Objective To assess overall diagnostic value of SELDI-TOF-MS in early breast cancer. Methods To search and identify the relevent articals in English and Chinese from Jan. 1999 to Dec. 2007 on Medline,WeiPu full-text data base and Wan-Fang clara base, and the literatures have been integrated to review the quality rating, and the heterology examination and the meta analysis were conducted. While the summary receiver operating characteristic curve (SROC), pooled sensitivity, specificity and odds rate were calculated by SPSS13.0 software. Results 22 pertinent literatures can be indexed as a whole, of which 8 articles in-cluding 739 samples entered this Meta analysis. The pooled sensitivity, specificity and odds rate were 91.4% ,99.5% and 30.88 re-spectively. The area under the SROC curve was 0. 822 5. Conclusion SELDI-TOF-MS is valuable in diagnosis of ovarian cancer.

Objective To investigate the distribution and the characteristics of drug resista-nce of coagulase negative Staphylococcus,and to provide the reference for properly choosi-ng antibiotic therapy of coagulase negative Staphylococcal infecs.Methods Contrasted 484 coagulase negative Staphylococcus(CNS) species isolated in hospital from 2001 to 2003 and 512 species from 2004 to 2007.Nosocomial CNS was identified and then drug resistance test was performed by K-B method,Nitrocefin method was utilized to detect β-lactamase.Clinical and Laboratory Standards Institute.Perfor-mance Standards forantimicro-bial susceptibility testing;Fifteenth information supplement.CLSI/NCCLS document M100-S17[J].Clinical and Laboratory Standards.Results The drug-resistant rate of.CNS to penicillin,erythromycin,oxacillin,amoxicillin/clavulanate,clindamycin,cepzolin,ceftriaxone and cefepime was high(>70%),whereas that to rifampin,nitrofurantoin,amikacin,tetracycline and trimetholvrim/sulfamethoxazole was low(<50%).No drug-resistant CNS strains to vancomyein were found.Conclusions,and no outstanding imparity from two groups.Conclusion The patterfi of resistance has changed greatly in the past 3 years.Clinical physicians should pay attention to properly choosing and using antibiotics.

Objective To investigate.the co-stimulatory pathway of gastric cancer patients by examining the expression of co-stimulatory molecules CD28,CTLA.4(CDl 52)and B7 molecules on peripheral blood lymphocytes(PBLC)in patients with gastric cancer,and to discuss the role of costimulatory pathway in the pathogenesis of gastric cancer.Methods The expression of CD28,CD152,CD3,CD4,CD8 and B7 molecules on peripheral blood lymphocytes and T cell subpopulation were measured by flow cytometry in 56 patients with gastric cancer,and the data was compared and analyzed with that in gastric benign tumor group and healthy control group.Results The expression levels of CD3+,CD4+,CD3+ CD28+,B7-1(CD80),B7-2(CD86)and CD41/CD8+on PBLC in patients with gastric cancer was significantly lower than that of benign stomach tumor group and healthy control group.The expression levels of CD3+ CDl52+ and CD8+ on PBLC in patients with gastric cancer were significantly higher than those of gastric benign tumor group and healthy control group.Conclusion Peripheral lymphocytcs in gastric cancer patients express low level of CD28 and B7 molecules and high level of CDI 52 molecules,which results in dysfunction of B7:CD28/CTLA-4 co-stimulation pathway and in turn affects the capacity of T cell to eliminate tumor cells,which makes tumor cells evading the immune surveillance and metastasizing.

