1.Molecular Mechanism of GnRH Interaction with GnRH Receptor in an Evolutionary Viewpoint.
Jae Young SEONG ; Hyuk Bang KWON
Journal of Korean Society of Endocrinology 2000;15(6):779-790
No Abstract Available.
Gonadotropin-Releasing Hormone*
;
Receptors, LHRH*
2.The effect of Er:YAG laser irradiation on the surface microstructure and roughness of TiO2 implant.
Jang Hyuk AN ; Young Hyuk KWON ; Joon Bong PARK ; Yeek HERR ; Jong Hyuk CHUNG
The Journal of the Korean Academy of Periodontology 2008;38(1):67-74
PURPOSE: The aim of this study was to evaluate the effect of Er:YAG laser on microstructure and roughness of TiO2 blasting implant surface. MATERIALS AND METHODS: Ten TiO2 blasting implant were used in this experiment. One implant was control group, and nine TiO2 blasting implant surfaces were irradiated with Er:YAG laser under 100 mJ/pulse, 140 mJ/pulse, and 180 mJ/pulse condition for 1 min, 1.5 min, and 2 min respectively. Optical interferometer and scanning electron microscopy was utilized to measure roughness and microstructure of specimens. RESULTS: The surface roughness was decreased after Er:YAG laser irradiation in all groups, but there was no significant difference. 100 mJ/pulse and 140 mJ/pulse group did not alter the TiO2 blasting implant surface in SEM study while 180 mJ/pulse group altered the TiO2 blasting implant surface. Implant surfaces showed melting, microfracture and smooth surface in 180 mJ/pulse group. CONCLUSION: Detoxification of implant surface using Er:YAG laser must be irradiated with proper energy output and irradiation time to prevent implant surface alteration.
Freezing
;
Microscopy, Electron, Scanning
3.I-shaped incisions for papilla reconstruction in second stage implant surgery.
Eun Kwon LEE ; Yeek HERR ; Young Hyuk KWON ; Seung Il SHIN ; Dong Yeol LEE ; Jong Hyuk CHUNG
Journal of Periodontal & Implant Science 2010;40(3):139-143
PURPOSE: Pink gingival esthetic especially on the anterior teeth has been an important success criterion in implant-supported restoration. Inter-implant papillae are a critical factor for implant esthetics, and various techniques for inter-implant papilla reconstruction have been introduced. The aim of this study is to suggest and evaluate a surgical technique for reconstructing inter-implant papillae. METHODS: A 28-year-old man had an implant placed on the #13 and #14 area. Four months after implant placement, a second stage surgery was planned for inter-implant papilla reconstruction. At the time of the abutment connection, I-type incisions were performed on the #13i & #14i area followed by full-thickness flap elevation and connection of a healing abutment on underlying fixtures without suture. RESULTS: Two weeks after the second stage implant surgery, soft tissue augmentation between the two implants was achieved. CONCLUSIONS: I-shaped incisions for papilla reconstruction performed during the second stage implant surgery were useful for inter-implant papilla reconstruction and showed a good esthetic result.
Adult
;
Dental Implants
;
Dental Papilla
;
Esthetics
;
Esthetics, Dental
;
Humans
;
Sutures
;
Tooth
4.Electron microscopy of the oocyte-cumulus complex and immuncytochemistry on the distribution of fibronectin, tenascin, and laminin.
