Dust ; Humans ; Pneumoconiosis ; etiology

Animals ; Antigens, Nuclear ; DNA ; genetics ; metabolism ; DNA Breaks, Double-Stranded ; DNA Repair ; DNA-Binding Proteins ; Humans ; Ku Autoantigen

Genomics ; Proteomics ; Toxicology

Animals ; DNA Damage ; DNA Repair ; DNA-Activated Protein Kinase ; Humans

Apoptosis ; Cell Cycle ; Cell Differentiation ; Cell Proliferation ; Humans ; Neoplastic Processes ; Proto-Oncogene Proteins c-jun ; genetics ; metabolism ; physiology

Attitude to Health ; China ; Dust ; prevention & control ; Humans ; Needs Assessment ; Occupational Exposure ; prevention & control ; Occupational Health Services ; Pneumoconiosis ; prevention & control ; Surveys and Questionnaires

Dust ; Humans ; Occupational Exposure ; prevention & control ; Pneumoconiosis ; prevention & control

China ; epidemiology ; Dust ; prevention & control ; Humans ; Silicon Dioxide ; chemistry ; Silicosis ; epidemiology ; prevention & control

OBJECTIVETo study the roles of Ku80/p53 pathway in silica-induced cell cycle changes in human embryo lung fibroblasts (HELF).

METHODSKu80 siRNA expression vectors were transfected into HELF by lipofectamine. Flow cytometry was used to detect the distributions of cell cycle and western blot assay was used to determine the expression level of Ku80, p53 and p21 proteins or the phosphorylation levels of p53-ser15 after cells were exposed to silica.

RESULTSThe expression levels of Ku80 protein increased in concentration-dependent and time-dependent manners after cells were exposed to silica. The proportion of G1 phases in H-NC cells (controls) decreased from 89.28% +/- 2.19% to 68.93% +/- 3.79% after exposure to silica, and the proportion of G1 phases in HELF cells (H-Ku80) decreased from 85.16% +/- 3.73% to 59.92% +/- 3.31% after exposure to silica (P<0.05). The expression levels of Ku80, p53 proteins or p21 proteins or phosphorylation level of p53-ser15 were obviously suppressed in H-Ku80, as compared with H-NC.

CONCLUSIONKu80/p53 pathway plays a role in the cell cycle charges induced by silica in human embryo lung fibroblasts.

Antigens, Nuclear ; metabolism ; Cell Cycle ; drug effects ; Cells, Cultured ; Cyclin-Dependent Kinase Inhibitor p21 ; metabolism ; DNA-Binding Proteins ; metabolism ; Fibroblasts ; cytology ; drug effects ; metabolism ; Flow Cytometry ; Humans ; Ku Autoantigen ; Lung ; cytology ; metabolism ; Phosphorylation ; Quartz ; toxicity ; Signal Transduction ; Tumor Suppressor Protein p53 ; metabolism

OBJECTIVETo study the roles of DNA dependent protein kinase (DNA-PK)in silica-induced cell cycle changes and expressions of CyclinE and CDK2 in human embryo lung fibroblasts (HELF).

METHODSThe expressions of Ku80 and DNA-PKcs proteins were inhibited by siRNA plasmids, respectively. Flow cytometry was used to detect the distributions of cell cycle and western blot assay was used to determine the expression levels of CyclinE and CDK2 after cells were exposed to 200 microg/ml silica for 0, 3, 6, 12, 24 h.

RESULTSThe proportion of G1 phases in negative control cells decreased from 83.53% +/- 2.24% to 69.11% +/- 3.12% after exposure to silica; the proportion of G1 phases in H-Ku80 and H-PKcs cells exposed to silica decreased from 85.16% +/- 3.73% to 59.92% +/- 3.31% and from 75.06% +/- 2.23% to 58.32% +/- 1.35%, respectively (P < 0.05). The exposure to silica resulted in the increasing protein expression levels of CyclinE and CDK2 in negative control cells, and the expression levels of CyclinE were obviously suppressed in H-Ku80 and H-PKcs as compared with control cells. However, the expression level of CDK2 protein did not change significantly.

CONCLUSIONDNA-PK might play a role in silica-induced alternations of cell cycle and regulate silica-induced overexpression of CyclinE in human embryo lung fibroblasts.

Cell Cycle ; drug effects ; Cells, Cultured ; Cyclin E ; metabolism ; Cyclin-Dependent Kinase 2 ; metabolism ; DNA-Activated Protein Kinase ; genetics ; metabolism ; Fibroblasts ; cytology ; drug effects ; metabolism ; Humans ; Lung ; cytology ; Nuclear Proteins ; genetics ; metabolism ; Oncogene Proteins ; metabolism ; Silicon Dioxide ; pharmacology