Objectives:The time of Carotid intervention for recently symptomatic,severe carotid stenosis which cause a transient ischemic attack or minor stroke is still a controversial issue.Early studies showed that carotid endarterectomy (CEA) caused a high risk if performed within days follow an acute ischemic stroke.However,The National Stroke Strategy posted by UK Department of Health advocated that this situation should be regarded as an emergency procedure,and carotid intervention should ideally be performed within 48 hours.We designed this study to discuss the effect of urgent CEA on operative risk and benefit.Methods: we analyze 12 urgent CEA with primary closure performed during 1996 to 1998.All 12 patients were underwent CEA within 2 weeks,and 2 of them within 2 days.Operative risks and overall benefit from surgery were discussed in relation to the time from the last symptomatic event to CEA.Results: 2 urgent CEA performed in 2 days are recovery uneventful.1 of 12 patients,who underwent urgent CEA within 2 weeks,occurred restenosis after 3 months follow up.No 30-day perioperative recurrent TIA,stroke and death.Conclusions:CEA performed within 2 weeks is feasible and reliable procedure in preventing recurrent TIA and stroke after presenting manifestations.The future aim is to perform CEA within 48 hours after TIA or stroke symptoms.

Objective:To investigate the expressions of S100A4 and MMP- 2 and their correla-tions to invasiveness and metastasis in gastric cancer. Methods:The expressions of S100A4 and MMP- 2 were detected by SP immunohistochemical method in 49 cases of gastric cancer tissue and 20 cases of normal tissue. Results:The positive rate of S100A4 and MMP- 2 (59.18% 、67.35%) was significantly higher in gastric cancer tissue than in normal gastric tissue (0.00% 、20.00% ,P

Objective: To investigate the cinlical features and therapeutic methods of unex-pected gallbladder carcinoma. Methods: A retrospective analysis was performed in 19 cases of unexpected gallbladder carcinoma treated in our hospital from April 2002 to April 2008. Results: Ten cases in early stage were perfomed gallbaldder resection and local lymph node removal, and the average survival time was 18.7 months; 4 cases were perfomed extend radical resection, and the average survival time was 12.3 months; 5 cases were performed palliative resection, and the survival time was less than 6 months. Conclusions: Frozen section examination during opera-tion is key to diagnose unexpected gallbladder carcinoma. Radical resection is the first option once the diagnosis is confirmed, and the method can be decided according to Nevin classification and B ultrosound scaning during opreation.

Objective: To discuss the feasibility of immune tolerance in rat small bowel trans-plantation induced by immature dendritic cells isolated from SD and Wistar rat. Methods: Dendritic cells isolated from liver or bone marrow of inbred strain Wistar and SD rat were cultured for five to nine days. A week before operation, the recipient rats were injected 2?106 inmature DCs from vena dorsalis penis and observed. The models of heterotopic small bowel transplantation (SBT: SD→ Wistar)were established. Acute immuno- rejection levels were evaluated by HE. Results: Varying degrees of rejection were observed in 3, 5, 7 days after operation. Conclusion: Immature DC can induce allogenic immunological tolerance of rat small bowel transplantation.

Objective:To discuss the effect of bone marrow stem cell mobilization on density and function of macrophages in deep vein thrombosis.Methods:Adult SD rats were divided randomly into sham operation group,thrombus group and rhG-CSF group.Analyzed the density change of macrophages in thrombus on the 7th and 14th day after operation and observed the function of macrophages in thrombus by electron microscope.Results:Density of macrophages in thrombus significantly increased in rhG-CSF group,compared with that in thrombus group(P

Objective:To study the differences in angiogenesis and its maturation between the periphery and center. of hepatocellular carcinoma (HCC). Methods:Thirty-one cases proved surgically and pathologically were selected. After surgery, two sections were gotten from the tumor periphery and center. Continuous slices were stained with immunohistochemistry of five antibodies against VEGF, Flk-1, PCNA.The expressions of VEGF,Flk-1, and PCNA, and some vascular parameters were counted including the number, mean area, total area, circumference, diameter, distance between adjacent vessels(DAV), variety index of microvessel and mature vessels. The arterioles , veinlets, mature vessel index and mean perfused fraction (mPF)were also calculated. The differences of the vascular parameters above between the periphery and center were analyzed. Results: The differences in the number, diameter, and variety index of microvessel, the number and total area of mature vessel, the arterioles and the ratio of arteriole or veinlet, mature vessel index and mPF were statistical differences between the periphery and center of the tumor (P

Objective:To investigate the expression of TIP30 in gastric cancer tissues and analyze its impact with angiogenesis. Methods: The expression of TIP30 and CD34-labeled mi-crovessel density (MVD)were measured by immunohistochemical staining in 52 cases of gastric carcinomas and 47cases of Para-cancer tissues. Results: the positive rate of TIP30 was 53.8% and 85.1% in cancerous tissues and para- cancer tissues, there were significant differences between them(?2=11.22,P

Objective:To investigate the expression of survivin and VEGF in cholangiocarcinoma and explore its relation with clinicobiological behavior. Methods: Immunohistochemical techique was adopted in detecting the survivin and VEGF expression in cholangiocarcinoma and normal bile duct tissues. 51 samples of cholangiocarcinoma were collected in Qilu Hospital of Shandong University from January 2003 to December 2005 , and served as the test group, and 10 samples of normalbile duct tissues as control group. Results: 1)The expression rate of survivin in cholangiocarcinoma was 74.5% , whereas in normal bile duct tissue was 0% , the difference was markablely significant (P0.05). 2)The expression rate of VEGF in cholangiocarcinoma was 80.4% , whereas in normal bile duct tissue was 10% , the difference was significant (P0.05) . VEGF expression of the patients with lymph node metastasis was higher than that of group without lymph node metastasis. 3)The expression of both survivin and VEGF, and was 70.6% in the same sample. Conclusion: Survivin and VEGF may play an important role in tumor genesis, tumor progression, infiltration and metastasis of cholangiocarcinoma, and with intimate relation in angiogenesis.

Objective:To study the expression of Survivin,VEGF,p53 and their correlation inbreast cancer, 22 cases of hyperplasia of mammary gland and 10 cases of paracainoma normal tissues by immunohistochemical technique (SP).Results: The positive rates of Survivin were 76.7%,18.2% and 0% in breast cancer, hyperplasia of mammary gland and paracacinoma normal tissues (P

Objective:To investigate the clinical and pathological features of breast cancer of young women.Methods:The clinical and pathological characteristics of breast carcinoma were compared between young women(≤35 years old,181 cases) and elder women(≥60 years old,186 cases).Results:More tumors were found in left breast,and located in the upper outer quadrant.The major pathological type was invasive ductal carcinoma.There were significant differences in tumor size,histological grading,TNM staging and axillary lymph node metastases(P