1.Immunohistochemical Study of bcl - 2 Expression in Basal Cell Carcinoma.
Korean Journal of Dermatology 1997;35(2):242-248
BACKGROUND: Basal cell carcinoma(BCC) is the most common tumor of light-skinned people. Most of them are relatively innocuous, but some may cause extensive tissue destruction, and result in death by local infiltration of vital structure or metastatic disease. The bcl-2 is a protooncogene involved in tumorigenesis by blocking apoptosis or programmed cell death. OBJECTIVE: To investigate whether there was a difference in bcl-2 expression in different histopathological types of BCC. Method: We performed immunohistochemical staining by using a monoclonal mouse anti-human bcl-2 oncoprotein in 28 BCC(nodular: 7 cases, micronodular; 3 cases, adenoid; 1 case, infiltrative; 9 cases, and mixed; 8 cases). Result: All 28 BCC expressed bcl-2, and the degree of staining was different according to histopathological types; poor prognostic micronodular(100%) and infiltrative(73.3%) types were relatively strong, and nodular(66.7% ) and adenoid(75.0%) types were moderate. Conclusion . These results suggest that the degree of bcl-2 expression in BCC seems to be dependent to the histopathological types together with clinical behavior.
Adenoids
;
Animals
;
Apoptosis
;
Carcinogenesis
;
Carcinoma, Basal Cell*
;
Cell Death
;
Mice
2.Factors Affecting Complicance in Amblyopia Occlusion Therapy.
Chul Young CHOI ; Hae Ran CHANG
Journal of the Korean Ophthalmological Society 2000;41(12):2633-2637
No Abstract Available.
Amblyopia*
3.A case of recurrent infantile digital fibroma.
Hyun Chul CHOI ; Young Chul KYE ; Chil Whan OH
Korean Journal of Dermatology 1992;30(5):743-746
Recurrent infantile digital fibroma is a peculiar fibrous tumiir that may be single or multiple on fingers and toes of infants and children. We report a 5-year-old gril with recurrent infantile digital fiber oma which was recurrent on the left 5th toe. The lesion had noted at the age of 6 months. and was excised surgically when the child was 3 year old. 2 years after operation, the lesion was recurred in operation site. Microscopically, nodular fibrous mass is attached to flattened,pidermis. The underlying nodule is composed of proliferating fibroblasts surrounded by derse collagenous tissue. We could find eosinophilic inclusion bodies in the cytoplasm of fibro ilast. It was stained pink with H&E, bright red with Massons trichrome and purple with PTAH.
Child
;
Child, Preschool
;
Collagen
;
Cytoplasm
;
Eosinophils
;
Fibroblasts
;
Fibroma*
;
Fingers
;
Humans
;
Inclusion Bodies
;
Infant
;
Toes
4.The efficacy of fetal genetic diagnosis using fetal nucleated erythrocytes in maternal blood.
Jin CHOI ; Young Min CHOI ; Hee Chul SHIN
Korean Journal of Obstetrics and Gynecology 2000;43(11):1939-1946
OBJECTIVE: The conventional methods to determine fetal genetic status, such as amniocentesis or chorionic villi sampling(CVS) have small procedure-related risk of abortion. Recently, several researchers reported that fetal genetic status, such as sex, can be confirmed by fetal nucleated erythrocytes in maternal blood and this method might reduce such risk. Therefore, in this study, we attempted to determine the basic fetal genetic status, sex, with fetal nucleated erythrocytes. METHODS: In twelve pregnant women who undertook amniocentesis or CVS, 20 ml of venous blood was drawn immediately before the procedure and the nucleated erythrocytes were recovered by magnetic activated cell sorting(MACS). After MACS, DNA was extracted from 200 microliter of sample and single nucleated erythrocyte was obtained by additional procedure, immunostaining, and microdissection. After recovery of nucleated erythrocytes by microdissection, nested polymerase chain reaction(PCR) and fluorescent PCR of amelogenin gene were performed to identify the fetal gender. RESULTS: The DNA of enriched erythrocytes after MACS could identify the fetal gender in the 58.3% of the samples by nested PCR. After the recovery of single nucleated erythrocyte by MACS, immunostaining and microdissection, the minute DNA in a single cell could be amplified by primer extension preamplification(PEP), nested PCR, and fluorescent PCR. Fetal genders were correctly identified in 8 out of 12 (66.7 %). CONCLUSION: Through this study, we could conclude that fetal nucleated erythrocytes in maternal blood might be sufficient sample to determine fetal sex. And single cell isolation by microdissection could get the better results than nested PCR after MACS only. However, in spite of the pregnancy of male fetus, female specific bands were obtained after nested PCR of amelogenin in several cells, which might suggest that part of nucleated erythrocytes in maternal blood might be maternal origin. Therefore, to determine fetal genetic condition by nucleated erythrocytes in maternal blood, further improvements of methods to identify the nucleated erythrocytes of fetal origin are needed.
Amelogenin
;
Amniocentesis
;
Cell Separation
;
Chorionic Villi
;
Diagnosis*
;
DNA
;
Erythroblasts*
;
Erythrocytes
;
Female
;
Fetus
;
Humans
;
Male
;
Microdissection
;
Polymerase Chain Reaction
;
Pregnancy
;
Pregnant Women
5.Significance of preoperative abdominal computed tomography in gastric cancer patients.
