No abstract available.
Cervix Uteri* ; Female ; Health Expenditures*

Six species of the genus Laboulbenia were collected from some regions of Yunnan Province, China, in July, 1994. They are as follows; Laboulbenia acupapi Spegazzini was collected on Abacetus submetallicus (Nietner) and Stenolophus iridicolor Redtenbacher. The host of this fungus has been only found on genus Acupalpus in the world so far, but it was newly found on genus Abacetus and Stenolophus. Laboulbenia anoplogenii Thaxter subsp. abaceti Santamaria was collected on Abacetus submetallicus (Nietner). Laboulbenia elaphri Spegazzini was collected on Nebria ochotica Sahlberg, but the host of this fungus has been only found on genus Elaphrus in the world so far. It was newly collected on genus Nebria. Laboulnebia flagellata Peyritsch was collected on Harpalus platynotus Bates and Platynus sculptipes (Bates). Laboulbenia polyphaga Thaxter was collected on Harpalus bungii Chaudoir. Laboulbenia vulgaris Peyritsch was collected on Bembidion misellum Harold and Bembidon scopulium (Kirby).
Beetles ; China* ; Fungi

BACKGROUND: For the diagnosis of malaria, examination of blood smear slides by light microscopy is used as standard, and commercial kits detecting malarial antibodies and antigens are available, and molecular methods such as polymerase chain reaction (PCR) are used additionally. But, these diagnostic methods can be performed when clinicians request them, so problems of misdiagnosing the patients who are not suspected malaria may be occurred. METHODS: In 42 Korean patients with malaria, the author analyzed the characteristic signals of malaria using granularity (90 degrees depolarized) versus lobularity (90 degrees polarized) graph of Cell-Dyn 4000 (CD4000) automatic hematologic analyzer. And, the author examined the presence of malaria in 421 random samples by CD4000 and Giemsa stain. RESULTS: The usefulness of CD4000 in diagnosing malaria are as follows, 93.0% sensitivity, 99.3% specificity, 93.0% positive-predictive value, and 99.3% negative-predictive value. CONCLUSION: CD4000 automatic hematology analyzer has high diagnostic sensitivity and specificity in diagnosing malaria. Because complete blood count (CBC) is the routine test for most patients, this method has advantage of time and cost effectiveness and can even detect malaria in unsuspected cases.
Antibodies ; Azure Stains ; Blood Cell Count ; Cost-Benefit Analysis ; Diagnosis* ; Hematology* ; Humans ; Malaria* ; Microscopy ; Polymerase Chain Reaction ; Sensitivity and Specificity

OBJECTIVES: This study was conducted to assess the development of posttraumatic stress disorder (PTSD) after occupational physical injuries and the risk factors in occupational accident patients for PTSD. METHODS: Forty-seven occupational accident patients with physical injuries were administered BDI, STAI I and II, SCL-90-R, IES and questionnaires for risk factors and Clinician-Administered PTSD Scale(CAPS). RESULTS: 1) 12 patients(25%) were diagnosed as PTSD. 2) The BDI, STAI I and II and IES scores are significantly high(<0.05) in PTSD group than non-PTSD group. 3) The subscales of SCL-90-R, with an exception of PAR subscale, showed higher scores(<0.05) in PTSD group. 4) The loss of consciousness(LOC) was only significant risk factor in the occupational accident-related factors(<0.05). CONCLUSION: The results of this study show the development of PTSD among occupational accident patients. The loss of consciousness(LOC) was the only signiticant risk factor for PTSD in occupational accident patients.
Accidents, Occupational* ; Humans ; Surveys and Questionnaires ; Risk Factors ; Stress Disorders, Post-Traumatic*

