OBJECTIVEThe purpose of this study was to investigate the transcription and expression levels of human leukocyte antigen-DR at different stages of oral squamous cell carcinomas.

METHODSThe specific monoclonal antibody and beta-locus specific oligonucleotide probes of human leukocyte antigen-DR were employed in this study. A total of 32 primary oral squamous cell carcinomas, 15 metastatic focuses and 26 histologically normal oral epithelia, were detected for the presence of the human leukocyte antigen-DR molecule by using immunohistochemistry and in situ hybridization.

RESULTSThe human leukocyte antigen-DR expression of the primary focuses was significantly higher than that of the normal epithelia (P < 0.05), but their expression did not show statistically difference between the metastatic focuses and the normal epithelia. The immunohistochemistric results were identical with those of in situ hybridization.

CONCLUSIONThe abnormal higher expression of the HLA-DR is a common character of primary oral squamous cell carcinomas, but it may be not relevant to the metastasis of tumors.

Carcinoma, Squamous Cell ; genetics ; immunology ; HLA-DR Antigens ; biosynthesis ; genetics ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Mouth Mucosa ; immunology ; Mouth Neoplasms ; genetics ; immunology

OBJECTIVEThe aim of this study was to investigate the mRNA expression levels of human leukocyte antigen Class I at different progressive stages of human oral squamous cell carcinomas.

METHODSThe expression of mRNA of human leukocyte antigen--B/C was detected in 23 primary tumors, 10 metastatic focuses and 11 histological normal oral epithelia using in situ hybridization method with a digoxigenin--labeled DNA probe. The probe was human leukocyte antigen--B/C locus specific.

RESULTSThe hybridization signals were present in the cytoplasm of either normal epithelia or tumor cells. The integrated optical density values of the hybridization signals were detected with the aid of an image analysis system. The results showed that the average integrated optical density values of the primary tumors were statistically lower than the normal oral epithelia (P < 0.05), but there was no significant difference between metastatic tumors and the primary tumors or the normal epithelia. The integrated optical density values measured in the metastatic tumors also did not show statistically differences compared with the primary tumors of the same patients.

CONCLUSIONImpaired regulation of human leukocyte antigen--B/C transcription could occur but might not be directly associated with metastasis of oral squamous cell carcinomas.

Carcinoma, Squamous Cell ; genetics ; immunology ; HLA-B Antigens ; biosynthesis ; genetics ; HLA-C Antigens ; biosynthesis ; genetics ; Histocompatibility Antigens Class I ; biosynthesis ; genetics ; Humans ; In Situ Hybridization ; Mouth Neoplasms ; genetics ; immunology ; RNA, Messenger ; biosynthesis ; genetics ; Transcription, Genetic

OBJECTIVEThe aim of this study was to explore the expression of basic fibroblast growth factor (bFGF) and fibronectin during the healing process of allograft tissue engineering skin.

METHODSThe tissue engineering skin that was obtained from neonatal SD rats was cultured in the lab. Afterwards, the skin was grafted into adult SD rats, and the expressions of bFGF and fibronectin were detected on the 7th, 10th, 14th, 20th and 30th day after the allograft of the tissue engineering skin. The autografted skin in 15 adult Wistar rats and the normal skin in 15 Wistar were treated as the control. HE staining and immunohistochemical staining were used to examine the healing of grafted skin.

RESULTSThe expression of bFGF and fibronectin was the strongest on the 10th day after graft, and was weaker before the 10th day and after the 14th day. The expression changes of bFGF and fibronectin were similar as they were in the autograft group.

CONCLUSIONThe expression changes of bFGF and fibronectin in the tissue engineering skin during the process of wound healing were similar to those of autografted skin, and these can promote the repair of tissue engineering skin allograft.

Animals ; Animals, Newborn ; Cells, Cultured ; Culture Techniques ; Fibroblast Growth Factor 2 ; biosynthesis ; genetics ; Fibronectins ; biosynthesis ; genetics ; Rats ; Rats, Sprague-Dawley ; Skin ; cytology ; metabolism ; Skin Transplantation ; Tissue Engineering ; Transplantation, Homologous

OBJECTIVEThe aim of this study was to examine the levels of prostaglandin E2 in periapical exudates before and after root canal treatment.

METHODSPeriapical exudates specimens were quantitatively sampled from root canals of 25 teeth at consecutive treatment visits, and the concentration of prostaglandin E2 in each sample was determined using a commercial kit.

RESULTSThe mean prostaglandin E2 levels significantly decreased after the endodontic therapy. Significant correlations between the levels of prostaglandin E2 and the clinical features of periapical periodontitis were also found in this study.

