1.Study on drug-guide effect of Achyranthes bidentata in Sanmiao pill in arthritic rats.
Bei SUN ; Lin LV ; Zhong-Xiang LU ; Shi-You YANG
China Journal of Chinese Materia Medica 2008;33(24):2946-2949
OBJECTIVETo study the drug-guiding mechanism of Achyranthes bidentata from Sanmiao pill in arthritic rats.
METHODThe rats were treated by Ferud's complet adjuvant and hot water -bath to establish rat adjuvant arthritis model, then the model rats were divided into three groups, model group, Sanmiao pill groups with A. bidentata. (18 g x kg(-1)) and without A. bidentata. (10 g x kg(-1)), respectively. The heart and foot joints were washed and homogenated to determine the berberine concentration by HPCL in different time after ig and the foot edema was tested by volume method. The pathological changes were observed and hemorheologic parameters were also tested.
RESULTThe berberine concentration of foot joint was significantly higher in 2, 4, 6 hour and 14 day in the rats with A. bidentata. The berberine concentration ratio of foot joint and heart was significantly higher in rats with A. bidentata. pharmacodynamic researches showed that A. bidentata could enhance the edema inhibition effect of Sanmiao pill. Hemorheologic researches showed that A. bidentata. could significantly improve the blood viscosity of model rats, the blood high shear viscosity, the blood low shear viscosity and the plasma viscosity were (6.47 +/- 0.57), (9.28 +/- 1.2), (1.94 +/- 0.19) mPa x s respectively.
CONCLUSIONA. bidentata. could facilitate the targeted tissue distribution of berberine. The effect was correlative with its blood viscosity improvement.
Achyranthes ; chemistry ; Animals ; Arthritis ; drug therapy ; Berberine ; chemistry ; pharmacokinetics ; Disease Models, Animal ; Drugs, Chinese Herbal ; chemistry ; pharmacokinetics ; Female ; Humans ; Male ; Random Allocation ; Rats
2.Effect of isopropyl 3-(3,4-dihydroxyphenyl) -2-hydroxypropanoate on rat pulmonary artery smooth muscle.
Jing LI ; Xin MA ; Wei-Jin ZANG
China Journal of Chinese Materia Medica 2008;33(24):2942-2945
OBJECTIVETo investigate the effect of isopropyl 3-(3,4-dihydroxyphenyl)-2- hydroxypropanoate on vascular smooth muscle.
METHODIsolated rat pulmonary artery was perfused and the tension of the vessel was measured, the effect of isopropyl 3-(3, 4-dihydroxyphenyl)-2-hydroxypropanoate on the pulmonary artery precontracted by noradrenaline (NE) and concentration-response curves of 5-hydroxytryptamine (5-HT), endothelin-1 (ET-1), U46619 and KCl was also observed.
RESULTIsopropyl 3-(3,4-dihydroxyphenyl) -2-hydroxypropanoate exerted relaxation effect on the endothelium-intact artery precontracted by NE in a concentration-dependent manner, which was inhibited with denuded endothelium. The right-shift of the concentration-response curves of 5-HT, ET-1, U46619 and KCl.
CONCLUSIONIsopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate have relaxation action on rat pulmonary artery in the way of endothelium-dependance, the mechanism of relaxation action by isopropyl 3-(3,4-dihydroxyphenyl) -2-hydroxypropanoate may be related to calcium channels.
Animals ; Female ; In Vitro Techniques ; Male ; Muscle Contraction ; drug effects ; Muscle, Smooth, Vascular ; drug effects ; physiology ; Propionates ; pharmacology ; Pulmonary Artery ; drug effects ; physiology ; Rats ; Rats, Sprague-Dawley
3.Regulation function of Qingnao drop pilula to MARCKS mRNA express changes in acute cerebral ischemia hippocampus.
Yun-Ling ZHANG ; Qi-Hui ZHANG ; Wen BAI ; Zhen-Yun HAN ; Hong ZHENG ; Jin ZHANG ; Qi-Fu HUANG
China Journal of Chinese Materia Medica 2008;33(24):2938-2942
OBJECTIVETo observe the contribution of Qingnao drop pilula to the alteration of myristoylated alanine-rich C kinase substrate (MARCKS) mRNA expression in acute multi-infarction hippocampus.
METHODRat models of acute multi-infarction were established by injecting the embolus of blood powder through the right external carotid arteryinto the internal carotid artery, rats were randomly divided into five groups (n = 12 in each): normal, sham operation, model, Chinese medicine treatment, and Western medicine treatment. Qingnao drop pilula (133.28 mg x kg(-1)), nimodipine (7.25 mg x kg(-1)) were administered respectively to Chinese medicine treatment group and Western medicine treatment group by gavage, equal volume of normal saline were given to three groups. Rats were treated with drugs starting at 3rd day before the operation, one time per day. Observing morphologic changes in hippocampus by optical microscope and electron microscope. Detecting expression level of MARCKS mRNA in hippocampus by semi-quantification PCR method.
