1.Identification of eplication-ompetent Dimeric Human Hepatitis B Viral Using PCR Screening Technique.
The Korean Journal of Hepatology 1999;5(3):184-189
BACKGROUND/AIMS: Transfection of the hepatitis B virus (HBV) genome requires the cloning of tandem HBV sequences inserted into a plasmid vector, which is usually screened for by the restriction enzyme digestion of plasmid minipreparation from at least a dozen of bacterial colonies. The aim of this study was to develop a simple alternative screening method for bacterial colonies harbouring tandem HBV sequences by a PCR. METHODS: A set of polymerase chain reaction (PCR) primer was designed to detect the bacterial colonies harbouring "head to tail" dimeric HBV DNA. PCR which amplifies the head to tail junction site of two tandem HBV molecules was performed. RESULTS: PCR products with appropriate size (1.2kb) were obtained. The accurate detection by PCR screening technique was confirmed by enzyme digestion. CONCLUSIONS: These results suggest that PCR screening technique is a simple and rapid method for the identification of bacterial colonies containing tandem HBV sequences.
Clone Cells
;
Cloning, Organism
;
Digestion
;
DNA
;
Genome
;
Head
;
Hepatitis B virus
;
Hepatitis B*
;
Hepatitis*
;
Humans*
;
Mass Screening*
;
Plasmids
;
Polymerase Chain Reaction*
;
Transfection
2.Anesthetic Management for Selective Dorsal Rhizotomy.
Won Hyung LEE ; Jeong Ok CHO ; Hyun Suk CHO
Korean Journal of Anesthesiology 1998;35(5):939-945
Background: Cerebral palsy is due to static encephalopathy during perinatal period. Selective dorsal rhizotomy (SDR) involves selective division of posterior nerve roots to reduce spasticity and improve function in children with spastic cerebral palsy. Anesthesia during SDR must preserve muscle contraction in response to direct electrical stimulation of the dorsal nerve roots. We did this study to get the better management of anesthesia for SDR. Methods: Anesthetic records were reviewed for 16 patients who underwent SDR during January 1996 to August 1997. Demographic data; anesthetic drugs and doses; changes of vital signs and end tidal CO2; dorsal root stimulation; postoperative pain control were analysed. Results: The mean age of patients was 4.9+/-1.7 years old. The mean weight was 16.3+/-4.0 kg. The under 1 MAC concentration of isoflurane and 2~3 mcg/kg/hr fentanyl did not interfere with electrophysiologic monitoring. Esophageal temperature was increased significantly during electrical stimulation of dorsal roots. End tidal CO2 concentration had a tendency to increase after electrical stimulation too. Direct installation of 10~15 mcg/kg intrathecal morphine prior to dural closure, and postoperative 0.5 mcg/kg/hr fentanyl had a good postoperative analgesia without complication. Conclusions: Isoflurane and fentanyl during anesthesia, and intrathecal morphine with continuous infusion of fentany postoperatively are suggested a good anesthetic method for SDR.
Analgesia
;
Anesthesia
;
Anesthetics
;
Cerebral Palsy
;
Child
;
Electric Stimulation
;
Fentanyl
;
Humans
;
Isoflurane
;
Morphine
;
Muscle Contraction
;
Muscle Spasticity
;
Pain, Postoperative
;
Rhizotomy*
;
Spinal Nerve Roots
;
Vital Signs
3.Hairy Blue Nevus.
Sang Hyun CHO ; Baik Kee CHO ; Sang In SHIM ; Won HOUH
Annals of Dermatology 1989;1(2):123-125
A 2-month-old female infant presented with a bluish-black, flat, hairy patch, 1.2cm in diameter, on the posterior aspect of her neck since birth. Histologic sections from the lesion showed numerous diffusely scattered dermal melanocytes throughout the dermis. The histopathologic and clinical findings were not fully oonsistent with any other known dermal melanocytosis. The diagnosis of “hairy blue nevus” was proposed for this unique lesion.
