Objective To inuestigate the clinical efficacy of complete dentures combined with Naoluotong capsule on patients with edentulous . Methods Edentulous patients were given dentures and denture combined with Naoluotong capsule therapy for the treatment.After treatment, chewing efficiency, cerebral blood flow and radiographic were tested and evaluated.Results After 3 months of treatment, satisfaction of combined treatment group was 90.00% which were significantly higher than that of complete denture group(P<0.05); chewing efficiency and cerebral blood flow(Vs、Vm、Vd) results were significantly higher than the denture group (P<0.05);MR studies showed that combined treatment can make the level of activation in the brain regions of patients with missing teeth to the normal value .Conclusion Denture combined with Naoluotong capsule treatment for edentulous patients can get better clinical outcomes and improve brain function of the patients.

Objective To explore the role of MR stem cell markers in temozolomide intervention gliomas angiogenesis and therapy .Methods C6 glioma cells were intravenously injected in glioma model rat.MR stem cell markers and immunohistochemistry were used to observe the tumor growth and angiogenesis.Correlation of contrast imaging and immunohistochemistry results were studied.Results In this study, MR scanning was successfully used to trace the SPIO-PLL marked bone marrow stem cells, dynamic testing of angiogenesis in rat brain glioma tumor.Correlation analysis of imaging and immunohistochemical showed that they had a good negative correlation (P<0.05).Conclusion SPIO magnetic markers for bone marrow stem cells and MR tracing technique is an effective means of dynamic observation in vivo glioma angiogenesis.

Objective To discuss the causes of diseases presenting vertigo as a chief complaint in patients in department of neurology of polyclinic and to compare the clinical features of these disease in order to draw the attention of the physicians to benign paroxysmal positional vertigo(BPPV)and posterior circulation ischenmia(PCI).Methods Prospective clinical study was carried out.Followed up outpatients (135)and inpatients(65)with a chief complaint of vertigo were followed up in the department of neurology, the second affiliated hospital of Harbin Medical University.Thorough history, especially the timing and duration of vertigo, provoking and aggravating factors, symptoms associated and risk factors for cerebrovascular were obtained.Physical examinations with special attention to neurologic systems and Dix- Hallpike maneuver were performed.Based on the history, the physical examination, Dix-Hallpike maneuver and special techniques, the diagnoses of 200 patients with vertigo was made.Two weeks after each patient' s first consultation, a follow-up was made.Results The main diagnoses for these 200 patients were BPPV (56.0%), PCI(21.5%), neurosis(13.0%), M?ni?re' s disease(2.5%), multiple sclerosis (1.0%), probably cervical disease(1.0%), acoustic neuroma(1.0%), labyrinthine apoplexy(1.0%), brainstem hemorrhage(0.5%), vestibular neuronitis(0.5%), cerebellum cyst(0.5%), unknown causes (1.5%).Such clinical features of the diseases with vertigo, as age, vertigo duration, and provoking factors, associated symptoms, risk factors, the physical examinations, the treatment and so on were summed up.Conclusions Presentations of vertigo can be clinically diagnosed in most cases, with a larger number of patients suffering from BPPV.Combination of these prominent clinical features may guide the general physicians to the likely causes of vertigo.

Objective To define the correlation between the proliferating cell nuclear antigen (PCNA) and metastasis-related gene nm-23-H 1, and to correlate their espressions with clinical features,radiosensitivity and prognostic variables in exophageal squamous cell carcinoma (ESCC), to establish some biological parameters obtained prior to therapy though which we can predict radiosensitivity and outcome.Methods PCNA and nm-23-H 1 expression protein were determined by immunohistochemical technique with formalin-fixed paraffin-embedded specimens from 59 patients with ESCC who had received definitive radical radiation and had been followed up for more than 3 years. The values were assessed by distributions of patients , disease factors, including age,sex,lesion site,legth ,histological grade and prognosis.Results The findings showed that the mean labelling indices of PCNA and nm-23-H 1 were significantly higher in ESCC than in the normal esophageal tissue (P0.05).Conclusions PCNA and nm-23-H 1 indices can be taken as biological endpoints to predicting therapeutic response, local and systemic control of disease.