RESULTHippocampus cells arrange tidy, administrative levels were compactness in normal group, which cells differentially impaired in model group, Chinese medicine treatment group and Western medicine treatment group. Hippocampus cells damage of Chinese medicine treatment group have more reckless than the model group in histopathology. The MARCKS mRNA were expressioned in model group vs medication treatment groups, in Chinese medicine treatment group vs the model group.
CONCLUSIONQingnao drop pilula can alleciate histomorphology lesion of hippocampus when occurring acute multi-infarction, to turn slower MARCKS mRNA expression, may play a neuroprotective effect role through accommodating PKC-MARCKS signal transduction system.
Acute Disease ; Animals ; Brain Ischemia ; drug therapy ; genetics ; metabolism ; Drugs, Chinese Herbal ; administration & dosage ; Gene Expression Regulation ; drug effects ; Hippocampus ; drug effects ; metabolism ; Humans ; Intracellular Signaling Peptides and Proteins ; genetics ; metabolism ; Male ; Membrane Proteins ; genetics ; metabolism ; Myristoylated Alanine-Rich C Kinase Substrate ; Random Allocation ; Rats ; Rats, Wistar
4.Determination of zizybeoside II of Ziziphus jujuba by HPLC.
Ji-Wei NIU ; Qi-Wei ZHANG ; Mu-Xin GONG ; Li-Hua YAN ; Jing-Jing ZHU ; Zhi-Min WANG ; Qin-Fan LI
China Journal of Chinese Materia Medica 2008;33(24):2935-2937
OBJECTIVETo develop a quantitative method for determination of zizybeoside II in Ziziphus jujuba.
METHODThe samples were separated at 30 degrees C on a Zorbax SB-C18 column eluted with methanol-water (20 : 80) as the mobile phase. Flow rate was set at 1.0 mL x min(-1) and the detection wavelength was set at 210 nm.
RESULTThe calibration curve was linear within the range from 0.046 to 0.582 microg (r = 0.999 9) and the average recovery was 97.2%. 12 batches of the crude drugs purchased from different areas were determined and the contents of zizybeoside II in Fructus Jujubae were fluctuated from 0.013% to 0.041%.
CONCLUSIONThe method is simple, repeatable and could be used for the quality control of Z. jujuba.
Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Saponins ; analysis ; Ziziphus ; chemistry
5.Study on fingerprint of Cortex Fraxini with HPLC.
Li-Mei LIU ; Lin CHEN ; Rui-Hai WANG ; Qing YANG ; Xiao-Gang WENG ; Li WANG
China Journal of Chinese Materia Medica 2008;33(24):2932-2935
OBJECTIVETo establish fingerprint of Cortex Fraxini and provide reference for quality evaluation of Cortex Fraxini.
METHODChromatographic experiments were performed on Agilent Extend C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water, containing 0.4% acetic acid as the mobile phases in gradient elution. The detection wavelength was 0-60 min, 340 nm; 6-74 min, 254 nm; 74-75 min, 340 nm, and the flow rate was 1.0 mL x min(-1). Forty samples in four varieties of Cortex Fraxini were detected to establish fingerprints, respectively.
RESULTEvery parameter of the method validation complied with related rules and regulations. There were 15 common peaks in the fingerprint of 10 Fraxinus rhynchophylla samples, eleven common peaks in the fingerprint of 10 F. chinensis var. acuminata. samples, and in the fingerprint of 10 F. chinensis samples. Nineteen common peaks in the fingerprint of 10 F. stylosa samples. There were 5 common peaks in the fingerprints of 40 Cortex Fraxini samples. The similarity factors of the 10 samples of every species were all more than 0.96 compared with the control fingerprint. The similarity of the 40 Cortex Fraxini samples was more than 0.90. Four effective constituents and one unknown constituent were found in 40 samples.
CONCLUSIONThe fingerprints of F. rhynchophylla bark, F. chinensis bark, F. chinensis var. acuminata bark, F. stylosa bark and Cortex Fraxini were established. The methodological evaluation showed that the results were in accord with the technology requirements of chromatography fingerprint, and it laid a good foundation for quality control of Cortex Fraxini.
Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry
6.Study on LC-MS fingerprint for quality assessment of Aristolochia manshuriensis with chemical pattern recognition.
Xia-Lei FAN ; Yi-Bing DING ; A JI-YE ; Xun ZHAO ; Wen-Ying LIU
China Journal of Chinese Materia Medica 2008;33(24):2928-2931
OBJECTIVETo analyze LC-MS fingerprints of Aristolochia manshuriensis for quality assessment with two different chemical pattern recognition models.
METHODLC-MS fingerprints of A. manshuriensis were established from 24 batches of samples from different habitats. SIMCA and Clustering analysis were used to compare the parameters of the 29 common peaks.
RESULTTwo methods had good consistency, while they reflected the inherent sample information from different perspectives, respectively.
CONCLUSIONModern equipment analysis technology and multivariable chemical pattern recognition would be an efficient way for quality control and variety identification of A. manshuriensis.
Aristolochia ; chemistry ; classification ; Chromatography, Liquid ; Cluster Analysis ; Drugs, Chinese Herbal ; chemistry ; Mass Spectrometry ; Phylogeny ; Quality Control
7.Studies on isolation and identification of flavonoids in herbs of Agrimonia pilosa.