Dermis
;
Diagnosis
;
Female
;
Humans
;
Infant
;
Melanocytes
;
Neck
;
Nevus, Blue*
;
Parturition
4.Detection of human cytomegalovirus DNA polymerase gene by polymerase chain reaction.
Hyun Chul KIM ; Sung Bae PARK ; Won Hyun CHO ; Won Ki BAEK ; Min Ho SUH
Journal of the Korean Society for Microbiology 1992;27(2):181-188
No abstract available.
Cytomegalovirus*
;
DNA*
;
Humans*
;
Polymerase Chain Reaction*
5.the Effects of Mono-Poly on The Soft Denture Liners.
Ji Hyun HEO ; Tai Ho JIN ; Hye Won CHO
The Journal of Korean Academy of Prosthodontics 2000;38(4):484-491
Soft denture liners or conditioners may be valuable therapeutic materials. The life of these liners is short and varies, but it can be extended by the use of a mono-poly(polymethyl methacrylate coating material). This study determined whether coating three temporary soft denture liners with mono-poly affected the resiliency of soft liners, and evaluated the ability of mono-poly to prevent water absorption and alcohol loss from the underlying soft liners. 20x12mm cylindrical specimens of Coe-Soft, Soft-Liner, Coe-Comfort soft lining materials were made and divided into two groups of mono-poly uncoated(control) and mono-poly coated specimens. Specimens were immersed in water and compressed on an instron universal testing machine and weighted at initial, 24 hours, 1 week, 2 weeks, and 4 weeks. resiliency was determined by measuring the energy absorbed by the soft liners when stressed to a specific yield point. Mono-poly coating significantly increased the resiliency of the Soft-Liner, but had no effect on the other soft liners. Among the 3 soft liners, Soft-Liner showed the hightest resiliency and modulus of elasticity. The weight loss in Soft-Liner was the least among the 3 liners, and the weight loss in Coe-Soft was decreased by monopoly coating.
Absorption
;
Denture Liners*
;
Dentures*
;
Elastic Modulus
;
Water
;
Weight Loss
6.Clinical experiences of thumb reconstruction.
Joong Won SONG ; Joon Hyun CHO ; Jin Sung KANG
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1993;20(5):1151-1162
No abstract available.
Thumb*
7.Patterns of Vascular Invasion of Intrahepatic Peripheral Cholangiocarcinoma Examined with Angiography and Angiographic CT.
Jae Chun CHANG ; Hyun Cheol CHO ; Won Kyu PARK
Journal of the Korean Radiological Society 1995;32(1):145-152
PURPOSE: To evaluate the radiological patterns of vascular invasion in peripheral cholangiocarcinomas. MATERIALS AND METHODS: Hepatic arteriography and portography in 20 cases with cholangiocarcinoma including 12 cases with anglographic CT were retrospectively analized. RESULTS: The arteriography showed no arterioportal shunt, hypertrophy of tumor vessel, or tumor staining extending to central portion of the mass in all cases. However, doughnut shaped peripheral tumor staining was seen until late hepatogram phase in 12 cases and compensatory hyperperfusion around the mass was seen in six cases(eight cases if include arterial CT). Encasement of tumor vessel was seen in 12 cases, and hypertrophy of feeding vessel in nine cases. On portogrphy, the filling defect on segmental portal branch could be demonstrated only in 11 cases. Shape of the portal defect was tapered narrowing in six cases, abrupt narrowing in two cases but intraluminal nodular filling defect was not seen. Remainning three cases were difficult to define the shape. On seven cases of CT during arterial portography, three cases showed mass shaped defect and four showed segmental defect but three of them could demonstrate the partially preserved portal flow in defective portal area. CONCLUSION: Hepatic arteriography in peripheral cholagiocarcinoma showed no evidence of hypertrophy of tumor vessels and tumor stain extending to central portion but peripheral staining on late hepatogram phase and compensatory hyperperfusion could be seen. Portal vein was more commonly involved through perivascular connective tissue invasion rather than by direct extension into the portal lumen.
