1.Impact on membrane separation process of volatile oil-bearing water body of notopterygii rhizoma et radix by inorganic salting pretreatment.
Jie SHEN ; Zhifeng HAN ; Liwei GUO
China Journal of Chinese Materia Medica 2011;36(4):425-428
OBJECTIVETo explore the best pretreatment condition of inorganic salting on volatile oil-bearing water body of Notopterygii Rhizoma et Radix before membrane separation.
METHODThe simulative system of volatile oil-bearing water body of Notopterygii Rhizoma et Radix was pre-treated before membrane separation by salting. The best conditions of salting were determined by selecting types and amounts of inorganic salt as investigate factors and comparing membrane flux and oil retention rate.
RESULTThe best pretreatment condition of inorganic salting on volatile oil-bearing water body of Notopterygii Rhizoma et Radix before membrane separation was to add 2% sodium chloride (NaCl) according to the amount of oil-bearing water body. Gas chromatographic fingerprint showed that inorganic salting did not affect the active ingredient of volatile oil.
CONCLUSIONAdding NaCl to volatile oil-bearing water body of Notopterygii Rhizoma et Radix before membrane separation can optimize membrane processes by improving membrane flux.
Apiaceae ; chemistry ; Oils, Volatile ; isolation & purification ; Sodium Chloride ; pharmacology
2.Study on modules biomass structure of Epimedium acuminatum in different habitats.
Weike JIANG ; Tao ZHOU ; Aijuan SHAO ; Meilan CHEN ; Renyin LI ; Yanlei JIN
China Journal of Chinese Materia Medica 2011;36(4):420-424
OBJECTIVETo study the biomass structure of Epimedium acuminatum Franch in the different ecological environments for the development and protection.
METHODThrough the scientific investigation in four typical habitats, the sampling spots were set up, the functional modules' biomass structure and relationship of E. acuminatum were researched.
RESULTThe average of rhizome, as the largest biomass, and the average total biomass had the same pattern: the open areas of forest edge > shrub lumber > shrub-weed > stream drains. The ratio of the functional modules' biomass had different rates under different habitat conditions. By analyzing and combining investigation, the aerial part of E. acuminatum in the shrub-weed were comparative advantage, and the roots of nutrient accumulation of E. acuminatum in the open areas of forest edge were the highest efficient. Under different ecological condition, the distribution of water metabolism was different strategies.
CONCLUSIONIn the open areas of forest edge, E. acuminatum growing well, followed by shrub-weed. These two habitats are the ideal ecological environments while the rhizome or the whole plant used as medicine, but also the protection of E. acuminatum.
Biomass ; Ecosystem ; Epimedium ; growth & development
3.Analysis of genetic differentiation and phylogenetic relationship between Whitmania pigra and Hirudo nipponia based on ITS sequence.
Fei LIU ; Qiaosheng GUO ; Hongzhuan SHI ; Tian WANG ; Shusong LU
China Journal of Chinese Materia Medica 2011;36(4):414-419
OBJECTIVETo evaluate the genetic differentiation and phylogenetic relationship between Whitmania pigra Whitman and Hirudo nipponia Whitman.
METHODBy the sequences of the rDNA internal transcribed spacer (ITS) the molecular phylogenetic tree was constructed by MP method using software MEGA 4.0.
RESULTThe average length of ITS was 857.2-861.2 bp. The A, T, G and C contents in this fragment were 25.12%, 28.28%, 17.34%, 29.29%, respectively. The GC content was higher than the AT content. Little sequence variation was observed in ITS gene fragments with in species, and transition in only 45 loci was revealed in 14 populations. 14 W. pigra and H. nipponia populations were clustered into 2 groups by MP phylogenetic tree.
CONCLUSIONThe results also showed that the intraspecific variation was dominated in variation types of W. pigra and H. nipponia. The classification result of W. pigra and H. nipponia and its adulterants based on DNA sequences are not totally consistent with those based on classification. It showed that a little of mutation of base in ITS sequences had occurred in the process of evolution, such as transition cites, transvertion cites among base or base gap.
Animals ; Base Sequence ; DNA, Ribosomal Spacer ; chemistry ; Leeches ; classification ; genetics ; Molecular Sequence Data ; Phylogeny
4.Preliminary study of reproductive allocation in Cistanche deserticola.
Xia WANG ; Jun CHEN ; Rong XU ; Yougang LIU ; Tongning LIU
China Journal of Chinese Materia Medica 2011;36(4):409-413
OBJECTIVEDiscuss the trade-off between vegetative growth and reproductive growth.
METHODVegetative modules and reproductive modules by large sampling were separated and quantitative charecters of organs and pattern of reproductive allocation were analysed of reproductive allocation were analysed.
RESULTThe phenotype plasticity of quantitative charecters were significant. Reproductive biomass was increasing at different stages. The mass of reproductive investment increased significantly with increasing individual size, the opposite of reproductive allocation. There were all significant positive correlations between the seeds weight and height, total biomass, vegetative biomass, reproductive biomass, fruit numbers, fruit weight.
