The general requirement of the Central Committee of the Communist Party of China is to make 'a project of satisfying the people' with the education on keeping the advanced nature of the Party members.To carry out this in a hospital of the army, we must ①focus on the ideological characteristics of the staff, overcome the ideas such as valueing business more than politics, having nothing to do with oneself, being complacent or indifferent that exist in the minds of some members, and lead all the Party members to actively join the educational activities; ②focus on the function and mission of a army hospital, make solid medical and logistic preparation for military struggle, and keep improving the quality and standard of medical service; ③focus on the characteristics of routine work interweaved with multiple threads and high intensity, carry out the education with aborative planning and feasible boosting.

Objective: To explore the value of integrated backscatter(IBS) of kidney with different dosage of organophosphorus in rabbits. Methods: Animal models of organophosphorus poisoning were established. Ultrasonographic examinations were performed on four different dosage experimental groups and control group at 1h and 3 h after rabbits being poisoned.The echo and the size of kidney were evaluated by two-dimensional ultrasound.The changes of parenchyma were quantitatively analyzed by IBS.Results: No significant changes were found in kidney echo of any groups.With the increasing dosage and prolonging time of poisoning,significant changes were found in volume and length of kidneys comparing with that of control group(P

Objective:To investigate the role of transcription factor SP1 decoy oligodeoxynucleotides(ODN) on expression of ? Gal in SV-40-PED cells.Methods:Immortalized porcine aortic endothelial cells of the PED line were cultured and transfected with ?1,3galactosyltransferase(?1,3GT) specific decoy ODN.Cells transfected with mismatch ODN was used as negative controls.Twenty-six hours later the cells were collected.The expression of ? Gal was determined with fluorescence microscope and Western blot.The expression of ?1,3GT mRNA was examined by RT-PCR.Results:Fluorescence microscopy observed the decreased fluorescence of ? Gal after decoy ODN transfection.Western blot showed that the average absorbance of the PED cells transfected with decoy ODNs was(48.2?0.9).It is 52.6% of the mock group(P0.05).Conclusion:?1,3GT gene reduce actually occurs following transfection of decoy ODN.Porcine endothelial cells can be the targets of decoy ODN.

Objective:To compare the effects between estrogen and Prasterone(Dehydroepiandrosterone,DHEA) on improving atherosclerosis of ovariectomized rabbits. Methods: Thirty female New Zealand white rabbits of three months old were randomly divided into five groups: Group A: normal control;Group B: sham operation + high cholesterol feeding;Group C: ovariectomized + high cholesterol feeding;Group D: ovariectomized + high cholesterol feeding+ estradiol benzoate;Group E: ovariectomized + high cholesterol feeding+ DHEA.Twelve weeks later,all rabbits were sacrificed,the serum TC,TG,LDL-C,HDL-C,NO and estradiol levels were measured.The aortas and uterus were harvested for histomorphometrical evaluation with light and scanning electron microscope.The area ratios of atherosclerotic lesion to endothelium,and the thickness and area ratios of endothelium to the medium were determined by computer. Results: ①Following the treatment for 12 weeks,no significant difference was found in serum TC、LDL-C、HDL-C levels among four groups except in group A.②There were significant differences between the serum triglyceride(TC)levels of group D and group C.③The serum No levels of group D and group E were significantly higher than that of groupC.④The serum estradiol levels of group D and group E were significantly higher than that of groupC.⑤The area ratios of atherosclerotic lesion to endothelium and the thickness and area ratios of endothelium to the medium in group D and group E were significantly lower than that of group C.⑥Endometrial hyperplasia of group D was significant,while there was no such Phenomena in group E. Conclusion: Estrogen and DHEA can alleviate the early pathological changes of atherosclerosis of the ovariectomized rabbits,prevent and reduce the formation of atherosclerosis.

Objective:Currently the treatment model of lumbar intervertebral disc herniation(LIDH) has changed from single surgical treatment to multisubjective comprehensive treatment.The purpose of this study was to observe the curative effect of synergic therapy with traditional Chinese medicine and physical therapy on LIDH.Methods:86 cases of LIDH treated with drawing,massage,Chinese herbal medicine injection and TDP radiation.Results:All the patients were followed for 1-7 months(mean 3 months).Convalescence 56 cases(65.1 %),effective 28 cases(32.6 %),no effect 2 cases(2.3 %),the total effective rate was 97.7%.Conclusion:The synergic therapy with traditional Chinese medicine and physical therapy was easy,safe and effective for LIDH.

