1.Expression of VEGF and PD-ECGF, and Proliferative Activity of Ki-67 according to Clinicopathologic Feature in Cervical Tumor.
Myung Gi LEE ; Tae Bon KOO ; Il Soo PARK
Korean Journal of Gynecologic Oncology and Colposcopy 2000;11(3):290-300
OBJECTIVE: The objective of this study is to evaluate the expressions, microvessel counts and angiogenic pathway of VEGF and PD-ECGF and proliferative activity of Ki-67 according to clinicopathologic feature of cervical tumor. METHODS: Two hundred three cervical specimens were evaluated; among these 20 were designated normal epithelium, 36 mild dysplasia, 28 moderate dysplasia, 36 severe dysplasia, 28 carcinoma in situ, 17 microinvasive carcinoma and 38 invasive cervical carcinoma (21 squamous cell carcinoma and 17 adenocarcinoma). Microvessel count was determined by immunohistochemical staining using anti-factor VIII-related monoclonal antibody. The expression of VEGF (vascular endothelial growth factor) and PD-ECGF (platelet-derived endothelial cell growth factor) were evaluated by immunohistochemical staining with anti-human VEGF monoclonal antibody and anti-dThdPase monoclonal antibody. The proliferative activity was examined using a Ki-67 equivalent monoclonal antibody (MIBl). RESULT: There was no statistical significance on microvessel count except invasive cancer comparing with mild dysplasia including normal tissue, but there was a little increase in microvessel counts according to severity of tumor. The intensity of VEGF and PD-ECGF expression was significantly correlated with severity of cervical tumor. And the microvessel density was significantly higher in the positive expression of VEGF and PD-ECGF than in the negative expression. The intensity of PD-ECGF expression in invasive adenocarcinoma was significantly lower in comparison with VEGF expression. The intensity of Ki-67 expression had no correlation with severity of cervical tumor and was significantly higher in moderate and severe dysplasia than in microinvasive and invasive carcinoma. Ki-67 expression had no statistical correlation with VEGF and PD-ECGF. CONCLUSION: The VEGF and PD-ECGF are important angiogenic factors and associated with progression of cervical tumor. The VEGF may be involved in the progressions of squamous cell carcinoma and adenocarcinoma, but the PD-ECGF may not be involved or be minimally involved in the progression of adenocareinoma. There seems to be a different angiogenic pathway pertaining to the histologic difference of cervical cancer. There was no difference of Ki-67 expression according to severity of cervical tumor.
Adenocarcinoma
;
Angiogenesis Inducing Agents
;
Carcinoma in Situ
;
Carcinoma, Squamous Cell
;
Endothelial Cells
;
Epithelium
;
Microvessels
;
Thymidine Phosphorylase*
;
Uterine Cervical Neoplasms
;
Vascular Endothelial Growth Factor A*
2.Cytogenetic Analysis of 467 Cases of Amniocetesis.
Soon Ku HWONG ; Soo Min SON ; Jung Gi LEE ; Myung Gi LEE ; Yong Chul BAE ; Yong Tae HAN
Korean Journal of Perinatology 1999;10(2):189-193
OBJECTIVE: The study of 467 cases of amniocentesis have been done at the department of Genetics, Taegu Cheil Hospital from Oct. 1997 to May 1999 for the purpose of analysis of abnormal karyotype according to the indication and age distribution, METHODS: We collected amniotic fluid using 22G spinal needle and measured amniotic alphafetoprotein and acetylcholine esterase in supematant and performed cytogenetic analysis. RESULTS: Positive Down screeing(positive triple test) was the most common indication of amniocentesis (61.5%) and abnormal karyotypes were 24 cases(5.1%) in 467 cases. Among 24 abnormal cases, 10 cases(2.1%) of 21 trisomy were observed. Abnormal karyotypes were most common in the group of abnormal ultrasonogram finding and the gmup of maternal age between 31 to 35 years old, which consists of 25% and 7.7% respectively. CONCLUSION: More attention for the abnormal karyotype should be paid to the group of abnormal ultrasonogram finding and the group of maternal age between 31 to 35 years old as well as above 35 years old.
Abnormal Karyotype
;
Acetylcholine
;
Adult
;
Age Distribution
;
Amniocentesis
;
Amniotic Fluid
;
Cytogenetic Analysis*
;
Cytogenetics*
;
Daegu
;
Female
;
Genetics
;
Humans
;
Karyotype
;
Maternal Age
;
Needles
;
Trisomy
;
Ultrasonography
3.Clinical Efficacy and Tolerability of Terbinafine 1% Cream in Patients with Pityriasis Versicolor.