CONCLUSIONThe results suggest that the level of prostaglandin E2 in periapical exudates reflects the state of periapical periodontitis.

Adolescent ; Adult ; Aged ; Dinoprostone ; metabolism ; Female ; Humans ; Male ; Middle Aged ; Periapical Periodontitis ; metabolism ; therapy ; Pericardial Effusion ; metabolism ; Pulpitis ; metabolism ; therapy ; Root Canal Therapy

OBJECTIVEThe aim of this study was to evaluate quantitatively the effectiveness and feasibility of the modified supracrestal fiberotomy (MSF) and the contact point reproximation (CPR) in decreasing the relapse of anterior segments rotating and/or crowding after orthodontic treatment.

METHODSA total of 129 patients with crowding and/or rotated anterior teeth were selected for this study, and the average age was 13.07 years (54 males, 75 females). The modified supracrestal fiberotomy was performed on the anterior segments of patients in the experimental group (48 cases). After the anterior teeth were aligned, 23 of the 48 cases received a further treatment of the contact point reproximation on the anterior segments (the subgroup of MSF + CPR), and the other 25 subjects did not receive this treatment (the subgroup of MSF). The control group consisted of 81 cases. All cases wore Hawley retainers for 1.8 to 2.3 years, and all the patients were revisited 2.4 years postretention. The maxillary and mandibular dental models of all the patients were taken before treatment (T1), at the end of the treatment (T2) and 2.4 years postretention (T3).

RESULTSThe relapse rate in the experimental group [(T3-T2)/T1 x 100%] was 21.6%, lower than that in the control group (P < 0.001). The relapse rate of mandible in the subgroup of MSF + CPR was 6.56% lower than that of the subgroup of MSF (P < 0.05). But the relapse rate of maxillary in the subgroups of MSF + CPR was similar as that of the subgroup of MSF (P > 0.05).

CONCLUSIONThe modified supracrestal fiberotomy can effectively alleviate relapse after orthodontic treatment of the crowding and/or rotation of anterior teeth. The treatment combining MSF and CPR can help maintain the stability of post-retention of mandibular anterior teeth.

Adolescent ; Child, Preschool ; Female ; Humans ; Male ; Malocclusion ; therapy ; Orthodontics, Corrective ; methods ; Risk Factors ; Rotation ; Secondary Prevention ; Sex Factors ; Statistics, Nonparametric ; Tooth Extraction ; Tooth Movement Techniques ; Treatment Outcome

OBJECTIVEThe purpose of this study was to retrospect the prognosis of simultaneous repair of cleft lip and closure of cleft hard palate with vomer flaps in patients with unilateral complete cleft lip and palate.

METHODSA retrospective study was carried out in 47 patients with unilateral complete cleft lip and palate and, simultaneously received repair of cleft lip and closure of cleft hard palate with vomer flaps. The duration of operation, as well as the blood loss during the operation was recorded, and compared with those patients who only received cleft lip repair.

RESULTSAll the operations were successful, and the wound healed well. The procedure of simultaneous repair of cleft lip and closure of cleft hard palate with vomer flaps did not prolong the operating time, compared with simple cleft lip repair. No blood transfusion was needed due to closure of cleft hard palates with vomer flaps.

CONCLUSIONSimultaneous repairs of cleft lip and closure of cleft hard palate with vomer flaps are safe for patients with unilateral complete cleft lip and palate.

Abnormalities, Multiple ; surgery ; Cleft Lip ; surgery ; Cleft Palate ; surgery ; Female ; Humans ; Infant ; Male ; Maxillofacial Development ; Palate, Hard ; surgery ; Palate, Soft ; surgery ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps

OBJECTIVEThe aim of this study was to investigate the association between clinical and pathological characteristics of primary oral mucosal malignant melanoma and the prognosis of this disease.

METHODSClinical and pathological characters of 73 cases primary oral mucosal malignant melanoma were investigated. The association between risk factors, such as black macule, lymph node metastases, invasive depth, clinic stages, pathologic types, and prognosis was analyzed using Kaplan-Meier survival analysis and Log rank test.

RESULTSThe age of the patients ranged from 24 to 80 years (Median age, 50). Among the patients, 43 were males and 30 were females. The most common locations of the tumor were palate and gingiva. The clinic stages of these patients were as the following: Stage I (46/73), Stage II (24/73), Stage III (3/73). The most common pathological type was nodular (44/73), followed by lentiginose malignant melanoma (15/73) and superficial spreading (1/73). According to the configuration of tumor cells, the most common type was the mixed cell type (37/73), followed by the epitheloid cell type (27/73) and the spindle cell type (9/73). Among the 73 patients, 43 were followed up, the 3-year and 5-year survival rates were 19.86% and 11.91% respectively. Black macule, lymph node metastases, invasive depth, clinic stages, pathologic types and therapeutic methods were significantly associated with the prognosis (P < 0.05).

