Objective To study the effects of γ-aminobutyric acid ( GABA) and sertraline on the CABAA(α) receptor and GABAB receptor of hippocampus neuron in rats of depression induced by acute stress. Methods The cognitive function of male SD (Sprague-Dawley) rats was screened through Y-maze. Then rats were randomly divided into five groups. Except control group,rats were injected intraperitoneally with double-distilled, GABA,sertraline or GABA + sertraline respectively. They were exposed to the forced-swimming stress test,which was to make acute stress model of depression. The space memory function in the maze was measured. Then the number of GABA-like immunoreactive neurons in hippocampus CA1 ,CA3 ,DG areas of rats were investigated with immunohistochemist. Results Compared with the model group,the immobility time in the forced-swimming test and the latency in the maze could be reduced significantly after pretreatment with GABA and sertraline (P < 0.01). The positive cell populations of CA1 ,CA3 region and DG gate region in the model group were significantly reduced compared with the control group (P<0.01). GABA could only improve the positive cell populations of GABAB receptor in CA3 region (P<0.01). In the sertraline group,the positive cell populations in hippocampus were increased obviously more than it in model group(P<0.01). The positive cell populations of hippocampus in the GABA + sertraline group in CA1 ( (82.83±8.72),(78.08±5.67)),in CA3((92.83±9.35),(76.00±3.97)),in the gate of DG( (35.00 ±1.41) ,(33.33±4.36)) increased significantly to the other groups (P< 0.05 or 0.01). Conclusion Ectogenic GABA could improve the sertraline' s effects of antagonizing acute depression effectively and the space memory function,and its mechanism may be involved in strengthening the expression of GABAA(α) receptor and GABAB receptor in hippocampus.

Objective To study the similarities and differences of personality in patients with body-dysmorphic disorder(BDD),obsessive-compulsive disorder(OCD) and ordinary person.As well as to provide information for clinical therapy. Methods All BDD patients( n =29),OCD controls( n =30) and normal controls( n =30) completed Self Rating Scale of Body Image (SRSBI) and Minnesota Multiphasic Personality Inventory (MMPI). Results Ten clinical scales score of MMPI in BDD were found significant higher than normal controls which including hypochondriasis,depression,hysteria, psychopathic deviate, masculinity-femininity, paranoia,psychasthenia,schizophrenia,and social introversion.Four clinical scales[(68.18±8.70),(65.44±8.73),(61.39±9.37),(60.70±12.88)] were significant higher than OCD controls[(61.09±13.29),(58.82±10.26 ),(56.23±9.58),(50.03±12.63)] which including psychopathic deviate,psychasthenia,schiaophrenia and social introversion( P ＜0.05). But hysteria scale was significant lower than OCD controls( P ＜0.01). Conclusion BDD patients have abnormal personality which is more significant than OCD patients. Significant abnormal personality are likely the psychopathology of BDD from which the clinic symptom produced.

Objective To study the effects of γ-aminobutyric acid (GABA) and sertraline on the cognition functions in acute stressed rats.Methods Y-type maze were used to test the cognitive function of Male SD (Sprague-Dawley) rats.They were exposed to the forced-swimming stress test,which was to make acute stress model of depression.Except control group,rats were pretreated with different doses of GABA and/or sertraline,then the time of immobility in the forced-swimming test and latency in the maze were observed individual.Results Compared with the model group[(1404.33±129.46)s] ,GABA could decrease the immobility time obviously in the forced-swimming test [(432.33±187.64) s ＞ (332.50±217.23) s,P ＜ 0.01].Otherwise the immobility time was prolonged with 0.50 g/kg GABA + 10 mg/kg sertraline (1086.17±411.80) s (P ＜ 0.05).Compared with the control group(105.00±32.18)s,the latency in the maze was prolonged remarkably in the model group (179.17±8.54) s(P＜ 0.05).Pretreatment with 10 mg/kg sertraline(100.30±21.01) s or GABA and sertraline groups (69.83±16.89) s,(86.83±13.11) s (P ＜ 0.05 or 0.01) could decrease the latency.Conclusion GABA could play its role of antagonizing acute depression.Meanwhile,pretreatment with appropriate doses of GABA and sertraline not ordy could decrease doses of two drugs but also improve the cognitive function in the acute stress rats.

