Biomarkers ; Hepatitis B ; therapy ; virology ; Hepatitis B Surface Antigens ; Hepatitis B virus ; Humans

Child ; Cranial Nerve Injuries ; drug therapy ; etiology ; Diagnosis, Differential ; Female ; Glossopharyngeal Nerve ; pathology ; Humans ; Malaria, Cerebral ; complications ; diagnosis ; drug therapy ; Vagus Nerve ; pathology

Objective To investigate the clinical effect of sitagliptin combined with metformin in the treatment of sulfonylurea-deficient elderly patients with diabetes mellitus.Methods Sixty patients with diabetes mellitus were enrolled in our hospital from January 2013 to December 2016.The patients were divided into observation group(30 cases) and control group(30 cases) according to the random number method.The patients in the observation group were treated with sitagliptin and metformin.The patients in the control group were treated with gemcitabine, and the clinical treatment effect, fasting blood glucose, postprandial blood glucose, glycosylated hemoglobin, fasting C peptide and postprandial 2hC peptide Level of statistical comparison.Results The clinical effect of the observation group was significantly higher than that of the control group.Before and after treatment, the fasting blood glucose, 2h postprandial blood glucose, glycosylated hemoglobin, fasting C peptide and postprandial 2hC peptide level were not significantly different After fasting blood glucose, 2h postprandial blood glucose and glycosylated hemoglobin levels were significantly lower than before treatment, after treatment of fasting C peptide and postprandial 2hC peptide levels were significantly higher than before treatment.Conclusion The clinical efficacy of serotretine combined with metformin in the treatment of elderly patients with diabetes mellitus is similar to that of Yuxing, and the combination is more convenient and more suitable for clinical treatment.It is worthy to be popularized and applied in clinical practice.

Objective: Hitherto there has been no report on the changes of microvessels and the expression of angiogenic factors after photodynamic therapy(PDT) of liver carcinoma.The purpose of this study was to investigate the effect of PDT on the microvessels and the expression of vascular endothelial growth factors(VEGF) in tumor cells. Methods: The mice models subcutaneously transplanted with Heps liver carcinoma were randomly divided into a PDT group(n= 28) and a control group(n= 28).The dynamic changes of the microvessels and VEGF expression in the tumor cells were observed at 2,6,24 and 72 h after PDT by H-E and immunohistochemical staining.Results: Progressive changes were seen in the tumor microvessels at the four time points after PDT,including dilatation,blood stasis,hypostasis,hemorrhage,and angiolysis.Compared with the control group,the microvessel density of the tumors was reduced significantly(P

Hepatolenticular Degeneration ; Humans ; Nervous System Diseases

Objective To observe the effect of Ganshu Granule on different models of fatty liver in rats. Methods Rats with acute fatty liver induced by alcohol and carbon tetrachloride, rats with chronic fatty liver induced by alcohol and high-fat diet, and rats with acute fatty liver induced by ethionine were used, and the effects of different doses of Ganshu Granule on serum lipid metabolism, intrahepatic lipid metabolism and histopathological changes in liver were observed. Results In fatty liver rats induced by alcohol and carbon tetrachloride, Ganshu Granule could reduce the level of total cholesterol (TC), triglycerides (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in serum and could decrease total fat and glycogen(Gn) in liver obviously. In fatty liver rats induced by alcohol and high-fat diet, Ganshu Granule could significantly decrease the level of TC, TG, ALP, LDL and ALT in serum and could reduce the content of total fat, TG and Gn in liver obviously. In fatty liver rats induced by ethionine, Ganshu Granule could decrease the level of TC, TG and ALT in serum, raise the level of HDL in serum and could decrease the content of total fat, TG and Gn in liver obviously. The histopathologic results showed the degree of hepatic steatosis and hepatocellular necrosis were lessened in all of the treated groups. Conclusion Ganshu Granule is effective in preventing and treating fatty liver.

Objective To know the ability of degrading oxalate of three species of Lactic acid bacterium and their mixing bacteria coming from yoghurt in vivo culture broth. Methods Identifying the species of Lactic acid bacteria obtained from 6 brands of yoghurt.Three species of bacterium and their mixture was cultured separately for 72 h in MRS broth which contained three different concentration of ammonium oxalate,and the broth without inoculum of bacteria was prepared as a control.The concentration of ammonium oxalate in culture were detected. Results Three species of Lactic acid bacteria (L.bulgaria,L.acidophilus and L.thermophilus) were detected in every band of yoghurt.After 72 h incubation,the concentration of oxalate in all the broth containing bacteria was lower than that in the control ones.L.acidophilus showed the best degrading activity,and in the presence of ammonium oxalate 5 mmol/L,the 11.0% of oxalate was degraded by the bacteria.The higher concentration of oxalate was,the more the amount of oxalate degraded was,but the lower the percent of degrading was. Conclusions All the species coming from yoghurt have the ablitiy of oxalate degrading which become greater as the increasing of the concentration of oxalate in culture.

Objective To study the clinical effects of endovascular stent-graft repair for thoracic aortic aneurysm(TDA).Methods The clinical records of 32 patients with thoracic aorta dissecting aneurysm,including 26 men and 6 women,who underwent endovascular repair were retrospectively analyzed.The characteristics,location,and anatomic condition of dissecting aneurysm were evaluated using CTA before operation.Under general or local anesthesia,32patients underwent endovascular repair under dynamic DSA.For those patient,8 Talent,22 Yuhengjia(Beijing) and 2 Microinvasive(Shanghai) stent grafts were used.Results Among them 32 patients the distance between entry tear and left subclavian artery was

Objective To discuss the clinical value of modified laparoscopic lithotomy of common bile duct.Methods Clinical data of 78 patients over the age of 60 with gallstone and choledocholithiasis who underwent modified laparoscopic lithotomy of common bile duct in the past four years were analysed.Results All operations were successful.T-tubes were withdrawn 4 weeks after operation.Of the 78 cases,77 cases hat no complications and only one case had a small incision infection.All patients recovered without complications after with drawal of T-tube.Conclusions Modified laparoscopic lithotomy of common bile duct can allow incision and suture of common bile duct and placement of T-tube under direct vision,and achieves excellent therapeatic results in the treatment of common duct stones,especially in elderly patients who cannot tolerate a long period of pneumoperitoneum.

At present, mesenchymal stem cell treatment mainly applies in local and systematic transplantation, gene therapy combining stem cells, as well as tissue engineering. Based on mesenchymal stem cells are easily separated, cultured, have potentials of fast proliferation, easy exogenous gene's induction and expression, and can maintain multi-differentiation potential throughout long-term culture in vitro, and the quite stable genetic background. Mesenchymal stem cells have widely application prospect in tissue engineering of ophthalmology. At present, domestic and foreign researches have had massive evidences that mesenchymal stem cells can differentiate into cornea and retina cells. The application of mesenchymal stem cells in the anterior eye is usually by dint of a kind of carrier, such as amniotic membrane, fibrin gel membrane and so on. Moreover, the temperature sensitive culture dish has made the non-carrier transplantation of ocular surface stem cells. The application of mesenchymal stem cells in the posterior eye mostly concentrates in seeking for the feasible treatment of retinitis pigmentosa. Researches are mature on mesenchymal stem cells as seed cells acting on damaged tissue in ocular repairs and regeneration. But its oncogenicity and the security are waiting for further solving.