CONCLUSIONThe prognosis of the primary oral mucosal malignant melanoma is associated with black macule, lymph node metastases, invasive depth, clinic stages, pathologic types, and therapeutic methods.

Adult ; Aged ; Aged, 80 and over ; Female ; Follow-Up Studies ; Humans ; Lymphatic Metastasis ; Male ; Melanoma ; pathology ; Middle Aged ; Mouth Mucosa ; pathology ; Mouth Neoplasms ; pathology ; Neoplasm Invasiveness ; Neoplasm Staging ; Prognosis ; Retrospective Studies ; Survival Rate

OBJECTIVEThe aim of this study was to investigate the effects of the tympanotomy and pressure equilibrium tube insertion during palatoplasty on the alleviation of otitis media with effusion.

METHODSA total of 49 ears in 38 cases of patients with cleft palate and otitis media with effusion were only treated with palatoplasty. Other 39 ears in 24 patients with middle ear effusion were treated with the palatoplasty and insertion of pressure equilibrium tubes. The function of the middle ear in each patient was evaluated 6 months after the operation.

RESULTS48.7% of ears with middle ear effusion were healed in the group of the palatoplasty combined with pressure equilibrium tube insertion, and the hearing level was raised about 17dB six months after operation.

CONCLUSIONThe pressure equilibrium tube insertion is helpful for the recovery of the middle ear function in patients with cleft palate and middle media effusion.

Adolescent ; Child ; Child, Preschool ; Cleft Palate ; complications ; surgery ; Ear, Middle ; physiopathology ; Female ; Humans ; Male ; Middle Ear Ventilation ; Otitis Media with Effusion ; complications ; surgery ; Postoperative Complications ; prevention & control

OBJECTIVEThe aim of this study was to analyze the clinical characters, diagnosis and treatment of primary neoplasms in the parapharyngeal regions.

METHODSA total of 115 cases of primary neoplasms in the parapharyngeal regions were included in this study.

RESULTSDisorders of swallowing, breath or speaking were the common symptoms of neoplasms in parapharyngeal regions. The majority of neoplasms parapharyngeal regions were benign. 46 cases were from the deep lobe of the parotid, and 42 cases were neurogenic. CT or MRI provides useful information such as their sizes, shapes and extents. The relationship between the tumor and vessel was shown using super-selective angiography. All the tumors were resected completely by extra-oral approach through the posteroinferior incision of the mandible. The follow-up was carried out from 1 to 10 years, and the data indicated that 2 patients died, 18 cases were lost, and others had no recurrence.

CONCLUSIONCT and/or MRI are essentially useful for diagnosis and presurgical planning. Super-selective angiography can be used if the lesions involved carotid artery. Posteroinferior incision of the mandible is a simple, effective and safe approach for excision of neoplasms parapharyngeal regions.

Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Follow-Up Studies ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Neurilemmoma ; diagnosis ; pathology ; surgery ; Parotid Neoplasms ; diagnosis ; pathology ; surgery ; Pharyngeal Neoplasms ; diagnosis ; pathology ; surgery ; Retrospective Studies ; Tomography, X-Ray Computed

OBJECTIVEThe purpose of this study was to determine the effects of amino group implantation on the surface structures of titanium.

METHODSThe amino groups were implanted into the surfaces of titanium using 100 keV amino group ions at ambient temperature (25 degrees C) with the following three different doses: 5 x 10(17), 1 x 10(17) and 5 x 10(16) ions per cm2. The current density of the ion-beam was (10 microA/cm2. The modified surfaces were characterized using X-ray photoelectron spectroscopy (XPS) to determine the chemical components of the modified surfaces. A scanning electron microscope (SEM) was used to study the physical structure of the surfaces.

RESULTSThe wide XPS profiles, the binding energy data and the SEM photos were obtained. The XPS showed that the modified surface contained Ti, O, C and N. The possible chemical states were speculated using Gauss matching analysis. There was no significant difference between the surfaces with and without implanted amino groups.

CONCLUSIONThe XPS data reveals that the structure of the modified surface layer of aminogroup-implanted titanium is TiN, comprising a complex structure of titanium and amino compound near the surface. The SEM Photos show that this technique has little effect on the surface structures of materials.

Amines ; chemistry ; Biocompatible Materials ; chemistry ; Materials Testing ; Surface Properties ; Titanium ; chemistry ; X-Ray Diffraction