1.99mTc-MDP uptake in hydroxyapatite ocular implants.
Korean Journal of Nuclear Medicine 1992;26(2):396-400
No abstract available.
Durapatite*
;
Technetium Tc 99m Medronate*
2.Comparison of functional images obtained by radionuclide angiocardiography and gated blood pool scan.
Korean Journal of Nuclear Medicine 1991;25(2):186-191
No abstract available.
Angiocardiography*
3.Genotoxicity of therapeutic dose of I analyzed by micronuclei test in the mouse bone marrow.
Korean Journal of Nuclear Medicine 1993;27(1):112-117
No abstract available.
Animals
;
Bone Marrow*
;
Mice*
4.Effect of chitosan on the elimination of intraperitoneally administered radiostrontium(Sr-85).
Ji Yeul KIM ; Kwang Yoon KIM ; Hee Seung BOM
Korean Journal of Nuclear Medicine 1993;27(2):293-297
No abstract available.
Chitosan*
5.A Comparison Study of Radiostrontium Chelation with Chitin, Chitosan, EDTA and DTPA.
Hee Seung BOM ; Young Ho KIM ; Ji Yeul KIM
Korean Journal of Nuclear Medicine 1998;32(4):391-395
PURPOSE: Chitin and chitosan are nontoxic natural chelators that chelate radiostrontium effectively. The purpose of this study was to compare radiostrontium chelation of chitin and chitosan with that of well known chemical chelators, namely EDTA and DTPA. MATERIALS AND METHODS: The chelaton rates of chitin, chitosan, EDTA and DTPA were compared using a column chromatography method (Sephadex G-25M, Sweden). Three kinds of chitins and four kinds of chitosans were used. All of them were water soloble. RESULTS: Phosphated chitosan showed the highest chelation yield of more than 97% at pH 7. All of chitins, chitosans, EDTA and DTPA showed chelation yield of more than 90% independent of varing pH level. CONCLUSION: Chitin and chitosan have similar chelation rate as compared with EDTA and DTPA.
Chelating Agents
;
Chitin*
;
Chitosan*
;
Chromatography
;
Edetic Acid*
;
Hydrogen-Ion Concentration
;
Pentetic Acid*
6.Studies on the Radiation Induced Apoptosis by Morphological and Biochemical Analysis in A431 Cells.
Ji Yeul KIM ; Hee Seung BOM ; Keun Hee CHOI
Korean Journal of Nuclear Medicine 1999;33(3):306-315
PURPOSE: We performed this study to evaluate the process of radiation induced apoptosis in A431 skin epithelial cancer cell line. MATERIALS AND METHODS: Low to high dose radiation (0, 2, 5, 10, 25 Gy) was given to A431 cells by Cs-137 cell irradiator. Apoptosis was evaluated by cell morphology, dye exclusion test, and DNA laddering. RESULTS: Cell viability decreased as the radiation dose increased. Number of apoptotic bodies increased as radiation dose increased. It increased most significantly at 12 hours after irradiation. Lactate dehydrogenase activity in culture medium increased according to radiation dose and time after irradiation. CONCLUSION:: Radiation-induced apoptosis which was the main course of cell death in A431 cells could be analyzed quantitatively by counting apoptotic bodies under microscope. Apoptosis increased as radiation dose increased.
Apoptosis*
;
Cell Death
;
Cell Line
;
Cell Survival
;
DNA
;
L-Lactate Dehydrogenase
;
Skin
9.Development of a Noble Dosimetry Using Metaphase Analysis and Micronuclei Assay of Bone Marrow Cells in Mice.
Jung Jun MIN ; Hee Seung BOM ; Young Ho KIM ; Hyun Joong YOON ; Ji Yeul KIM
Korean Journal of Nuclear Medicine 2000;34(1):74-81
PURPOSE: The purpose of this study was to develop in vivo dosimetries using both chromosomal aberrations and micronuclei in mice to assess biological effects of radiations. MATERIALS AND METHODS: Five each mice were irradiated with 0, 1, 2, 3, 4, 5, 10 Gy of Cs-137 gamma-rays. We scored numbers of chromosomal aberrations in metaphase spreads and numbers of micronuclei in bone marrow smears under light microscope, and obtained the dose-response relationships. We also examined the relationship between the two dose-response curves. RESULTS: The frequency of both chromosomal aberrations and micronuclei increased with dose, in a linear-quadratic manner. The delta, beta, and alpha coefficients were 0.0176, 0.0324, and 0.0567 for metaphase analysis (r=1.0, p<0.001) and 0.0019, 0.0073, and 0.0506 for micronuclei assay (r=1.0, p<0.001). The frequency of chromosomal aberrations and micronuclei in diffirent radiation doses was significantly correlated (r=0.99, p<0.01). CONCLUSION: In vivo dosimetry using either metaphase analysis or micronucleus assay was feasible in mice. These methods could be useful to evaluate biological effects of radiation.
Animals
;
Bone Marrow Cells*
;
Bone Marrow*
;
Chromosome Aberrations
;
Metaphase*
;
Mice*
;
Micronucleus Tests
10.Hot spleen in hemochromatosis.
Yun Keun LIM ; Sung Ryul KIM ; Hee Seung BOM ; Ji Yeul KIM
Korean Journal of Nuclear Medicine 1991;25(2):298-299
No abstract available.
Hemochromatosis*
;
Spleen*