The purpose of foreign language teaching in Chinese medicine is not only translation,but also clearly the role and the purposes of translation.Inputing a culture to another culture is not culture exchange,but cultural coagulation.Expressions with modem scientific system allow speculative natural philosophy freed from the ancient Chinese-style paper into the theory expressed by modem scientific language in order to solve the problems of teaching foreign languages of traditional Chinese medicine texts.

Objective To improve the current method of spermatogonial cells transplantation, and make the new method become more operative and easy to carry out. Methods The spermatogonial cells in rC57BL6/tg14 (act-EGFO-Osb Y01) mice that expresse GFP protein were collected as the donor cells, and by using a modified syringe, and then were injected into the seminiferous tubules of pretreated wild type GFP-null mice through testicular efferent duct. The transplantation outcome was evaluated by trypan blue stainting and fluorescent microscopic examination and pathohistological analysis of transplanted testis. Results The transplanted testis of recipient mice showed green fluorescence signal, and the signal of seminiferous tubules was found significantly higher than that of the surrounding tissues. The transplanted GFP-positive cells generated colonies and spermatogenesis but pretreated wild type GFP-null mice were not found. Conclusion The expressed GFP protein spermatogonial cells in rC57BL6/tg14 mice were successfully transplanted in the wild type GFP-null recipient mice, fourthermore the improved transplantation method simplified the triditional one and achieved the same transplantation results.

Cluster Analysis ; Fuzzy Logic ; Humans ; Mathematics ; Medicine, Chinese Traditional

OBJECTIVE In order to explore and control the risk factors of nosocomial infection in intracerebral hemorrhage.METHODS To study prospectively in 837 hospitalized patients with intracerebral hemorrhage in our hospital from Jan.1997 to June 1999. RESULTS It was showed 215 cases of the nosocomial infection were found among 837 cases with the intracerebral hemorrhage(25.8%).Among them,nosocomial lower respiratory tract infection was the highest(39.1%),the second one was the urinary tract(27.8%).Nosocomial infection was closely related with such factors as the aging,location and volume of intracerebral hematoma,disturbance of consciousness, smoking,blood sugar,invasive operation,antibiotic prophylaxis,bed-days.Whereas serum cholesterol was a protective factor.CONCLUSIONS Nosocomial infection was still a high frequent complication in intracerebral hemorrhage and it should be paid attention.This is the most important step to control nosocomial infection of intracerebral hemorrhage in the hospital.

Based on the report of previous clinical study which showed cholinergic receptor antagonist scopolamine had antidepressant activity, this study was to investigate the antidepressant activity of scopolamine and explore its effective dose in mice, and to evaluate the effect of scopolamine on the central nervous system and learning/memory ability at its antidepressant effective dose. Tail suspension test, forced swimming test, step-down passive avoidance test and open field test were used to evaluate its effects on mice. Compared with the vehicle control group, single-dose administration of scopolamine (0.1-0.4 mg x kg(-1), ip) significantly decreased the immobility time (P < 0.01 or P < 0.001) in tail suspension test, and significantly decreased the immobility time (P < 0.001) in forced swimming test, but had no effect on the step-down latency and errors in step-down passive avoidance test. Scopolamine (0.1 and 0.2 mg x kg(-1), ip) had no influence on the locomotor activity in open field test, while at dose of 0.4 mg x kg(-1) significantly increase the locomotor activity. These results showed that scopolamine produced reliable antidepressant effect at doses of 0.1 and 0.2 mg x kg(-1), without impairment on learning and memory, as well as excitory or inhibitory effect on central nervous system in mice.

BACKGROUND: Fetal microchimerism is a kind of stem cells which comes from fetus in pluripara. The research on fetal microchimerism is a hotspot because it may affect the health condition of pluripara.OBJECTIVE: To introduce the methods of how to detect fetal microchimerism, and the function of fetal microchimerism during disease and tissue repair of the mother.METHODS: Fetal microchimerism was used as a key word, and papers published before 2011-05-20 were retrieved in PubMed. Repetitive papers were eliminated.RESULTS AND CONCLUSION: Totally 655 papers were retrieved, and finally 26 were reviewed. There are several methods used for fetal microchimerism detecting, and fetal microchimerism may pay an important role in the pathomechanism of pluripara autoimmune diseases, cancer and tissue repair.

