1.Evaluation of the malaria control program in Vietnam in the period 2000-2007.
Journal of Malaria and parasite diseases Control 2003;0(1):3-12
Background: National malaria preventive and control program is rather successful, but the special result has not been accurately analyzed.\r\n', u'Objectives: to evaluate of the malaria situation for the period of 2000-2007 in Viet Nam.\r\n', u'Subjects and methods: This retrospective study analyzed data from malaria information system.\r\n', u'Results: On annual average, about 140.000 to 150.000 patients are diagnosed as malaria. Annual malaria morbidity is greatly reduced from 6.91/1000 in 2000 to 2.21/ 1000 population in 2007. The deaths in children accounted for 30 to 40% of the total malaria deaths of the whole country. The annual average number of malaria cases treated is about 150.000 to 160.000 and it is decreased by year. Artemisinine based Combination Therapy (ACT) for P.falciparum cases is 18 - 20% of the total patients. \r\n', u'From 2000 to 2007, on average, about 1.5 to 2 million of people in the malaria endemic, high risk areas were protected by Indoor Residual Spray (IRS) annually. The coverage of protected population tends to be reduced from 6.7% to 5.9 % of population at risk of malaria. \r\n', u'The annual average number of population protected by Insecticide Treated Bed Nets (ITNs) is from 8.5 to 11.5 million and the coverage of ITNs is increasing from 23.6% to 31.0% of population in malaria endemic areas. . \r\n', u'Conclusion:From 2000 to 2007, the malaria in Vietnam continues to be decreased in term of morbidity and mortality.\r\n', u'
Malaria control program
;
evaluation
2.Evaluate on epidemic supervision and propose solutions to protect healthy for soldiers and people in malaria epidemic area.
Ngo Van Ly ; Thuan Khanh Le ; Loc Ba Ly ; Doan Van Nguyen ; Sinh Cong Nguyen ; Tuan Dinh Trinh ; Duc Ai Quach ; Thao Duc Nguyen ; Thuc Duy Nguyen ; Binh Van Nguyen ; Thang Duc Do
Journal of Malaria and parasite diseases Control 2003;0(1):13-24
Background: Coordinating between soldiers and people bring significant results in protecting healthy for soldiers and people in malaria epidemic area.\r\n', u'Objectives: To evaluate epidemic supervision and propose solutions protecting healthy for soldiers and people in malaria epidemic area.\r\n', u'Subjects and methods: A retrospective cross sectional study was carried out on two provinces Binh Phuoc and Dak Lak\r\n', u'Results: there was 4 main disease types were fever, tuberculosis, diarrhea and malaria. Malaria at investigated times still accounted high rate comparing with general fever rate. Malaria risk contained in people living or working in forest and mountain field; free emigrants; armed forces and border guard. These subjects needed to improve regularly protection from malaria. Communication of health education is one of important method to prevent and control malaria.\r\n', u'Conclusion: It is necessary to implement strong methods (such as providing insecticide treated bed nets, indoor residual spray) to prevent and control malaria. \r\n', u'
Epidemic supervision
;
healthy
;
soldier
;
malaria epidemic area.
3.Some results of malaria parasite species collected from Daknong province and analysis of drug resistance in P.Palciparum by the polymerase chain reaction.