Yu Il LEE ; Ju Eun CHO ; Hyun Jeong PARK ; Young Sook KWON ; Jae Hyuk LEE
Korean Journal of Obstetrics and Gynecology 2000;43(2):192-202
OBJECTIVE: Immunofluorescence microscopy including confocal laser scanning microscopy and electron microscopy were used to study the production of fibronectin, tenascin, and laminin in the cumulus-corona (CC) cells surrounding mature, unfertilized oocytes after ovulation in view of their presumptive importance in the coordination of the processes leading to fertilization and early embryo cleavage, including the final maturation of the ovum, the sperm-egg interaction, and the complex biochemical mechanism between the ovum and the oviduct. METHODS: Mature oocyte-cumulus complex (OCC) was cultured for 24 and 48 hour and fixed in 3.7% formaldehyde. Specimens were incubated with a mixture of primary monoclonal antibodies recognizing different epitopes of fibronectin, tenascin, and laminin, and then with a mixture of secondary antibodies containing FITC, TRITC, and Cy-5 conjugated antibodies. Observation was made by confocal laser scanning microscope equipped with epifluorescece optics. Transmission electron microscopy were used to observe the OCC at 24 and 48 hours after cultrue. RESULTS: The immunocytochemical date demonstrated that CC masses are capable of producing fibronectin and tenascin but their production is heterogeneous in the CC population. Immunoreactivity to fibronectin and tenascin was shown mostly by inner corona cells, and the intensity of immunofluorescence decreased from the central corona cells to the peripheral cumulus cells. Colocalization of fibronectin and tenascin was evident in most CC cells. Moreover, fibronectin and tenascin immunoreactive material was observed in the intracytoplasmic areas, at the plasma membrane level as well as in the extracellular matrix. Whereas, laminin immunofluorescence was found around plasma membrane and extracellular area, but a intracytoplasmic reaction was rarely observed. The distribution of laminin immunofluorescence was similar to that of fibronectin and tenascin, but in some cumulus cells, colocalization between them was not found. Ultrastructurally, cumulus cells projected numerous long, thin microvilli into the intercellular area and some micovilli penetrated into zona pellucida. The inner layer of the cumulus mass was loose arrangement of relatively uniform, small cells with widened intercellular spaces, whereas in the outer layer, cumulus cells are rather larger in size and compact arrangement by narrow, irregular spaces. A small and large linear gap junctions were easily found at cell contacts. The cytoplasm of most cells had abundant organelles typical of steroidogenesis: numerous mitochondrias, a well-developed smooth endoplasmic reticulum, electron dense lipid droplets, and bundles of microtubules and microfilaments. Rudimentary disrupted basal lamina along the cytoplasmic border was rarely seen in a few inner conora cells. CONCLUSION: Even though the functional role of these extracellular matrix proteins remains still unclear, it is reasonable to suggest that they are necessary in various steps of the reproductive process. Cumulus cells appears to be a heterogeneous and dynamic system for suitable microenviroment of fertilization. And functional differences between corona and cumulus cells during the oocyte denudation may be accounted for particular distribution of these adhesive proteins and steroidogenesis-related organelles.
Actin Cytoskeleton
;
Adhesives
;
Animals
;
Antibodies
;
Antibodies, Monoclonal
;
Basement Membrane
;
Cell Membrane
;
Cumulus Cells
;
Cytoplasm
;
Embryonic Structures
;
Endoplasmic Reticulum, Smooth
;
Epitopes
;
Extracellular Matrix
;
Extracellular Matrix Proteins
;
Extracellular Space
;
Female
;
Fertilization
;
Fibronectins*
;
Fluorescein-5-isothiocyanate
;
Fluorescent Antibody Technique
;
Formaldehyde
;
Gap Junctions
;
Immunohistochemistry
;
Laminin*
;
Microscopy, Confocal
;
Microscopy, Electron*
;
Microscopy, Electron, Transmission
;
Microscopy, Fluorescence
;
Microtubules
;
Microvilli
;
Mitochondria
;
Oocytes
;
Organelles
;
Oviducts
;
Ovulation
;
Ovum
;
Sperm-Ovum Interactions
;
Tenascin*
;
Zona Pellucida
5.MRI Study about the Early Changes of Lumbar Disk Degeneration using Magnetization Transfer Contrast (MTC).
