Objective To explore MSCT optimal examination time window for patients with occult rib fracture, and provide objective evidence for forensic injury. Methods Totally 105 cases with chest trauma were retrospectively analysed. They were examined in the first week after trauma and re-examined in different time windows by MSCT. The quantities of occult rib fractures in the first examination were compared to those in re-examinations. Results The quantities of occult rib fractures at different inspection time windows were mostly different. There was no statistically significant between the quantities of rib fractures in the first week and the second week, the first week and the sixth week later, the fourth week and the fifth to sixth weeks (P>0.05). There was statistically significant between the quantities of rib fractures in the first week and the thrid to sixth weeks, the second week and the third to sixth weeks, the third week and the fourth to sixth weeks (P<0.05). Conclusion The result of the study imply that the fourth to sixth week is the optimal time window of MSCT examination in the occult rib fracture.

Objective To study the toxicokinetics of Cymermethrin and its metabolites in dog bile and provide evidence for forensic cases of identification of Cymermethrin poisoning. Methods 1/4LD50 doses of Cymermethrin were given to 6 male dogs by oral perfusion after the gallbladder fistula surgery on them,and their bile were collected at different time, in which Cymermethrin and its metabolites were extracted by Liquid-liquid extraction with dichloromethane and detected by HPLC-MS-MS. The qualitative analysis was based on retention time and MRM ions. The quantitative analysis was based on an internal standard method and calibration curve. Toxicokinetics equations of Cymermethrin and its metabolites in the bile were established from the c-t curves which were fitted by the WinNonlin toxicokinetics software meanwhie toxicokinetics parameters were obtained. Results The toxicokinetics of Cymermethrin met first-order dynamic equation. The Tmax of Cymermethrin(CYM), 3-phenoxybenzoic acid (3-PBA), 3-(2,2-Dichloroethenyl)-2,2-dimethyl-cyclopropanecarboxylate (DCVA) respectively were 1.52±0.30,1.29±0.04,0.93±0.41 h ; The Cmax of CYM, 3-PBA, DCVA respectively were 0.38±0.03,7.9±1.32,30.9±16.24 μg/mL ; The T1/2 of CYM, 3-PBA, DCVA respectively were3.93±0.71,1.36±0.11,4.49±2.81 h; Conclusion The toxicokinetics of Cymermethrin in dog bile met first-order dynamic equation ; The toxicokinetics model and parameters of Cymermethrin can provide evidence for forensic identification of Cymermethrin poisoning cases.

Objective The content of urea and amino acids in sweat fingerprints is examined by ultraviolet spectrophotometry at ambient temperature of 25 and relative humidity of 65%. Methods In the fingerprint of sweat, urea and amino acids are chemically reacted to produce blue and blue purple matter.Their concentrations are calculated by ultraviolet spectrophotometerand the two ratios are calculated to infer the remaining time. Results Left time of sweat fingerprints shows a linear correlation with urea and amino acid ratio, the linear regression equation is Y=-3.227X+6.706, the female is Y=-3.672X+6.546, the regression equation is Y=-3.276X+6.638.Conclusion The remaining time of human sweat fingerprints is linearly related to the ratio of urea to amino acidsand the regression relation can be used to infer the time of fingerprint retention.

Objective In order to study 275 reported height-associated single nucleotide polymorphisms (SNPs) in males of Northern Han Chinese. Methods Genotype information was detected by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) in 266 Northern Han Chinese male samples. Using PLINK 1.09 and SPSS 19.0 software, three analysis methods were choosen, which were linear regression analysis, logistic regression analysis (tall ≥177.5cm, short ≤167.5cm), and logistic analysis (tall ≥180cm, short ≤165cm). Results We identified 16 SNPs significantly associated with height in our study group(P<0.05), which were analysised by linear regression analysis and logistic regression analysis. Of these, one had been previously described in East Asian (rs11170624), and six had been reported in European (rs10037512、rs17346452、rs2629046、rs7689420、rs8052560、rs7909670). The result of mean variance analysis suggested that the effect of rs11170624、rs17346452、rs7689420 and rs7909670 on height was consistent with previous study. However, compare with European population, the other three loci showed differential contribution of height variation in Northern Han Chinese. Conclusion Further statistical tests in larger numbers of individuals will be required to identify higher population specificity loci affecting stature of Han Chinese, and to build a height prediction model.

