The lack of donor corneal endothelium is a serious impediment to the development of corneal endothelial transplantation,whereas the bioengineered cornea provides an approach to this problem.Functional corneal endothelial cells which differentiated from human embryonic stem cells (ESCs) can,to some extent,relieve the lack of donor corneas,especially for corneal endothelia.At the moment,an optimal way of offering bioengineered-corneal endothelium is to cultivate the corneal endothelial cells in vitro.This is a process of inducing human ESCs to differentiate into neural crest stem cells (NCSCs) and then into corneal endothelial cells in a favorable medium with growth factors and extracellular matrix,which are matched microenvironment of endothelial cells in vitro.However,the inducement condition is still pending and remains for further research.This article reviewed the researching development of bioengineered-corneal endothelium from the effect of microenvironment and the induction of human ESCs.

0.05).TGF-?1 of patients with PHN,trigeminal and glossopharyngeal neuralgia either administered NSAIDs drugs or not were significantly lower than patients with Cervical spondylosis and lumbar disc herniation either administered NSAIDs drugs or not respectively(P

The vetting of senior professional positions in hospitals is related not only to the development of staff members,but also to the quality of the medical service.It is a multiple-stage process,which involves individuals and every department of a hospital,and is associated with a good understanding of policy and professional knowledge.Naturally,the vetting of senior professional position may bring about many new conditions and problems now and then,which pose challenges that need to be perceived and solved in time.The this article focuses on the resolution of the problems that may arise during the vetting process of senior professional positions of a hospital,and proposes suggestions for the improvement of the vetting.

This study aimed to analyze about the perception in obesity and body somatotype of university students in Korea and in China. This study provides the basic data of nutrition education for university student healthy weight program in China. The subjects were selected 240 university students of Korea and China. Two types of qualitative and quantitative questionnaires were used to analyze the attitude and body somatotype of subjects. The results of this study were as follows: The average BMI of Korean and Chinese male students was 22.3 and 22.5, respectively while the average BMI of Korean and Chinese female students was 19.8 and 19.7, respectively. In the past three years, the weight gain of Chinese students was higher than that of Korean students. Chinese students preferred the overweight body somatotype more compared to the Korean students. The overweight and obese students had more obvious insufficiency in body somatotype perception. The overweight and obese students had higher tendency to 'eat more meat', 'drink carbonated beverages', 'eat convenience food', 'take fast food' and 'drink alcohol' than the normal and low weight group. The major reasons for Korean students to control weight were 'appearance' and 'self confidence', while the major reasons for Chinese students were 'health' and 'employment'. From the results of associative group analysis (AGA), Chinese students had different semantic value of 'obese' than Korean students. Considering of the food transition status in China, it may be necessary to develop more suitable education programs for weight control for Chinese university students.
Asian Continental Ancestry Group ; Carbon ; China ; Female ; Humans ; Korea ; Male ; Obesity ; Overweight ; Surveys and Questionnaires ; Semantics ; Somatotypes ; Weight Gain

Objective To investigate the distribution and variety of quinolone-resistance genes in a group of multiple resistant Klebsiella.Methods A total of 20 strains of multiple resistant Klebsiella were collected from inpatients in Huai' an First People' s Hospital of Nanjing Medical University during February 2010 and March 2012.Strains were identified by molecular identification,and quinolone target genes (gyrA,ParC) and quinolone-resistance genes mediated by mobile genetic elements (qnrA,qnrB,qnrS,aac (6′)-Ⅰ b-Cr,qepA) were analyzed with polymerase chain reaction (PCR).Results In 20 strains of Klebsiella,19 were Klebsiella pneumonia,and 1 was Klebsiella variicola.gyrA and parC genes were found in all 20 strains,and gyrA mutation was found in 11 strains (55.0％),andparC mutation was also found in 11strains (55.0％).aac(6′)-Ⅰ b-cr was positive in 10 strains (50.0％),qnrA was positive in 1 strain (5.0％),and qnrB was positive in 3 strains (15.0％).gyrA and parC in strain No.6 and No.10 were both novel variants (GenBank registration number:JX123016,JX123017,JX144393,JX144394).Conclusion Quinolone-resistance-determining region plays a key role in resistance to quinolones in this group of Klebsiella,and it is the first report on the emergence of novel variants of gyrA and parC in one Klebsiella pneumonia in China.

