This article was aimed to study the physiological mechanisms in frozen storage to reduce early bolting of Angelica sinensis. By analyzing the root soluble sugar, soluble protein, MDA content changes before and after -10oC processing of the Angelica sinensis seedlings of different size from different sources , the physiological mechanisms in frozen storage reducing early bolting of A . sinensis had been studied. The results indicated that the greater seedlings , the higher content of soluble sugar , lower protein content and higher MDA . There were no obvious differences on the soluble sugar , soluble protein and MDA content of the same size seedlings from different sources . After -10oC freezing , the content of soluble sugar and protein in the seedlings with the same size were decreased. Meanwhile, the content of MDA was increased. It was concluded that the content of soluble sugar, soluble protein and MDA were obviously related to the size of Angelica sinensis seedlings from the same source before and after low temperature process .

Study on traditional Chinese medicine (TCM) syndrome has been lasted for more than 50 years, which made the syndrome problems become syndrome difficulties. TCM syndrome has become a bottleneck problem and the key scientific problem which restricted the development of TCM theories. The exploration of syndrome difficultiesshowed that syndrome is a unique complex with double structure, which contained the functional structure andsubstantial structure. Uncovering the mathematical sciences connotation inherit in TCM theoretical system, exploring methods of objective and quantitative characterization of the syndrome, deciphering the functional structure of syn-drome, can make a key technical route for TCM theoretical system knowledge innovation in order to reveal the sub-stantial structureof syndrome. It is hopeful to re-open a new era of TCM theoretical innovation and great practice.

This study was aimed to optimize the alcohol precipitation techniques for honeysuckle extract in order to standardize the production of honeysuckle extract of Qingkailing Injection and reduce the differences among batches. The orthogonal test was applied in this study. The content of chlorogenic acid and galuteolin were taken as compre-hensive indicators. Multi-index comprehensive scoring method was used in the data analysis. Three influencing fac-tors, which were the fluid temperature, the stirring speed and the speed of adding alcohol, were optimized in the al-cohol precipitation techniques. The results showed that the optimal alcohol precipitation techniques were when the fluid temperature was 20℃, the stirring speed was 240 rpm and the speed of adding alcohol was one time of the ma-terial per minute. It was concluded that the optimized alcohol precipitation process was stable and feasible.

This study was aimed to establish an Ultra Fast Liquid Chromatography-Photo Diode Array (UFLC-PDA) method for the simultaneous determination of five chemical components, which included chlorogenic acid, loganin, sweroside, evodia rutaecarpa glycosides and triplostoside A, in Pterocephalus hookeri h eck. Agilent Poroshell 120 SB-C18 (100 mm í 4.6 mm, 2.7 μm) was adopted, with acetonitrile-0.2% phosphoric acid solution in gradient elution as the mobile phase at the flow rate of 1.0 mL·min-1. And the injection volume was 0.4 μL. The detection wavelength was set up at 237 nm and 325 nm. And the column temperature was 30℃. The results showed that the calibration curve was linear within the range of 8.72~218.0, 1.52~38.0, 2.44~61.0, 29.36~734.0, 3.00~75.0μg·mL-1 (r > 0.999 6, n=9) for chlorogenic acid, loganin, sweroside, evodia rutaecarpa glycosides and triplostoside A, respectively. The average recovery rates were 99.46%, 99.41%, 100.14%, 98.89%, and 99.42%, respectively. The RSD was 0.69%, 0.66%, 0.60%, 1.21%, and 0.64%, respectively (n = 9). It was concluded that this method was simple, accurate and reproducible, which can be used for the simultaneous determination of the content of five chemical components in P. hookeri.

As an ancient medicinal and editable resource, pomegranate has a long usage history in the Tibetan medicine, Uyghur medicine and Indian Ayurvedic medicine. Pomegranate pericarp, seeds and flowers are widely used in both TCM and other ethnic medicine. Therefore, it is important to use modern analytical and detective methods to identify the chemical components in order to clarify its material basis and improve the quality control standards. This article summarized the main chemical compositions and quality evaluation methods of pomegranate pericarp, seeds and juice. Analysis was given on questions occurred during the study process. This study provided scientific data for pharmacodynamic material basis of different parts of pomegranate. It also provided scientific references for compre-hensive development and utilization of pomegranate.

Donguibogam was introduced from traditional Chinese medicine (TCM) instead of the local representa-tive work of the Democratic People's Republic of Korea (DPRK). It is also the concrete manifestation of theories of different schools of doctors according to Dr. Xu Jun's medical thoughts. In this paper, from Dr. Xu's advocacy to Taoism and the concept of aftercare spirit, the exploration was made on health cultivation for different body constitu-tions. According to the health cultivation effects of the medicinals and edible food, the focus was made on the sys-tematic collation and induction of more than 1 400 kinds of herbs contained in the soup liquid from papers in three volumes. The medicinals were divided into eight categories, which were benefiting qi, nourishing yin, tonifying yang, clearing heat and promoting dieresis, drying dampness and resolving phlegm, promoting blood circulation for remov-ing blood stasis, dispersing stagnated liver qi, nourishing blood for hemostasis. Donguibogam specified the health cultivation concept from the correspondence between the body constitution and the medicinal performance. It has a certain guiding significance to the current health cultivation.

