Objective To investigate the effect and significance of minimally invasive removal of intracranial hematoma on the blood-brain barrier in patients with intracerebral hemorrhage (ICH).Methods Clinical data of 20 patients with ICH were randomly selected and received minimally invasive surgery (operative group) and 20 patients with ICH received expectant treatment therapy (expectant treatment group) were analyzed retrospectively,and 20 healthy people were randomly collected for control group.The blood-brain barrier (BBB) and serum myelin basic protein (MBP) concentration were analyzed.Results The BBB index of the iperative group was 0.0075 ± 0.0007,the expectant treatment group was 0.0083 ± 0.0006 and the control group was 0.0068 ± 0.0004.The BBB index in the operative group was significantly lower than that in the expectant treatment group (P ＜ 0.05),and the BBB index in these two groups was significantly higher than that in the control group (P＜ 0.01).The serum MBP concentration in the operative group was (3.29 ± 1.55) μg/L,the expectant treatment group was (6.55 ± 3.78) μg/L,and the control group was (1.12 ± 0.48) μ g/L.The serum MBP concentration in the operative group was significantly lower than that in the expectant treatment group (P ＜ 0.05),and these two groups were significantly higher than the control group (P ＜ 0.01).Conclusions The permeability of BBB in patients with ICH are increased,the BBB index and the serum MBP concentration in patients with ICH are increased,minimally invasive surgery can reduce the lesion of cytotoxicity to BBB and cerebral edema.

The interdisciplinary nature of health economics brings certain difficulty to the teaching of this course, so it is very important to rationally design and use case teaching. According to the characteristics of the course, this paper put forward design ideas from the guiding ideology, selec-tion, categories and forms. This paper argued that teacher should play a leading role and create a good learning environment and grasp four parts of the implementation.

Chitin is one of the most important component in fungal cell wall.Biosynthesis of chitin is a complex processes and needs several chitin synthase isoenzymes.The knowledge of structure,function and regulation of chitin synthases is mainly derived from the study of Saccharomyces cerevisiae.In contrast with the 3 chitin synthases in S.cerevisiae,7 were found in most filamentous fungi.In this review the classification and function of chitin synthases are summerized,and progress in the studies on chitin synthases of filamentous fungi which are of theoretical or medical or agricultural importance,including Aspergillus nidulans,Aspergillus fumigatus and Ustilago maydis are emphasized.Recent ad-vance of research on chitin synthase as antifungal target is also discussed.

The study is to explore the effect of paeoniflorin on the level of glucocorticoid receptor, including glucocorticoid receptor-alpha (GCRalpha) and glucocorticoid receptor-beta (GCRbeta), of peripheral blood mononuclear cells (PBMCs) in rats of collagen-induced arthritis (CIA). CIA is induced in Wistar rats by an intradermal injection of bovine type II collagen emulsified with complete adjuvant. From the 14th day after primary immunization, the CIA rats were intragastrically administered paeoniflorin 25, 50 and 100 mg x kg(-1) or triptolde 20 microg x kg(-1) or paeoniflorin 50 mg x kg(-1) + RU486 15 mg x kg(-1), once a day, for 28 consecutive days. After administration, apart from PF + RU486 group all experimental rats were took blood by removalling eyeball, then separated PBMCs. The level of GCRalpha, GCRbeta in PBMCs were examined by ELISA, and the mRNA expression of GCRalpha, GCRbeta was detected by RT-PCR. All rats were sacrificed and took the joint with no immunization. The expression of IL-1beta, NF-kappaB p65, TNF-alpha, PGE2 of synovial tissue was detected by immunohistochemistry. Paeoniflorin was able to inhibit the expression of IL-1beta, NF-kappaB p65, TNF-alpha, PGE2 of synovial tissue in CIA rats. While RU486, glucocorticoid receptor's blocker, could weaken the fuction of paeoniflorin. Meanwhile, paeoniflorin obviously induced the expression of GCRalpha and GCRalpha mRNA, while obviously inhibited the expression of GCRbeta and GCRbeta mRNA. These results indicat paeoniflorine suppresses inflammatory mediator production may be relating with it regulating GCR in PBMCs of CIA rats.
Animals ; Arthritis ; chemically induced ; drug therapy ; genetics ; metabolism ; Cattle ; Collagen ; adverse effects ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Glucosides ; administration & dosage ; Humans ; Leukocytes, Mononuclear ; drug effects ; metabolism ; Male ; Monoterpenes ; administration & dosage ; Rats ; Rats, Wistar ; Receptors, Glucocorticoid ; genetics ; metabolism

