Objective To explore the reconstruction effects of lower hemifascial scar by cervical skin excessive volume expansion. Methods Single 100 ml or 150 ml standard volume tissue expander was implanted under the cervical skin in 50 patients with lower hemifascial scar. After the tissue expanders were injected with 4-5 times than the designed volume, the scar was resurfaced with the expanded flap. Results The excessively expanded cervical flaps of 50 patients all were successfully used to reconstruct the facial scar. The expanded flap was matched well with surrounding skin after 3-12 months following-up. No obvious contracture was observed. The district recontrueted by expended flaps have the normal expression aetivities. Conclusion Excessive expansion of cervical skin can promote the efficiency in the reconstruction of the lower hemifascial scar.

Objective To observe the effect of bcl-2 antisense oligodexyonucleotide (ASODN) by phosphorothiote-modification on the expression of bcl-2 mRNA and protein in human melanoma A375 cells line. Methods With the phosphorothiote-modification and liposome-encapsulation of ASODN, A375 cells were divided into ASODN group, nonsense oligodexyonucleotide (SODN) group and control group. Bcl-2 mRNA and protein were detected by RT-PCR and immunohistochemistry, respectively. Results The expression of bcl-2 protein was remarkably decreased in ASODN group than that in SODN group and control group (53.14 ±4.26 vs 138.22 ± 8.45, 53.14 ± 4.26 vs 141.08 ± 7.83, both P ＜ 0.01 ). The level of bcl-2 mRNA was significantly lower than that in SODN group and control group (0.38 ± 0. 11 vs 0.96 ±0.13, 0. 38 ± 0.11 vs 0. 97 ± 0. 14, both P ＜ 0. 01 ). Conclusion Bcl-2 antisense oligodexyonucleotide could down-regulate the bcl-2 level and block its protein expression.

Objective To evaluate the value of Materialise's interactive medical image control system (MIMICS) in the computer aided surgery, and to offer a reliable system for precise surgical planning and good effect of orthognathic surgery. Methods Based on CT images, geometric characteristics of 2 patients' head were collected for a 3D reconstruction of the human head. Skull, skin, and submucous tissue were reconstructed and optimized separately by MIMICS 10.01. Surgeries were simulated and the soft tissue deformation was predicted according to the surgical planning of experienced surgeons. Results Mesh of skull, skin and submucous tissue were constructed manually for 2 patients. Surgical procedures were simulated and the prediction of soft tissue deformation was realized by MIMICS, which could be visualized in any angle. Conclusion MIMICS is a helpful digital tool for computer aided surgery and the prediction of soft tissue deformation. MIMICS is also a 3D visualization system for orthognathic surgeries, by which surgeons could realize the surgical planning, revising,and the patient-doctor communication on the computer screen.

Objective To develop new repairing techniques for acquired inferior palpebral region defects. Methods Expanded forehead flaps were used to reconstruct the inferior palpebral defects or post-excision wound surface and the flaps were pedicled with supra-trochlea vessels or ramus frontalis arteriae temporalis superficialis. As for supra-trochlea vessels, contralateral ones were prior to the homolateral ones. The incision site located in the scalp and the major axis of the expander parallel to the forehead. Firstly, the leisions were cut and the subcutaneous tissues loosed to regain the anatomy position of inferior palpebra. Secondly, the expanded flaps were transfered onto the defects by the wound sizes with the supra-trochlea vessels as their pedicles. At last, the pedicles were cut 3 weeks later.For ramus frontalis arteriae temporalis superficialis, the flap was transfered with a subdermal pedicle and the donor site was closed directly. Results There were 10 cases in the present group, 6 for supratrochlea vessels and the 4 others for ramus frontalis arteriae temporalis superficialis. All the flaps survived successfully. 3 cases returned with optimistic outcomes 6 months later. Conclusion The expanded forehead flaps are fit for repairing the inferior palpebral defects, which can successfully avoid ectropion. This technique is very useful for reconstructing the texture of the site of defects.

Objective To study arterial distribution of rectus abdominis musculocutaneous flap and to evaluate whether it can be divided into several units for reconstruction. Methods The arteries of the rectus abdominis musculocutaneous flap were studied on 60 sides of cadavers by dissection and angiography. Results The superior epigastric artery (SEA) and the inferior epigastric artery (IEA) continued in a longitudinal direction. Most of their branches took on a typical spiral configuration and communicated with each other within muscle above the level of umbilicus. Many perforating arteries penetrated through the anterior rectus sheath to get to the overlying skin, but the highest concentration of major perforators were in the paraumbilical area. The inferior epigastric artery was more significant than superior epigastric artery in supplying the skin of the musculocutaneous flap. Based on thefstudies of the vascular anatomy of muscles, we could classify arterial distribution into 3 types: type Ⅰ (SEA 26.5 %, IEA 34.6%) revealed a single main intramuscular artery: type Ⅱ (SEA 64.7 %, IEA 48.1%) had two major intramuscular branches; type Ⅲ (SEA 8.8%, IEA 17.3 %) revealed three intramuscular branches. Our anatomic studies showed that the superior and inferior epigastric artery bifurcated or divided into more than two main branches in the majority of cases (SEA 73.5%, IEA 65.4%). Conclusion The rectus abdominis musculocutaneous flap could often be divided into several regions for breast construction which is based on the distribution of each branch of the artery.