Lin Ji is recognized as the founder of modern forensic science in Chinese. He founded the first forensic science,as the first director Professor of Peking Medical Institute of forensic medicine, He founded the first forensic science classroom beiping medical school as the first director Professor, establish the judicial administration department as the first director of the institute of forensic medicine, founded the first magazine in the history of our country "the monthly journal of forensic", founded Central University School of medicine, forensic science and served as professor and director;He introduced modern forensic science, planning to establish six national forensic science classrooms in Chongqing, Peking, Wuhan, Shanghai, Guangzhou, Fengtian;He learned to cultivate the forensic examiner, forensic physician with modern forensic, make the Chinese forensic medicine moved in the process of modernization;His development concept of big Forensic science, view of the experiment Forensic science,the way to make Forensic identification documents disclosed,the professional dedication, personal character and the understanding of forensic medicine have left deep imprint on the history of the development of modern forensic science in our country, which have a far-reaching influence in the past,and in the currently.This is the academic thoughts and contemporary value of Lin Ji.

Alcohol exposure Often observed in the process of traumatic brain injury (TBI), which is closely related to the death of traumatic brain injury. At home and abroad this area research is complex. This paper summarizes the relevant domestic and foreign experimental studies in recent ten years, from the clinical experimental study, animal model application, brain damage identification and other aspects of different serum alcohol levels (BAC) on the impact of brain injury related research progress. This review provides a reference for further research at first, it also has reference for the identification of related deaths.

Objective From the perspective of making full use of database comparison function, giving certain guidelines to analyze mixed DNA profile,compare database,screen comparison results. Methods Using CPI to describe the identification of mixed DNA profile.Using CPBI to estimate reliability of individual samples being included. Results When CPI is less than 10-7, mixed DNA Profile is worth to be compared in database.When the number of alleles at one locus is more than 2, retain an additional allele will not reduce identification too much. According to the CPBI of the included samples,we can find the most reliable sample.

Objective To explore the clinical application value of three-dimensional reconstruction of maxillary canine pulp volume /tooth volume (PV/TV)in individual gender determination.Methods There were 103 patients (51 males and 52 females) with CBCT imaging data from September 2015 to August 2016, Shanxi Medical University, department of radiology, Mimics 17.0 software was used to measure pulp volume of maxillary canines and tooth volume, and to calculate the ratio of pulp volume/tooth volume.The data were processed by SPSS16.0 statistical software and Fisher discriminant method. The gender determination function was obtained and Cross -Validated method for performance evaluation. Results When gender determination was determined by tooth volume (TV) and pulp volume/tooth volume (PV / TV) as the study index, the gender discriminant function was Y=0.009(TV)+28.896(PV/TV) - 6.962, Cross-validated method for the effect of evaluation, to determine the compliance rate: 51 males, the correct rate of 64.7%;52 female, the correct rate of 78.8%. Conclusion The tooth volume (TV) and pulp volume/tooth volume (PV/TV)can be used as a research indicator of gender inference, it provides a new method and avenue for the gender determination in forensic medicine.

Objective To investigate personal identification of mixed seminal stain of two individuals, we combined the detection of genotyping autosomal, Y and X STR and sequencing mtDNA hypervariable Ⅰ (HV Ⅰ ) region. Methods We analyzed autosomal, Y and X STR with commercial kit and separating and sequencing HVⅠfragments of mixed seminal stain from two males by SSCP electrophoresis. Results Four genetic markers of the high amount sample can be obtained when mixed ratio is more than 1:10. When the proportion of two samples is close, the suspect could be excluded or, to some extent, identified by comparing with our results. Conclusion The combined detection of four genetic marker systems can, to some degree, solve the personal identification from mixed seminal stain of two individuals.

Objective To select the best storage temperature and accurate detection way of the blood alcohol content of drunk driving and provide technical support of judging objectively drunk driving behaviors for traffic management department. Methods This study selects EDTA-2 vacuum tubes, take human vein blood after drinking and respectively store at four temperature conditions -20℃、4℃~8 ℃、 25 ℃ normal temperature and high temperature of 35℃~42 ℃.GC method is used by testing 0,3d, 7d, 14d, 21d and 28d blood alcohol content, and the test result statistics and data are compared and analyzed. Results The blood alcohol contents within 0-3d stored at 35℃~42 ℃and 25 ℃temperature remain stable, decrease significantly after 3d (P<0.05); the blood alcohol contents stored at 4℃~8 ℃ temperature in 0~14d is basically stable and decrease significantly after 14d (P<0.05); at -20℃temperature there are no significant differences among the 28d testing results of blood alcohol contents. Conclusions It is suggested that the blood samples should be collected at low temperature and the best preservation temperature of blood samples is -20℃.