Objective To investigate the value of BISAP scoring system in predicting the severity of acute pancreatitis.Methods Medline,EMBASE,Science Direct,Springer link,CBM,Cnki,Wan fang and VIP database were retrieved by computer between January 2000 and March 2013,and articles of BISAP score system in predicting the acute pancreatitis were collected according to strict inclusion criteria.Quality assessment was made by QUADAS scale.Meta-Disc1.4 software was used to analyze the heterogeneity of included articles and perform quantitative synthesis,as well as calculate the pooled sensitivity and specificity,positive likelihood ratio,negative likelihood ratio,and draw the ROC curve,and the results were presented with 95％ CI.Results A total of 11 articles were included,and there were 7 Chinese articles and 4 English articles.According to QUADAS scale,there were 4 articles of A grade,5 articles of B grade,2 articles of C grade.There were 6 studies using 2 as BISAP cut-off value,and 9 articles using 3 as cut-off value,while 4 studies using 2 cut-off values.Pooled analysis showed diagnostic odds ratio of 8.03 (95 ％ CI 5.66-11.38) when cut-off value was 2,and diagnostic odds ratio of 7.49 (95％ CI 5.35-10.49) when cut-off value was 3.There were moderate heterogeneity in both groups (I2 =63.3 ％,P =0.018; I2 =56.1％,P =0.019).When BISAP cut-off value was 2,the pooled analysis of sensitivity,specificity,positive likelihood ratio,negative likelihood ratio and area under curve (AUC) were 59％ (95％ CI56％-63％),82％ (95％ CI 80％-83％),3.50 (95％ CI2.96-4.14),0.45 (95％ CI0.36-0.56) and0.82; and when BISAP cut-off value was 3,the corresponding values were 44％ (95％ CI41％-47％),90％ (95％ CI 89％-91％),4.59 (95％ CI 3.31-6.37),0.64 (95％ CI 0.61-0.68) and 0.64.The former had a high sensitivity,low specificity and large AUC,while the latter had a low sensitivity,high specificity and small AUC.Conclusions The best cut-off value of BISAP in predicting SAP is 2.When cut-off value is 2,the misdiagnosis rate is low,and the false positive rate is in the acceptable range,which is suitable for clinical application.

Research of oncogenes related to thyroid cancer has an important significance to reveal pathogenesis and identify benign and malignant lesions.At the mean time,these genes are helpful to find the reliable and feasible detection means of early diagnosis for thyroid cancer.

Carotid artery atherosclerosis is a treatable and preventable cause of ischemic stroke.Increasing studies suggest that carotid plaque characteristics may be a superior means for predicting ipsilateral cerebrovascular events as compared with the degree of carotid artery stenosis.The major MRI features of vulnerable carotid plaques include large lipid-rich necrotic cores,intraplaque hemorrhage,plaque rupture,and calcification.This article reviews the major MRI features of vulnerable carotid plaques and their roles in predicting ipsilateral cerebrovascular events.

Objective To screen a thermostable urate oxidase-producing strain, optimize the fermentation conditions and study the enzymatic properties.Methods A urate oxidase-producing strain was screened from high temperature starter based on transparent circle method.Its 16S rDNA sequence was then amplified and analyzed.Meanwhile, the phylogenetic trees were built.Optimization of the fermentation conditions from this strain was carried out.The enzymatic properties of urate oxidase were studied.Results A urate oxidase-producing strain, named Bacillus subtilis ZX-6 by molecular identification, was obtained.The production of urate oxidase under the optimized conditons (135.9 U/L) was 133.7%higher than before.The optimum reaction temperature and pH were 45℃ and 7.6 respectively.The residual activity of urate oxidase at 37℃ for 48 h was still 17.2%.Conclusion The successful screening of a thermostable urate oxidase-producing strain and optimization of the fermentation conditions will lay a foundation for the further research.