Objective To determine the optimized fractionated radiation schedule by comparing the dose-response relationship between different fractionated radiation schedules with a total dose of 40 Gy or 60 Gy in subclinical breast tumor.Methods Balb/c nude mice bearing subclinical human breast cancer (injected subcutaneously into the hind legs with 1.5 × 105 or 3.1 × 105 exponentially growing MCF-7 cells) were assigned randomly to blank control group (without radiation),conventionally fractionated radiation group (200 cGy,once daily,10 times/week),hyperfractionated radiation group (160 cGy,twice daily with an interval of 6 h,5 times/week),first hypofractionated radiation group (300 cGy,once daily,5 times/ week),and second hypofractionated radiation group (400 cGy,once every other day,3 times/week) ;the total dose was 40 Gy or 60 Gy.The measurement indices were tumor formation rate,short-term tumor control rate,long-term tumor control rate,the time of tumor recurrence,and the maximum diameter of the bottom of tumor.The observation lasted 24 weeks.Data were compared between these groups by chi-square test.Results With a total dose of 40 Gy (the number of injected cells was 1.5 × 105,the tumor formation rate of the blank control group was 2/8),hyperfractionated radiation was the optimized schedule.With a total dose of 60 Gy (the number of injected cells was 3.1 × 105,the tumor formation rate of the blank control group was 11/11),the first hypofractionated radiation (300 cGy,once daily,5 times/week) was the optimized schedule (P =0.001);the short-term and long-term tumor control rates of the conventionally fractionated radiation group,hyperfractionated radiation group,second hypofractionated radiation group,and first hypofractionated radiation group were 0/0 (tumor formation rates:8/8 and 8/8),50％/25％ (tumor formation rates:4/8 and 6/8),25 ％/25 ％ (tumor formation rates:6/8 and 6/8)),and 67 ％/67 ％ (tumor formation rates:4/12 and 4/12),respectively.Conclusions The optimized fractionated radiation schedule for subclinical breast cancer and its total dose vary with the number of injected tumor cells.When the tumor formation rate is 100％,hypofractionated radiation (300 cGy,once daily,5 times/week) is the optimized schedule in terms of long-term tumor control.

Objective To investigate the mechanism of an antifibrotic drug, pirfenidone, in preventing radiation-induced pulmonary fibrosis. Methods Male BALB/C mice were randomized into 4 groups:control group (C), radiation alone group (R), pirfenidone alone group (P), and pirfenidone plus radiation group (P + R). Irradiation was administrated to the whole pulmonary with a single fraction of 12 Gy. The pirfenidone was given 0. 3 ml/kg/d from 3 days prior to irradiation to 12 weeks after.Bronchoalveolar lavage fluid (BALF) from right lung was collected for macrophages counting every monthmonthly until 6 months after irradiation, and left lungs were collected and fixed for. The pulmonary fibrosis was assessed by Masson trichrome staining. The plasma transforming growth factor β(TGF-β) was measured by ELISA. The lung hydroxyproline was evaluated by alkaline solution. Results Compared to group R, the counts of macrophages in BALF in group P + R were reduced by 76% and 62%, and hydroxyproline levels were reduced by 21% and 24% at the 4th and 5th months, respectively. The plasma TGF-β decreased from the 3rd month to 5th month. Pirfenidone markedly ameliorated the severity of lung fibrosis at the 4 - 6th month after radiation. Conclusions Pirfenidone can prevent radiation-induced pulmonary fibrosis, the mechanism of which may be the reduced of inflammation and collagen deposition by decreasing macrophages and hydroxyproline.

Objective:To evaluate the inhibitory effect of intraperitoneal sustained-release chemotherapy with 5-FU on the growth of H22 ascitic tumor in mice.Methods: Mouse H22 ascitic tumor model was established by intraperitoneal injection of(0.2 ml) H22 ascitic cells(4?10~(6)cells) and the animals were subsequently divided into 4 groups randomly, namely,the saline control group(received saline),peritoneal chemotherapy group(received common 5-FU),sustained-release chemotherapy group(received sustained-release 5-FU),and negative control group(received control sustained-release agent).The survival times of the mice were recorded in all groups.The apoptosis rates of H22 ascitic cells were analyzed with flow cytometry 9 and 12 days after injection of H22 cells and the proliferation index was calculated.Electron microscope was used to observe H22 cells 12 days after peritoneal injection.Results: The average survival time of peritoneal chemotherapy group([13.7?1.7] d) was significantly shorter than that of sustained-release chemotherapy group([15.3?2.0]d)(P

