One hundred unrelated healthy indi- viduals of the Mulao nationality were ty- ped for HLA-A, -B, -C, -DR and -DQ locus antigens. The distribution of HLA class I antigens is similar to that of Zhu- ang, Miao and Yao nationalities in the Guangxi Area and to that of the Han po- pulations in southern China. For example, A2, A9 and A11 are much more frequent in A locus, as well as B40, B13 and Bw46 in B locus and Cw3. Cw1 (including Cx46) and Cw7 in C locus. The antigen B17 sho- wing a lower frequency in Mulao natio- nality than in other populations in sou- thern China is quite especial. The distri- bution of DR and DQ antigens in HLA class II shows still more different between Mulao and Zhuang nationalities. It is not clear whether the origin of the two na- tionalities or other factors give rise to their difference, and it needs further study.

A study of the mutagenieity of SAGS preservative solution for red cells,performed by Ames method, was reported.Result of the study in- dicated that there was no evidence of the positive mutagenicity reaction of SAGS preservative solution for red cells at its 6 doses ranging from 0.02 to 100mg/ml,whether the rat liver microsomal enzymes(S9)were added as metabolic activators or not.That is, the solution was unable to inducethe mutation at gene loci in the strains tested by Ames Method.

The absorption of anti-A and anti-B in reagent sera containing non-ABO antibodies,performed by coupling the ABH substance to se- pharose 4B,was introduced.The sera containing both ABO antibodies and anti-D(11),-C(5),-E(5),-c(6), -e(4),-M(1),-N(1),-s(1),-K(1), -Kp~b(1),-Fy~a(1),-Js~b(1)absorbed by this method,were tested then by the ABO-incompatible cells lacking relevant non-ABO antigens,and the panel red cells(O).As a result,all the ABO antibodies were absorbed exclusively,but all the non-ABO antibodies were preserved fullyThe non-ABO antibodies can't give the false positive reaction and their titer can't decrease after repeated absorp- tion.The advantages of this method and the factors that have influeced on effectiveness of the absorption were discussed.

A case of B, -D- blood groups was found in a Chinese woman whose baby died of haemolytic disease of newborn, Her husband was AB, CCDee blood groups. The unexpected antibodies in her sera were studied. The sera reacted with the red cells of all common Rh blood groups except -D-, (Rh)~(mod) and (Rh)~(null). Both respective and serial absorption and elu- tion tests were done for the ccDEE, CCD ee and ccde red cells. The results show that there are two antibodies in her sera. The anti-e is separable and the anti -Hr_o can react with all of the common Rh blood groups. The results were the same to that reported by Allen and Corocoran in 1958.

Effect of both granulocyte transfusions and antibiotics on the treatment of acute agranulocytosis with severe infections in 9 of 15 patients were reported. However, all 6 patients with agranulocytosis ind- uced by various drugs survived from sev- ere infections. It revealed that granulocyte count of each transfusion was more sign- ificant for the therapeutic effect.

Using the intravenous hightitred hepatitis B immunoglobulin (HBIG) to prevent post-transfusion hepatitis B was reported. Of the 90 recipients, 50 cases were in the HBIG group and 40 cases were in the control group. All together 11 cases of HBV infection were found. There were 2 cases (4%) in the HBIG group and 9 cases (22.5%) were in the control group, with the difference between them being very significant (P

A comparison between 70-blood-donor and 70-health-control blood lipoids,blood composition,and hemorrheological indexes by paired analysis method basedon occupation,sex and age was made.Paired T-test showed that though severalindexes of the blood donors reduced remarkably,they were still within the normalphysiological limits,There were no abnormal findings through physical examinationand ECG,indication that according to the general physique and present diet condi-tions of Chinese population.It is safe to donate 300ml blood once at intervals of three months.

A safe and effective method for separating albumin from autoplasma by heatwas introduced.Immunological analysis,showed that such an albumin solutionhad the same identity as human serum albumin.Determination by acetate cellu-lose electrophoresis showed the purity of the albumin was over 90%.Determination by polyacrylamide gel electrophoresis showed that one of thealbumin monomers was over 97%.Detection by double radial immunodiffusionindicated that there were no any new antigens in it.The recovery of the albuminwas more than 80%.

A specific monoclonal antibody(McAb),which could identfy different conformations of protein C(PC) in the presence or absence of Ca24+ .was selected and coupled with Sepharose-4B gel to form an affinity chromatographic column. When fresh plasma passed through this column.its PC would he adsorbed exclusively,and the obtained effuent was PC-deficient plasma (PCDP). The residual PC in the PCDP gained was

The sample treatment withRivanol-Ferrous ammumium sulfateis a new procedure which we searchedfor in analyzing Tf C subtypes amongnationalities of South China.Thesample subjected to this treatmentand isoelectric focusing gave clearlyvisible bands,reliable typing resul-ts,and good reproducibility.Onecould determine directly Tf C sub-types and their variants without im-munofixation.The pracedure is easyto perform,economically practical,and can be used in the anthropolongy,population genetics analysis,and inthe TF individual identificationand parent-child relation testing inthe forensic science.