Ho Sun JANG ; Hyun Tae LEE ; Cheon Gi KIM ; Kyung Sool KWON ; Tae Ahn CHUNG
Annals of Dermatology 1996;8(3):169-176
No abstract available.
Humans
;
Pityriasis*
;
Tinea Versicolor*
;
Treatment Outcome*
4.The Effect of EDTA and Fetal Cord Serum Supplementation to Ham's F-10 Culture Medium on Development Potential of Mouse Embryos in Vitro.
Byeong Seog KIM ; Young GI LEE ; Yoon Kee PARK ; Tae Hyung LEE ; Sung Ho LEE
Yeungnam University Journal of Medicine 1995;12(1):124-134
It is the most important to select optimal culture conditions to promote safe embryo growth in the technique of human in vitro fertilization and embryo transfer. It has been shown that the addition of biologic fluids, such as blood serum, of various origins, improved fertilization and early cleavage rates in numerous species. The purpose- of this study is to attempt to measure developmental potential of mouse eggs fertilized and cleaved in Ham's F10 culture medium containing a chelating agent, EDTA and fetal cord serum. hi this study, we selected 40 female mice and 20 male mice, and investigated optimal serum concentration' for mouse embryo growth. Two cell stage mouse embryos were cultured in Ham's F-10 medium, Ham's F-10 medium with various concentrations of EDTA, or Ham's F-10 medium with EDTA and 10% human cord serum. Developmental ratios to morula in Ham's F-10 medium containing various concentrations of EDTA and/or 10% fetal cord serum were significantly higher than in unsupplemented Ham's F-10 medium (p<0.05). Developmental ratios to blastocyst in Ham's F-10 containing 10% fetal cord serum and 5011M or 100pM EDTA were significanldy higher than in unsupplemented Ham's F-10 medium (p<0.05). Developmental ratios to morula in Ham's F-10 containing 10% fetal cord serum and 100,uM EDTA were significanitly higher than in Ham's F-10 with 10% fetal cord serum used commonly in many human IVF centers(p<0.05). Developmental ratio to blastocyst in Ham's F-10 containing 10% fetal cord serum and 100pM EDTA was significanity higher than in Ham's F-10 with 200pM EDTA(p<0.05).
Animals
;
Blastocyst
;
Edetic Acid*
;
Eggs
;
Embryo Transfer
;
Embryonic Structures*
;
Female
;
Fertilization
;
Fertilization in Vitro
;
Humans
;
Male
;
Mice*
;
Morula
;
Ovum
;
Serum
5.Intrageneric Relationships of Trichoderma Based on Internal Transcribed Spacers and 5.8S rDNA Nucleotide Sequences.
Gi Young KIM ; Goang Jae LEE ; Myung Gyu HA ; Tae Ho LEE ; Jae Dong LEE
Mycobiology 2000;28(1):11-16
The nucleotide sequences of the internal transcribed spacer (ITS) regions of the ribosomal DNA including the 5.8S ribosomal RNA gene (rDNA) have been determined for 11 species in order to analyze their intrageneric relationships. The total length of these sequences ranged from 530 nucleotides for Trichoderma reesei KCTC 1286 to 553 nucleotide for Trichoderma koningii IAM 12534. Generally speaking, the length of ITS1 region was about 30 nucleotides longer than that of the ITS2 region. Also, the sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites were also found. Thus, a neighbor-joining tree was constructed using the full sequence data of the ITS regions and the 5.8S rDNA. The Trichoderma genus used to be grouped on the basis of the morphological features and especially the shape of phialides needs to be reexamined. The phylogenetic tree displayed the presence of monophylogeny in the species of Trichoderma. Therefore, it was difficult to distinguish the intrageneric relationships in the Trichoderma genus.
Base Sequence*
;
DNA, Ribosomal*
;
Nucleotides
;
Phylogeny
;
RNA, Ribosomal, 5.8S
;
Trichoderma*
6.The Formation of Giant Mitochondria in the Liver Cells Induced by Hydrazine.
