1.Morphologic Characterization of Polycystic Kidney in inv Transgenic Mouse.
Yeon Lim SUH ; Mi Kyung KIM ; Joungho HAN
Korean Journal of Pathology 1998;32(7):479-487
The aim of this study was to characterize the morphology of a polycystic kidney which was found in 100% of the transgenic mice homozygous for inv mutation and to gain insight into the pathogenesis of inherited polycystic kidney disease during the pre- and postnatal periods. The fetal and postnatal kidneys from the homozygous and heterozygous transgenic mice were examined by the light, transmission and scanning electron microscopes, image analyzer, and an immunohistochemistry utilizing the antibodies specific for each segment of the renal tubules (Tetragonolobus purpureas, Arachis hypogaea, Tamm-Horsfall protein, AE1/AE3, EMA, vimentin, Phaseolus vulgaris) was performed to determine the site of origin of renal cysts. Two developmental phases of a cystic disease were identified. The first phase, seen in fetal kidneys, was characterized by dilatation mainly of the proximal tubules and a few distal tubules. The later phase, in postnatal period, was characterized by progressive enlargement of the kidneys due to mainly cystic change of the collecting ducts, which distorted the normal architecture of both cortex and medulla and almost completely replaced the renal parenchyma. The cystic dilatation involved all segments of the nephron and the collecting duct as well as the Bowman's spaces of glomeruli. The epithelial cell hyperplasia was found as a micropolyp formation within the renal cysts and an increase in PCNA positive cells. These findings suggest that a cyst is not simply a ballooning of a renal tubule and the stretching of cells, formerly thought to be due to an altered compliance of an abnormal basement membrane, but indeed the result of increased numbers of tubular epithelial cells.
Animals
;
Antibodies
;
Arachis
;
Basement Membrane
;
Compliance
;
Dilatation
;
Epithelial Cells
;
Hyperplasia
;
Immunohistochemistry
;
Kidney
;
Mice
;
Mice, Transgenic*
;
Microscopy, Electron
;
Nephrons
;
Phaseolus
;
Polycystic Kidney Diseases*
;
Proliferating Cell Nuclear Antigen
;
Uromodulin
;
Vimentin
2.Ultrastructural Study of Vitiligo.
Chan Woo JEONG ; Il Yeong SON ; Un Cheol YEO ; Joungho HAN ; Eil Soo LEE
Annals of Dermatology 2001;13(3):158-162
BACKGROUND: There is a long-standing controversy whether melanocytes in vitiligo of more than 1 year duration are actually lost or still present. Resolving this matter is essential in understanding the underlying pathology and for the development of the treatment. On previous immunohistochemical and ultrastructural studies of vitiligo lesions, damage of melanocyte and keratinocyte in early lesions were reported and complete absence of melanocyte in long standing lesions were known. OBJECTIVE: This study aimed to determine the existence of the differences in pathologic changes in melanocytes according to the duration of the lesion. METHODS: We investigated the vitiliginous skin samples from 31 patients with early(less than 1 year duration) vitiligo and 30 patients with long standing(l to 5 years duration) vitiligo under the electron microscopy. RESULTS: Multiple degenerative changes in melanocytes were observed in the early and long standing lesions. In long standing lesions, degeneration of melanocytes including pyknotic, in-dented nuclei, vacuolated cytoplasms and blunted dendrites were more pronounced than early lesions. Even in long standing lesions, definite or presumptive melanocytes were observed in 16(53.3%) of 30 cases. CONCLUSION: Our results suggest that the melanocytes of vitiligo lesions were damaged and that the percentage of degenerative changes increase in accordance with the duration of the lesion. However, in long standing lesions as well as in early lesions, some residual melanocytes can be observed ultrastructurally.
Cytoplasm
;
Dendrites
;
Humans
;
Keratinocytes
;
Melanocytes
;
Microscopy, Electron
;
Pathology
;
Skin
;
Vitiligo*
3.Nasal Rhinoscleroma in a Nonendemic Area: A Case Report.
Na Rae KIM ; Joungho HAN ; Tae Young KWON
Journal of Korean Medical Science 2003;18(3):455-458
Rhinoscleroma is a chronic, granulomatous infectious lesion most frequently affecting the respiratory tract mucosa. This disease is endemic to Africa, Central and South America, South Central and Eastern Europe, the Middle East, and China. We report an extremely rare disease of nasal rhinoscleroma in Korea. The patient was a 63-yr-old man, who suffered from chronic nasal obstruction and septal deviation. Characteristic histology from a nasal biopsy was proven and special stains for bacteria were employed: periodic acid-Schiff, Warthin-Starry silver, Giemsa, and Gram stains. Isolation of Klebsiella rhinoscleromatis from a culture of nasal discharge was failed, but ultrastructural examination of numerous phagocyzed bacilli in the Mikulicz cells confirmed the diagnosis. The histiocytic nature of the Mikulicz cells was confirmed, using CD 68 and alpha-1 antitrypsin, and by the ultrastructural features of Mikulicz cells. Here, we emphasize the recognition of this rare entity in nonendemic regions, frequently leading to delayed diagnosis.
