1.Early Results of Endoscopic Saphenous Vein Harvesting in Coronary Artery Bypass Grafting
Hiroyuki Seo ; Yasushi Tsutsumi ; Osamu Monta ; Satoshi Numata ; Sachiko Yamazaki ; Shohei Yoshida ; Hirokazu Ohashi
Japanese Journal of Cardiovascular Surgery 2013;42(5):364-368
Recently, with the advent of medical devices and minimally invasive operations, endoscopic saphenous vein harvesting (EVH) in coronary artery bypass grafting has been widely accepted. Although EVH has short-term advantages of less wound morbidity and better cosmetic results compared with open vein harvesting (OVH), several studies have demonstrated that the mid- and long-term patency rate of EVH veins is significantly lower than that of OVH veins, therefore the role of EVH is currently controversial. The purpose of this study was to investigate the early results of EVH compared with the OVH group. Between April 2011 and December 2012, 115 consecutive patients underwent coronary artery bypass grafting (CABG) in our institution. Of these, EVH was performed in 62 patients and OVH in 53. In EVH groups, all 50 patients were men, and mean age was 71.3±7.8 years. A total of 211 coronary anastomoses, 109 SVGs anastomoses were assessed for patency postoperatively by angiography or enhanced computed tomography before discharge. The mean vein harvesting time was 26.0±8.1 min, and the mean number of ostial branch tear was 0.34±0.59. The overall SVG patency rates at discharge were 95.4% in EVH and 92% in OVH, respectively (p=0.24). There was a significant reduction in the incidence of leg wound complications in the EVH group (EVH : 1.6% ; OVH : 13.2% ; p=0.038). In conclusion, the short-term result of EVH was satisfactory. EVH reduces leg wound complications compared with OVH.
2.The preliminary report of occult HBV infection in Vietnamese
Truong Xuan Bui ; Bang Van Nguyen ; Phuong Minh Tran ; Trach Khanh Nguyen ; Quy Tran ; Yoshihiko Yano ; Yasushi Seo ; Yoshitake Hayashi
Journal of Medical Research 2007;47(1):28-32
Background: Vietnam is located in the endemic region of hepatitis B virus (HBV) infection, but no data of occult HBV infection was reported at present. Objectives: To investigate the prevalence of occult HBV infection in different ethnics of people and generations. Subjects and method: 80 voluteers with HbsAg negative from five different ethnics: Kinh, Tay, Mong, Giay and Dao in a Chino \ufffd?Vietnamses border province (Lao Cai) were enrolled in the study. After HBV-DNA was extracted, nested PCR of S gene and of Core-promoter/Pre-core region were used to detect HBV-DNA. Specifying nucleotide sequence was confirmed by direct sequencing. Results:The prevalence of occult HBV infection in population study was very high 73/80 (91,3%) by nested PCR of Core-promoter/Pre-core, significantly more sensitive than nested PCR of S gene (26,3%) (p<0,0001). The prevalence of occult HBV infection was notdifferent between ethnics of people or between children, adults. Conclusion: Occult HBV infection in Vietnamese is very common; however, nationwide further studies should be carried out to confirm this preliminary results and evaluate the impact of occult HBV infection in Vietnam.
Hepatitis B virus
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Hepatitis B Surface Antigens
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3.Clinical characteristics and molecular characteristics of\r\n', u'hepatitis B virus in chronic hepatitis B virus infected patients\r\n', u'with A1899 mutation in precore region\r\n', u'
Truong Xuan Bui ; Ho Thi Thu Pham ; Phuong Minh Tran ; Son Truong Nguyen ; Long Cong Nguyen ; Trach Khanh Nguyen ; Yasushi Seo ; Yoshihiko Yano
Journal of Medical Research 2007;47(2):64-68
Background: Hepatitis B is an infectious illness caused by hepatitis B virus (HBV) which infects the liver of hominoidea, including humans, and causes an inflammation called hepatitis. Objectives: The aim of study is to clarify clinical features and molecular characteristics of HBV in chronic HBV-infected patients with A 1899 mutation. Subjects and method: HBV genotype, HBV-ONA level, HBeAg and anti-HBe in 29 chronic HBV-infected patients were determined by PCR-RFLP, Real-time PCR and ELISA, respectively. Mutations were analyzed by direct sequencing. Results: Mutations in core-promoter/precore regions of HBV genome can suppress HBeAg secretion and stimulate HBV-ONA replication. The prevalence of hepatocel- lular carcinoma (HCc): 10/29, liver cirrhosis (LC) : 15/29 are significantly higher than that in chronic hepatitis (CH) : 4/29 (P < 0.001). HbeAg seroconversion rate in CH (75%) is higher than that in HCC \r\n', u'(40%) and in LC (53.3%), but not significant (P > 0.05). ALT level is the highest in CH and the lowest in HCC \r\n', u'(P = 0.02), 8/10 (80%) HCC patients have normal range of ALT. HBV-ONA level in HCC and in LC is significantly higher than that in CH (P = 0.024). The emerging of A 1899 is often accompanied by C/G1753 mutation (37.9%) and dual core-promoter mutation T1762A1764 (79.3%). Conclusion: A1899 mutation can play a role in the pathogenesis of liver diseases in chronic HBV-infected Vietnamese.\r\n', u'
Hepatitis B virus/ growth &
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development
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physiology
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Hepatitis B
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Chronic/ pathology
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transmission