ObjectiveTo develop the military acute stress scale and examine the reliability and validity of this scale.MethodsBased upon the previous acute stress researches and the rule of psychometrics,the military acute stress scale was compiled according to open-ended inventory survey.A total of 630 personnel were chosen by random cluster sampling,and tested with this scale,50 of them were tested by Chinese Military psychosomatic health Scale simultaneously,and 50 of them were retested after two week.The reliability and validity were examined by exploratory factor analysis,confirmatory factor analysis and correlation analysis.ResultsThe military acute stress scale was comprised of 9 factors and one lie sub-scale ; Main fit indexes of model were the following CMIN =1219.476,DF =593,P =0.000,CMIN/DF =2.056,IFI =0.927,TLI =0.902,CFI =0.924,RMSEA =0.059; The conrelation coefficients between subscales and subscales were 0.284-0.572.The correlation coefficients between subscales and total scale were 0.700 ～ 0.846 (P ＜ 0.05 or 0.01 ) ; The correlation coefficients was significant between this scale and Chinese military psychosomatic health scale.The correlation coefficients were 0.338-0.962 (P ＜ 0.01 ).The test-retest correlation coefficients of total scale and subscales were 0.379-0.909 (P ＜ 0.01 ).The Cronbaeh's α coefficients were 0.606-0.926 ; and the split-half correlation coefficients were 0.466-0.855.ConclusionThe military acute stress scale has acceptable psychometrics properties on reliability and validity.

Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

Venous thrombotic disease is a serious disease,which impact on health and life-threatening.Pulmonary embolism and deep-vein thrombosis are the two components of a single disease called venous thromboembolism in obstetrics and gynecology.but it can be difficult to diagnose because clinical symptoms and signs are non-specific or absent in early venous thrombus embolism (VTE).It has great value that how to use the most economic,simple,efficient method for screened high-risk groups,timely and accurate laboratory diagnosis of VTE.

Objective To observe the effect and safety of different doses of sufentanil plus subarachnoid block during labor. Methods One hundred parturients (single birth) were divided into 5groups by random digits table with 20 cases in each, which sufentanil 3,4,5,6,7 μ g in group A, B, C, D, E plus ropivacaine 3 mg were injected respectively into subarachnoid space. The onset and duration of subarachnoid analgesia were observed; visual analogue scale (VAS) were recorded respectively before injection and 5,15,30,60 min after injection; the vital sign,labor stage,labor type,adverse reactions of parturients,fetal heart rate (FHR) and the Apgar score at 1,5 min of neonate were recorded respectively.Results The onset of subarachnoid analgesia in group A was higher than that in group B,C, D,E;the duration of subarachnoid analgesia in group A,B was shorter than that in group C,D,E.VAS declined gradually 5,15,30,60 min after injection compared with before injection in 5 groups (P ＜ 0.01 );VAS in group B, C, D, E was lower than that in group A at 5 min after injection (P ＜ 0.05 ), and VAS in group C, D, E was lower than that in group A,B at 60 min after injection (P＜ 0.05). There was no significant difference in vital sign,labor stage,labor type,FHR and the Apgar score of neonate among 5 groups (P ＞0.05). The occurrence of itch of skin and nausea in group D,E [25%(5/20), 15%(3/20) in group D,and 40%(8/20),20%(4/20) in group E] was higher than that in group A, B, C [5%(1/20), 0 in group A, 10%(2/20), 0 in group B, 10% (2/20), 0 in group C] (P ＜ 0.05 ). Conclusion Subarachnoid block (sufentanil 4-6 μ g +ropivacaine 3 mg) can offer safety and efficacy of labor analgesia; sufentanil 5 μg is the best dose with subarachnoid block during labor at present.

Based on the analysis of the medical students' humanities quality and current college English teaching,this paper explored four strategies including pre-class lead-in activity,making full use of current teaching materials,reforming present college English teaching method and developing extracur-ricular practical activities in order to foster humanities quality in college English teaching and to improve medical students' humanities quality.