Angiography*
;
Cholangiocarcinoma*
;
Connective Tissue
;
Hypertrophy
;
Portal Vein
;
Portography
;
Retrospective Studies
8.Patterns of Vascular Invasion of Intrahepatic Peripheral Cholangiocarcinoma Examined with Angiography and Angiographic CT.
Jae Chun CHANG ; Hyun Cheol CHO ; Won Kyu PARK
Journal of the Korean Radiological Society 1995;32(1):145-152
PURPOSE: To evaluate the radiological patterns of vascular invasion in peripheral cholangiocarcinomas. MATERIALS AND METHODS: Hepatic arteriography and portography in 20 cases with cholangiocarcinoma including 12 cases with anglographic CT were retrospectively analized. RESULTS: The arteriography showed no arterioportal shunt, hypertrophy of tumor vessel, or tumor staining extending to central portion of the mass in all cases. However, doughnut shaped peripheral tumor staining was seen until late hepatogram phase in 12 cases and compensatory hyperperfusion around the mass was seen in six cases(eight cases if include arterial CT). Encasement of tumor vessel was seen in 12 cases, and hypertrophy of feeding vessel in nine cases. On portogrphy, the filling defect on segmental portal branch could be demonstrated only in 11 cases. Shape of the portal defect was tapered narrowing in six cases, abrupt narrowing in two cases but intraluminal nodular filling defect was not seen. Remainning three cases were difficult to define the shape. On seven cases of CT during arterial portography, three cases showed mass shaped defect and four showed segmental defect but three of them could demonstrate the partially preserved portal flow in defective portal area. CONCLUSION: Hepatic arteriography in peripheral cholagiocarcinoma showed no evidence of hypertrophy of tumor vessels and tumor stain extending to central portion but peripheral staining on late hepatogram phase and compensatory hyperperfusion could be seen. Portal vein was more commonly involved through perivascular connective tissue invasion rather than by direct extension into the portal lumen.
Angiography*
;
Cholangiocarcinoma*
;
Connective Tissue
;
Hypertrophy
;
Portal Vein
;
Portography
;
Retrospective Studies
9.The influence of the fistula blood flow on the fistula patency after internal arteriovenous fistula.
Chang Nam KIM ; Won Hyun CHO ; Joong Shin KANG
Journal of the Korean Surgical Society 1992;42(4):525-532
No abstract available.
Arteriovenous Fistula*
;
Fistula*
10.A Case of Malignant Lymphoma Associated with Herpes Zoster, Neurogenic Bladder and Acquired Ichthyosis.
Kwang Hyun CHO ; Won Suk KIM ; Noe Kyeong KIM
Korean Journal of Dermatology 1979;17(4):259-263
Herpes zoster is a viral infection of the dorsal nerve root causing painful vesicular eruptions over the skin or mucous membrane of the affected nerve. It is less well known to the dermatologists that berpes zoster causcs bladder dysfunction when the zoster affects the lumbar and sacral spinal ganglia. The authors observed a case of herpes zoster involving the S 2~4 dermatomes, associated with bladder atony in a 58 year old male who had received rad.iotherapy and chemotherapy for his malignant Iymphoma of lymphocytic type. Conservative treatment with urinary catheterization and antibiotics resulted in complete recovery of the bladder function about 3 weeks later. He had also ichthyosiform skin lesions on his back and lower extremities which, by carefuI history and histopathological examination, proved to be acquired ichthyosis.
Anti-Bacterial Agents
;
Drug Therapy
;
Ganglia, Spinal
;
Herpes Zoster*
;
Humans
;
Ichthyosis*
;
Lower Extremity
;
Lymphoma*
;
Male
;
Middle Aged
;
Mucous Membrane
;
Skin
;
Urinary Bladder
;
Urinary Bladder, Neurogenic*
;
Urinary Catheterization
;
Urinary Catheters