Objective To investigate the effects of seven halothane and propofol anesthesia on heart rate and myocardial enzymes in elderly patients with coronary heart disease (CHD), and to analyze the safety of clinical application of the two kinds of anesthetic drugs.MethodsA total of 70 cases of elderly patients with coronary heart disease treatment January 2012 to December 2014 general anesthesia surgery as the research object, randomly divided into A group and B group were 35 patients in.A group were treated with seven sevoflurane anesthesia, group B patients were given propofol anesthesia, observe the change of the two groups of patients before and after anesthesia hemodynamic index and myocardial enzyme etc.index.ResultsA group of patients with HR 5min after induction and 10min were significantly lower than before the surgery, and the difference was statistically significant (P<0.05);HR stabilized after the induction of 15min and 20min, and compared with before surgery had no significant difference;patients in group B HR and 10min 5min after induction there was no obvious change compared with that before operation, no statistical significance;and after the induction of 15min and 20min HR increased, the difference was statistically significant (P<0.05).A group and B group in patients with systolic and diastolic blood pressure were lower than before anesthesia in varying degrees, and group B more significantly, the difference was statistically significant (P<0.05).A group and B group of patients after 6 h, cTnl and CK-MB in 12h and 24h than 15min before surgery has increased significantly, and A group cTnl and CK-MB were lower than B group, the difference was statistically significant (P<0.05).ConclusionThe clinical application of seven sevoflurane anesthesia for elderly patients with coronary heart disease during propofol is feasible, but seven halothane on blood pressure and heart rate of patients affected with propofol, improve the myocardial enzyme level, and better reduce the damage of perioperative myocardial, more suitable for anesthesia in elderly coronary heart disease.

Objective:To explore the effect of TSPG (total saponins of panax ginseng) on gene expression of IL-3 which is one of the important hematopoietic growth factor at early stage of hematopoiesis.Methods:Techniques of RT-PCR and in situ hybridization were used.Results:A band corresponding to IL-3 was observed after RNA extraction from TSPG stimulated Jurkat cells were subjected to RT-PCR,while such band was not observed after RNA extraction from unstimulated Jurkat cells were subjected to RT-PCR.Comparing with the control group,both quantity and intensity of IL-3mRNA expression in fetus thymocytes and splenocytes induced by TSPG were obviously promoted.Conclusion:TSPG can induce human lymphocytes to express IL-3 mRNA,thus promote human hematopoiesis at early stage.

Objective:To establish a stable and effective system of mouse embryonic fibroblasts(MEFs) feeder layer in order to separate and culture mouse embryonic stem cells(ESC) in vitro.Methods:the MEFs were isolated from the mouse embryos at 13.5~14.5dpc(days post-coitus) through primary tissue digestion and tissue piece culture.The optional planting density of cell was determined according to the cellular growth curve detected by MTT and the morphology in the culture dish under inverted microscope.The optional concentration of mitomycin C and the time treating with mitomycin on MEFs were determined by MTT assay.Murine blastcysts(3.5dpc) were cultured on the feeder layer treated by mitomycin C and ESC growth was observed.Result:The method of tissue digestion and tissue piece culture is an efficient way to obtain primary MEFs;1?105~2?105/ml cells is an optional planting density;MEFs proliferation could be effeciently repressed after being treated with mitomycin C 10?g/ml for 3.5 hours or 20?g/ml for 2.5 hours,and the quantity of MEFs could maintain at least for 7 days.ES clone could be obtained from the mouse blastcysts(3.5dpc) after being cultured on the feeder layer.Conclusion:MEFs feeder layer could effectively support mouse embryonic stem cells to grow.

Objective Panax ginseng is a well-known Chinese traditional medicine functional in “invigorating qi”,and saponins are one of its main effective fractions.Our study investigates the effect of total saponins of panax ginseng(TSPG)on human hematopoiesis at early stage and its possible regulative mechanism to clarify the hematonic mechanism of Panax ginseng. Methods The techniques of culturing hematopoietic progenitor cells in vitro,bioassay of hematopoietic growth factor(HGF),immunocytochemistry and nucleic acid in situ hybridization were used to study the effect of TSPG on IL-3’s expression in hematopoietic stromal cell and its possible mechanism. Results TSPG directly added into culture system in vitro can markedly increase the colony forming yields of CFU-Mix;the different conditioned culture media prepared with TSPG can promote the proliferation and differentiation of CFU-Mix;the protein and mRNA expression of IL-3 in BMSC and cells of EcV304,THP induced by TSPG has been much intensified.Conclusion TSPG may regulate hematopoiesis at early stage by activating IL-3's gene expression in hematopoietic stromal cell,which is the most important element in hematopietic inductive microenvironment(HIM).