Objective To investigate serum total prostate special antigen(tPSA),complexed prostate special antigen(cPSA)and cPSA/tPSA ratio(C/T) in the differential diagnosis of benign prostate hyperplasia(BPH)and prostate cancer(PCa).Methods A total of 184 serum samples from hospital outpatient and inpatient were collected from May 2009 to December 2015,and which were divided into three groups including other diseases 68 patients,benign prostate hyperplasia(BPH group)80 patients,prostate cancer (PCa group)36 patients.Chemiluminescence immunoassay was used to detect the concentration of tPSA and cPSA,and the C/T ratio were calculated in the study.Results The tPSA,ePSA levels and C/T ratio were significant different from patients with PCa,BPH and other diseases group(P＜0.01).Compared with BPH group(r=0.661),the tPSA and cPSA levels have a higher relativity in PCa group(cPSA=0.708 × tPSA-0.219,r=0.956).While when tPSA level ranges 4.0-10.0 ng/mL),no significant difference of tPSA,cPSA levels and C/T ratio were found in these 3 groups(P＞0.05).Conclusion Combined detection of serum tPSA,cPSA and C/T,making importance in the differential diagnosis of BPH and PCa.

Objective The color of the syphilis quality control material adopted by most detection institutes was the same with the detected serum sample and they were all colorless,transparent or light yellow.There were cases of wrong adding,missing adding or insufficient adding due to the color of quality control materials which was hard to distinguish with naked eyes.To avoid this phenomenon,a new method was established for the distinction of quality control materials.Methods A new method of syphilis quality control materials that had been improved three concentrations control materials:0.125,0.250 and 0.500 NCU/mL.The syphilis diagnostic kit that was created by Shanghai Kehua and Xiamen Yingke was adopted to conduct detection and compare results.Results The difference between stained quality control material and unstained quality control materials had no statistical significance (P＞0.05).Two different reagents were used to detect quality control materials of different concentration for 20 times and the CV were 11.7 ％-13.4％ and 9.3 ％-12.9 ％ respectively.Two different reagents were used to detect quality control materials of different concentration for 30 days and the CV range were 10.1 ％-13.4 ％ and 8.08 ％-12.8 ％.Conclusion Citric yellow staining does not influence the properties of syphilis control materials and it can be used stably for a long time.It is suitable for clinical lab application and promotion.

Objective To master the prevalence of H unan Immunodeficiency Virus (HIV),hepatitis C virus (HCV)and syphilis (RPR)infection and influencing faictors in Liucheng County,Guangxi Province,provided scientific basis for reducing the infection rate among drug users.Methods Analysis of 2011-2015 surveillance data of AIDS sentinel population.Results Male infection is more than female.The ratio of male to female is 1:0.072;The number of male infected with 2011-2015 in each year is 10 times that of female.The affect people between 20 to49 years old was higher than that of others,accounted for 93.66 ％ of the total number of infections.The ntmber of HIV and the number of HCV were downgrade year by year.The number of RPR was at the same level each year.HIV infection between 20 to 29 years old was lower than that of between 30 to 39 and 40 to 49 years old in HIV-infected people.HIV infection between 30 to 39 years old was lower than that of between 4(to 49 years old.The difference was statistically significant(P＜0.01).HCV infection between 20 to 29 years old was lower than that of between 30 to 39 and 40 to 49 and 50 to 59 years old in HCV-infected people.The difference was statistically significant(P＜0.01).RPR infection between 20 to 29 years olt was lower than that of between 30 to 39 and 40 to 49 years old in RPR-infected people.The difference was statistically significant(P＜0.01).Conclusion To strengthen the detection of drug users and behavior intervention,reduce the harm of AIDS,protect the health and safety of the people,is an important way to curb the prevalence of AIDS.