Endemic Diseases
;
Fibrosis
;
Human
;
Klebsiella pneumoniae/*isolation & purification
;
Korea
;
Male
;
Microscopy, Electron
;
Middle Aged
;
Nasal Mucosa/*microbiology/pathology/ultrastructure
;
Rhinoscleroma/*pathology
4.A Case of Extramammary Paget's Disease on the Scalp.
Il Yeong SON ; Hwan Tae SUNG ; Ho Su CHUN ; Joungho HAN ; Eil Soo LEE
Annals of Dermatology 1999;11(3):189-192
We treated a case of extramammary Paget's disease that affected the scalp of a 45-year-old female. It is extremely rare that the disease arises in areas other than the anogenital region and the axillae. The lesion was a round erythematous oozing crusted hairless patch. Histopathologically, many pagetoid cells were found within the epidermis and dermis. The cytoplasms of these cells stained with alcian blue at pH 2.5, CEA, EMA and low-molecular-weight-cytokeratin. The patient underwent a wide local excision. We used a mapping technique to reveal the distribution of microscopically involved lesions.
Alcian Blue
;
Axilla
;
Cytoplasm
;
Dermis
;
Epidermis
;
Female
;
Humans
;
Hydrogen-Ion Concentration
;
Middle Aged
;
Paget Disease, Extramammary*
;
Scalp*
5.Localized Primary Thymic Amyloidosis Presenting as a Mediastinal Mass: A Case Report.
Sang Yun HA ; Jae Jun LEE ; Heejung PARK ; Joungho HAN ; Hong Kwan KIM ; Kyung Soo LEE
Korean Journal of Pathology 2011;45(Suppl 1):S41-S44
We herein describe a case of a 55-year-old healthy woman with localized primary thymic amyloidosis presented as a mediastinal mass, found incidentally by chest radiography. Computed tomography revealed a 4.1 cm soft tissue lesion with nodular calcification in the left anterior mediastinum. The resected specimen was a well-defined lobulating mass with calcification. Microscopically, the mass was consisted of amorphous eosinophilc hyalinized substances involving the thymus and intrathymic lymph nodes. These eosinophilic substances showed apple-green bi-refringence under polarized light after staining with Congo red. In immunohistochemical study, they were positive for kappa and lambda light chains and negative for amyloid A. There was no evidence of systemic amyloidosis in clinical investigations. A final diagnosis of localized primary thymic amyloidosis was made.
Amyloid
;
Amyloidosis
;
Congo Red
;
Eosinophils
;
Female
;
Humans
;
Hyalin
;
Light
;
Lymph Nodes
;
Mediastinum
;
Middle Aged
;
Thorax
;
Thymoma
;
Thymus Gland
6.Pathologic Review of Cystic and Cavitary Lung Diseases.
Korean Journal of Pathology 2012;46(5):407-414
Pulmonary cystic and cavitary lesions caused by diverse etiologies are commonly encountered in chest imaging. The terms "cyst" and "cavity" are used to describe air-filled regions in the center of a nodule or consolidation of the lung. To date, only radiologic aspects of these lesions have been addressed. The morphologies of pulmonary cystic and cavitary lesions exhibit a broad spectrum, ranging from benign to malignant pulmonary diseases of acquired or congenital origin, including variable infectious diseases. In this review, we summarized the differential diagnosis of pathological entities to provide pathologists and radiologists with an overview of the diseases most commonly associated with pulmonary cystic and cavitary lesions in adults and children. The results showed slightly different patterns in the distribution of the diseases in the two groups. The most common causes of cavitary lesions include malignancy and infection in adults, and congenital malformation in children. Therefore, identification of pathologic entities correlating with the radiologic findings, clinical course, and location of the lesion is important in the evaluation of cystic and cavitary lung lesions in order to avoid unnecessary surgical procedures or delayed treatment.
Adult
;
Child
;
Communicable Diseases
;
Diagnosis, Differential
;
Humans
;
Lung
;
Lung Diseases
;
Thorax
7.Supradiaphragmatic Heterotopic Liver Presenting as a Pleural Mass: A Case Report.
