Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.

The initiation and development of diabetic chronic complications is associated not only with the level of blood glucose,but also with the glucose fluctuation.The damage in large artery and microvessel may be caused by glucose fluctuation.There are two causes which induce glucose fluctuation in clinical:one is postprandial hyperglyceamia with not well controlled,the other is hypoglycemia causes by unsuitable therapy.A lot of effective new types of substances were applicated in clinical,such as ?-glucosidase inhibitor,glinide-insulin secretagogus,fast-acting and long-acting insulin analogues.With these new powerful tools,there is possibility to treat diabetes more correctly and safely,and control the blood glucose in a stable and good level.

The incidence of malnutrition among patients with chronic kidney disease (CKD) constantly increases despite the development of health care and dialysis techniques.New nutritional targets for CKD patients upon maintenance treatment have been developed.Before dialysis,evidences have shown that a long-term nutritional care plan,with a control of protein intake,can effectively correct metabolic disorders including proteinuria and acidosis.During the maintenance dialysis,the optimal protein and energy intakes have been recently challenged.The new classification of nutritional disorders in CKD patients may help the easily identification of protein-energy wasting.

Arthroplasty, Replacement, Hip ; trends ; China ; Hip Prosthesis ; Humans ; Prosthesis Failure

Humans ; Osteotomy ; adverse effects ; methods ; Scoliosis ; surgery

Humans ; Minimally Invasive Surgical Procedures ; Spine ; surgery

BACKGROUND:A variety of stem cells as seed cells for cartilage tissue engineering have been extensively studied.
OBJECTIVE:To analyze the biological characteristics of different kinds of stem cells and their application in cartilage tissue engineering.
METHODS:By retrieving recent studies on sources of stem cells and influential factors in cartilage tissue engineering, the authors focused on optimization of harvesting stem cells from different sources in bone tissue engineering, problems, and factors that affect cellgrowth, differentiation, reproduction and metabolism, which provide a theoretical basis for tissue-engineered cartilage repair.
RESULTS AND CONCLUSION:A large number of experimental studies have confirmed that exogenous stem cells can be used as seed cells for cartilage tissue engineering. Influential factors relative to cartilage tissue engineering construction using seed cells include growth factors, cellscaffold, cellseeding density, oxygen concentration, stress and micro-gravity. However, tissue-engineered cartilage is stil in the stage of laboratory research, and stem cells for cartilage tissue engineering involves many issues, such as stem celltransformation, in vitro culture of stem cells, how to prolong the survival of cells, reduce cellantigenicity and enhance the host’s immune tolerance, which are yet to be resolved.

Medical words distinguish medical paper from any other papers,and the right choice of medical words plays an important role in conveying medical content.However,with respect to the whole text,a larger number of words are still nonmedical ones,to which great importance should also be attached.In the writing of a medical paper,three principles of exactness,conciseness,and readability in selecting nonmedical words should be followed to make the paper more internationally accepted.

Many transcription factors, kinases and phosphorylase regulate the expression and activity of stuthmin. The abnormality of stathmin expression or its activity is closely involved in lots of pathological or phys-iological events such as cell proliferation, development of tumor. Many kinds of malignant tumors have high ex-pression of stathmin, and inhibiting the expression of stathmin protein is a new biotherapy target in tumor geneth-erapy. Moreover, stathmin protein can influence curative effect of some chemotherapeutics ,which target microtu-bular. This has some significance with regard to the guidance of clinical medication.

Objective:To construct a eukaryotic vector containing human TACI-linker-BR3-IgGFc double receptor fusion gene and to investigate its expression in COS-7 cells.Methods: The standard cloning technology was employed to construct the(title) vector,which was then used to transfect COS-7 cells by lipofectamine 2000.The expression of target protein and their interaction with BAFF and APRIL protein were examined by Western blot,ELISA,and immunohistochemistry methods.Results: The expression of the fusion protein was observed in transiently transfected COS-7 cells, with the transfection efficiency being 48%.The fusion protein was detected in the supernatant.It suggested that the fusion protein interacted well with BAFF and(APRIL) proteins;the IgGFc tag did not influence the natural assembly and the biological activity of fusion proteins.Conclusion: A vector containing TACI-linker-BR3 double receptor and IgGFc fusion gene has been successfully constructed and the(subsequent) fusion protein is bioactive,which paves a way for further research on the pathogenesis of autoimmune diseases and biotherapy strategy.