Objective To study tannins of Corylus heterophylla from Heilongjiang Province. Methods The constituents were separated and purified repeatedly by macroporons resin chromatography methods and their structures were identified by 1H-NMR, 13C-NMR, FAB-MS, and 1H-1HCOSY spectra, and chemical methods as well. Results Three tannins were obtained from C. heterophylla. They were heterophylliin D (Ⅰ), roxbin A ( Ⅱ ), and rugosin F ( Ⅲ ). Conclusion Heterophylliin D ( Ⅰ ) is isolated from the extract of C. heterophylla leaves as a new dimmer tannin firstly.

Objective Based on the activities of antihistamine release to study the compounds from Bidens parvi-flora and find biological active compounds. Methods The chemical constituents from B.parviflora were isolated by silica gel and Sphadex LH-20 column chromatographies and purified by preparative HPLC. The chemical structures had been identified by physiochemical properties and spectroscopic methods. Results Six caffeoylquinic acid derivatives were identified as 3, 5-di-O-caffeoylquinic acid ( Ⅰ ),3, 4-di-O-caffeoylquinic acid ( Ⅱ ), 4, 5-di-O-caffeoylquinic acid ( Ⅲ ), 4-O-caffeoylquinic acid ( Ⅳ ), 5-O-caffeoylquinic acid ( Ⅴ ), 4-[3-(3, 4-dihydroxy-phenyl)-acryloyloxy]-2, 3-dihydroxy-2-methyl-butyric acid ( Ⅵ ). Conclusion Compounds Ⅰ - Ⅵ are first obtained from B. parviflora and Ⅵ is new one. Some of the compounds exhibit the activities in antiallergic assays. Moreover, the structure-activity relationships of these compounds have been also discussed in this paper.

Objective To study the antitumor natural products from Berlandiera lyatra. Methods Compounds were isolated by liquid/liquid partition and chromatography on Sephadex LH20 and silica gel columns. All process of the fractionation were run with the guidance of cytotoxicity bioassay by MTT method against three human cancer cell lines. The chemical structures of bio-active compounds were identified on the basis of spectroscopic data. Results From the CHCl3-soluable fraction of the title plant, two cytotoxic compounds were isolated. They were determined as 3α-epoxypumilin and pumilin. Conclusion 3α-epoxypumilin and pumilin were responsible major compounds for the cytotoxicity in the methanolic extract of the plant. Both compounds were isolated from the B. lyatra and were reported to be of antitumor activity for the first time.

Objective To study the chemical constituents from the stems of Dendrobium nobile. Methods Compounds were isolated through various chromatographic techniques and identified by spectral data. Results Twelve phenolic compounds were obtained. Their structures were characterized as dihydroconiferyl dihydro-p-coumarate (Ⅰ), vanillin (Ⅱ), apocynin (Ⅲ), p-hydroxybenzaldehyde (Ⅳ), syringaldehyde (Ⅴ), syringic acid (Ⅵ), syringylethanone (Ⅶ), α-hydroxypropiosyringone (Ⅷ), coniferyl aldehyde (Ⅸ), dihydroconiferyl alcohol (Ⅹ), 2-hydroxyphenylpropanol (Ⅺ), and 3-hydroxy-4-methoxyphenylethanol (Ⅻ), respectively. Conclusion All above compounds are reported from this plant for the first time. Compounds Ⅰ and Ⅲ -Ⅻ are reported for the first time from the plants of Dendrobium Sw.

Objective To study the triterpenoid saponin from bulbs of Bolbostemma paniculatum.Methods The compound was isolated by repeated silica gel chromatographies and its strcuture was eluci-dated on the basis of physico chemical property and spectral analysis. Results A novel triterpenoidsaponin was isolated and determined as olean 12-en-28-oic acid, 3- {[2-O-[6-O-[(3R)-4-carboxy-3-hydrox-y-3-methyl-1-oxobutyl]-β-D-glucopyranosyl]-β-D-glucopyranosyl] oxy}-2, 16, 23-trihydroxy-28-[2-O-α-L-rhamnose (1→2)-α-L-arabinopyranosyl] ester ( I φ. Conclusion Compound I is a novel compoundnamed as dexylosyltubeimoside Ⅲ.

Object To investigate the chemical constituents from the roots of Glycyrrhiza uralensis Fisch. Methods The constituents were isolated on normal and reversed silica gel column chromatography and their structures were identified by spectral evidence. Results A new oleanane-type triterpenoid saponin and twelve known compounds, including two triterpenoid saponins, two cumarins and eight flavonoids, were isolated. Conclusion The new compound was elucidated as 3-O-[β-D-(6-methyl) glucuronopyranosyl (1→2)-D-glucuronopyranosyl]-24-hydroxy-glabrolide on the basis of ESI-MS, 1HNMR,13CNMR, HMQC and HMBC spectral evidence.

Object To explore the antitumor mechanism of Stellera chamaejasme Linn.(SC).Methods SC containing-serum(SCCS)was derived from mice pretreated with different doses of SC.Cultured human leukemia HL-60 and human gastric adenocarcinoma SGC-7901 cells were used.Inhibition of proliferation was measured using MTT assay.Morphological assessment of apoptosis was performed with fluorescence microscope.DNA fragmentation was assessed by agarose gel electrophoresis and flow cytometry.Expression of bcl-2 protein was measured with immunohistochemistry.Results Exposure of exponentially growing HL-60 cells to mice serum containing 10% SC(pretreated with SC3,6, and 12 g/kg)for 48h resulted in growth inhibition in a dose-dependent manner.Typical morphological changes of apoptosis and DNA fragmentation in HL-60 cells were induced."Apobodies'in the apoptotic cells were observed,'ladder"pattern of agarose gel electrophoresis of DNA from 11.7% to 57.4%.Treatment with SC containing serum decreased the percentage of SGC-7901 cell of bcl-2 protein positive expression from 78.3% to 32.9%.Conclusion SC could induce apoptosis of HL-60 cells and decrease the expression of bcl-2 protein of gastric adenocarcinoma SGC-7901 cells.

Cordyceps sinensis (Berk.) Sacc.; anti-tumor activity; active component; mechanism

To probe tentatively into the relations of Terminalia chebula Retz., Terminalia billerica (Gaertn.) Roxb, and Phyllanthus emblica L. between TCM and Tibetan medicine. Methods　Textual researches of materia medica were carried out and their history in clinical application were traced. Results　T. chebula, T. billerica, P. emblica were all of foreign origin and were introduced into China with Buddhism. As compared in medical practice, the Tibetan autonomony region has their particular choice and preference. Conclusion　We should apply modern scientific research achievements to carry out deep mutual exchanges to enrich TCM.

Object　To provide a scientific basis for the identification and rational clinical use of the two herbal drugs. Methods　Experiments were carried out by comparing their properties, microscopic characteristics, UV spectra, TLC and GC chromatograms. Results　There are remarkable differences between the two crude drugs in these respects, and a convenient and effective method for their differentiation and identification has been established. Conclusion　Ligusticum sinense Hort. and Ligusticum sinense Oliv. cv. chaxiong Mass should not be used one instead of the other.