Objective To know the effect of water supply improvement on prevention and control of the endemic arsenism. Methods 35 residents aged 45-65 years (21 male, 14 female) in a high arsenic area were selected for determination of urine arsenic, 21 residents aged 45-65 years (14 male, 7 female) in another high arsenic area for determination of hair arsenic and the residents in the control area were also selected. Results 16 years after the water supply improved (concentration of arsenic

OBJECTIVE:To observe clinical efficacy and safety of Zishen jianpi huoxue decoction combined with Xiaoxintong capsule in the treatment of type 2 diabetes complicating with silent myocardial ischemia (SMI). METHODS:126 patients with type 2 diabetes complicating with SMI were selected and randomly divided into control group(62 case)and observation group(64 cases). Both group were given diet guidance and hypoglycemic drugs as sulfonylureas,biguanides. Control group was given Xiaox-intong capsule 10 mg,tid;observation group was additionally given Zishen jianpi huoxue decoction 400-500 ml,bid. Both groups received treatment for 7 d. Therapeutic efficacy of thoracic obstruction,improvement of coronary artery branch stenosis and times and duration of electrocardiogram ST segment low voltage outlet were observed in 2 groups. ADR was compared between 2 groups. RESULTS:Therapeutic efficacy of thoracic obstruction and improvement of coronary artery branch stenosis in observation group were significantly better than in control group,with statistical significance(P<0.05);the times and duration of electrocardiogram ST segment low voltage outlet in observation group were significantly lesser shorter than in control group,with statistical signifi-cance (P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Zishen jianpi huoxue decoction complicating with Xiaoxintong capsule shows significantly therapeutic efficacy in the treatment of type 2 diabetes complicating with SMI with good safety.

Objective:To explore the influencing factors on patients' health seeking behavior in rural China by employing the theory of planned action.Methods:Data from cross-sectional household-based health survey carried out in 2008 were used in the study.Correlations between outcomes and explanatory variables were studied by Logistic regression.Results:The studying population included 19 389 adult patients over the age of 15.Illness awareness,medical experience and structural restriction factors had strong effect on whether the patients chose medical service.Pepople in different socioeconomical status suffered different influences.Conclusion:Responding to the changing trend of health service demand among rural residents,we should recognize the differentiation and allocate the healthcare resources more rationally.

Objective To study the effects of directional inducement of factors produced by injured sciatic nerve on the regenerating axons of adult frog in vivo. Methods Conditioned medium with fraction of 30kD to 100kD was obtained from sciatic nerve of adult frog and pumped out and formed a gradient of concentration toward the end of central nerve stump of cutaneous pectoris nerve with different distances and angles by miniosmotic pumps.After 28\|35 days,the chest tissue was dissected and stained with immunocytochemistry to show the regenerated motor\|axons and the directional value is analyzed. Results The regenerated motor axons grew obviously towards the tip of the cannula with high concentration of CM.The directional value is 0\^84 whereas the value is 0\^04 with random direction.The average directional value between the two groups is significant and is not influenced by the distances and angles of the cannula to the nerve stump. Conclusion Damaged peripheral nerve can produce diffusible active factors with molecular weight between 30kD to 100kD,which induce the directional outgrowth of regenerating motor axons from sectioned nerve stump of adult frogs in vivo. [

Objective: To investigate the problems and improve the quality of experimental teaching for medical laboratory instruments.Method: A questionnaire was made among 100 laboratory medicine students in Chongqing University of Medical Sciences.Result: About eighty-six percent of the students were satisfied with the experimental teaching for medical laboratory instruments,but there were still problems: absence of clear objectives in experimental teaching;shortage of activity of medical laboratory instruments experiment;lack of modern large-scale instruments.Conclusion: the key points to improve experimental teaching quality are to set up clear experimental teaching objective;update instruments for experimental teaching;structure simulator laboratory with modern education simulator technology.

Students will be familiar with the major contents of clinical laboratory management,such as clinical laboratory quality management,laboratory creature safety containment,clinical laboratory accredit action through the course of clinical laboratory management for laboratory medicine students.Meanwhile,by continuously perfecting the teaching contents and methods,students' satisfaction with this course has been improved,and they pay more attention and give higher appraisement to this course.