Nhan Hanh Doan ; Duong Van Nguyen ; Thanh Viet Ngo ; Toan Quoc Tran ; Phuong Nhu Vo
Journal of Malaria and parasite diseases Control 2003;0(1):25-30
Background: In many years, National Institute of Malariology, Parasitology and Entomology conducted collection, storage and preservation of malaria parasites species \r\n', u'Objective: to evaluate some results of malaria parasite species collected from Daknong province and analysis of drug resistance in P.Palciparum by the polymerase chain reaction.\r\n', u'Subject and method: Malaria parasite species collected from Daknong province in 2006. Thirty-five isolates were confirmed to be resistant with chloroquin by in vitro test. Polymerase chain reaction-restriction fragment leng polymorphism were used. \r\n', u'Results: 55 Plasmodium jalciparum. 7 Plasmodium vivax. 4 Plasmodium malariae. 1 Plasmodium ovale samples were collected from the malaria patients. A preliminary analysis of drug-resistant mutations in the Plasmodium jalciparum chloroquine resistance transpory (pfcrt) and P Jalciparummulti-drug resistant genes showed that the change of the order of amino-acid of Plasmodium jalciparum was closely correlated to chloroquine resistance in 35 isolates at the mutant allele 76 of pfcrt gene of chloroquine resistant Plasmodiuntjalciparum isolates. \r\n', u'Conclusion: These results contributed to supplement malaria parasite species that were stored in National Institute of Malariology, Parasitology ad Entomology.\r\n', u'
Malaria parasite species
;
polymerase chain reaction
;
P.Palciparum
;
drug resistance
4.Resistant to insecticides of three mosquito species: Aedes Aegypti, Culex quinquefasciatus and culex tritaenyorhynchus in Ha Noi
Khoa Thi Pham ; Phong Ba Nguyen ; Ha Thanh Pham ; Bien Thi Vu ; Trang Thi Thu Nguyen ; Khiet Minh Doan
Journal of Malaria and parasite diseases Control 2003;0(1):31-36
Background: Currently, tracking, monitoring insecticide resistance of mosquitoes transmitting disease is an urgent need.\r\n', u'Objectives: To evaluate resistance to insecticide of three mosquito species: Aedes Aegypti, Culex quinquefasciatus and culex tritaenyorhynchus in Ha Noi\r\n', u'Subject and method: Aedes Aegypti, Culex quinquefasciatus culex tritaenyorhynchus mosquito species was collected from some districts of Ha Noi city. These mosquito species was tested sensitivity with 4 insecticides: permethrin 0.75%, lambda-cyhalothrin 0.05%, alpha-cypermethrin 30mg/m2 and DDT 4.0%.\r\n', u'Results: Three main vectors of Dengue fever, Japanese Encephalic and Filariasis mosquitoes were found in a very high density in some areas of Ha Noi City. Aedes aegypti mosquitoes in the districts of Thanh Xuan (TX), Dong Da (DD) and Hoang Mai (HM) was found highly resistant to permethrin, alpha - cypermethrin, lambda - cyhalothrin and DDT with the mortalities rates of 51 % (TX) and 59%(DD); 70%(HM); 90%, 82% and 96%; 74%, 54% and 59%; 1 %, 8% and 32%, respectively. \r\n', u'Culex tritaenyorhynchus was found also highly resistant to permethrin with the mortality rate of 29%, alphacypermethrin - 18%, lambdacyhalothrin - only 3% and DDT x36%. Culex quinquefasciatus was found also highly resistant to those insecticides. \r\n', u'Conclusion: Other insecticides should choose to replace the chemicals used in this experiment to control, kill mosquitoes when epidemics occur.\r\n', u'
Insecticides
;
resistance
;
Aedes Aegypti
;
Culex quinquefasciatus
;
culex tritaenyorhynchus
5.Result of investigation on medical arthropod in some place along Ho Chi Minh road in Cao Bang province
Chau Van Nguyen ; Bich Xuan Phung ; Hien Thi Do ; Kha Thi Nguyen ; Lien Thi Huong Nguyen ; Huong Van Hoang
Journal of Malaria and parasite diseases Control 2003;0(1):37-46