Young Soo KIM ; Tae Sub CHUNG ; Tae Hoon KIM ; Eun Kee JEONG ; Hyuk Woo KWON
Journal of the Korean Radiological Society 1995;32(6):865-870
PURPOSE: To obtain magnetization transfer ratio(MTR) of the annulus fibrosus and nucleus pulposus and to assess the feasibility of utilizing the changes of these MTRs as an early indicator of disk degeneration. MATERIALS AND METHODS: MR images of lumbar spine with magnetization transfer(MT) technique in 42 patients were obtained. spin echo techniques (600/14) with same TR/TE with 1KHz off-resonance saturation were employed in 1.0T MR system. MTRs were calculated in two regions, anterior annulus fibrosus and nucleus pulposus, and the results were compared between the normal and degenerative disks, from grade I to IV, on T2-weighted images. RESULTS: MTRs of the nucleus pulposus were 17.6% in the normal disks, and 26.7%, 28.4%, 29.1%, 29.7% in degenerative disks, from grade I to IV, respectively, with a significant difference(P<0.05). On the other hand, MTRs in the annulus fibrosus were 30.2% in the normal disks and 31.5%, 33.2%, 32.1% and 35.6% in degenerative disks, from grade I to IV, respectively, without significant difference. CONCLUSION: Since MTRs are significantly higher in degenerative nucleus pulposus than those of the normal disks, increased MTRs in the nucleus pulposus can be used as an early sign of the degeneration of the nucleus pulposus.
Hand
;
Humans
;
Intervertebral Disc Degeneration*
;
Magnetic Resonance Imaging*
;
Spine
6.A case of congenital tuberculosis.
Mee Hye KIM ; Hyuk Choul KWON ; Young Il PARK ; Sang Woo KIM
Journal of the Korean Pediatric Society 1985;28(2):169-173
No abstract available.
Tuberculosis*
7.Clinical study of interlocking intramedullary nailing on proximal or distal femur and distal tibia fracture.
Kwang Yun SEO ; Chil Soo KWON ; Young Wook KIM ; Jin Hyuk KIM ; Mong Lyog SHIN
The Journal of the Korean Orthopaedic Association 1992;27(3):678-685
No abstract available.
Femur*
;
Fracture Fixation, Intramedullary*
;
Tibia*
8.A Case of Antipsychotic-Regression Syndrome in Haloperidol Treated Tourette's Syndrome.
Hee Yeon JEONG ; Hyun Ju CHO ; Young Joon KWON ; In Joon PARK ; Hyuk Hee JIN
Journal of the Korean Society of Biological Psychiatry 1998;5(1):134-137
Authors report a case of separation anxiety disorder, which developed as a side effect during haloperidol treatment of Tourette syndromes(TS). In this case, 14 years old boys developed attention deficit symptoms during his infancy. At 4th grade of primary school, he developed vocal tic, motor tic, and coprolalia. With 5mg/day of haloperidol treatment his symptoms of TS were subsided. During the treatment, he developed features of separation anxiety disorder, including dependence, pleading, clinging, and sadness. Symptoms of attention deficit and separation anxiety disorder were improved by 25mg/day of imipramine treatment. During haloperidol treatment of TS, careful observation may be needed whether separation anxiety disorder-like symptom develops.
Adolescent
;
Anxiety, Separation
;
Haloperidol*
;
Humans
;
Imipramine
;
Tics
;
Tourette Syndrome*
9.The influence of adjacent tooth to the microbiology of implant sulcus.