BACKGROUND: Breast lesions in children and adolescents are rare and develop mainly in females. The management of breast masses in children and adolescents can be a perplexing problem for pediatricians and surgeons. It is well documented that breast lesions in females under 20 years of age rarely represent malignant disease. However, they frequently are a source of discomfort and anxiety for the patient, particularly when they do not resolve spontaneously. METHODS: To determine the frequency of different breast masses in females under 20 years of age on whom surgery was performed, we carried out a retrospective study of females under 20 years who were admitted to the Department. of Surgery of Soonchunhyang University Hospital for surgery between 1993 and 1995. RESULTS: The mean age was 17.9 years. A total of 44 surgical procedures were performed in 40 patients. The masses ranged in size from 0.8 cm through 7.0 cm. As to the location, the mass was in the right breast of 22 patients (50%) and in the left breast of 22 (50%). The most common location of the mass was in the right upper outer quadrant (13 patients, 29.5%). Of the 44 masses, fine-needle aspiration cytology was performed in 33 masses. Of them, 28 were fibroadenomas, 4 were fibrocystic disease, and 1 was a lipoma. Excisional biopsy was performed on 44 masses. Of them, 33 were fibroadenomas, 4 were fibrocystic disease, 5 was fibroadenoma and fibrocystic disease, 1 was a lipoma, and 1 was a lymphangioma. There were no malignancies. CONCLUSIONS: In female patients under 20 years of age, we suggest that benign breast should be studied for diagnosis, treatment, and histological feature.
Adolescent ; Anxiety ; Biopsy ; Biopsy, Fine-Needle ; Breast* ; Child ; Diagnosis ; Female* ; Fibroadenoma ; Humans ; Lipoma ; Lymphangioma ; Retrospective Studies

No abstract available.
Aged ; Antineoplastic Combined Chemotherapy Protocols/adverse effects/*therapeutic use ; Blood Cells/pathology ; Bone Marrow Cells/pathology ; Carcinoma, Non-Small-Cell Lung/*drug therapy/radiotherapy ; Humans ; Karyotyping ; Leukemia, Megakaryoblastic, Acute/*diagnosis/etiology ; Lung Neoplasms/*drug therapy/radiotherapy ; Male

BACKGROUND: Rapid culture and identification of mycobacteria is important to effective treatment and management of patient. We made in-house biphasic media and designed identification system by multiplex PCR/PCR-RFLP. We tried to compare the report time of new system with that of conventional one. METHODS: A total of seven hundred and forty specimens sent for tuberculosis culture in a tertiary care hospital were inoculated into biphasic media which was composed of Ogawa media and Middlebrook 7H9 broth. When growth was detected, Mycobacterium tuberculosis and nontuberculous mycobacteria were identified by cord formation in AFB stain and multiplex PCR using IS6110 and hsp65 gene. Diagnostic algorithm for mycobacteria was established by PCR-RFLP with 13 species, 58 mycobacteria. We tried to identify 14 M. tuberculosis and 18 nontuberculous mycobacteria by that system. RESULTS: Eighty-four mycobacteria (11.4%) out of 740 specimens isolated. The mean report time of new system was 21+/-8.2 day, while that of conventional one was 31+/-8.3 day (P<0.005). Algorithm capable of identification 13 mycobacteria species was made. It showed 19 RFLP patterns and 7 of them were new ones which have not been described. The result of identification for 32 mycobacteria by PCR-RFLP was identical with that of biochemical method. CONCLUSIONS: Culture and identification of mycobacteria by biphasic media and Multiplex PCR/PCR- RFLP was a easy, rapid and cost-effective method in the area with the high prevalence rate of tuberculosis.
Humans ; Multiplex Polymerase Chain Reaction ; Mycobacterium tuberculosis ; Nontuberculous Mycobacteria ; Polymorphism, Restriction Fragment Length ; Prevalence ; Tertiary Healthcare ; Tuberculosis