Fifty-six middle and aged post-herpetic neuralgia patients were treated with Jiaji (Ex- B 2) of the correspondingly affected segments plus Ashi points. The major points were treated with even reinforcing-reducing manipulation and the auxiliary points were treated by puncturing subcutaneously around the skin lesion with reducing manipulation. Afterward the three-edged needle was used to insert into the tenderness spots rapidly and remove immediately for 3-5 consecutive times around the spot plus flash-fire cupping. The result showed recovery in 36 cases; marked effect in 17 cases; no effect in 3 cases and the total effective rate was 94.6%.

Necrotizing enterocolitis(NEC) is a common and devastating gastrointestinal disease that occurs predominantly in premature infants.Despite various advances in management,the mortality of this disease remains high.During the last decade,studies have shown that heparin-binding epidermal growth factor-like growth factor (HB-EGF),can protect intestinal epithelial cells from various forms of injury in vitro.Furthermore,studies using both an intestinal ischemia-reperfusion injury model in adult rats and neonatal rat model of NEC,have shown that HB-EGF can protect the intestines from injury.Present studies provide a new research direction in the prevention and treatment.

Objective To explore the influence of electro-acupuncture combined with hyperbaric oxygen on cognitive ability and plasma homocysteine(Hcy) in patients with dementia after cerebral infarction.Methods 68 patients with dementia after cerebral infarction were randomly divided into the control group (34 cases) and the observation group(34 cases).The patients in the control group were treated through conventional treatment,and the patients in the observation group were treated through electric stimulation plus hyperbaric oxygen,one time every day,15 times was a course of treatment.The intelligence was evaluated and the level of Hcy was detected after treatment.Results Before treatment,the MMSE score and the plasma Hcy level of the observation group was (19.01 ±6.92) points,(26.84 ± 9.06) μmol/L,respectively,those of the control group was (18.76 ± 6.71) points,(26.14 ±8.79) μmol/L,respectively.After treatment,the MMSE score and the plasma Hcy level of the observation group was (25.77 ± 8.21) points,(15.15 ± 5.24) μmol/L,respectively,those of the control group was (20.18 ± 7.52) points,(21.32 ± 6.53) μmol/L,respectively.After treatment,the MMSE score of the observation group was significantly increased (t =3.6710,P ＜ 0.05),and there was no significant change in the control group (t =0.5472,P ＞ 0.05).The plasma Hcy level was significantly reduced after treatment in both two groups (t =2.0345,P ＜ 0.01,t =2.5667,P ＜0.05),and that in the observation group was siginificantly lower than the control group (t =4.2970,P ＜ 0.05).Conclusion Electric stimulation combined with hyperbaric oxygen can improve cognitive ability and decrease plasma Hcy level in patients with dementia after cerebral infarction.

Objective:To investigate the expression of five drug resistance genes in hepato cellular cancer,lung cancer,stomach cancer,colorectal cancer,ovarian cancer,breast cancer tissue. Methods:The expression of P-gp,MRP,LRP,GST-?,Topo-Ⅱ in 120 cases of malignant tumors were examined by immunohistochemistry stain. Results: 1. The expression of P-gp,MRP,LRP,GST-? in the 6 common cancers were relatively high,while Topo-Ⅱ was relatively low. 2. P-gp,MRP,LRP,GST-?,Topo-Ⅱ were all high expressed in hepatocellular cancer. 3. The coexpression rate of two and more genes was 95.83%,the coexpression rate of five genes was 21.67%. Conclusion:P-gp,MRP,LRP,GST-?,Topo-Ⅱ are associated with multidrug resistance in different degree,which can be regarded as valuable indicators for the decision of individual chemotherapeutic program.

Objective:To identify the best method for isolating and culturing mature rat adipocytes in vitro.Methods:Three different primary culture methods,i.e.organ culture,ceiling culture,and modified ceiling culture were used in the present study.Mature Sprague-Dawley rat adipocytes were cultured in vitro.The cells were observed under microscope and oil red O staining to compare the distinction of cells with different culture methods in the same condition.Rusults:Adipocytes were successfully cultured by the three primary cell culture methods.Cells cultured were highly homogenous.Modified ceiling culture method was the best in terms of the efficiency of culturing and cell morphology.Conclusion:Modified ceiling culture,is a long-term stable primary culture method for mature rat adipocyte,providing a good technique platform for the studies related to adipocytes.