BACKGROUND:Diabetic lower limb ischemia is prone to involve distal lower limb arteries, and a conventional treatment is often unable to obtain the ideal effect. OBJECTIVE:To investigate the effect and safety of umbilical cord blood stem cel transplantation in the treatment of diabetic lower limb ischemia. METHODS: A diabetic rat model of lower limb ischemia was established, and along the femoral artery, five points
were selected for injection of human umbilical cord mesenchymal stem cel suspension, 20 μL per point. At 1, 2, 4 weeks after transplantation, transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region, and incidence of adverse reactions were recorded. RESULTS AND CONCLUSION:At 1, 2 and 4 weeks after transplantation, the transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region were found increasing, which were significantly different from those before transplantation (P < 0.05). At different time after transplantation, al animals had no inflammatory reactions such as skin bleeding and dermatitis, and local red, sweling, hot, pain, and had no tumor-like growth in organs. These findings indicate that umbilical cord blood stem cel transplantation can safely and significantly improve symptoms of diabetic lower limb ischemia, which has certain application feasibility. Cite this article:Xie LH, Xing L, Zheng H. Feasibility of umbilical cord blood stem cel transplantation for the treatment of diabetic lower limb ischemia. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):78-82.

Objective To investigate the effects of β adrenergic receptor blocker carvedilol and cholinergic receptor blocker scopolamine on the biological behaviors of prostate cancer in C57BL/6 mice.Methods From June 2014 to November 2014, sixty male C57BL/6 mice, 10 weeks old, were injected with RM-1 cells (0.5 × 106) into bilateral dorsal prostate capsules of each mice by microsyringe and were randomly divided into three groups, including carvedilol group (K), scopolamine group (D) and saline group (N) after modeling.In K group, the carvedilol (10 mg/kg) was given to mice through gastric tube.The normal saline was given to them by subcutaneous injection.In D group, the normal saline was given to mice through gastric tube.Meanwhile, the scopolamine (1 mg/kg) was injected subcutaneously.In N group, daily normal saline was given by through gastric tube and subcutaneous injection.At the seventh day of postoperation, five mouse were sacrificed in each group every three days to observe the local growth,invasion and metastasis of prostate cancer to pelvic nodes and liver.Immunohistochemical determination of prostate tumor was made by TH staining and VAChT staining in each group.Results The volume of the prostate cancer was gradually increased in three groups.There was no statistical difference of prostate volume among three groups on the day 7 and 10 post-operatively (all P ＞ 0.05).The prostate volume of K group, D group and N group on the day 13 were 0.05 ± 0.04, 0.18 ± 0.08, 0.14 ± 0.05 cm3, respectively (P ＜0.05).However, the statistical significance existed between K and N group (P ＜ 0.05).There was no statistical difference of AOD between TH and VAChT among three groups on the day 7 (all P ＞ 0.05).The AOD of TH and VAChT in prostate cancer of K group, D group and N group on the day 10 were [(0.0114±0.016), (0.114±0.002), (0.059±0.008)] and [(0.025 ±0.011), (0.0226±0.003), (0.009±0.003)], respectively (P ＜0.05).Those values on the day 13 were [(0.147 ±0.036),(0.129 ±0.025), (0.071 ±0.022)] and [(0.020 ±0.005), (0.020 ±0.002), (0.010 ±0.002)],respectively (P ＜0.05).TH and VAChT expression of K and D in 10th and 13th is higher than in N (all P ＜ 0.05).At the 7th day, no metastasis of pelvic nodes was detected in all groups.At 10th day, 2 cases of K, no case of D and 3 cases of N were detected the lymph node metastasis.At the 13th day, 3 cases of K, 1 case of D and 4 cases of N were recorded the lymph node metastasis.At the 7th day, no metastasis of liver was detected.At 10th day, 1 case of K, no case of D and 2 cases of N were found the liver metastasis.At the 13th day, 3 cases of K, 1 case of D and 4 cases of N were recorded the liver metastasis.The lifesapn of tumor-bearing mice in K group, D group and N group was 16.8 ±0.8, 17.6 ±0.5, 15.8 ±0.1, respectively (P ＞ 0.05).Conclusions β adrenergic receptor blocker carvedilol can suppress the growth of prostate cancer and cholinergic receptor blocker scopolamine may can inhibit metastasis of prostate cancer.

Objective:To study the status quo of National Essential Medicine System ( NEMS) in village clinics of a province in western China. Methods:Individual in-depth interview was carried out with village doctors and their managers of a province in western China, to understand the subsidies of zero-markup rate, compensations for general medical fees, and status quo of NEMS assessment mechanism and its impacts on prescribing behavior of village doc-tors. Results:In the surveyed area, the intervention of NEMS has not achieved the desired objectives: the prescri-bing behavior of village doctors has not changed significantly;general medical fee system has failed to achieve reason-able compensations;and the assessment mechanism done by village doctor managers has been a mere formality. Con-clusions and Suggestions:To ensure the feasibility, effectiveness and sustainability of the intervention, the area poli-cymakers should take into full consideration the special nature of village doctors.