Objective To establish a experimental method for evaluating nerve regeneration after operation by means of tracing the retrograde axonal plasm flow by 125 I HRP Methods Eighteen SD rats were used The right sciatic nerve of mouse was transected at the site 0 8cm distal to sciatic notch and then anastomosed immediately Four weeks later,500?l of 125 I HRP solution was injected into the right musculi triceps surae The right sciatic nerve segment proximal to the anastomosis site was cut for checking up the intensity of radioactivity in each tissue at 24 hours after injection The fiber number of nerve distal to the site of anastomosis were calculated The relationship between the intensity of radioactivity and the fiber number was analysed Results The relationship between the intensity of radioactivity of sciatic nerve segment proximal to the site of anastomosis and the fiber number of distal sciatic nerve segment was correlation and there was statistic significance. Conclusion The status of nerve regeneration distal to the site of anastomosis can be evaluated by means of intensity of 125 Iodine in the nerve segment proximal to the site of anastomosis

Objective To study the retrograde axonal transport of Schwann cell derived neurotrophic factor(SDNF)by motor neurons Methods SDNF was labeled with Chloramine T, 125 I SDNF and 100 fold excess of unlabelled SDNF was injected into the right hindlegs of neonatal rats separately The spinal cords was removed for counting in a  counter Results The radioactive level of experimental side was more than contralateral side The transport was blocked by 100 fold excess of unlabelled SDNF Conclusion SDNF was transported retrogradely by spinal motor neurons

Objectives To investigate the process and the result of the repair of the sciatic nerve in rat by chitosan tube and polyglycolic fiber Methods The left sciatic nerves of rats were transected with 10 mm gap, then repaired with the nerve grafts in combination of NRF Walking track analysis, immunohistochemical and transmission electron microscopic examinations were carried out at different time after the operation Results Fibrous membrane and capillary could be observed on the surface of the grafts at the first week after the operation; at the 4th week, the regenerated nerve fibers grew more than half of the distances of the gap; at the 8th week, regenerated nerve fibers overcame the gap, many myelinated nerve fibers and thick myelin sheathes could be observed; at the 16th week, the grafts almost degraded and were substituted by the regenerated nerve Walking track analysis showed that sciatic nerve function recovery appeared at the 8th week, and that at the 16th week, approximately 60% of the sciatic nerve function was recovered Conclusion The nerve graft with NRF can stimulate the growth of vessels and the formation of the fibrous membrane, and is useful to the migration of Schwann cells and regeneration of axons, and degrade after the nerve regeneration

Objective To analyse the molecular structure of Schwann cell derived neurotrophic protein (SDNP) by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI TOF MS) Methods The purity of SDNP was digested byTPCK trypsin and detected by MALDI TOF MS, mass mapping was determined and partial sequences of the protein have neen analyzed by the mass data of fragment ion peaks in the fragmentation analysis and structural TOF MS (FAST) spectra The primary partial structure of SDNP was identified by searching the protein databases Results The integrity SDNP in peak detected by MALDI TOF MS has 66?10 3 MW and higher purity, because no other peaks exists except double charge peak The mass mapping of many peptides was determined and 8 peptides of them have been retrieved in MS Fit database, there is not the same protein in database Hypothetical protein has 5 peptides homology with the sample (62%) FAST spectra of 2305 Da shows the primary partial structure of SDNP after searching the protein MS Tag database Conclusions The molecular weight of SDNP detected by MALDI TOF MS is 66?10 3, The sequence of partial amino acid is EPVKKVTNSRRAKRTKPNGHIAN

Objective To discuss microsurgical experience for transsuboccipital retrosigmoid sinus approach of large acoustic neuronomas,and improve the rate of tumor removing,and facial nerve and auditory nerve preserving Methods A retrospective analysis of 39 patients with acoustic neuronomas which were more than 4 cm in diameter were treated by microsurgery between January 1994 and December 2000 Tumor's excision was performed by transsuboccipital retrosigmoid sinus approach The surgical main points of transsuboccipital retrosigmoid sinus approach,and their experience of total removal,and rate of facial nerve and auditory nerve preservation were described Results The tumors were totally removed microsurgically in 34 patients,the total removal rate was 87 2%,and subtotal removal in 5 patients Facial nerve was anatomically preserved in 31 cases,the rate of facial nerve preservation was 79 5%,but the function of facial nerve was recovered in 22 cases (56 4%) The auditory nerve was preserved anatomically in 15 cases (38 5%) and functionally in 7 cases (17 95%) Conclusions An minimally invasive microsurgical treatment are crucial for increasing a satisfactory living quality of the patients It is considered that the total removal rate of tumor,the preservative rate of facial nerve and auditory depend upon microsurgical technique and intraoperative facial nerve monitoring in acoustic neuromas surgery

