Utilizing the classical method——destruction test of thermostatic heat acceleration and using the residual content of total anthraquinone of Radix et Rhizoma Rhei as an index, the shelf life of Maren Soft Capsules was predicted as 2.13 years when the data were computed and dealt according to Arrhenius eguation

AIM: To investigate the epidemiological characteristic and causative factors of anaphylactic shock caused by Shuanghuanglian Injection(Flos Lonicerae Japonicae,Radix Scutellariae,Fructus forsythiae). METHODS: Domestic medicine periodicals were searched between 1978 and 2005,and 72 cases with anaphylactic shock caused by Shuanghuanglian Injection were analysed by literature metrologic method. RESULTS: There were large proportion of young sufferers from age 18 to 44 in the case reports,and male 52.11%,female 47.89%,the primary affections were mainly upper respiratory tract infection,the occurrence of ADRs was commonly within 30 mins after injection,seven patients died of the terrible anaphylactic shock. CONCLUSION: Shuanghuanglian Injection may induce dangerous,even fatal anaphylactic shock,its mechanism includes many factors,such as patient's physical constitution.

AIM:To study release rate in-vitro of the panax notoginoside micro-porous osmotic pump(MPOP) tablets.METHODS:To investigate the effects of different dissolution methods,paddle stirring rate and dissolved mediums on the release rate in-vitro of the panax notoginoside MPOP tablets.RESULTS:The delivery rate was not influenced by the dissolution methods,paddle stirring rate and when the pH of dissolution medium was between(3.5)-7.6,the delivery rate was not influenced,too.However,when the pH of dissolved medium was at 1.0,the release rate was decreased severely.CONCLUSION:Panax notoginoside MPOP tablets have a stable release curve except for in the condition of the acid dissolved medium.

AIM: To explore the machanism of calcined mica by comparing the difference in crystal structure and inorganic elements of raw mica and calcined ones. METHODS: Crystal structures were compared by IR spectrum and X-ray crystallography and the contents of element in the prevention were determined with ICP-AES. RESULTS: After being calcined,crystal phase transformation changed in gold mica and did not change in white mica.Both of their chemical composition changed,crystal water decreased and crystal defects increased.There were obvious differences in the contents of inorganic elements.The content in white mica was obviously more than that in gold mica.After being calcined,the value of K/Na in white mica decreased and the contents of Ca,Mg increased,while the content of Mg increased and the content of Ca decreased in gold mica. CONCLUSION: There are(actually) many differences in contents of elements and chemical structure in white and gold mica before and after being calcined,which may explain the mechanism of calcined mica.

AIM:To establish a HPLC method for determining simultaneously gentiopicroside and geniposide in Longdan Xiegan Pill(Radix et rhizoma Gentianae,Fructus Gardeniae,Radix Scutellariae,etc.).METHODS:C_ 18 column(5 ?m,4.6 mm?250 mm)was used.The mobile phase was methanol-water(23∶77).The detecting wavelength and the temperature of column were at 238 nm and at 40 ℃ respectively.RESULTS:The linear detection range of gentiopicroside was 3.03-30.30 ?g/mL(r=1.000 0),and the average recovery was 98.43%(RSD=1.08%).The linear detection range of geniposide was 9.76-97.60 ?g/mL(r=0.999 9),and the average recovery was 99.95%(RSD=0.91%).CONCLUSION:Two ingredients in Longdan Xiegan Pill could be determined simultaneously by this method which is convenient,rapid and accurate.It can be used for quality control in the production of Longdan Xiegan Pill.

AIM:To establish a method for GC fingerprint determination of the chemical constituents in the volatile oil of Eucalyptus globulus Labill.in Yunnan Province,China. METHODS: GC was used to optimize the fingerprint determination method,and to identify the main peaks in the GC fingerprint. RESULTS: 16 mutual peaks were gained on the base of 10 batches of samples. CONCLUSION: Stable and reproducible GC fingerprint is helpful to further analyze the volatile oil constituent of Eucalyptus globulus Labill.

AIM:To study the feasibility of online monitoring and controlling of the key process of column chromatographic separation in the production of Salvianolate by combining near-infrared(NIR) spectrum and HPLC fingerprinting. METHODS: Partial Least Square(PLS) regression was used to model the correlation of NIR spectra with the concentrations of salvianolic acid B,and the influences of light path,wavelength selection,and preprocessing method on the PLS model were investigated systematically. RESULTS: A 2 mm light path was better than 1 mm one,and with 2 mm light path,the optimal wave-number was in the range of 6 102.1-5 446.3 cm~(-1) and the optimal preprocessing method was the vector normalization.The root mean-square error of PLS model on test samples was 0.234 mg/mL,and R~2 was 0.995 2. CONCLUSION: This method is proved to be fast,convenient,and precise.It can be used to online monitoring and quality control of the manufacturing of Salvianolate.

AIM:The polysaccharides of Hedyotis difusa Willd of different sources were extracted and its content and composition of the polysaccharide was analyzed. METHODS: The microwave was employed for the aqueous extract of Hedyotis difusa Willd and the content of total sugar was determined by phenol-sulfur test,the monosaccharides composition was determined by HPLC with pre-column derivatization. RESULTS: The polysaccharide obtained from the samples of Guangdong Province had the highest content.The analytic data showed that the composition of monosaccharides in hydrolytic polysaccharides of Hedyotis difusa Willd were mannose,glucose,galactose,arabinose and rhamnose,the average content of polysaccharides was 9.82%. CONCLUSION: The HPLC method with pre-column derivatization is appropriate for the analysis of monosaccharide composition of Hedyotis difusa Willd polysaccharides and the method is simple,quick and accurate.

AIM:To determine the triterpenoids content from Antrodia camphorata in submerged culture. METHODS: Oleanolic acid as the standard,5% vanillin-acetic acid and perchloric acid as the developer,the spectrophotometric method was used for determining the triterpenoids content from Antrodia camphorata in submerged culture. RESULTS: The calibration curve was linear(R~2=0.997 8) in the range of 20～140 ?g, and the regression equation was Y=0.004 9X-0.018 4.The content of Antrodia camphorata in submerged culture was(about) 1.73%. CONCLUSION: The spectrophotometry is suitable for the determination of total triterpenoids from Antrodia camphorata in submerged culture.It is convenient to operate and the result is stable and reproducible.

AIM:To study the mechanism of foam floatation in the processing of Indigo Raturalis,and provide theory to support the industrialization of Indigo Raturalis processing by foam fioatation technique. METHODS: To study the critical problems of the processing of Indigo Raturalis by foam floatation technique: the change of particle size distribution after the processing of Indigo Raturalis;The surface activity of frothing materials in Indigo Raturalis;the distinction of the foam to absorb the Indigo particles and other particles(CaCO_3);the absorbability of the Indigo particles to absorb different surface active agents. RESULTS: 1.The Indigo particles were easily absorbed by the foam,but others(CaCO_3) were not;2.There were some frothing materials with good frothing capability in the alkaline system of Indigo Raturalis,3 In the alkaline system,the Indigo particles could be absorbed by the foam of strong acid surface active agents. CONCLUSION: In the alkaline system of Indigo Raturalis,with itself surface active agents,it is effective to process Indigo Raturalis by foam floatation technique.