6.Determination of Magnob1 and Honokiol in Baolong Pill By HPLC
Hui LI ; Baotian CHEN ; Li LIU
Journal of Zhejiang Chinese Medical University 2006;0(06):-
[Objective]To establish an HPLC method for the determination of magnolol and honokiol in Baolong Pill.[Methods]The samples were analyzed on an Agilent C18 column(4.6mm?250mm,5?m).The mobile phase was a mixture of MeOH-H2O (78∶22);the rate of flow was 1.0 m1/min;the detection wave-length was 294 nm.[Results]Calibration graph was linear in the range of 0.02~0.26?g,r= 0.999 9 for magnolol;0.012~0.156?g,r= 1 for honokiol.The recovery was 100.46%,RSD=1.00% for magnolol;100.76%,RSD=1.37% for honokiol.[Conclusion] The method is sensitive and its results are accurate with good reproducibility.It can be used for the quality control of Baolong Wan.
7.Artifactual Responses when Recording Auditory Steady-State Responses to Bone-Conduction Stimuli
Hui LIU ; Lingyan MO ; Jing CHEN
Journal of Audiology and Speech Pathology 1998;0(03):-
Objective To explore artifactual responses of auditory steady-state reponses (ASSRs) to bone-conduction stimuli.Methods The present study involved a group of subjects,aged between 5 to 39 months,with bilateral profound sensorineural hearing losse.Recorded were the reponses to multiple bone-conducted 0.5,1,2,4 kHz tone pips repeated at the rates of 77~101 Hz.All the subjects were sedated by Hydrate Chloride during the testing.Results Artifactual ASSRs were seen at 115 and 100.5 dB (re:1?N) (The above levels are equivalent to around 60 dB HL) at 1 and 4 kHz.No artifactual responses were recorded at 0.5 and 2 kHz stimuli at the maximum output level.Conclusion Bone-conducted hearing loss greater than moderate can not be tested reliablely due to artifactual responses and lower maximum output level than that of air conduction.
8.Effect of ?-amyloid_(1-40) on the expression of nitric oxide synthase in rat brain
Hui LIU ; Junpao CHEN ; Shiyu TIAN
Chinese Journal of Neurology 2001;0(02):-
Objective To investigate the role of nitric oxide synthase (NOS) in neurotoxic mechanisms of beta amyloid (A?) and the pathogenesis of Alzheimer′s disease (AD). Methods 35 adult rats were divided into 3 groups: normal control,saline injection,and A? 1 40 injection groups A? 1 40 was initially solubilized in saline (10?g/?l) and incubated for at least 1 week before use 1?l incubated A? 1 40 or saline were injected into dorsal blade of dentate gyrus in right hippocampus of rats Immunohistochemical assay of neuronal NOS (nNOS) and inducible NOS (iNOS) was performed at the 2 nd,10 th,and 30 th day after A? 1 40 or saline injection. Results The nNOS like immunoreactivity (nNOSLIR) neurons were found in different brain regions such as cortex,striatum and hippocampus of normal rats,and the number of neurons in dentate gyrus was 8 96?0 31 The number and configuration of nNOSLIR neurons were found without changes after saline injection (8 97?0 29) At 2,10,and 30 d after A? 1 40 injection,the number of nNOS neurons at the site around injection was significantly reduced (3 13?0 27,2 89?0 19 and 2 91?0 25 respectively),but there were no notable differences at these three experimental time points iNOS like immunoreactivity (iNOSLIR) cells were not found in any brain region of normal or saline injected rats After A? 1 40 injection,a large percentage of glia (mainly were astrocytes) had an activated morphological change and showed a strong iNOSLIR around the injection site The responded areas with these cells were 0 905?0 082,0 962?0 161 and 0 935?0 125 mm 2 respectively at 2,10 and 30 day after A? 1 40 injection,but there appeared no significant differences among them. Conclusions The results demonstrate that intrahippocampal injection of A? 1 40 may result in a significant loss of nNOS neurons and induce a considerable expression of iNOS in astrocytes,indicating that NOS may play an important role in neurotoxic mechanisms of A? and the pathogenesis of AD
9.Hepatoprotective Effect of Sabia parviflorawall:an Experimental Study
Yirong LIU ; Xiaochun QIU ; Hui CHEN
China Pharmacy 2007;0(30):-
OBJECTIVE:To study the hepatoprotective effect of the extract of Sabia parviflorawall(SPS) on mice with experimental liver injury.METHODS:The liver injury models were reproduced in mice with CCL4 and Paracetamol(AAP),respectively with the activities of serum ALT and AST measured.RESULTS:The activities of serum ALT and AST were all reduced to a certain degree in mice with liver injury induced by either CCL4 or Paracetamol(AAP).CONCLUSION: SPS exhibited certain hepatoprotective effect.
10.Molecular Typing of Trichophyton rubrum Isolated from Multiple Skin Lesions
Hui CHEN ; Weida LIU ; Yongnian SHEN
Chinese Journal of Dermatology 2003;0(12):-
Objective To determine whether Trichophyton rubrum isolated from different lesions in the same patient is of different strains. Methods DNA was extracted from the isolates, then subjected to a polymerase chain reaction-based typing which analyzed the number of repetitive elements in the non-transcribed spacer region of the ribosomal DNA gene repeats. Results Thirty-six strains of T. rubrum were isolated from 17 patients with fungal infection on multiple sites. All strains could be classified into 10 genotypes. The genotype distribution was unrelated to sites of infection. It happened to 8 of the 17 patients that multiple genotypes were involved in T. rubrum infection on different sites in the same body. Conclusion The study shows that multiple genotypes are involved in T. rubrum infection on different sites in the same patient, suggesting external sources of infection rather than infection from a different site in the same individual.