were 70% (7/10), 70% (7/10) and 90% (9/10) in group A, group B and group C, respectively. High culture rate group (> or = 1 CFU/ml rinsing water) was 0% (0/10), 30% (3/10) and 70% (7/10) in group A, group B and group C, respectively. Disinfection efficacy between group A and C showed a significant difference in high culture rate (P<0.05). CONCLUSIONS: AORS for 5min was at least equally effective in endoscopic reprocessing compared with the conventional superoxidized water system.
Agar ; Biopsy ; Disinfection* ; Endoscopes ; Water*

BACKGROUND: The aim of this study is to evaluate the effectiveness of automated ozonated water endoscopic reprocessing system (AORS). MATERIALS AND METHODS: Thirty cases were collected and randomly assigned to 3 groups according to the disinfection methods (Group A, AORS for 5 minutes; Group B, AORS for 10 minutes; Group C, automated disinfection with superoxidized water for 3 minutes 30 seconds). After disinfection was finished, samples were collected from the tip of scopes (Site 1, S1) and rinsing water through biopsy channel (Site 2, S2). Samples were inoculated in blood agar plate for 48 hrs, and then colony count was evaluated. RESULTS: Culture positive rate of S1 was 0% in all three groups. Culture positive rates of S2 were 70% (7/10), 70% (7/10) and 90% (9/10) in group A, group B and group C, respectively. High culture rate group (> or = 1 CFU/ml rinsing water) was 0% (0/10), 30% (3/10) and 70% (7/10) in group A, group B and group C, respectively. Disinfection efficacy between group A and C showed a significant difference in high culture rate (P<0.05). CONCLUSIONS: AORS for 5min was at least equally effective in endoscopic reprocessing compared with the conventional superoxidized water system.
Agar ; Biopsy ; Disinfection* ; Endoscopes ; Water*

The purpose of this study was to investigate the cytotoxicity and mutagenicity of denture ase resins. According to manufacturer's instructions, resin specimens were made. Group 1: heat-polymerizing acrylic resin (Luciton 199(R)). Group 2: heat-polymerizing acrylic resin containing polyhedraloligosilsesquioxane(POSS esin). Group 3: auto-polymerizing acrylic resin (Repair Acrylic(R)). Group 4: direct relining auto-polymerizing acrylic resin (Tokuso Rebase(R)). Fresh specimens, 24 hrs. and 72 hrs. soaked specimens in distilled water were made. Responses with metabolic assay and mutagenesis assay to eluates from resin specimens were measured. Cultures with medium alone provided controls. Cytotoxicity was assessed with agar overlay test. The results were as follows: 1. Group 4 showed higher cytotoxicity than Group 1, Group 2 and Group 3 in fresh, 24-and 72-hour immersion cases (p<.05). Group 3 showed higher cytotoxicity than Group 2 in fresh cases and showed higher cytotoxicity than Group 1 and Group 2 in 24-and 72-hour immersion cases (p<.05). Group 1 and Group 2 showed no significant difference. 2. All acrylic denture base resins showed significant increase of cell activity as immersion time increased (p<.05). 3. Auto-polymerizing acrylic denture base resins showed higher cytotoxicity than heat-polymerizing acrylic denture base resins (p<.05). 4. All acrylic denture base resins showed lower mutagenicity than controls (p<.05).
Agar ; Denture Bases* ; Dentures* ; Immersion ; Mutagenesis ; Water

Herein, we report a case in which cholestasis caused discrepancy in high-density lipoprotein (HDL)-cholesterol levels measured using various methods. The discrepancy in HDL-cholesterol level originated from the abnormal increase in the level of an unusual lipoprotein, apo E-rich HDL, in the patient's serum. An abnormal slow alpha-migrating lipoprotein was observed on agarose gel electrophoresis, and an abnormal large-sized HDL was observed in a lipoprotein subfraction study. The level of apolipoprotein E was elevated.
Apolipoproteins ; Cholestasis ; Electrophoresis, Agar Gel ; Lipoproteins

Herein, we report a case in which cholestasis caused discrepancy in high-density lipoprotein (HDL)-cholesterol levels measured using various methods. The discrepancy in HDL-cholesterol level originated from the abnormal increase in the level of an unusual lipoprotein, apo E-rich HDL, in the patient's serum. An abnormal slow alpha-migrating lipoprotein was observed on agarose gel electrophoresis, and an abnormal large-sized HDL was observed in a lipoprotein subfraction study. The level of apolipoprotein E was elevated.
Apolipoproteins ; Cholestasis ; Electrophoresis, Agar Gel ; Lipoproteins

OBJECTIVES: The purpose of this study was to provide photodynamic bactericidal effect against Enterococcus faecalis by erythrosine concentrations and LED irradiation times. METHODS: Erythrosine was used as a photosensitizer and green LED (3 Watt, 520-530 nm) was used as light source. E. faecalis ATCC 1943 and E. faecalis ATCC 29212 were used in this study. Approximately 10(5) CFU of bacteria were added in wells of a 96-well microtitration plate. For examining the effects of concentrations of erythrosine, 0, 0.625, 1.25, 2.5, 5, and 10 microM of erythrosine were added in wells containing bacteria. The irradiation time with LED was 30 sec. In another set of experiment, the effect of irradiation time for killing of bacteria was investigated by increasing irradiation time from 0 to 30 s with 10 microM of erythrosine final concentration. After irradiation, each sample was serially diluted with PBS and 50 microl of diluents was spread on duplicate blood agar plates. The plates were incubated for 72 h at 37degrees C under aerobic conditions and the number of CFU was determined. The experiments were repeated four times. The results were analyzed using one-way ANOVA, and Tukey's multiple comparison at a significance level of 0.05. RESULTS: When the erythrosine concentrations were more than 2.5 microM, E. faecalis ATCC 29212 was significantly decreased (P<0.05). The more erythrosine concentrations increased, the more E. faecalis ATCC 1943 decreased statistically significantly (P<0.05). In another set of experiment, when LED irradiation time was more than 20 s, E. faecalis ATCC 1943 decreased significantly (P<0.05), and if the irradiation times was more than 5 s, E. faecalis ATCC 29212 decreased significantly (P<0.05). CONCLUSIONS: PDT using erythrosine and green LED was found to be an effective method in killing E. faecalis.
Agar ; Bacteria ; Enterococcus faecalis* ; Erythrosine* ; Homicide ; Photochemotherapy