Objective To explore the best cut-off value of serum human epididymis protein 4(HE4)in the diagnosis of ovarian cancer,and analyze the clinical significance of combined detection of HE4,carbohydrate antigen 125 (CA125)and D-dimer for early screening and diagnosis of ovarian cancer.Methods A total of 49 patients with ovarian cancer,63 patients with benign tumor and 47 healthy women were collected.The content of CA125,HE4 and D-dimer were detected by chemical luminescence immunoassay and turbidimetric inhibition immunoassay,respectively.Results The new cut-off value of HE4 was 54.31 pmol/L.The serum levels of HE4,CA125 and D-dimer in the ovarian cancer group were all significant higher than those in the ovarian benign tumor group and the healthy group(P＜0.05).No significant difference between the healthy group and benign disease group was found in CA125 and D-dimer content(P=0.293,0.359),while a significant difference in HE4 level(P＜0.01).The sensitivity and specificity of combined detection of the HE4+CA125 increased to 91.8％,85.4％,which was higher than that of the diagnosis detection of the three indicators respectively.The sensitivity and specificity of combined detection of the HE4 +CA125 +D-dimer were 95.0％,76.5％,which was higher than the combined detection of the HE4+CA125.Conclusion The combined detection of CA125+HE4+D-dimer could improve the sensitivity for early diagnosis and screening of ovarian cancer.

Objective To explore the effect of miRNA-146a on the proliferation and apoptosis of vascular smooth muscle cells and its mechanism.Methods Taking the SDP grade healthy male SD rats as the experimental subjects,After the digestion of vascular smooth muscle cells(VSMC),50 nmol/L transfected miRNA-146a antisense oligonucleotides,missense chain and the same dose of phosphate buffer(PBS)were compared.Results The number and absorbance value of VSMC in the experimental group were lower than those in the other two groups after transfection with 48 h,the apoptosis rate was significantly higher than that of the other two groups,the expression level of nuclear factor κBp65 and PCNA was lower than the other two groups,and the difference was statistically significant(P＜0.05).Conclusion miRNA-146a can promote the proliferation of VSMC,inhibiting the apoptosis of the cells,the mechanism of action is related to the increase of nuclear factor κBp65 and PCNA expression.

Objective To explore the application value of automated cell DNA ploidy analysis system in the diagnosis of benign and malignant serous cavity effusion.Methods 262 cases of serous cavity effusion(169 cases of pleural effusion,78 cases of ascites,15 cases of pericardial effusion)were treated by centrifugation,2 slices of each sample were made.One of them used for dyeing Feulgen,which given automatic cell DNA ploidy analysis,another one for Papanicolaou staining,with a conventional cytology.The positive detection rate of these 2 kinds of different detection methods for malignant serous cavity effusion were compared.Results 119 cases(45.4％)of 262 cases abnormal were detected by conventional cytology of serous cavity effusion.Meanwhile,113 cases (43.1 ％)were detected abnormal by DNA ploidy analysis in the same samples.73 cases of tumor cells and suspicious tumor cells were found by conventional cytology,and different ploidy cells were found in all of these samples In conventional cells,46 cases of nuclear heterogeneous cells were found,while only 34 cases exist different ploidy cells.Conclusion Automated cell DNA ploidy analysis system is helpful to improve the positive diagnosis rate of serous cavity effusion,which can be used as an important auxiliary means of cytology.

Objective To investigate the role of ceramide pathway in cell proliferation and early apoptosis induction in U251 glioma cell after cannabinoid receptora agent anandmide(AEA)treatment.Methods U251 gliom cells were treated with AEA(1-10 μmol/L),Ceramide(5-20 μmol/L) and fumonisin B1 (FB1) (10 μmol/L) pretreatment.The growth inhibition rate of U251 was investigated by MTT assay.The early events of the apoptosis were measured by flow cytometry using annexin-V/propium iodide(PI) double staining method.Results Different concentrations of AEA inhibited the proliferation of human glioma U251 cells,and had synergistic effect with CM by FB1(10 μmol/L)pretreatment for 24 h.After exposure to AEA(10 μmol/L)for 24 h,U251 gliomacells could undergo the early cell apoptosis which was affected by FB1(10 μmol/L).Conclusion AEA through the CM de novo synthesis pathway,and CM concentration was lazy in collaboration,thus inhibiting human glioma U251 cell proliferation and induce early apoptosis.