AIM: To study the correlation between the drug effects and serum HPLC fingerprint from active compositions of Radix paeoniae alba and Radix et Rhizowa Glycyrrhizae. METHODS: Orthogonal design of L_9(3~4) of the different combinations of three active compositions of paeoniflorin,glycyrrhiza flavoues and Glycyrrhizic acid were prepared,antalgic pharmacological experiments of mice were carried out.The fingerprints of the serum were tested by HPLC method and different chromatographic peaks were obtained.Correlation analysis was applied to interelationship between the area of each peaks and the pharmacologic data. RESULTS: Correlation coefficients were different between pear areas and analgetic coffec. CONCLUSION: This study provides a significant try of investigating the compatibility of composite herbal medicines.

AIM:To explain the drug-release behavior of paeoniflorin from paeonia glycosides sustained-releasing pellets according to different coating material. METHODS: HPLC method was used to gain the fingerprint of pellets,paeoniflorin was sellected as marker to calculate the linear equation concerning peak area,and acquire relative drug-release. RESULTS: The releasing process of paeoniflorin and other constituents from paeonia glycosides fit in with peppas and Huguchi equation. CONCLUSION: The chromatographic peaks stand for paeonia glycosides can well elucidate the releasing behavior in vitro of paeonia glycosides sustained-releasing pellet.

AIM:To evaluate the efficacy of Wuling Capsule(xylaria) in treating senile systolic hypertension. METHODS: Sixty-four patients with senile systolic hypertension were randomly divided into two groups.Both the treated group and the control group were treated with anti-hypertensive for 4 weeks,but the treated group received Wuling Capsule.Therapentic effects were evaluated with HAMA.Noninvasive resting blood pressure was taken every day after treatment,and dynamic blood pressure before and after treatment. RESULTS: HAMA in both groups decreased somewhat but blood pressure in the treated group was lower than that in the control group,remission rate of the treated group was greater than that in the control group.Treatment period of the treated group was shorter than that of the control group. CONCLUSION: Wuling Capsule can relief anxiety in senile hypertension patients,and it can rise the control rate of blood pressure,and also shorten the treatment period.

Through focusing on widely concerned maternal death incident in Peking University Third Hospital,something can be discovered:for the upper hand in doctor-patient conflicts,they start to have a scramble for discourse power based on information dissemination of social media.On one hand,the evolution of social media builds a bridge for interactions and connections between doctors and patients.It contributes to rebuilding professional authority for doctors and increasing objectivity of information dissemination,On the other hand,information dissemination leads the new scramble for discourse power.It causes some problems:self-reflection decline of doctora,truth diiution about incident and even some moral and ethical risks both patients and doctors.Media tactics of doctors are Proposed to regulate and improve the scramble for discourse power in the social media context from two big aspects,namely establishing matrix platform and reducing propagation loss about social media.

This paper gave a dynamic analysis on the health resource allocation and utilization of Chongqing since it became a municipality,found out the extrusive problems existing in the health resource allocation and put forward some corresponding proposals,hoping to benefit the health administration on constituting regional health development planning.

Objective To investigate the distribution and duration of abnormal blood glucose level in SGA infants. Methods Blood glucose level was systemicly monitored in 37 SGA infants for (133?115) h(range 16～524 h, median 93 h), who were non NICU hospitalized and no feeding or IV infusion when admitted at (2 6?2 4) h (range 0.25~10.0 h, median 2.0 h) of age. Results During monitoring period, there was hypoglycemia in 19 cases (51%),hyperglycemia in 2 cases (5%), and both hypo and hyperglycemia in 3 cases (8%). The median of last time abnormal glucose level appeared was 10 hr after birth, its 95% confidence interval was 5~55hr after birth. Multiple regression analysis shown delivery models bad relationship with glucose level at admition (p= 0.013). The blood glucose level was higher in vaginal delivery babies. The lower the birth weight (BW)was, the longer the duration of abnormal glucose level lasted ( O