This article briefly introduces how to rationally use antibiotics in patients with strokeassociated pneumonia from the aspects of etiology,disease,and antibiotic use.A great importance should be attached early to the standard pathologic examination,and the initial empiric treatment should be timely and adequate.The complex preparation of broad-spectrum penicillin/β-lactamase inhibitor is generally recommended,on this basis,the etiological examination results should be sought actively.Selecting the highly targeted or sensitive antibiotics improve the efficacy and reduce adverse reactions.

BACKGROUND: Poly methyl methyacrylate (PMMA) has been widely used as a denture base material in dental field for a long time. However, the fracture of acrylic resin dentures is an unresolved problem in prosthodontics. Therefore, how to improve the flexural strength of repaired denture seems to be extremely important. OBJECTIVE: To study the effect of embedding different amounts of metal wires on the flexural strength of repaired denture bases. METHODS: Twenty-five rectangular specimens (50 mm× 30 mm × 2.5 mm) were fabricated using heat-cured acrylic resins and randomly and evenly divided into five groups: A, B, C, D, and E. All specimens were fractured through the use of universal testing machine, and the flexural strengths were tested. Following preparation of fracture surfaces, one to four metal wires were separately embedded in the groups B, C, D, and E. No metal wires were embedded in the group A. All fractured specimens were repaired using self-curing resins. The flexural strengths were measured again using the same testing machine, and the percentages of strength recovery were calculated. RESULTS AND CONCLUSION: After repair, the flexural strengths were obviously reduced (P < 0.01). Compared to group A, the percentages of strength recovery were significantly increased in groups C, D, and E (P < 0.05). There was no significant difference in the percentage of strength recovery among groups C, D, and E (P > 0.05). These results indicate that embedding two to four metal wires can improve the flexural strength of denture bases.

Glutathione S-transferase kappa 1 (GSTK1) is a key regulator for adiponectin secretion and multimerization.In Caenorhabditis elegans,GSTK1 is involved in energy production and lipid metabolism.Meanwhile,the GSTK1 level is negatively correlated with obesity.It may alleviate the endoplasmic reticulum stress-mediated downregulation of adiponectin.Moreover,a polymorphism in human GSTK1 promoter is related with insulin secretion and fat deposition.Therefore,GSTK1 might be a novel target for the treatment of insulin resistance and the relevant metabolic diseases.

The ischemic heart disease has been endangering human health seriously. Although there are many kinds of anti-ischemic drugs, most of them are lacking in tissue specificity, which together with a remarkably reduced blood circulation in the ischemic zone often lead to a quite low drug distribution in the targets. Myocardial ischemia can cause a lot of pathophysiological changes, such as the enhanced permeability of the endothelial cell membrane, the up-regulated expression of various cell adhesion molecules on endothelium, the exposure of intracellular antigenic components, the decrease of pH within the ischemic zone, and so on. To date, some of these changes have been exploited with limited success to gain the passive, active and physicochemical targeting of diagnostic or therapeutic drugs to myocardial ischemic regions. However, more effective delivery strategies are still eagerly needed. Here, we reviewed and discussed the potential targeting-delivery mechanisms and strategies, used or may be used in the future, for myocardial ischemic regions.

Objective To assess the efficacy and adverse reactions of two cconcentrations of diluted botulinum toxin A for dynamic frontotransverse wrinkles.Methods Totally 103 cases met the inclusion criteria were assigned into two groups by a random number table:high concentration group (50 U/ml,53 cases) and low concentration group (25 U/ml,50 cases).The two groups were given the local injection with 50 U/ml and 25 U/ml concentration of botulinum toxin type A,respectively.Dosage of BTX-A,the treatment effect,the duration of the effect and the incidence of adverse reactions were analyzed.Results The high concentration group were given the BTX-A (45.53±2.05) U,the low concentration group were given the BTX-A (25.71 ± 1.74) U,and the difference were statistically significant (P＜0.05).The differences of post-operative evaluation,time of onset and duration between the two groups were not statistically significant (P＞0.05).In the high concentration group,30 cases (88.24％) had forehead heaviness sensation,and 2 cases (5.71％) in the low concentration group,the difference were statistically significant (P＜0.01).Neither of the groups was given any specific treatment,the symptoms were disappeared in two weeks.In either group there was no serious complications such as intoxication,allergy,etc.Conclusions The efficacy of local injection with two concentrations of diluted botulinum toxin A in the treatment of dynamic frontotransverse wrinkles is positive,the duration of treatment effect between the two groups is similar,and therefore the 25 U/ml concentration is recommended to reduce the forehead heaviness sensation.