0.05),but the positive degrees were distinctly different(P0.05). Conclusion:The status of p53 may be related with multidrug resistance and the p53 positive tumors possibly show low sensitivity to chemotherapy.

Objective:To investigate the expression of epidermal growth factor receptor(EGFR),survivin,P53 protein and proliferating cell nuclear antigen(PCNA) in thymic carcinoma and type-B3 thymoma. Methods:The clinicopathological changes of thymic carcinoma in 9 cases and of type-B3 thymoma(well differentiated thymic carcinoma) in 21 cases were examined by microscopic observation of immunohistochemical stained EGFR,survivin,P53 protein and PCNA,respectively. Results:Clinically,the median age of the patients with the tumor was 43.5 years old.All the tumors displayed a pattern of infiltration growth.Immunohistochemical staining of the tumors indicated that the expressions of EGFR,survivin,P53 protein and PCNA in thymic carcinoma were 77.8%(7/9),88.8%(8/9),77.8%(7/9) and 100%(9/9),respectively.Meanwhile,in type-B3 thymoma,they were 67.7%(14/21),71.4%(15/21),33.3%(7/21) and 85.7%(18/21),respectively.It was statistically significant that the immunohistochemical phenotypes of the tumor displayed a different reaction for P53 protein. Conclusion:The thymic carcinoma and type-B3 thymoma may possess a complete invasive characteristic,clinically.To discriminate thymic carcinoma and type-B3 thymoma,the expression of P53 protein is a more valuable index than EGFR,survivin or PCNA.

Objective:To investigate the therapeutic effects of ultrasound irradiation microbubble agent in the treatment of subcutaneous H22 ascites cells hepatoma of mice.Methods:H22 ascites cells hepatoma models were established by inoculating tumor cells to subcutaneous tissue of mice.Then,mice were randomly allocated into control group,only ultrasound group,ultrasound and microbubble group.The growth of tumor volume was observed and its weight was calculated.The application of pathological examination was adopted after the treatment.The necrosis area percentage(NAP) was calculated.and the apoptotic index was analyzed with terminal deoxynucleotidyl transferase mediated dUTP nick end lebeling(TUNEL) technique.Results:Compared with the control,the growth of tumor in the ultrasound and microbubble group was slow and the inhibitory effect of tumor was obvious;Necrosis area and apoptotic cells number were also increased obviously(P

Objective: To detect the expression and distribution of activated cytotoxic cells in types of lymphoma with tissue microarray,and provide evidences for clinical treatment and prognosis. Methods: Immunohistochemical staining by S-P technique was used for detecting the expression and distribution of perforin and granzyme B in lymphoma tissue microarray,composed of 60 samples of lymphoma tissue.10 NK/T-cell lymphoma routine sections were used for relative research,and 10 reactive hyperplasia were used for comparison. Results: In the tissue microarray,samples originated from intranode and extranode were 48 and 12,respectively;consisting of 42 B-cell lymphoma,16 T-cell lymphoma(10 PTCLs,2 NK/T-cell lymphomas,2 lymphoblastic T-cell lymphomas,2 anaplastic large cell lymphomas),2 Hodgkin's disease.42 samples of B-cell lymphoma cells were negative in perforin and granzyme B.In 10 samples of peripheral T-cell lymphoma,perforin and granzyme B positive were 8 and 9,respectively,but the positive cells were no tumor cells.In 12 samples of NK/Tcell lymphoma(2 in the tissue microarray,10 routine sections),both perforin and granzyme B were strongly positive.B-cell lymphoma,T-cell lymphoma and NK/T-cell lymphoma differed significantly(P

Objective:To study the relationship between the cell density and the cell proliferation phenotype. Methods: Plate clonality assays was used to measure the impact of cell density to cell clonality and cell cycle in BT325、786-0、293、C6 and NIH3T3 cell lines. Results: The clonality decreased when the cells grown to confluence in NIH3T3 and 7860 cell lines respectively.It seem need more cells to decrease the clonality in 293 cell line but there is no relationship between cell density and cell clonality in BT325 and C6 cell lines.Cell cycle analysis show that cell density have no effect on BT325 and C6 but on 786-0、293 and NIH3T3 cell lines. Conclusion: There might exist preventer or preventers,which is proportional to the number of cells,of immortal stem cell to expand.In addition,the rate of stem cell expansion is proportional to that of cell mitosis in immortal cell lines.