PBL teaching method can cultivate students’ ability to analyze the existing problems, find out more problems and resolve the problems finally, and it is important to improve students’ comprehensive quality. The experimental teaching innovation of Histology and Embryology is summarized in this article, which turned induction, practice and examination, the three stages of experimental teaching into" Problem-Based learning", thus making the teaching effect much better.

Objective This article aims to provide mechanisms and recent developments of molecular biology pertaining to re-sistance of Enterococci,providing rapid approaches for detecting resistant strains.Methods This article reviewed recent lit-eratures on resistance of Enterococci and a systemic analysis was conducted.Results Common detecting methods include polymerase chain reaction (PCR),pulsed field gel electrophoresis (PFGE),multilocus sequence typing (MLST)and South-ern blot.There also exist less widely-used methods such as pyrosequencing and genechip technique,which may prove effi-cient in some aspects.Conclusion Every method has its advantages and disadvantages.This article discussed how to utilize these methods to achieve their maximum capabilities.

Objective To explore the clinical outcomes of vestibular rehabilitation therapy(VRT) in the treat‐ment of patients with balance disorders .Methods Seventy -six patients were diagnosed with vertigo based on the principle of single and double side targeted rehabilitation ,and were divided into four groups randomly .Group A of 29 cases received unilateral simple drug treatment only ,group B of 29 cases received unilateral drug treatmeat com‐bined with vestibular rehabilitation therapy ;bilateral simple drug treatment for the 9 cases in group C ,group D of 9 cases accepted bilateral drug treatment combined with vestibular rehabilitation therapy .Group A and C were the control groups while B and D were experimental groups .All cases were retrospectively analyzed to compare vestibu‐lar rehabilitation therapy combined with drugs treatment with the simple drug treatment group .Results The effects in all patients were better after treatment .The VSI scores four weeks after treatment in four groups were signifi‐cantly lower than that of before treatment .The BBS score after treatment of experimental groups were significantly higher than those of in the control groups .The Fukuda step experiment effects were better than the control group . All the above comparisons had a statistical significance(P< 0 .01) .Conclusion The clinical efficacy of normative vestibular rehabilitation exercise is satisfactory .

The pathogenesis of drug-induced Iiver injury( DILI)remains uncIear. AccumuIating evidences in recent years showed that immunoIogicaI factors pIay important roIe in the pathogenesis of DILI. In this articIe,we summarized recent studies on immunoIogicaI factors invoIved in pathogenesis of DILI and compared the immunity mediated DILI with drug induced autoimmune hepatitis. The advances in study on immunoIogicaI factors in DILI wiII have significant impact on prevention and treatment of DILI and autoimmune hepatitis.

Objective To discuss about the application of early restricted fluid resuscitation in dealing with thoracic and abdominal trauma combined with hemorrhagic shock.Methods 140 cases of patients with thoracic and abdominal trauma combined with hemorrhagic shock were divided into two groups stochastically,one group was dealt with conventional liquid resuscitation (the control group,71 cases),the other group was treated with limited fluid resuscitation (the limited group,69 cases).The patients of the control group were treated substantial rehydration therapy,the rehydration was carried out quickly and sufficiently in the early before hemostasis,to keep the mean arterial pressure (MAP) at 80～90 mmHg (1 mmHg=0.133 kPa); The amount and velocity of entering liquid of the limited group was under controlled,their MAP was kept at 55～65 mmHg,Adequate fluid resuscitation was carried out after bleeding was stopped.The average amount of infusion,the general situation,the incidences of complications and the death rates of two groups were compared.Results The average amount of infusion in the control group was significantly more than the limited group,the preoperative Hb and HCT of the control group were obviously lower than the limited group,the incidence of complications and the death rate of the control group were higher than the limited group.Conclusions If preoperative restricted fluid resuscitation for traumatic shock was used,the Harassing for Organism's compensatory mechanisms and internal environment could be avoided,the incidence of complications and the death rate will become lower,therefore,this method was worth to be extended in the clinical operation.

Objective The clinical characteristics and treatment outcomes of Listeria monocytogenes (LM) meningitis were reviewed. Methods The clinical data of a case of LM meningitis were retrospectively analyzed and a literature review was performed. Results The child initially presenting with fever, headache and vomit was treated with amoxicillin/clavulanic acid and ceftriaxone and the treatment effect was obvious. Cerebrospinal fluid and blood cultures were LM positive. In 17 related arti-cles, a total of 24 children with LM meningitis were reviewed. Among them, 4 patients died and 10 had hydrocephalus. The treatment with either ampicillin alone or in combination with aminoglycoside was effective. Conclusions LM meningitis was rare in immunocompetent children, but has high rates of mortality and sequelae.