Objective To investigate the clinical efficacy of cobamamide combined with Longxuejie in the treatment of herpes zoster in the elderly .Methods 104 elderly patients with herpes zoster were randomly divided into the treatment group and the control group .Both the two groups were given orally famciclovir 250mg,3 times a day for 14 days,intramuscularly injected gland glycosides cobalamin 500mg,1 day,14 days a course of treatment ,the treat-ment group was given Longxuejie enteric-coated tablets 4,3 times a day orally for 14 days.The blisters disappeared , pain relief,and recovery time of completely crusted ,treatment efficacy and neuralgia were compared between the two groups.Results In the treatment group,34 cases were cured,effective in 13 cases,total effective rate was 90.4%, which was significantly higher than 73.1% in the control group,the difference was statistically significant (χ2 =5.216,P<0.05).The blisters disappeared,pain relief,and recovery time of completely crusted in the treatment group were significantly shorter than those in the control group (t =3.216,5.432,4.643,3.654,all P <0.05).No postherpetic neuralgia was observed in the treatment group ,the incidence rate of postherpetic neuralgia of the control group was 13.5%,the difference was statistically significant (χ2 =4.875,P<0.05).Conclusion The Longxuejie combined with cobamamide in the treatment of herpes zoster in elderly patients has reliable effect ,fast improvement of clinical symptoms,a low incidence of postherpetic neuralgia ,it is worthy of clinical application .

Objective To explore the expressions and significances of sICAM-1 and sCD44v6 in peritoneal lavage fluid after laparoscopically assisted radical gastrectomy,in order to evaluate the risk of laparoscopically assisted radical gastrectomy for the peritoneal plant transfer.Methods Ninety-two patients with gastric cancer were randomly divided into the control group and the experimental group.The control group was treated with open laparotomy and the experimental group was treated with laparoscopic surgery.The concentrations of both sICAM-1 and sCD44v6 were detected by enzyme linked immunosorbent assay.The positive rates of tumor cells in the two groups were compared.Results The expressions of sICAM-1,sCD44v6 in the two groups before operation had no significant difference (t =0.198,P =0.543 ; t =0.897,P =0.429).After operation,sICAM-1 and sCD44v6 levels increased significantly,but there was no significant difference between the two groups (t =0.766,P =0.312 ; t =1.092,P =0.129).The positive rates of tumor cells in the two groups before and after operation have no significant differences (x2 =0.789,P =0.287 ; x2 =0.912,P =0.198).Conclusion Laparoscopically assisted radical gastrectomy does not increase the expression of sICAM-1 and sCD44v6 in peritoneal lavage fluid and the risk of peritoneal metastasis.It is worthy of clinical poptdarization and application.

Objective To observe the auto-limbal epithelium and amniotic membrance transplantation in treatment of Symblepharon.Methods Transplant with auto-limbal epithelium and aminiotie membrance including 23 patients (31eyes) some of them are recurrentcd pterygium,most of them are symblepharons.[ Result ] In the followup period for 3～12 months,Ocular surface reconstruction was successful in 26 of 31 eyes with symb|epharon,but five of them recurrented three months later with lees than pre-transplantation in degree.Conclusion The transplantation with auto-limbal epithelium and membrance transplantation is an effective method for the symblepharon.

This paper describes the design of the peer education program for middle school students’ prevention of tuberculosis. It includes program implementation plan,selection and training of peer educators,format,content and evaluation of peer education,etc,all of which were used for quality assurance of peer education.

Objective:To study the status quo of quality of life and its influencing factors among Junior High School Students of Three-Gorges Reservoir and Non-Three-Gorges Reservoir in Chongqing,and to provide basis for students' QOL improvement.Methods:672 Three-Gorges Reservoir students and 630 Non-Three-Gorges Reservoir students were tested by using the Quality of Life Scale for Junior High School Students(JHSS-QOLS).Multiple stepwise regression analysis was utilized to study the major factors affecting QOL of students in Chongqing.Results:The quality of life of junior high school students in Three-Gorges Reservoir was higher than that of students in Non-Three-Gorges Reservoir.Physical exercises,sleep,chronic disease,parental rearing style were important influencing factors in quality of life of students.Conclusions:The key steps of improving the quality of life of Junior High School Students should include reducing the learning load of students,the prevention and control of chronic disease and the improvement of parental educational level.