Background: In the period 2006-2010, a investigation on medical arthropod has been conducted\r\n', u'Objective: 1) To determine species composition and distribution of medical arthropod. 2) To find species transmitted disease \r\n', u'Subject and method: The cross sectional, descriptive and analytic investigation was conducted in 3 communes along the Ho Chi Minh road in Cao Bang province. \r\n', u'Results and conclusion: : A total of 3.437 of samples of medical arthropod belonging to 96 species have been collected including: three species of flea (Siphonaptera), two species of tick (Ixodoidea), four species of chigges (Trombiculidae), ten species of mites (Gamasoidea), twenty four species of flies (Muscoidea), and fifty-three species of mosquito (Culicidae). They belong to 39 genuses, 17 families, 3 orders: (Siphonaptera, Acarina and Diptera); two classes (Insecta and Arachnida). \r\n', u'Some species having epidemiological role such as Aedes albopictus, Culex tritaeniorhyncus, Cx. quinquefasciatus, Cx. vishnui ... were predominant at all study sites. Anopheles minimus, the main malaria vector, was mainly collected in cattle- sheds in Truong Ha and Bach Dang communes. \r\n', u'
Medical arthropod
;
investigation
6.Trematode infection situation in Nghia Phu and Nghia Lac communes in Nghia Hung district, Nam Dinh province, 2007
Thach Thi Cam Dang ; Dung Trung Do
Journal of Malaria and parasite diseases Control 2003;0(1):47-53
Background: Diseases due to parasites transmitted through food, including liver fluke and intestinal fluke a dangerous disease groups for people.\r\n', u'Objectives: to evaluate trematode infection situation in epidemic areas before providing drug to treat massively high-risk subjects.\r\n', u'Subjects and methods: A cross-sectional study was carried out in two communes of Nghia Hung district, Nam Dinh province to determine the trematode infection in human in August 2007. Families were randomly selected, and all of members of households were examined for trematode eggs by Kato-Katz technique. A total of 578 people, comprising 321 males (55.5%) and 257 females (44.5%) underwent stool examination. T\r\n', u'Results:120 (37.4%) males and 66 (25.7%) females were positive for small trematode eggs. There was a significant difference between males and females for infection rates with small trematode (X2 = 8.43; p < 0.05). The infection rate of trematodes was lowest in \u2264 20 age group and highest in >60 years old among both males and females. In the majority of the patients with small trematode eggs, the infection intensity was low: about 90.3% whereas 9.7% had moderate infection intensity. High intensity rate (>10.000 epg) was not found. \r\n', u'Conclusion: Trematode infection situation through food is still a healthy problem at investigated epidemic areas. \r\n', u'
Trematode infection
;
7.The profile of airborne fungi at a poultry market
Anh Tran Le ; Thuy Ngoc Nguyen ; Tuan Quoc Le
Journal of Malaria and parasite diseases Control 2003;0(1):54-61
Background: Ha Vi poultry market is a concentrated and spontaneous poultry business place. Ensuring environmental hygiene is not respected.\r\n', u'Objectives: To evaluate infectious situation of airborne fungi at a poultry market\r\n', u'Subjects and methods: From 10/2006 to 9/2007 a survey on the profile of airborne fungi was carried out at the Havi poultry - market. Total fungal spores averaged at 2.753 \xb1 576 cfu/m3, the highest sample is 7.120 cfu/m3\u2022 More than 10 different fungi were isolated. \r\n', u'Results: The common fungi are Cladosporium (30.13%), Penicillium (26.86%), Aspergillus (20.97%, composed of A.niger, A fumigatus, A.jlavus, A.clavatus ...), Fusarium (8.15%), some rare fungi (Rhizopus, Rhizomucor, Mucor, Alternaria, Curvularia, Absidia, Bipolaris ... ) 13.89%. The concentration of airborne fungi at the market was higher than at distance of 300 and 1000 m. The concentrations of total fungi and of each species are affected by some meteorological factors: they were higher in the end of spring, early of summer and autumn, lowest in the winter. The concentrations of Cladosporium, Penicillium were found to be positive correlate with humidity, Aspergillus, Fusarium with temperature. \r\n', u'Conclusion: It is necessary to propose the methods that can reduce influence of airborne fungi pollution to health of people \r\n', u'
Airborne fungi
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Cladosporium
;
Penicillium
;
Aspergillus
;
Fusarium.