This study was aimed to observe changes of key molecular in insulin signaling pathway in the hypothala-mus of rats to explore the mechanism of spleen yin deficiency diabetes-associated cognitive decline (DACD) and Zibu Piyin Recipe (ZBPYR) in order to provide new ideas and new clues for treatment of DACD. Rats were randomly divided into five groups, which were the control (Cont) group, diabetes (DM) group, spleen yin deficiency (pi) group, spleen yin deficiency diabetes (piDM) group and the ZBPYR group. Insulin receptor substrate 1 (IRS-1) serine phos-phorylation levels and protein kinase B (PKB/Akt) serine phosphorylation levels which were involved in the insulin signaling were observed by western blotting in the hypothalamus to determine whether there were insulin signaling obstacles in the hypothalamus of rats. The results showed that the expression of p-IRS-1ser in the DM group, pi group and piDM group was increased compared with the Cont group (P< 0.05); while the p-Akt expression of the DM group and piDM group was decreased (P< 0.05). The expression of p-IRS-1ser in the ZBPYR group decreased compared with the DM group and piDM group (P< 0.05); while the level of p-Akt increased compared with the DM group and piDM group (P < 0.05). It was concluded that insulin signaling was not transduced normally in the hy-pothalamus of the DM group, pi group and piDM group. Insulin resistance may occur in the hypothalamus. And ZBPYR can correct insulin signaling transduction disorder.

This study was aimed to measure the classification and content of inorganic elements in different parts of flowers, leaves and stems of cultivated Trollius chinensis. Meanwhile, a comparison was made in the classification and content of wild authentic T. chinensis and those of cultivated T. chinensis. At last, the medicinal quality of cul-tured T. chinensis was evaluated. An experimental method of flame atomic absorption spectrophotometry was adopted to analyze the classification and content of inorganic elements in different parts of flowers, leaves and stems of culti-vated T. chinensis and those of wild authentic T. chinensis. The results showed that the classification of inorganic el-ements in cultivated T. chinensis and those in wild authentic T. chinensis were the same. The contents of K, Ca, Mg, Fe, Zn, Mn and Cu in both cultivated T. chinensis and wild authentic T. chinensis were basically the same. Most of the classification of inorganic elements in leaves of cultivated T. chinensis was the same with those in flowers. The content of inorganic elements was rich. It was concluded that the classification of inorganic elements in cultivated T. chinensis was the same with those in wild authentic T. chinensis. The main content was also similar. The planting of T. chinensis can meet the medicinal standards. Meanwhile, the leaves of T. chinensis can also be developed for medicinal value.

This study was aimed to observe the effect of Xiao-Chai-Hu Decoction (XCHD) with different doses of Bupleurum on body temperature and content of IL-1β, IL-6 and TNF-α in serum among fever rat models induced by lipopolysaccharide (LPS) in order to and investigate its action mechanism. A total of 50 rats were randomly divid-ed into 5 groups, including the normal control group, fever model group, XCHD group (high-dose), XCHD group (middle-dose), and XCHD group (low-dose). The fever rat model was established by intraperitoneal injection of LPS (20 μg/kg). At the same time, all rats were given corresponding XCHD or saline solution by gavage. The body tem-perature of rats (Ti) was measured every 30 min for 240 min after the injection of LPS. Change values of body tem-perature △T(△T=Ti-T0) were calculated. After the last measurement of body temperature, blood was taken for the de-tection of IL-1β, IL-6 and TNF-α level in serum with ELISA. The results showed that the body temperature of XCHD group (high-dose) was obviously decreased at the time point of 3 h, 3.5 h, 4 h (P< 0.01, or P< 0.05). This temperature reducing effect at the time point of 3.5 h and 4 h was better than the XCHD group (middle-dose) and XCHD group (low-dose, P< 0.01, or P< 0.05). Compared with the fever model group, all three XCHD groups can obviously reduce the IL-1β level (P< 0.05). Among them, the XCHD group (high-dose) had obvious effect (P<0.01). Compared with the fever model group, the XCHD group (high-dose) can obviously reduce contents of IL-6 and TNF-α in serum (P< 0.05). It was concluded that XCHD can reduce fever induced by LPS in rats and decrease contents of IL-1β, IL-6 and TNF-α in serum. This effect has a positive dose-effect relationship with the dose of Bu-pleurum in a certain range.

This study was aimed to establish an HPLC method to simultaneous determine 6 kinds of monoester and diester aconitum alkaloids. The content of alkaloids in aconite roots, black and white prepared lateral root of aconite and the compatibility of aconite roots with rhubarb were determined. This study provided reference for the interpreta-tion of the attenuation of processing and compatibility of medicines from chemical component aspect. The HPLC analysis was performed on a Phenomenex Gemini C18 (4.6 mm í 250 mm, 5 μm) with 40 mmol·L-1 ammonium ac-etate (adjusted to pH 9.8 with ammonia water) and acetonitrile as mobile phase, with a gradient elution at the flow rate of 1.0 mL·min-1 and a detection wavelength of 235 nm. The results showed that 6 alkaloids in aconite roots achieved favorable separation and a good linearity relationship (r > 0.999) over the studied concentration range. The extraction recoveries were ranged from 96.9% to 102.4% for the 6 alkaloids. The content of diester alkaloids de-creased markedly in processed products and the compatibility of aconite roots with rhubarb compared with the raw a-conite roots. It was concluded that this method was stable, reliable, simple and practical. It can be used for the si-multaneous determination of 6 kinds of monoester and diester aconitum alkaloids in aconite roots. This processing and compatibility can significantly reduce the content of alkaloids in aconite roots in order to reduce its toxicity.