Objective To study the effects of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on experimental allergic encephalomyelitis (EAE) and its immune-modulating mechanisms. Methods 1,25-(OH)2D3 were given to Lewis rats immunized with myelin basic protein (MBP) in complete Freud’s adjuvant (CFA) respectively on the same day of immunization (the prevented group) and the presenting day of symptoms (the treated group). Then clinical symptoms were scored until the 13th day after immunization, when rats were sacrificed. Mononuclear cells (MNC) in draining lymph nodes were collected for the following studies: (1)Total number of MNC in the lymph nodes were counted. (2) Numbers of CD~+4CD~+ 25 cells and CD~+ 86 cells were determinated by flow cytometry. (3) The concentrations of interferon(IFN)-? and interleukin(IL)-4 in cell culture supernatant were measured by enzyme-linked immuno sorbent assay (ELISA). Results 1,25-(OH)2D3 could delay the peak EAE severity in the prevented group while it could relieve the progression of EAE and lower the peak score in the treated group (3.3?0.6 vs 4.0?0.3, P

Objective To explore the clinical significance of tumor pathological length in evaluation of prognosis of esophageal squamous cell carcinoma.Methods 421 patients with esophageal squamous cell carcinoma were selected as study subjects.Each patient's data of gender,age,pathological examination(tumor pathological length),survival time,cancer stage,operation mode were collected.The patients were divided into two groups:≤4 cm group(189 cases) and >4 cm group(232 cases), and the constituent ratios of gender,age,or operation mode and the survival rate between the two groups were analyzed.Results There were no significant differences in gender composition,age structure and operation mode between the two groups(x2=2.03,2.41,0.06,all P>0.05);There was positive correlation between tumor pathological length and TNM stage(CMH=68.07,P<0.01).The 25%,50% and 75% quantiles of survival period in the group whose tumor length was less than or equal to 4 cm were 28,40 and 58 months,respectively,which in the group whose tumor length was more than 4 cm were 19,31 and 61 months,and there was significantly difference between the two groups(x2=44.88,P<0.01), the group whose tumor length was less than or equal to 4 cm had higher survival rate than the group whose tumor length was more than 4 cm.In the further stratification analysis by TNM stage,the group whose tumor length was less than or equal to 4 cm had higher survival rate than the group whose tumor length was more than 4 cm in theⅠstage patients(x2=5.61,P<0.05);the group whose tumor length was less than or equal to 4 cm had higher survival rate than the group whose tumor length was more than 4 cm in theⅡstage patients(x2=4.62,P<0.05);the group whose tumor length was less than or equal to 4 cm had higher survival rate than the group whose tumor length was more than 4 cm in the Ⅲ stage patients(x2=4.88,P<0.05).Conclusion Tumor pathological length is an effective prognostic factor for esophageal squamous cell carcinoma,and the critical value can be determined as 4 cm.

Objective To evaluate the radiosensitization effect of gemcitabine in human cholangiocarcinoma cells(QBC939)in vitro. Methods The IC10,IC50 and IC90 of gemcitabine in QBC939 cells were determined by clonogenic assay,which were adopted in the following experiments.The cells were divided into five groups:control group,chemotherapy group,radiation group,radiation after chemotherapy(C+R)group and radiation before chemotherapy(R+C)group.The radiation dose ranged from 1 Gy to 10 Gy.The cell survival Curves were fit using two-component equation and the sensitive enhancement ratio(SER)was calculated.Results The IC10,IC50 and IC90 of gemcitabine in QBC939 cells were 0.1 nmol/L(low concentration),11.0 nmol/L(moderate concentration)and 21.5 nmol/L(high concertration),resDectively.Gemcitabine in low concentration radiosensitized the cells at low irradiation dose(≤2 Gy)in R +C group(SERDq=1.52).Gemcitabine in moderate concentration had radiosensitization effect at all irradiation doses in C+R group(SERD0=1.27,SERDq=116.93),and at low irradiation dose(≤2 Gy)in R+C group.Gemcitabine in high concentration had radiosensitization effect at all irradiation doses in both R+C and C+R groups,which was most markedly at low irradiation dose(≤2 Gy)in R+C group(SERDq =323.30). Conclusions Gemeitabine has radiosensitization effect in QBC939 cells,which is based on the optimal scheduling and concentration.