1.STUDYING MATHEMATICAL MODELS OF THE TEMPERATURE CONTROL ON FERTILITY OF THE HAEMAPHYSALIS LONGICORNIS
Journal of Chongqing Medical University 1986;0(02):-
By the method of system identification, in this paper the mathematical models of the temperature control on fertility of the hasmaphysalis longicornis is established, and some predictions and estimations are given. The differences' between, the models and other models in the siminlar area are that,the models are coatinuous and differantiable other than segmented and undifferentiable
2.Category research of nursing outcomes and indicators of hospitalized patients with coronary disease
Ya YANG ; Xinjuan DAI ; Zhongqin XU
Chinese Journal of Practical Nursing 2015;31(15):1093-1096
Objective To carry on the theoretical research of nursing outcomes and indicators of hospitalized patients with coronary heart diease (CHD).Methods Self-designed questionnaire survey was conducted according to the Nursing Outcomes Classification,and then using it among 14 cardiology nursing experts.Results After experts questionnaire,determined 13 nursing outcomes,63 major indicators and 38 secondary indicators,while eliminated 9 indicators.Conclusion Formation of nursing outcomes and indicators of hospitalized patients with CHD is practical,professional,systemic and simplicity,it can be used as a tool to guide the clinical nursing practice.
3.Elimination Effect of Radix Pulsatillae Liquid Extracts on R Plasmid in Pseudomonas Aeruginosa
Qiang MIAO ; Yang XIAO ; Zhongqin HE
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(04):-
Objective To explore the elimination effect of radix Pulsatillae extracts on R plasmid of pseudomonas aeruginosa.Methods R plasmids of pseudomonas aeruginosa were isolated from the sputum of elderly patients suffering from respiratory infection,and the elimination test of radix Pulsatillae extracts on these plasmids was performed in vitro and in vivo.Results Being treated with the extracts for 24,48 and 72 h in vitro,the elimination rate of R plasmids was 0,1.8 %and 3.4 %respectively,while in SDS(sodium dode canesulphonate)control group the elimination rate was 0,0.8 %and 1.6 %respectively.Being treated with the extracts for 24,48 and 72 h in vivo,the elimination rate was 0,2 %and 12.8 %respectively,and 0 in the control group.Conclusions Both in vitro and in vivo,radix Pulsatillae extracts have an elimination effect on R plasmid of pseudomonas aeruginosa,and the effect in vivo is stronger than that in vitro.
4.The research progress of cobra venom secretory phospholipase A_2
Yaping YANG ; Zhongqin LIANG ; Zhenlun GU ; Zhenghong QIN
Chinese Pharmacological Bulletin 2003;0(09):-
Cobra venom secretory phospholipase A_2 (sPLA_2) is an important component of cobra venom which has a variety of biological activities. Recent studies are mainly focusing on each pharmacological active component of venom, SPLA_2 is one of them. This review summarized the structure, purification and biological activities of cobra venom sPLA_2 with emphasizing its diverse pharmacological effects and toxicity. In addition, some mechanisms of actions of sPLA_2 and possible applications of sPLA_2 were also discussed.
