2.Dgcr8 deletion in the primitive heart uncovered novel microRNA regulating the balance of cardiac-vascular gene program.
Xi CHEN ; Lin WANG ; Rujin HUANG ; Hui QIU ; Peizhe WANG ; Daren WU ; Yonglin ZHU ; Jia MING ; Yangming WANG ; Jianbin WANG ; Jie NA
Protein & Cell 2019;10(5):327-346
Primitive mammalian heart transforms from a single tube to a four-chambered muscular organ during a short developmental window. We found that knocking out global microRNA by deleting Dgcr8 microprocessor in Mesp1 cardiovascular progenitor cells lead to the formation of extremely dilated and enlarged heart due to defective cardiomyocyte (CM) differentiation. Transcriptome analysis revealed unusual upregulation of vascular gene expression in Dgcr8 cKO hearts. Single cell RNA sequencing study further confirmed the increase of angiogenesis genes in single Dgcr8 cKO CM. We also performed global microRNA profiling of E9.5 heart for the first time, and identified that miR-541 was transiently highly expressed in E9.5 hearts. Interestingly, introducing miR-541 back into microRNA-free CMs partially rescued their defects, downregulated angiogenesis genes and significantly upregulated cardiac genes. Moreover, miR-541 can target Ctgf and inhibit endothelial function. Our results suggest that microRNAs are required to suppress abnormal angiogenesis gene program to maintain CM differentiation.
3.Correction to: Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg mice.
Yaqin DU ; Ting WANG ; Jun XU ; Chaoran ZHAO ; Haibo LI ; Yao FU ; Yaxing XU ; Liangfu XIE ; Jingru ZHAO ; Weifeng YANG ; Ming YIN ; Jinhua WEN ; Hongkui DENG
Protein & Cell 2019;10(2):154-155
In the original publication Fig. 1D and supplementary material is incorrect. The correct figure and supplementary material is provided in this correction.
4.Chemical screen identifies a geroprotective role of quercetin in premature aging.
Lingling GENG ; Zunpeng LIU ; Weiqi ZHANG ; Wei LI ; Zeming WU ; Wei WANG ; Ruotong REN ; Yao SU ; Peichang WANG ; Liang SUN ; Zhenyu JU ; Piu CHAN ; Moshi SONG ; Jing QU ; Guang-Hui LIU
Protein & Cell 2019;10(6):417-435
Aging increases the risk of various diseases. The main goal of aging research is to find therapies that attenuate aging and alleviate aging-related diseases. In this study, we screened a natural product library for geroprotective compounds using Werner syndrome (WS) human mesenchymal stem cells (hMSCs), a premature aging model that we recently established. Ten candidate compounds were identified and quercetin was investigated in detail due to its leading effects. Mechanistic studies revealed that quercetin alleviated senescence via the enhancement of cell proliferation and restoration of heterochromatin architecture in WS hMSCs. RNA-sequencing analysis revealed the transcriptional commonalities and differences in the geroprotective effects by quercetin and Vitamin C. Besides WS hMSCs, quercetin also attenuated cellular senescence in Hutchinson-Gilford progeria syndrome (HGPS) and physiological-aging hMSCs. Taken together, our study identifies quercetin as a geroprotective agent against accelerated and natural aging in hMSCs, providing a potential therapeutic intervention for treating age-associated disorders.
6.Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants.
Lei YANG ; Xiaohui ZHANG ; Liren WANG ; Shuming YIN ; Biyun ZHU ; Ling XIE ; Qiuhui DUAN ; Huiqiong HU ; Rui ZHENG ; Yu WEI ; Liangyue PENG ; Honghui HAN ; Jiqin ZHANG ; Wenjuan QIU ; Hongquan GENG ; Stefan SIWKO ; Xueli ZHANG ; Mingyao LIU ; Dali LI
Protein & Cell 2018;9(9):814-819
7.Effective and precise adenine base editing in mouse zygotes.
Puping LIANG ; Hongwei SUN ; Xiya ZHANG ; Xiaowei XIE ; Jinran ZHANG ; Yaofu BAI ; Xueling OUYANG ; Shengyao ZHI ; Yuanyan XIONG ; Wenbin MA ; Dan LIU ; Junjiu HUANG ; Zhou SONGYANG
Protein & Cell 2018;9(9):808-813
Adenine
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Animals
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Gene Editing
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Mice
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Zygote
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metabolism
8.Correction to: Prof. Huan-Yong Chen: a leading botanist and taxonomist, one of the pioneers and founders of modern plant taxonomy in China.
Protein & Cell 2019;10(5):388-388
In the original publication, there are some incorrect information.
10.A tribute to my supervisor Professor Zhengyi Wu.
Protein & Cell 2018;9(10):827-831