8.Identification of taenia spp adults and cysticercose cysts in human by using multiplex PCR
Vien Viet Ha ; Dao Due Le ; Manh Due Nguyen ; Tan Van Hoang ; Nguyen Hanh Doan ; Nhung Thi Vu
Journal of Malaria and parasite diseases Control 2003;0(1):62-69
Background: The multiplex polymerase chain reaction can rapidly differentiate taenia spp adults and cysticercose cysts.\r\n', u'Objective: The study aimed to identify taenia spp adults and cysticercose cysts in human by using multiplex PCR\r\n', u'Subjects and methods: The multiplex PCR was applied by using the 4 forward and one reverse primer to amplify the target gene cytochrome c oxydase subunit I (COXI) of the Taenia spp in human. T\r\n', u'Results:4 molecular sizes of PCR products were appeared: 269 bp,720 bp, 827 bp, 984 bp. Seventy six samples including 65 flat worms and 11 cysticercose cysts which collected from the patients who are living in 19 different provinces and cities of Northern part of Viet Nam and treated in the clinic of NIMPE were examined. Of 65 flat worms analyzed 35 samples were T.asiatica (58.46%), 27 were T.saginata (41.54%). All 11 cysticercose cysts were T.solium (100%). \r\n', u'Conclusion: The result also indicated that some time 3 Taenia species were found at the same area. A remarkable difference of infection rate was found between men and women, also adults and children. \r\n', u'\r\n', u'
Taenia spp adults
;
cysticercose cysts
;
multiplex PCR
9.Situation of clonnorchis sinensis infection in workers of tea companies in Phu Tho province year 2007 and evaluating efficacy of specific intervention
Journal of Malaria and parasite diseases Control 2003;0(1):70-75
Background: Phu Tho is a mountainous province in the northwest of Vietnam. People here have the habit to eat raw fish; this is the favorable conditions for small liver fluke (clonorchsis sinensis) circulation and development. \r\n', u'Objectives: 1) To determine infection rate and infection intensity in workers of tea companies in Phu Tho province. 2) To evaluate effectiveness of treatment Clonorchis sinensis by Distocid.\r\n', u'Subjects and methods: Across- sectional survey was carried out in September 2007 in the tea companies in Phu Tho province, 400 peoples (200 male, 200 female), were examined stool samples to find helminthic eggs by Kato-Katz technique.\r\n', u'Results: The cumulative infection rate Clonorchis sinensis was 22.25%, of which a significant difference of the infection rate of clonorchis sinensis was found in males: 27.4% and in females: 16.7%, respectively (p< 0.05). The cure rate by Distocid was 97.7%.\r\n', u'Conclusion: The infection rate of workers in the areas was rather high\r\n', u'Therefore, it is necessary to propagandize and educate for workers and the community understanding of the harmful effects of Clonorchis sinensis and how to prevent small liver fluke disease; changing risk behaviors such as not eating raw fish\r\n', u'
Clonnorchis sinensis
;
workers
;
tea companies
10.The sensitivity, specificity and diagnosed conformity of Rapid Diagnosis Test (Paracheck F test) compared with microscopic method for detecting Plasmodium falciparum
Tinh Thi Ta ; Hai Thanh Nguyen ; Ba Thanh Truong ; Thanh Kim Nguyen ; Thuan Khanh Le
Journal of Malaria and parasite diseases Control 2003;0(1):76-81
Background: Paracheck F.test is rapid diagnostic test that use to determine presence of histidine rich protein 2-HPR-2, a component of Plasmodium falciparum in total blood. Paracheck F.test producted by India\r\n', u'Objective: To study the sensitivity, specificity and diagnosed conformity of Rapid Diagnosis Test (Paracheck F test) compared with microscopic method for detecting P.falciparum \r\n', u'Subject and method: The study was conducted in 5 communes of Quang Tri province and 1 commune of Quang Binh province from September to November in 2006. A total of 470 blood samples were collected and examined with 2 methods for detecting P falciparum (Microscopy and Rapid Diagnosis Test- Paracheck). \r\n', u'Results:Among these blood samples, there were 438 samples having complete conformity in both two methods. 29 samples were negative with microscopy but positive with Paracheck. 3 samples were positive with Paracheck but negative with microscopy.\r\n', u'Conclusion: the sensitivity and specificity of Paracheck is 99% and 82%, respectively. Both microscopic and Paracheck methods have a high conformity in detecting P falciparum with the Kappa index of 0.84. \r\n', u'
Sensitivity
;
specificity
;
diagnosed conformity
;
Paracheck F test
;
microscopy