Objective:To observe and compare the inhibitory effect of two fluor protectors with different concentrations on man-made dental caries of human enamel smooth surface and socket in vitro.Methods:Forty smooth surfaces and forty sockets non-carious human enamels were divided into four groups randomly.Each group had ten samples,five samples were employed to experiment,and five samples were used as spare ones.For experiment group(0.5%fluor protector,0.1% fluor protector).the dental enamel surfaces were spreaded 0.5% fluor protector and 0.1% fluor protector twice in series respectively.For positive control group(0.05% NaF),the dental enamels were dipped into 0.05% NaF for 24h,then taken out and dried.For blank control group(deionized water),the dental enamels were immersed into deionized water for 24h,then taken out and dried.The specimens were taken out of when they had been immersed into man-made cariogenic fluid(37℃)for 168h,rinsed and dried, then they were divided into two halves by high speed corundum disk in spurting water;one half was used to observe depth of dental caries by polarization microscope,and the other half was employed to observe surface demineralization by scanning electron microscope.Results:The dental Caries depth of 0.5% fluor protector group was the smallest in four groups,and there were significant differences between 0.5% fluor protector group and 0.1% fluor protector group(P

Objective:To probe the effect of noise on the capabilities of learning and memory of growing rat and its possible mechanism.Methods:24 Wistar growing rats were divided randomly into two groups,noise group and control group.The growing rats in the noise group were exposed to 90dB(A) noise constantly for one month.The capabilities of learning and memory of growing rats were measured by means of Morris water maze test.An immunohistochemical method was used to observe the expression of aminobuty-ric acid(GABA) positive neurons in hippocampus.Results:The test of Morris water maze showed that the mean escape latency of growing rats in the noise group were longer than that in the control group and the frequency to bestride platform were less.The number of GABA positive neurons and the magnitude of tinction in hippocampus of growing rats in the noise group were reduced markedly than that in the control group.Conclusion:The noise does impair the capabilities of learning and memory of growing rats.The impairment may be related with the reduction of GABA contents in hippocampus,which could decrease the depressive effect of neuronic synapse and impair the induction and maintenance of the long term potentiation.

0.05).The expressions were remarkably of endometrial ER、VEGF and KDR in the study group as extremely lower than those in the control group,and the statistical difference between the two groups were all significant(P0.05).Conclusion:ER、VEGF and KDR may be relevant to the occurrence of hypomenorrhea.

Objective:To discuss the depressed expression of BUB1 gene in trophoblastic cells by microRNA.Methods:Endogenous BUB1 mRNA level was compared by using quantitative real-time PCR before and after transfected with microRNA plasmid; and the Bub1 protein level was compared by western blot.Results:Ratio of BUB1 mRNA/GAPDH mRNA of control group was 0.1960?0.0688,and after transfected with microRNA recombinant plasmid was 0.1968?0.0689 and 0.1962?0.0670 respectively.Statistical study of samples showed they have no instinct different;otherwise the protein level was significantly decreased after transfected with microRNA plamid.Conclusion:This study established microRNA can control gene expression in mammalian cells at translation level,it is a good foundation to study on the control of gene expression.

A follow-up observation of 222 HBsAg carriers was carried out over four years. The serological results showed that 46 out of 222 HBsAg carriers were negative converted (20.7%) , 25 out of 72 HBeAg positive carriers were negative converted (34.72%). The clinical results showed that 12 suffered from acute hepatitis, 10 from chronic persistent hepatitis (CPH), 8 chronic active hepatitis(CAH), 3 hepa-tocirrhosis, 1 primary hepatic carcinoma (PHC), 25 with slight liver function damage, 117 were asymptomatic carriers, and 46 persons recovered.This follow-up study indicated that the negative-conversion rate of HBsAg carriers with high titer or HBeAg positive was significantly lower than those with low titer or HBeAg negative and that the negative-conversion rate of HBsAg carriers or HBeAg positive increased with age

S'. rum sialic acid(SA), fucost(Fuc) and five acute phase proteins were measured in 55 patients with digestive tract cancer(DTC), 61 with digestive tract bengin diseases and 58 hcalihy blood donors. The serum concentrations of SA, Fuc, ?1-acid glycoprotcin(?1-AG), ?1-antitrypsin(?1-AT) and haptoglobin (HP) were significantly elevated in the patients with cancer as compared with the control group(P

A new technigue of the macroscopic vasovasostomy has been reported. The procedure was carried out by suturing only tunic of the both ends of the vas with catgut to splint and reduce tension(Group 1 ).In animal experiment, this technigue was compared with following methods of vasovasostomy. ( 1 ) .tunic-muscular layer anastomosis without splint(Group 2),(2).whole layer anastomosis with stainless steel splint (Group 3) and (3).whole layer anastomosis without splint. Among all dogs,the operative procedure was performed without the aid of optical magnification nor any special instruments. The four technigues were tested in 60 dogs (120 vasa) in which each of them used 15 dogs (30 vasa). The results of the dog experiments showed that the anatomical patency rates of the Group 1,2,3 and 4 after operation were 63.3%, 66.7%, 23.3%and 10%.Our studies indicate that the use of absorble catgut as an intravasal splinting and reducing tension in vasovasostomy is feasible in dogs.Gross as well as microscopic evaluation of the anastomotic area demonstrated that the intraluminal material was substantially absorbed with nosignificant reaction after operation.As compared with other macroscopic vasovasostomies, this technic is claimed to be simpler, easier, less traumatic, effective and feasible

The following indexes of 2, 10 and 24 months old wistar rats were measured. the activities of RBC membrane Na+-K+-ATPase, RBC superoxide dismutasc (SOD), blood glutathione. peroxidase (GSH-Px), and the levels of lipid peroxides (EPO) in plasma and RBC membrane From 2 to 10 month-old animals, increases of 24.16%,40.07%, 102.7% and 44.44%in Na-K-ATPase activity, GSH-Px activity, plasma LPO and RBC membra no LPO levels, respectively, were found (P

Objective:To search a dissolvent which can relive the pain for intramuscular injection of penicillin.Methods:100 pa- tients were randomly divided into two groups,each had 50 patients.all individuals had normal aesthema and speaking ability,the serious failure ones and patients who had used sedatives or pain-killer recently were not brought into the study.Use 0.9% sodium chloride solution as dissolvant for intramuscular injection of penicillin in experimental group,Sterile Water for Injection in the control group.Observe the subjects'focal pain and general reaction after injected.Results:subjects injected with 0.9% sodium chloride solution as dissolvant had less pain and general reaction,the rate of acupuncture syncope was reduced.Conclu- sion:Use 0.9%sodium chloride solution as dissolvant for intramuscular injection of penicillin can efficaciously relieve patients' focal pain and general reaction,reduce the rate of acupuncture syncope.It's a safe dissolvant.

Objective:To establish normal Delaire Architectural Analysis database of the Han nationality adults with normal occlusion in Chongqing.and to explore their cranio-maxillo-facial structure features.Methods:Seventy-eight Han adults with normal occlusion(male 37,femal 41)aged from 18 to 26 years were included.All adults were with normal and well-balanced faces,did not receive orthodontic or orthonathic treanment.The lateral cephalogram of each normal adults were traced,digitized, measured by Winceph8.0 software.Statistical calculation performed cephalometric values was established,compared with their craniofacial structure characteristics with Korean's.Conclusion:To establish the normal database of cranio-maxillo-facial structure of Chongqing adults with Delaire architectural analysis.