Objective To investigate the changes of the constitution and its ratio of collagen fiber in the process of masseter reattachment following different osteotomies of the prominent mandibular angle so as to offer guidance for the resection of mandibular angle. Methods Sixteen adult goats were randomized into four groups. In group A we performed unilateral curved osteotomy of the mandibular angle. In group B unilateral curved ostectomy was performed with partial masseter resection. In group C unilateral angle splitting ostectomy, while in group D unilateral dissection of the masseter muscle was conducted. The constitution and its ratio of collagen fiber in the interface were observed at 1-month, 2-month, 3-month, and 6-month after operation. Results On the changes of collagen fiber in the process of muscular reattachment, at 1-month post-operation, the constitution of collagen fiber (types Ⅰ and Ⅲ) in groups A and B were significantly different from that of control group (P<0.05). However, both groups C and D had no statistic difference from control group (P>0.05). At 2-month, 3-month and 6-month post-operation, those of all experimental groups had no statistic difference from control group. And with time, the percentage of collagen fiber type Ⅰ increased and type Ⅲ decreased gradually. Conclusion The recovery sequences of masseter muscle reattachment in this study are firstly group C, secondly group A and finally group B. It suggests that the recoveries of mastication and other oral activities are different. Group B turns out to be with a slow muscle reattachment. Thus, we recommend treating different kinds of mandibular hypertrophy with different ostectomies.

Objective To investigate the clinical results of immediate loading of a single anterior implant. Methods 26 implants were placed into anterior sites. Provisional prosthesis were placed at the time of fixture placement. It did not occlude in any pathway. Restoration was for esthetic only, not for function. Results No fixture was lost since the immediate restoration was delivered, the minimum being 12 months and the maximum 72 months. Conclusion Clinical studies on the placement of immediate restoration in anterior implant of maxilla have revealed predictable results.

Objective To investigate the relevant data and aesthetic standards of hip and thigh profile in young females from the central plain area of China, aiming to provide a credible reference for the clinical application. Methods Measurements were made in 551 young healthy females aged 16 to 21 years, including waist circumference, hip circumference, maximum thigh circumference, thigh length and the ratio between thigh length and height. Based on these data, the features of the hip and thigh profile were analyzed. Results Waist circumference, hip circumference, maximum thigh circumference, thigh length and the ratio between thigh length and height were ( 70. 74 ± 5.86 ), ( 87.16 ± 4. 19 ), ( 51.87 ± 3.27 ),(42.35 ±2.38) cm and (26. 1 ± 1.23) %, respectively. The hip and maximum thigh circumference showed significant difference between different height and weight groups. The thigh length and the ratio between thigh length and height were positively correlated with height. Conclusion The parameters of hip and thigh profile in young females from the central plain area are of significant values in providing quantitative references for the plastic surgery and evaluation of the operation,especially in liposuction.

Objective To study the cellular affinity of the inorganic active element bone scaffold materials. Methods The specific surface area, pore size distribution, porous ratio, permeability ratio of the inorganic active element bone scaffold materials were evaluated in vitro. Human marrow mesenchymal stem cells(MSCs) were cultured and identified by the flow cytometry, the third passage of the cells were used to culture with scaffold materials, to compare with the cells cultured with pure fetal calf serum as the control group. MTT assay was used to examine the effects of scaffold materials on MSCs viability, the growth of cells was observed by scanning electron microscope (SEM). Results The specific surface area of the inorganic active element bone scaffold materials was 210 m2/g, mean pore size was 6 nm, porous ratio was 90 %, and permeability ratio was34 %. MTT assay showed significant difference in the number of cells between the scaffold materials and the control group (P ＜ 0.05 ) on the seventh day, MSCs co-cultured with scaffold materials exhibited good growth as observed under SEM. Conclusion The inorganic active element bone scaffold materials possess preferable cellular affinity, which could be an overall-developed and high potential scaffold material of bone tissue engineering.

Objective To evaluate the safety and efficacy of polymethylmethacrylate/PMMA (Artecoll) as an injectable dermal filler after using laser, radiofrequency and intensive pulse light. Methods In vitro, factional photothermolysis (Affirm) was directly used on the PMMA to see if there was any change in the structure or surface smoothness. In vivo, pig and guinea pig were used as the short-term and long-term models, respectively, for Artecoll. The dermal filler was first injected and then laser treatments conducted.The skin sample was observed through light microscope, scanning electronic microscope and transmission electronic microscope to see whether there were any structural changes, infiltration of inflammatory cells and the regeneration of collagen. Results Through the microscopic observation of in vitro experiment and the animal models, no distortion or rupture of the microsphere were found after the laser treatment. No uneven surface was found in the PMMA microsphere either. The microshpere was surrounded by the inflammatory cells. The infiltration was mild to intermediate with few foreign body giant cells even after several treatments of laser. There was no macrophage seen. But the collagen regeneration was distinguishable after the laser treatment. No obvious structural change was found eventually. Conclusion Artecoll is a safe and effective permanent injectable dermal filler for wrinkle reduction and contour refining.