The purpose of this paper is to present the research on the molecular biological characteristics of proto-oncogene pim-2 and to analyze the related mechanism. Proto-oncogene pim-2 was studied and analyzed by the bioinformatics method and technology. With an online server, the chromosomal localization of pim-2 gene was analyzed, and the exon, open reading frame, CpG island and miRNAs complementary fragments and the like were predicted. With bioinformatics software, the physicochemical property of transcription protein of proto-oncogene pim-2 and various modification sites of protein sequence, such as ubiquitination and glycosylation, were predicted, the antigenic index was calculated, and the spatial structural was modeled. The research findings showed that the proto-oncogene pim-2 comprised six exons, the CDS (coding sequence) transcribed a section of peptide chain including 311 amino acids, a gene promoter has a CpG island, and the 3'UTR region contains an miRNA gene. The molecular weight of the Pim-2 protein was 34,188. 47, the isoelectric point was 5.78, the instability index was 45.87, and the extinction coefficient was 279nm. A plurality of covalent modification sites, two ubiquitination sites, four glycosylation sites, an SUMO sumoylation site, a nitrosation site, two palmitoylation sites and sixteen regions with higher antigenic index were distributed in the protein sequence. This research showed that the related regions and modification sites distributed on the sequence of proto-oncogene pim-2 were closely related to the carcinogenic effect thereof.
Amino Acid Sequence ; CpG Islands ; Exons ; Open Reading Frames ; Protein-Serine-Threonine Kinases ; genetics ; Proto-Oncogene Proteins ; genetics

Objective To assess the teaching effect of "paper type test report" teaching mode in medical laboratory specialty undergraduate class. Methods In the teaching of "Clinical Immunology Examination" and "Clinical Thera-peutic Drug Monitoring Technology", the medical laboratory specialty undergraduate class of Grade 2009 were divided into test group(n=28) and control group(n=27)at random,"paper type test report"and traditional teaching modes were applied respectively. The teaching effects were evaluated by experiment examination result, practice result, graduation thesis and class theory achievement, etc. Results Graduation thesis scores of experimental group were significantly bet-ter than that of the control group (P<0.05). In the teaching of"the Clinical Therapeutic Drug Monitoring Technology", the experimental results, the experimental results, the theory exam overall rating scores were better than control group, and the differences were statistically significant (P<0.05). Conclusion Teaching model of "paper type test re-port"plays a positive role in improving of students' writing ability and consolidating of theory knowledge ,etc.