Il Hoon KWON ; Jong Gi LEE ; Yoon Kyung SOHN ; Tae Joong SOHN
Korean Journal of Pathology 1986;20(3):288-294
The authors studied the formation of giant mitochondria in liver cell. The Sprague Dawley rats were sacrificed following intervals; 5, 10, 20, 30, and 60 minutes after intraperitoneal injection of hydrazine in the amount of 200 microliter/kg. And the extracted liver tissues were examined with light and electron microscopes. The results obtained were summarized as follow; Light microscopically, there is little difference between control and experimental groups. Electron microscopically, elongated, bizzare shaped mitochondria are appears 5 minutes after hydrazine injection. Those show attenuated portion, Y, U, or C shaped feature suggesting fusion or budding mitochondria. The number of giant mitochondria is decreased after 10 minutes group and rarely present in 60 minutes group. The results suggest in this experiment that the formation of giant mitochondria is kind of reversible change and it is different from the mitochondrial swelling of cellular injury. Intermitochondrial fusion and mitochondrial budding may be related with the formation of giant mitochondria.
Rats
;
Animals
7.Malignant Histiocytosis: A case report.
Sook Tae HA ; Gi Yeong HUH ; Kang Suek SUH ; Sun Kyung LEE
Korean Journal of Pathology 1986;20(3):395-398
We have recently experienced a clinically atypical case of malignant histiocytosis involving the spleen, ileum and bone marrow, in a 25-year-old Korean male. He was admitted to our hospital because of multiple purpuric lesions of skin which had been appearing since 2 years ago. Physical and laboratory examinations revealed marked splenomegaly with pancytopenia. With clinical impression of hypersplenism, splenectomy was performed. During the operation, the surgeons found a dark red mucosal lesion in the ileum incidentally, to resect the segment. Microscopic findings of the spleen and the ileal lesion showed extensive proliferation of atypical histiocytes, many of them phagocytosing numerous erythrocytes. Bone marrow biopsy was done for follow-up study, which showed increased cellularity with infiltration of atypical histiocytes.
Follow-Up Studies
;
Biopsy
8.A Clinical Study on Rhegmatogenous Retinal Detachment.
Seung Gi KIM ; Kuhl HUH ; Tae Soo LEE
Journal of the Korean Ophthalmological Society 1995;36(5):793-800
We reviewed charts of patients on the clinical characteristics and factors which affect the prognosis of surgical results in the 77 patients(77 eyes) who were diagnosed with rhegmatogenous retinal detachment and operated on and followed for at least 3 months postoperatively at Korea University Guro hospital. We evaluated the characteristics of rhegmatogenous retinal detachment in Korea by comparing with other reports on retinal detachment as well. The factors associated with anatomic success were the duration of retinal detachment and the prescence of proliferative vitreoretinopathy. High success rate was achieved when the duration was less than 1 month and there was no proliferative vitreoretinopathy. The factors associated with functional success included the duration of retinal detachment, proliferative vitreoretinopathy, preoperative refractive error and the degree of retinal detachment(P<0.05).
Humans
;
Korea
;
Prognosis
;
Refractive Errors
;
Retinal Detachment*
;
Retinaldehyde*
;
Vitreoretinopathy, Proliferative
9.Mycobacterium Fortuitum Infection of Acupuncture Sites.
Joon Ho LEE ; Hyoung Gi CHA ; Doo Chan MOON ; Kyung Sool KWON ; Tae Ahn CHUNG
Annals of Dermatology 1994;6(1):69-73
We report a case of Mycobacterium(M.) fortuitum infection in a 65-year-old female who presented with erythematous to purplish colored tender nodules and plaques with curst and purulent discharge on both upper and lower extremities along the sites of acupuncture. The culture of surgically excised specimen in 3% Ogawa media yielded slightly yellowish colored colonies within 2 days. Several tests for identification of the species were performed and growth on 5% NaCl, negative niacin test, positive results in nitrate reduction, catalase, urease and iron uptake tests were noted. Excision of the lesions followed by administration of minocycline and ciprofloxacin showed no sign of relapse to data a year after treatment.
Acupuncture*
;
Aged
;
Catalase
;
Ciprofloxacin
;
Female
;
Humans
;
Iron
;
Lower Extremity
;
Minocycline
;
Mycobacterium fortuitum*
;
Mycobacterium*
;
Niacin
;
Recurrence
;
Urease
10.Familial Atrophoderma Vermiculata Associated with Epidermal Cysts.
Young Gi KIM ; June Woo KIM ; Mi Kyeong KIM ; Ji Yeoun LEE ; Tae Young YOON
Annals of Dermatology 2005;17(2):102-105
No abstract available.
Epidermal Cyst*