Objective Evaluation of application of looping vascushunt tubes in microsurgical total removal of para sinus meningiomas Methods Eighteen patients harboring para sinus meningiomas were treated in our hospital from Feb,2000 to Mar,2002,Tumors were located in the posterior two thirds superior sagittal sinus in 17 patients and in the congenitally isolated transverse sinus in one patient Looping vascushunt were applied in all cases and tumors were removed under microscope using microsurgical techniques Results Tumors and involved sinus walls were resected (Simpson grade Ⅰ) in all patients Venous sinus reconstruction were achieved in 12 patients using artificial blood vessels,sinus walls were repaired with duramater in 4 patient, venous sinus ligation was applied in two patients because of the sufficent collateral venous drainage All patients recovered and no mortality occurred Conclusions Application of vascushunt tubes is one of the important techniques in microsurgical total resection of para sinus meningiomas

Objective To investigate the effect of end to side arterial anastomosis in the transplantations of free tissue flap Methods The end to side arterial anastomosis were used in 47 free flaps of 46 cases in this series The arterial stomas were respectively prepared into the cross section (13/47),the inclined plane angled at 45 degree (26/47) and fish mouth shaped plane (8/47) The interrupture anastomosis and continuous anastomosis following two fixed point sutures,and the continuous anastomosis following four fixed point sutures were respectively used in 28,10 and 9 arterial anastomotic stomas Results There were not postoperative embolism in all end to side anastomotic stomas in this series During the operations the embolisms of the cutaneous branch caused by a detached mini thrombus from the anastomotic stomas were occurred in 2 cases,and the arterial crisis were eliminated by reversely milking out the detached mini thrombus All flaps survived except one due to venous embolism Conclusions It is a effective and safe method that the end to side arterial anastomosis are used in the transplantation of free flap

Objective To analysis and evaluate the long term results after endoscopic lumbar discectomy, which was performed through a minor incision with guidance of TV screen Methods Forty-one operations were followed up, to evaluate their postoperative conditions according to Nakano scale, analysis the causes of repeated operations, and also compared them with other lumbar discectomy Results All symptoms and disabilities disappeared, and they return to work 3 months after treatments Three of them relapsed respectively in 12,16 and 52 months But after receiving regular treatment of open window on vertebral lamina, they all were recovered Conclusion Minimal invasive videoscopic lumbar discectomy led to less trauma, earlier recovery, lower costs and less influence on biodynamic structure of spine Its early and long term results are satisfactory So it is concerned to a new choice of operative methods

Objective To evaluate the clinical effect of neuromatosis scoliosis treated by vascularized grafted rib Method The clinical date of 16 cases of neuromatosis scoliosis treated by surgical operation were analysed retrospectively Result Sixteen patients with scolisis caused by neurofibromatosis were treated by this technique The clinical result was satisfactory and solid fusion was observed in all patients within 6 months after operation Conclusion This operation is an effective method for the treatment of neuromatosis scoliosis

Objective To investigate the clinical result of reconstructed thumbs and fingers using frozen allogeneic composite tissues of fingers and autogenous toe Methods Fifty thumbs and fingers were reconstructed in 24 patients (25 hands) by allogeneic composite tissues of finger wrappoed by free neurovascular big toe nail skin flap and combined transplantation with autogenous second toe 15 patients were followed up for 4 years in average with examination in the ambulant clinic, communication and X ray photography to evaluate the clinical effect Results Recovered sensory function of the reconstructed fingers as well as good opposition function were seen There were Charcot's like arthrosis of allogeneic MP and arthroclisisof IP in 2 cases The postoperative gait of the doner's feet was normal The site of grafted skin on big toe was rubbed easily in 4 cases and the valgus hallexo of the doner's foot were seen in 3 cases, the plantaris callus in 2 cases Postoperative immunological rejection didn't elicit The junction between implanted allo phalangen tendon and autophalanges tendon were reliably healed Conclusions This operation gives reconstructed more fingers without sacrificing more toes for patients The walking function of donor foot is good The antigenicity of allogeneic grafts could be abated after preservation under 30℃ or frozen dried