CONCLUSIONThe significance of phenotype plasticity is the cause of multiple elements. In the process of transferring from vegetative growth to reproductive growth, there was a trade-off between organs. Under individual growth and reproductive strategy, the variation of biomass was allocated coordinately.
Biomass ; Cistanche ; growth & development ; Phenotype ; Reproduction
5.Research on abatement measures of allelopathic autotoxicity of Rehmannia glutinosa.
Juan LI ; Jian HUANG ; Zhongyi ZHANG ; Miaomiao NIU ; Huamin FAN ; Haiyan HE
China Journal of Chinese Materia Medica 2011;36(4):405-408
OBJECTIVETo explore effects on allelopathic autotoxicity of Rehmannia glutinosa of different abatement measures.
METHODThe experiments for abating allelopathic effects of R. glutinosa were designed with the reducing rate as a indication, three measures of the treating soil with lime nitrogen, and water washing were employed, R. glutinosa- Achyranthes bidentata rotation system and using Achyranthes bidentata as green manure, and seedling transplant were also studied for the abatement effect.
RESULTEffect of seedling transplant was the best, reducing rate of transplant and bare root transplant on the 20th day after emergence was 76.80%, 71.70%, respectively. The reducing rate of the treating soil with microorganism reached 54.25%. The effects of water washing and R. glutinosa-A. bidentata rotation system and using A. bidentata as green manure were not satisfied. Especially only using A. bidentata as green manure without rotation system worsened the allelopathic effects of R. glutinosa.
CONCLUSIONAll measures can abate allelopathic effects of R. glutinosa to some extent except only using A. bidentata as green manure.
Crops, Agricultural ; Rehmannia ; growth & development ; toxicity ; Seedlings ; physiology ; Soil Microbiology
6.Review on experiment of traditional Chinese medicine treating to osteoporosis.
China Journal of Chinese Materia Medica 2011;36(4):401-404
Searched the articles between 2000 and 2010, found out and summarized the articles with the topic on the experiment and new techniques of traditional Chinese medicine treating to osteoporosis. The preventive and therapeutic effect to osteoporosis by traditional Chinese medicine had been developed in the past 10 years. The study on standardization of experimental drugs, and the mechanism study with modern cell culture techniques should be enhanced.
Animals
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Humans
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Medicine, Chinese Traditional
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Osteoblasts
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cytology
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drug effects
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Osteoclasts
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cytology
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drug effects
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Osteoporosis
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drug therapy
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Tissue Engineering
7.Advances in determination of multi-residue pesticides in traditional Chinese medicine by GC-MS.
Dongjing LIU ; Jian XUE ; Xiaobo WU
China Journal of Chinese Materia Medica 2011;36(4):396-400
The current methods of preparation of pesticide residue analysis in traditional Chinese medicine were summarized in this paper. And the new preparation techniques used in recent years were reviewed, which included solid-phase micro-extraction (SPME), QuECHERS, matrix solid-phase dispersion (MSPD). In addition, the determination method of the pesticide residue methods in the traditional Chinese medicine were also included in the paper, and analysed the problem in the determination based on the characteristics of TCMs.
Gas Chromatography-Mass Spectrometry
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methods
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Medicine, Chinese Traditional
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Pesticide Residues
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analysis
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Solid Phase Extraction
8.Establishment and research progress of food pharmacy.
China Journal of Chinese Materia Medica 2011;36(4):391-395
OBJECTIVETo introduce the establishment of food pharmacy research progress.
METHODTo overview research situations of food pharmacy development according to edible product.
RESULTFood pharmacy development in China has been set up preliminarily, and needs to be developed further.
CONCLUSIONThe trend of the functional food will be safe, effective and its quality will be control.
Food Analysis ; Food Technology ; Pharmacy
9.Review: autotoxicity in medicinal plants and means to overcome.
Yuechun SUN ; Shufang LIN ; Luqi HUANG ; Xiaobo ZHANG ; Lanping GUO
China Journal of Chinese Materia Medica 2011;36(4):387-390
The review deals with the phenomenon of autotoxicity in medical plants. The autotoxic potential could be attributed to direct inhibition of plant growth and some diseases could be promoted by autotoxin. Factors affecting autotoxicity include species and cultivars, soil microbes, plant's nutrient situation and soil type etc. Autotoxicity could be overcome or alleviated by plant residues removal, adding beneficial microbes, using organic fertilizer, proper rotation, and grading management to different plant' autotoxic force.
Fertilizers
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Plants, Medicinal
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growth & development
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toxicity
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Soil Microbiology
10.Exploration and practice of open experiment in molecular pharmacognosy.
Xiaogang WANG ; Jinbo FANG ; Jia YAN ; Jiachun CHEN
China Journal of Chinese Materia Medica 2011;36(3):383-386
In order to improve quality of molecular pharmacognosy teaching, the open experiment is applied. Under the guidance of tutors, students conduct the whole experiment independently. Students' abilities of independent thinking and comprehensive-experimental conduction were enhanced in the open experiment. Meanwhile, the authors discuss the problems of open experiment and propose some reflection and suggestions.
Education, Medical, Undergraduate
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economics
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manpower
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methods
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standards
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Humans
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Pharmacognosy
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education