Objective To explore the protective effects of Acanthopanax senticousus saponins (ASS) on ischemia-hypoxia injury of cortical neuron. Methods The models of cortical neuron damage induced by hypoxia-ischemia (HI) and glutamate (Glu) were established on cultured embryonic cortical neurons. The neurons were randomly divided into HI group, Glu group, ASS group and control group. ASS (50 mg/L) was added into the ASS group before and during neuron damage. The rate of neuronal apoptosis was measured by flow cytometer, nitric oxide (NO) content was determined by Nitrate reductive assay and neuron survival was measured by MTT assay and the release of LDH. Morphologic change of neurons was observed under electron microscopy.Results (1) The cortical neuron survival decreased time-dependently in HI group and reached peak at 8h after hypoxia-ischemia. Glutamate leaded the cortical neuron survival decreasing time-dependently. (2) Compared with the control group, the cortical neuron survival decreased in HI group and Glu group, but the neuron apoptosis, the release of LDH and NO contents increased significantly (all P

Objective To observe the clinical efficacy of transcutaneous electric acupoint stimulation in intervening early-stage swelling limb after breast cancer operation.Method A hundred patients undergone radical mastectomy for breast cancer were divided into a treatment group and a control group, 50 cases in each group. The control group was intervened by ordinary post-operative nursing, while the treatment group was additionally given transcutaneous electric acupoint stimulation. By measuring the circumference of both upper arms, the incidence rate and degree of swelling arm in 2 weeks after operation were compared, meanwhile, the accompanied symptoms such as tightness of skin, pain in upper limb, numbness in upper limb, and heaviness of upper limb were observed.Result The occurrence rate of abnormal upper-limb sensations was 22.0% in the treatment group versus 84.0% in the control group, and the difference was statistically significant (P<0.01). The occurrence rate of upper-limb swelling was 4.0% in the treatment group, versus 24.0% in the control group, and the difference was statistically significant (P<0.01). Conclusion Transcutaneous electric acupoint stimulation can effectively prevent and release the early-stage upper-limb swelling after breast cancer surgery.

With transmission electron microscopy,the endoplasmic reticulum of the parathyroid chief cells in normal and cadmium-treated golden hamsters was observed. After 1 month treatment of cadmium, there were dilated and whorl-shaped rough endoplasmic reticulum in the chief cells. After 3 and 6 months treatment of cadmium, there were concentric membranous body and paired endoplasmic reticulum in the chief cells. The mechanism responsible for the morphological change of the endoplasmic reticulum after cadmium treatment and the possible toxicologic effect were discussed.

Objective To study the effect of clomiphene citrate (CC) and human menopausal gonadotropin (hMG) on the expression of integrins ? 4? 1 during implantation window in endometrium. Methods Endometrium integrin ? 4? 1 expression was detected by immunohistochemistry method in the mid secretory phase of 48 normal natural cycles. CC+hCG or CC+hMG+hCG cycles in 30 polycystic ovary syndrom (PCOS) patients and 48 normal women. Results The expression of endometrial integrin ? 4? 1 was stronger in the natural cycls than those of ovulation induction cycles with CC+hCG or CC+hMG+hCG. So was the pregnancy cycles as compared with the non pregnant cycles. Conclusion The endometrial receptivity in the min luteal phase and pregnant rate declined during ovulation induction by CC+hCG or CC+hMG+hCG in either normal women or PCOS patients.

Objective To observe the clinical efficacy of transcutaneous acupoint electrical stimulation in intervening constipation during chemotherapy in breast cancer patients.Method Ninety breast cancer patients undergone chemotherapy were randomized into a treatment group and a control group, 45 cases in each group. The control group was intervened by conventional nursing care, while the treatment group was additionally intervened by transcutaneous acupoint electrical stimulation. The clinical efficacies were compared between the two groups.Result The total effective rate was 93.3% in the treatment group versus 73.3% in the control group, and the difference was statistically significant (P<0.01).Conclusion Transcutaneous acupoint electrical stimulation can effectively inhibit the incidence of constipation during chemotherapy in breast cancer patients.

ObjectiveTo study the correlation between serum paraoxanase-1 (PON-1) activity and blood lipid and their changes before and after nasal continuous positive airway pressure ventilation (nCPAP) in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS) and to investigate the effect of PON-1 on the occurrence of candiovascular diseases in OSAHS patients.MethodsAccording to the apnea hypopnea index(AHI),86 OSAHS patients were divided into mild group(24 cases,5 ＜ AHI ≤20),moderate group (25 cases,20