Dong Young LEE ; Man Sub LEE ; Young Hyuk KWON ; Joon Bong PARK ; Yeek HERR ; Jong Hyuk CHUNG
The Journal of the Korean Academy of Periodontology 2005;35(3):563-575
The aim of present study is to evaluate the influence of adjacent tooth to the microbiology of clinically healthy implant. Control group included patients who had clinically healthy implant and tooth with healthy periodontium(PD< or =3mm), test group was composed of patients who had clinically healthy implant and tooth with periodontal pocket(PD > 3mm). The criteria of clinically health implant are no pain or discomfort, the restorative suprastructure provide satisfactory fit and function, and the tissue around the fixtures were firm and probing with standard periodontal probe with a rounded tip 0.5mm in diameter resulted in penetration of no more than 5mm when using a force of 0.5N at any location. 38 patients, partially edentulous subjects with endosseous root-form implants were selected. All subjects were medically healthy and had not taken systemic antibiotics and professional plaque control 3 months before sampling. Number of control group is 25(mean age 52+/-13, 26 teeth, 34 implants) and test group is 13(mean age 60+/-13, 13 teeth, 17 implants). All teeth and implants of each patient were examined probing depth(PD), bleeding on probing(BOP), and plaque index(PI), and samples of subgingival plaque were obtained at each site with sterile curet or fine paper points, then the plaque transferred to PBS. Obtained samples were examined for the presence of P. gingivalis, T. forsythensis, and T. denticola by the polymerase chain reaction(PCR). The relationship among clinical parameters and the colonizations by the 3 bacterial species from natural teeth and implants region were analyzed by student t-test. The results of this study were as follows: 1. PD was different in teeth between 2 groups(p<0.05), but the other parameters were not. 2. Statistically significant difference was not found in clinical parameters of implants between 2 groups. 3. All bacterial prevalences of teeth were higher in test group than in control group, and prevalence of T. forsythensis had statistically significant difference between 2 groups(p<0.05). 4. Prevalences of P. gingivalis and T. forsythensis are higher in test group than control group, and that of T. denticola is higher in control group than in test group. But there were no statistically significant differences between 2 groups. In conclusion, there is no statistically significant difference in prevalence of implant microbiology between 2 groups. But if the number of samples increased, it will be possible to find out statistical significance in prevalence of P. gingivalis. It seems that pocket of adjacent tooth influences prevalence of P. gingivalis. These results mean that improvement of the periodontal condition before implantation is very important.
10.The influence of adjacent tooth to the microbiology of implant sulcus.
Dong Young LEE ; Man Sub LEE ; Young Hyuk KWON ; Joon Bong PARK ; Yeek HERR ; Jong Hyuk CHUNG
The Journal of the Korean Academy of Periodontology 2005;35(3):563-575
The aim of present study is to evaluate the influence of adjacent tooth to the microbiology of clinically healthy implant. Control group included patients who had clinically healthy implant and tooth with healthy periodontium(PD< or =3mm), test group was composed of patients who had clinically healthy implant and tooth with periodontal pocket(PD > 3mm). The criteria of clinically health implant are no pain or discomfort, the restorative suprastructure provide satisfactory fit and function, and the tissue around the fixtures were firm and probing with standard periodontal probe with a rounded tip 0.5mm in diameter resulted in penetration of no more than 5mm when using a force of 0.5N at any location. 38 patients, partially edentulous subjects with endosseous root-form implants were selected. All subjects were medically healthy and had not taken systemic antibiotics and professional plaque control 3 months before sampling. Number of control group is 25(mean age 52+/-13, 26 teeth, 34 implants) and test group is 13(mean age 60+/-13, 13 teeth, 17 implants). All teeth and implants of each patient were examined probing depth(PD), bleeding on probing(BOP), and plaque index(PI), and samples of subgingival plaque were obtained at each site with sterile curet or fine paper points, then the plaque transferred to PBS. Obtained samples were examined for the presence of P. gingivalis, T. forsythensis, and T. denticola by the polymerase chain reaction(PCR). The relationship among clinical parameters and the colonizations by the 3 bacterial species from natural teeth and implants region were analyzed by student t-test. The results of this study were as follows: 1. PD was different in teeth between 2 groups(p<0.05), but the other parameters were not. 2. Statistically significant difference was not found in clinical parameters of implants between 2 groups. 3. All bacterial prevalences of teeth were higher in test group than in control group, and prevalence of T. forsythensis had statistically significant difference between 2 groups(p<0.05). 4. Prevalences of P. gingivalis and T. forsythensis are higher in test group than control group, and that of T. denticola is higher in control group than in test group. But there were no statistically significant differences between 2 groups. In conclusion, there is no statistically significant difference in prevalence of implant microbiology between 2 groups. But if the number of samples increased, it will be possible to find out statistical significance in prevalence of P. gingivalis. It seems that pocket of adjacent tooth influences prevalence of P. gingivalis. These results mean that improvement of the periodontal condition before implantation is very important.