5.Regulation Effect of Water-extraction from Ganoderma Lucidum (Lyess.ex Fr) karst. on Human Immune cells and IL-6
Zhongqin LIANG ; Weihua SHENG ; Zhenrun GU ; Jicheng YANG ;
Chinese Traditional Patent Medicine 1992;0(12):-
Objective:To study the regulation effect of water extraction from Ganoderma Lucidum (Lyess. ex Fr) karst (GL W) on human immune cells and cytokine. Methods: With the technologies of MTT and FACS, the proliferation of T lymphocyte subgroup and IL 6 production in human peripheral blood lymphocytes (PBL) were studied. Results: 1. In the absence of mitogin, GL W could induce not only the inactive lymphocyte but also the PHA activited lymphocyte to proliferate in the similar concentration. The proliferation in the later was more obvious ( P
6.Induction of apoptosis and inhibition of telomerase activity by extract of Fagopynum cymosum(Fr4) in human leukemia HL-60 cells
Xiaofeng CHEN ; Zhenlun GU ; Haihua YANG ; Zhongqin LIANG ; Min ZHU ; Baoguo CHEN
Chinese Pharmacological Bulletin 2003;0(07):-
Aim To study effect of the extract of Fagopynum cymosum(Fr4) on the apoptosis and telomeraseactivity in human leukemia HL-60 cells.Methods HL-60 cells were treated with Fr4 and inhibition of proliferation was measured with MTT assay.Cell morphology before and after the treatment was examined with light and electron microscopy.FILC-Annexin-V and PI double staining was used to detect cell membrane-mediated apoptosis by FCM.DNA fragmentations were analyzed with DNA gel electrophoresis.Furthermore,telomerase activity was determined with PCR-ELISA-based telomeric repeat amplification(TRAP).Results The proliferation of HL-60 cells was inhibited by Fr4 with an IC_(50) of 115 mg?L~(-1).Typical morphology and biochemical feature of apoptosis was observed with Fr4 60~240 mg?L~(-1).The percentages of early apoptosis cells were increased in a dose-dependent manner.Telomerase activity was decreased in a dose-dependent manner during the process of apoptosis.Conclusion Fr4 induced apoptosis in HL-60 cells.Down-regulation of the telomerase activity in HL-60 cells might be contributed to Fr4-induced apoptosis.
7.Construction of nursing standard operation procedures in department of cardiology
Zhongqin XU ; Ya YANG ; Lan ZENG ; Hui DAI ; Xiaoyan YU ; Xiaolu LI ; Xinjuan DAI
Chinese Journal of Practical Nursing 2015;31(1):30-32
Objective We sought to establish the standard operation procedures in department of cardiology.Methods According to the method of constructing standard operation procedures,the standard operation procedures for the department of cardiology was constructed through induction and consulting literature materials.Results 16 standard operation procedures for the department of cardiology were developed,including 8 SOP of common diseases to rescue,2 SOP of common operation in perioperative period,4 SOP for common instruments,2 SOP for common drugs usage.Conclusions Construction of standard operation procedures in department of cardiology can provide the basis for clinical nursing work,it can also supply methodological reference to build the standard operation procedures in other departments.
8.Three kinds of viruses isolated from diarrhea patients of infants and preschool children in Fuzhou city.
Jian-min GAO ; Tao WANG ; Jun-lin CHEN ; Jun-yang CHEN ; Yu LIN ; Zhongqin CAI
Chinese Journal of Epidemiology 2004;25(5):456-457
Adenovirus Infections, Human
;
epidemiology
;
virology
;
Adenoviruses, Human
;
isolation & purification
;
Caliciviridae Infections
;
epidemiology
;
virology
;
Child, Preschool
;
China
;
epidemiology
;
Diarrhea
;
epidemiology
;
virology
;
Diarrhea, Infantile
;
epidemiology
;
virology
;
Enzyme-Linked Immunosorbent Assay
;
Feces
;
virology
;
Female
;
Gastroenteritis
;
epidemiology
;
virology
;
Humans
;
Incidence
;
Infant
;
Male
;
Norwalk virus
;
isolation & purification
;
Reverse Transcriptase Polymerase Chain Reaction
;
Rotavirus
;
isolation & purification
;
Rotavirus Infections
;
epidemiology
;
virology
9.Effects of electrical stimulation on the differentiation of mesenchymal stem cells into cardiomyocyte-like cells.
Zhongqin YAN ; Gang YANG ; Liao CUI ; Xueling HE ; Wei KUANG ; Wenchao WU ; Xiaojing LIU ; Liang LI
Journal of Biomedical Engineering 2013;30(3):556-561
The aim of this study is to investigate the effects of electrical stimulation ES) on the induction of rat bone marrow mesenchymal stem cells (rBMSCs) to differentiate into cardiomyocyte-like cells in vitro. The third or fourth-generation of the rBMSCs was randomly divided into three groups, i. e. ES group, 5-Azacytidine (5-Aza) group, and control group. Those in the ES group with complete medium were exposed to 1,2,4 and 6V, 2Hz, 5ms ES for 2h everyday,lasting for 10d. Those in the 5-Aza group were induced by 10 micromol/L 5-Aza for 24h, then the medium was changed to complete medium without 5-Aza. Those in the control group were only cultured with complete medium. The growth status and morphological features of rBMSCs were observed by inverted phase microscope. The mRNA expressions of GATA4, a-actin, ACTN2 and TNNT2 were determined by Real-time fluorescent quantification PCR, and the protein expression of TNNT2 was detected with immunofluorescence staining. The results showed that the mRNA expression level of the GATA4, a-actin, ACTN2 and TNNT2 and the protein expression level of the TNNT2 were significantly higher in the ES group and 5-Aza group, compared to those in the control group(P<0. 05). It suggested that ES could induce rBMSCs differentiation into cardiomyocyte-like cells in vitro.