Young Cheol LEE ; Won Jin CHOI ; Chul Soon CHOI
Journal of the Korean Surgical Society 1992;43(5):653-660
No abstract available.
Humans
;
Stomach Neoplasms*
6.A Clinical Study on the Treatment of Tibial Shaft Fracture in Adult
Joon Young KIM ; Young An CHOI ; Young Chul CHOI ; Young Joe KIM
The Journal of the Korean Orthopaedic Association 1987;22(3):627-637
Sixty four cases of tibial shaft fracture in adult were treated by various methods at Department of Orthopedic Surgery, Koryo General Haspital during 3 years from March 1983 to Feb. 1986. The resuIts of 'treatment of these cases were analysed according to method of treatment, type and level of fracture, and degree of injury, in attempt to seek the most suitable method of treatment. The following results were observed 1. Of 64 cases of tibial shaft fracture, 24 cases were treated by cast immobilization, 16 cases by 0/ R and plate, screw fixation, 17 cases by Ender I-M nailing, 7 cases by Hoffmans ext. fixator. 2. The most common site of fracture was middle 1/3 of the shaft (62.5%). 3. On the union time of fracture, I-M nailing by use of Ender nail was more rapid than other methods of treatment. (average: 16.2 Wks. POD) 4. On the time of weight bearing, earlier weight bearing was possible by Ender I-M nailing than other method of treatment. 5. On the complications of fracture, fewer complications were developed after Ender I-M nailing than other method of treatment. 6. According to Modified Ellis classification, more rapid union was obtained by C/R and cast imm-obilization in the case of Minor degree, no significant difference among each group in the case of Moderate degree.
Adult
;
Classification
;
Clinical Study
;
Humans
;
Immobilization
;
Methods
;
Orthopedics
;
Tibia
;
Weight-Bearing
7.Dorsal Nerve Somatosensory Evoked Potential Test for Localizing the Lesion in Neurogenic Erectile Dysfunction.
Won Jae YANG ; Young Deuk CHOI ; Young Chul CHOI ; Sang Yol MAH ; Hyung Ki CHOI
Korean Journal of Urology 2000;41(5):645-649
No abstract available.
Erectile Dysfunction*
;
Evoked Potentials, Somatosensory*
;
Male
8.Airway Obstrauction during Endotracheal Anestheaia - Case report.
Young Chul LEE ; Ki Young LEE ; Sung Chul CHOI
Korean Journal of Anesthesiology 1987;20(3):432-434
Acute airway obstruction during endotracheal anesthesia is embarrassing and critical situation which requires early diagnosis and immediate management. The authors experienced extremely high inspiratory resistance and declared expiration time during endotracheal anesthesia for right pneumonectemy of lung cancer in the left decubitus position. A foreign material was suspected to be dislodged in the trachea distal to the endotrac-heal tube. But the surgical procedure did not allow to change the tube for examination. Ventilation was managed by rotating and moving the endotracheal tube frequently. After extubation, the patient expectorated a polypoid mass and the cause of the obstru-ction was proved to be a result of the detached and dislodged tumor mass of the lung, in diameter 3X1.5 cm.
Airway Obstruction
;
Anesthesia
;
Early Diagnosis
;
Humans
;
Lung
;
Lung Neoplasms
;
Trachea
;
Ventilation
9.Cutaneous Chylous Reflux.
Dong Sik BANG ; Woo Chul SHIM ; Young Sik CHOI
Korean Journal of Dermatology 1989;27(6):704-708
We describe a case of cutaneous chylous reflux, which is thought to be a kind of primary lymphedema, in a 19-year-old male who had suffered from multiple vesiculobullae and intermittent swelling on scrotum for about 6 years. Histopathologic findings showed multiple dilated lymphatic vascular channels in papillary dermis.
Dermis
;
Humans
;
Lymphedema
;
Male
;
Scrotum
;
Young Adult
10.The Mechanism of Blood Flow Generation during Closed Chest Cardiac Massage: Transesophageal Echocardiographic Monitoring.
Young Hi LEE ; In Chul CHOI ; Myung Won CHO
Korean Journal of Anesthesiology 1997;33(5):957-961
The mechanism of forward blood flow during closed chest cardiac massage remains controversial. Two theories have been suggested: the cardiac pump theory and the thoracic pump theory. Case report is presented to illustrate the use of transesophageal echocardiography during cardiopulmonary resuscitation. The findings included right and left ventricular compression, closure of the mitral valve during compression, opening of the mitral valve during the release phase, and atrioventricular regurgitation during compression, indicating a positive ventricular-to-atrial pressure gradient. These findings suggest that direct cardiac compression was the predominant mechanism of forward blood flow during cardiopulmonary resuscitation in this patient. Transesophageal echocardiography offers a new approach for study of the flows and cardiac morphologic features during chest compressions in humans. An understanding of the actual mechanisms involved is necessary if improved cardiopulmonary resuscitative techniques are to be rationally developed for enhancing the outcome of resuscitation.
Cardiopulmonary Resuscitation
;
Echocardiography*
;
Echocardiography, Transesophageal
;
Heart Massage*
;
Humans
;
Mitral Valve
;
Resuscitation
;
Thorax*