BACKGROUND: Because of the power of HRCT to detect the minute changes in lung parenchyme and the advantage of noninvasiveness, it may be the ideal method of follow-up evaluation of the patients with diffuse interstitial pulmonary fibrosis. So the aim of the study is to find out whether the change of HRCT during the course of the disease can represent the change in symptom and pulmonary function rest. METHODS: Thirteen patients with diagnosis of diffuse interstitial pulmonary fibrosis who had more than two times of HRCT during the course of disease were studied. The extent of the disease in HRCT was measured at three levels using image analyzer The ralationship between the percent change in the disease extent in HRCT, symptom score, and pulmonary function testis were analysed. RESULTS: The change of disease extent in HRCT has good correlation with the change in dyspnea and diffusion capacity(r=0.716, p=0.0012). But there was no correlation between the change of HRCT and FVC or TLC. Also significant inverse correlation was noted between the change in dyspnea and diffusion capacity(r=-0.707, p=0.0047). CONCLUSION: These data suggest that HRCT may be a good method of follow-up in diffuse interstitial pulmonary fibrosis.
Diagnosis ; Diffusion ; Dyspnea ; Follow-Up Studies* ; Humans ; Lung ; Pulmonary Fibrosis* ; Testis

BACKGROUND: The cryopreservation of Red Blood Cells have been proved to have many advantages in western countries. However, in Korea, clinical application of frozen-thawed blood is still in its early stage. We tried autologous frozen-thawed blood transfusion and evaluated the effects and complications. METHODS: Nine units of whole blood were collected from the autologous (n=1) and allogeneic bone marrow donors (n=3) and made packed RBCs by a centrifugation method. We made frozen RBCs using high glycerol method and stored them in -80degrees C freezer for 1 month. Eight units of frozen RBCs were thawed and washed by Cobe spectra (Cobe, USA). Autologous transfusions were done to the patient and donors while the bone marrow collection procedures were in process for bone marrow transplantation and we carefully observed its effects and complications. RESULTS: The mean RBC recovery rate were 89.8% and the supernatant plasma hemoglobin, K+, LD, osmolality, and simulation test were satisfactory to the allowable limit. After transfusion, the hemoglobin value was significantly elevated being comparable to that of the fresh blood. A case showed mild hematuria related to this transfusion but soon it disappeared in a day. CONCLUSION: The autologous frozen-thawed blood transfusions were safe and as much effective as the fresh blood. It would be essential to have skillful techniques in post-thaw washing process.
Blood Transfusion* ; Bone Marrow Transplantation ; Bone Marrow* ; Centrifugation ; Cryopreservation ; Erythrocytes ; Glycerol ; Hematuria ; Humans ; Korea ; Osmolar Concentration ; Plasma ; Tissue Donors*

BACKGROUND: Invasive fungal infections (IFIs) are a life-threatening problem in immunocompromised patients. Despite timely diagnosis and appropriate antifungal therapy, clinical outcomes of IFIs remain unsatisfactory, necessitating treatment with a combination of antifungal agents. Therefore, childhood leukemic patients treated with voriconazole plus caspofungin were evaluated for the safety and efficacy of the combination antifungal therapy to treat IFIs. METHODS: In this retrospective study, medical records were retrieved for patients admitted to the Pediatric Department of Yeungnam University Hospital, Daegu, South Korea, between April 2009 and May 2013. Medical records of 22 patients were analyzed. RESULTS: Of the 22 patients studied, nine (41%) had been diagnosed with probable IFI, and 13 (59%) with possible IFI. All patients, except one, were already receiving antifungal monotherapy for the treatment of neutropenic fever. After a diagnosis of IFI was confirmed, antifungal monotherapy was replaced with combination therapy. The study's overall response rate was 90.9%, with complete responses in 86.3% of the patients. Two patients experienced a side effect of a small increase in liver enzyme levels. CONCLUSION: Voriconazole plus caspofungin combination therapy is an effective and safe treatment for serious IFI in pediatric patients with acute leukemia.
Antifungal Agents ; Aspergillosis ; Child* ; Daegu ; Diagnosis ; Echinocandins ; Fever ; Humans ; Immunocompromised Host ; Korea ; Leukemia* ; Liver ; Medical Records ; Retrospective Studies ; Voriconazole*