Animals
;
Bone Marrow Cells
;
cytology
;
Cell Differentiation
;
Cell Proliferation
;
Cells, Cultured
;
Electric Stimulation
;
Female
;
Male
;
Mesenchymal Stromal Cells
;
cytology
;
Myocytes, Cardiac
;
cytology
;
Rats
;
Rats, Sprague-Dawley
10.A clinical study of clinical cure after the addition of interferon therapy in chronic hepatitis B patients with low-level HBsAg previously treated with nucleos(t)ide analogues
Weili NIU ; Yongsu WANG ; Qingshan WU ; Lin ZHANG ; Zhongqin ZHANG ; Xiaojun YANG ; Xianbin ZHU ; Wenqin XIAO ; Mingping JI
Journal of Clinical Hepatology 2021;37(8):1793-1797.
ObjectiveTo investigate the population with an advantage of clinical cure previously treated with nucleos(t)ide analogues (NAs), and to provide more methods for clinicians in pursuing the clinical cure of hepatitis B. MethodsA total of 42 chronic hepatitis B patients with low-level HBsAg who received NAs treatment in Hebi Third People’s Hospital from October 2017 to October 2019 were enrolled as subjects and divided into combination treatment group (group A) and NA monotherapy group (group B). The 22 subjects in group A were treated with NAs combined with PEG-IFN antiviral therapy for 48 weeks, and some patients withdrew from PEG-IFN after 24 weeks and continued to receive NA monotherapy, while the 20 subjects in group B received NA antiviral therapy alone. Both groups were observed till week 48, and the five makers for hepatitis B were measured to evaluate clinical outcome. The t-test was used for comparison of continuous data between two groups, and the Fisher’s exact test was used for comparison of categorical data between two groups; a multivariate logistic regression analysis was used to perform a multivariate analysis. ResultsCompared with group B at the 48-week treatment endpoint, group A had significantly higher HBsAg clearance rate (45.5% vs 0, P<0.01) and HBsAg seroconversion rate (31.8% vs 0, P<0.01). The population with HBsAg <1000 IU/ml, <500 IU/ml, <100 IU/ml, and <10 IU/ml had an HBsAg clearance rate of 52.6%, 61.5%, 66.7%, and 100%, respectively, and the population with an HBsAg level of 500-1000 IU/ml, 100-500 IU/ml, 10-100 IU/ml, and <10 IU/ml had an HBsAg clearance rate of 33.3%, 50%, 40%, and 100%, respectively. The 4 patients with baseline HBsAg <10 IU/ml (accounting for 18.2% in group A) achieved clinical cure at week 12 of combined treatment, and after observation to week 48, 2 patients had an anti-HBs level of >100 IU/ml and 2 had an anti-HBs level of >1000 IU/ml. The multivariate logistic regression analysis of HBsAg clearance showed that age at the initiation of combined treatment affected HBsAg clearance (odds ratio [OR]=0.877, 95% confidence interval [CI]: 0.781-0.985, P=0.026), and most of the patients with HBsAg clearance had an age of 36-49 (44.20±4.49) years; baseline HBsAg level also had an impact on HBsAg clearance (OR=0.996, 95% CI: 0.992-1.000, P=0.050). ConclusionThe addition of interferon therapy in chronic hepatitis B patients with low-level HBsAg previously treated with NAs can significantly improve the clinical cure rate. The younger the age and the lower the HBsAg level, the shorter the duration of combined treatment. Age and baseline HBsAg level are more important than the duration and type of NA medication.