Ya PAN ; Hong-Xia LIU ; Yu-Lei ZHUANG ; Li-Qin DING ; Li-Xia CHEN ; Feng QIU
China Journal of Chinese Materia Medica 2008;33(24):2925-2928
OBJECTIVETo study the chemical constituents in Agrimonia pilosa.
METHODThe compounds were isolated and purified by various column chromatographic methods and elucidated on the basis of chemical and spectroscopic evidences.
RESULTNine flavonoids were obtained and identified as tiliroside (1), kaempferol 3-O-alpha-L-rhampyranoside (2), quercetin 3-O-alpha-L-rhampyranoside (3), quercetin 3-O-beta-D-glucopyranoside (4), kaempferol 3-O-beta-D-glucopyranoside (5), kaempferol (6), apigenin (7), luteolin (8), quercetin (9).
CONCLUSIONCompounds 1-3, 5, 6 and 8 were isolated from this plant for the first time.
Agrimonia ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Flavonoids ; chemistry ; isolation & purification
8.Phenolic components from herbs of Dendrobium aphyllum.
Chao-Feng ZHANG ; Li SHAO ; Wei-Hua HUANG ; Lei WANG ; Zheng-Tao WANG ; Luo-Shan XU
China Journal of Chinese Materia Medica 2008;33(24):2922-2925
OBJECTIVETo study the phenolic constituents of Dendrobium aphyllum.
METHODVarious chromatographic techniques were used to isolate and purify the constituents, their physico-chemical properties and spectral data were employed to elucidate their structures.
RESULTNine bibenzyls and two benzylethanyl compounds were isolated and identified as: moscatilin (1), gigantol (2), batatasin (3), tristin (4), 3, 5, 4'-trihydroxylbibenzyl (5), 3, 5-dimethoxyl-4, 4'-dihydroxylbibenzyl (6), moscatin (7), 2, 4, 7-trihydroxyl-9, 10-dihydrophenanthrene (8), hircinol (9), 2-(4-hydroxyphenyl) ethyl-beta-D-glucopyranoside, salidroside (10) and p-hydroxylbenzylacetic acid (11).
CONCLUSIONAll compounds were obtained firstly from the plant, and the compounds 10 and 11 were isolated in this genus for the first time.
Dendrobium ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Phenols ; chemistry ; isolation & purification
9.Studies on chemical constituents in roots and rhizomes of Notopterygium incisum.
China Journal of Chinese Materia Medica 2008;33(24):2918-2921
OBJECTIVETo study the chemical constituents in the roots and rhizomes of Notopterygium incisum.
METHODThe chemical constituents were isolated by various column chromatographic methods and structurally elucidated by NMR and MS evidences.
RESULTTwenty-four compounds were obtained and identified as isocalamenediol (1), pregnenolone (2), beta-sitosterol (3), bergapten (4), bergaptol (5), cnidilin (6), isoimperatorin (7), bergamottin (8), demethylfuropinnarin (9), nodakenetin (10), 7'-O-methylnotoptol (11), notopterol (12), notoptol (13), pabulenol (14), phenethylferulate (15), p-hydroxyphenethyl anisate (16), trans-ferulic acid (17), nodakenin (18), sucrose (19), 2-methoxy-4-(3-methoxy-1-propenyl)-phenol (20), 3, 4, 5 trimethoxy-trans-cinnamic acid (21), p-methoxycinnamic acid (22), 4-acetoxy-3-methoxy-trans-cinnamic acid (23), and p-hydroxy-trans-cinnamic acid (24).
CONCLUSIONCompounds 1, 14, 20-24 were isolated from the roots and rhizomes of N. incisum for the first time. Compounds 1 and 14 are sesquiterpenoid and coumarin, respectively, and 20-24 were phenylpropanoid compounds.
Apiaceae ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; Rhizome ; chemistry
10.Chemical constituents from dried sorophore of cultured Cordyceps militaris.
Zi-Ming LV ; Yong-Tao JIANG ; Li-Jun WU ; Ke LIU
China Journal of Chinese Materia Medica 2008;33(24):2914-2917
OBJECTIVETo investigate the chemical constituents of the dried sorophore of cultured Cordyceps militaris.
METHODCompounds were isolated and purified by macroporous adsorption resin and silica gel column chromatography. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data (IR, FAB-MS, 1H-NMR and 13C-NMR).
RESULTNine compounds were isolated and identified as: ergosta-4, 6, 8 (14)-tetraen-3-one (1), citrostadienol (2), tetracosanoic acid 2, 3-dihydroxypropyl ester (3), ergosterol (4), ergosterol peroxide (5), ergosta-7, 22-dien-3beta, 5alpha, 6beta-triol (6), cordycepin (7), adenosine (8), N-(2-hydroxyethyl) adenosine (9), respectively.
CONCLUSIONCompounds 1-3, 6, 9 were separated from the sorophore of cultured C. militaris for the first time.
Cordyceps ; chemistry ; Culture Techniques ; Fruiting Bodies, Fungal ; chemistry