Jungsuk AN ; Joungho HAN ; Kyung Soo LEE ; Yong Soo CHOI
Tuberculosis and Respiratory Diseases 2010;69(3):191-195
Abnormally located liver tissue has been described in the vicinity of the liver proper, near anatomical structures such as the gallbladder, the umbilical fossa, the adrenal gland, the pancreas, and the spleen. Supradiaphragmatic ectopic liver is a rare finding, but has been reported to have been found in the intrathoracic cavity and in the pericardium. In the majority of supradiaphragmatic ectopic liver cases, there was an accompanying transdiaphragmatic pedicle of the main liver body into the abdominal cavity. In a minority of supradiaphramatic ectopic liver cases, the liver was completely separated from the abdominal cavity without a connection between the thorax and the abdomen, with accompanying diaphragmatic anomalies. We describe one case of intrathoracic ectopic liver in a patient with a previous history of lower chest wall trauma, and a brief review of the English-language medical literature on this topic.
Abdomen
;
Abdominal Cavity
;
Adrenal Glands
;
Diaphragm
;
Gallbladder
;
Humans
;
Liver
;
Pancreas
;
Pericardium
;
Spleen
;
Thoracic Wall
;
Thorax
8.Supradiaphragmatic Heterotopic Liver Presenting as a Pleural Mass: A Case Report.
Jungsuk AN ; Joungho HAN ; Kyung Soo LEE ; Yong Soo CHOI
Tuberculosis and Respiratory Diseases 2010;69(3):191-195
Abnormally located liver tissue has been described in the vicinity of the liver proper, near anatomical structures such as the gallbladder, the umbilical fossa, the adrenal gland, the pancreas, and the spleen. Supradiaphragmatic ectopic liver is a rare finding, but has been reported to have been found in the intrathoracic cavity and in the pericardium. In the majority of supradiaphragmatic ectopic liver cases, there was an accompanying transdiaphragmatic pedicle of the main liver body into the abdominal cavity. In a minority of supradiaphramatic ectopic liver cases, the liver was completely separated from the abdominal cavity without a connection between the thorax and the abdomen, with accompanying diaphragmatic anomalies. We describe one case of intrathoracic ectopic liver in a patient with a previous history of lower chest wall trauma, and a brief review of the English-language medical literature on this topic.
Abdomen
;
Abdominal Cavity
;
Adrenal Glands
;
Diaphragm
;
Gallbladder
;
Humans
;
Liver
;
Pancreas
;
Pericardium
;
Spleen
;
Thoracic Wall
;
Thorax
9.Micronodular Thymoma with Lymphoid Stroma: A Case Report.
Hye Jong SONG ; Joungho HAN ; Tae Sung KIM
Journal of Lung Cancer 2011;10(1):56-58
Micronodular thymoma with lymphoid stroma (MNT) is an extremely rare tumor and has not been reported in Korea. Herein, we report a case of MNT diagnosed in a 75 year-old male. The mass was incidentally identified on thoracic computer tomography (CT) during work-up for a cardiac mass. The resected thymic mass was ovoid and solid measuring 3.5x3 cm in size and showed homogenously white tan, solid and firm cut surface. Microscopically, the tumor had a thin fibrous capsule except small foci of invasion to thymic adipose tissue, and consisted of characteristic multiple discrete epithelial nodules in abundant lymphoid stroma. The epithelial nests anastomosed each other, forming a vague cord-like structure. The cells in the epithelial nests were bland, spindle to oval shape with relatively abundant cytoplasm, slightly vesicular chromatin and one definite nucleolus. The lymphoid stroma contained prominent germinal centers. No evidence of recurrence or metastasis has been found within 4 years of surgery.
Adipose Tissue
;
Chromatin
;
Cytoplasm
;
Germinal Center
;
Humans
;
Korea
;
Male
;
Neoplasm Metastasis
;
Recurrence
;
Thymoma
;
Triacetoneamine-N-Oxyl
10.Bizarre Leiomyoma of the Scrotum.
Na Rae KIM ; Chang Ohk SUNG ; Joungho HAN
Journal of Korean Medical Science 2003;18(3):452-454
Scrotal leiomyomas with atypical bizarre nuclei are rare, which might be misdiagnosed as malignant tumor. We describe a case of scrotal bizarre leiomyoma in a 65-yr-old man. The tumor was a 1 cm-sized, well circumscribed, oval mass arising from the tunica dartos muscle. Histologically, it was formed by whorling bundles of fusiform cells with occasional atypical, pleomorphic nuclei and pseudoinclusions. Mitosis was not found. Although morphologically atypical, scrotal bizarre leiomyomas take on a biologic behavior not different from that of conventional leiomyoma, they should be distinguished from leiomyosarcoma to avoid unnecessary treatment.
Aged
;
Genital Neoplasms, Male/*pathology/surgery
;
Human
;
Leiomyoma/*pathology/surgery
;
Male
;
Scrotum/*pathology