Objective To compare the sensitivity of body mass index (BMI),waist-hip ratio (WHR)and percentage body fat (PBF)in the evaluation of the obese adolescents population,and find a more sensitive evaluation method.Methods In this study,2 045 adolescents were recruited in the city of Chongqing by using multiple-stage random cluster sampling,and they were identified as overweight or obesity on the base of BMI,WHR and PBF.Results There was significant difference among the detection rate of the three methods (P<0.05),the detection rate of PBF was the highest (12.9%),then BMI (7.2%)and WHR (6.0%).consistency test showed that the concordance of three methods was poor and Kappa (BMI and WHR)=0.199,Kappa (BMI and PBF )=0.441,Kappa (WHR and PBF)=0.187;comprehensive evaluation was chosen as the gold standard and compared with three meth-ods respectively by ROC curve,the analysis showed that area under curve (AVC):PBF(0.954)>BMI(0.918)>WHR (0.694). Conclusion The sensitivity of PBF and BMI was higher.Although the WHR sensitivity was lower,WHR is a good predictor asso-ciated for chronic diseases,which suggests that the development of appropriate adolescent WHR standard is necessary.

Objective:To study the clinical value of susceptibility weighted imaging(SWI)in brain diseases.Methods:37 cases with blood vessel pathological change of brain diseases were collected and analyzed,including 8 example of angioma cavernosum,10 cerebral infarction,15 brain tumor,2 venous malformation and 2 venous sinus thrombus.Conventional T1WI,T2WI,DWI,the SWI and enhanced T1WI,MRA images were performed to compare the superiority of the SWI images to the others in demonstrating the small hemorrhagic focuses,the small vein,hemosiderin,calcification and other paramagnetic materials.Results:SWI could distinguish the hemorrhage from the blood vessel and discover the smaller hemorrhagic focuses of the angioma cavernosum.It could discover more tiny veins adjacent to the dilated draining vein.SWI could detect small hemorrhages in acute cerebral infarction and small draining veins in brain tumor.And draining veins in deep part and expanded rete venosum in the surfaces of brains could be obviously demonstrated when sinus venosus thrombus occurred.Conclusion:SWI is a new pulse sequence that can demonstrate the abnormal low current capacity blood vessels,the structure of small vein,the tiny hemorrhages as well as ferrugination and calcium deposition sensitively.So it can be applied in the diagnosis and antidiastole of the central nervous system diseases as an important supplement to the MRI conventional sequences.

AIM: To study the mechanism and protective effect of rosmarinic acid from Rosmarinus officinalis extract on acute hepatic injury induced by carbon tetrachloride in mice. METHODS: Mouse model of acute hepatic injury was set up by giving CCl_4 abdominally.The effects on ALT,AST avtivities,TG level in serum and MDA level in liver tissue were examined.Histological change and pathological change were observed by means of hematoxylin and eosin staining.The body weight,liver weight of each mice were measured. RESULTS: Rosmarinic acid significantly reduced the activities of ALT,AST,level of TG in serum and level of MDA in liver tissue,restrained the enlargement of liver and reduced the necrosis in pathological observation. CONCLUSION: Rosmarinic acid shows significant inhibitory effects on liver injury induced by CCl_4 in mice.The mechanisms may be related to its effects of reducing lipid peroxidation product.

Objective:To investigate the influences of different OD value and centrifugal parameters on the ultrastructure of Escherichia coli in order to find out the best OD value,speed and time of centrifugation.Methods:According to orthogonal experimental designing principle,the E.coli with different OD600 values are divided into 9 different groups of centrifuge speed and time.The changes of E.coli morphology in each group at different OD600 value,centrifuge time and speed were compared under transmission electron microscope.Results:The different OD600 values and centrifugal parameters have little influence on the ultrastructure of E.coli,but when the OD600 value at 0.8,centrifuge time at 15 min,the cross-section ratio was significantly higher than other groups and was more suitable for observation.Conclusion:The best parameter to prepare the TEM samples of E.coli is under the OD600 value at 0.8 and centrifugation time at 8 minutes and speed at 5 000～12 000 r/min.

Objective: To investigate the effect of two modes of one-lung ventilation(OLV) on lung lobectomy in pediatric patients.Metheds: Twenty ASA Ⅰ-Ⅱ patients(8 male,12 female) aged 2~14 year,weighing 10~30 kg,and undergoing lung lobectomy were enrolled in this study.The patients were premedicated with intramuscular Sod Luminal 3~5 mg/kg and Atropine 0.01 mg/kg im,30 minutes before induction.Induction was performed with Dorcum 0.1 mg/kg,Fentanyl 5 ?g/kg and Vecuronium 0.1 mg/kg,maintained with Vecuronium 70 ?g/(kg?h) and Propofol 5mg/(kg?h) iv boluses.Ordinary tracheal tube was inserted into bronchus(14right,6 left) during OLV.First,OLV with volume-control mode maintained 25 min,and then,OLV with pressure-control mode was performed.In first step Vt was set at 8~10 ml/kg and respiratory rate was adjusted to maintain PetCO2 between 30~40 mmHg.In second step the inspiratory pressure was set according to the plateau pressure during step 1,hemodynamics and PaO2 were measured and recorded.Results : During OLV with pressure-control mode PaO2 was significantly increased,as compared with that during volume-control mode(P