Objective: To evaluate the quality of Radix bupleuri from different producing areas and make a comparison by using grey correlation analysis(GRA)and principal components analysis(PCA).Methods: Fifteen samples of Radix bupleuri were collected from different producing areas.The percentage of total ash, acid-insoluble ash, extract, saikosaponin a and saikosaponin d of Radix bupleuri from different habitats were determined.The grey correlation quality evaluation model was built for Radix bupleuri from different producing areas and the results were compared with those of PCA.Results: According to the grey correlation analysis on components in the different batches of Radix bupleuri , the samples from Shaanxi province were the most highly correlated with the optimal reference sequence (0.79), which suggested the best quality.Radix bupleuri Fructus from Hebei province was the most poorly correlated with the optimal reference sequence (0.23), which showed the worst quality.The results were basically identical with those of PCA, indicating good repeatability and stability.Conclusion: Grey incidence degree method and the established Radix bupleuri quality evaluation models can be used to evaluate the quality of Radix bupleuri .

BACKGROUND: Disturbance of mitochondrial fission and fusion (termed mitochondrial dynamics) is one of the leading causes of ischemia/reperfusion (I/R)-induced myocardial injury. Previous studies showed that mitochondrial aldehyde dehydrogenase 2 (ALDH2) conferred cardioprotective effect against myocardial I/R injury and suppressed I/R-induced excessive mitophagy in cardiomyocytes. However, whether ALDH2 participates in the regulation of mitochondrial dynamics during myocardial I/R injury remains unknown. METHODS: In the present study, we investigated the effect of ALDH2 on mitochondrial dynamics and the underlying mechanisms using the H9c2 cells exposed to hypoxia/reoxygenation (H/R) as an in vitro model of myocardial I/R injury. RESULTS: Cardiomyocyte apoptosis was significantly increased after oxygen-glucose deprivation and reoxygenation (OGD/R), and ALDH2 activation largely decreased the cardiomyocyte apoptosis. Additionally, we found that both ALDH2 activation and overexpression significantly inhibited the increased mitochondrial fission after OGD/R. Furthermore, we found that ALDH2 dominantly suppressed dynamin-related protein 1 (Drp1) phosphorylation (Ser616) and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation (Thr172) but not interfered with the expression levels of mitochondrial shaping proteins. CONCLUSIONS: We demonstrate the protective effect of ALDH2 against cardiomyocyte H/R injury with a novel mechanism on mitochondrial fission/fusion.

The insufficient fund is the"bottleneck"factor restricting the development of the general practitioner education and training in China.The article attempts to investigate how to found a scientific and effective mechanism so as to provide the thinking for transforming the current status.

Objective To evaluate the safety and efficacy of basic immunization with different sequential immunization programs of oral live attenuated poliomyelitis vaccine(OPV)and inactivated poliomyelitis vaccine(IPV). Methods Infants above two months of age residing in Pudong New Area,Shanghai,were selected for the study,and the basic immunization program of OPV full course(O-O-O group),sequential IPV and OPV(I-I-O group),and IPV full course(I-I-I group)were administered at 2,3 and 4 months of age,respectively. Relevant adverse reactions were proactively monitored by the parents of the participants via completing their own self-observation form after immunization. Before and after immunization,blood samples were collected from the upper arm vein,and the serum was separated. The neutralizing antibody levels of poliovirus in serum were measured by micro neutralization test,and the antibody positive rate and geometric mean titer(GMT)were calculated. Logistic multivariate regression analysis was used to research the effects of group,gender,household registration,birth mass and positive rate of typeⅠ/Ⅲ antibody before immunizationon the positive rate of type Ⅰ/Ⅲ antibody after basic immunization. Results There were one case of lethargy and one case of crying in O-O-O group, one case of lethargy in I-I-O group,and one case of crying in I-I-I group. The GMTs of type I neutralizing antibody in the pre-immunization O-O-O group,I-I-O group,and I-I-I group were 41. 39,8. 21,and 12. 56,and those of type Ⅲ neutralizing antibody were 7. 57,4. 02 and 8. 08,respectively. There was no significant difference in GMT between groups for typeⅠandⅢneutralizing antibodies before immunization(F = 2. 815 and 0. 608,P = 0. 061 and 0. 545,respectively). The positive rates of type Ⅰ antibody before immunization were 24. 04%,34. 07% and 41. 00%,respectively,with significant difference between groups(χ~2= 13. 459,P = 0. 001). The positive rates of type Ⅲ antibody were 13. 94%,9. 89% and 18. 00%,respectively,with no significant difference(χ~2= 5. 188,P = 0. 075). After immunization,the GMTs of type Ⅰ neutralizing antibody were 1 311. 84,1 812. 59 and 833. 24,and those of type Ⅲ neutralizing antibody were 911. 97,1 752. 68 and 419. 50,respectively,with significant differences between groups(F = 76. 848 and 202. 632,respectively,each P < 0. 001). After immunization,the positive conversion rates of type Ⅰantibody were 98. 56%,100% and 100%,and those of type Ⅲ antibody were 99. 04%,100% and 99. 44%,respectively,with no significant difference between the two groups(χ~2= 2. 973 and 1. 045,P = 0. 122 and 0. 789,respectively). In addition,group,gender,household registration,birth mass and positive rate of typeⅠ/Ⅲantibody before immunization had no effect on the positive conversion rate of typeⅠ/Ⅲantibody after basic immunization. Conclusion All three sequential immunization programs achieved good safety and high antibody positive conversion rates after basic immunization.

Objective To investigate the clinical effect of biofeedback electrical stimulation in the treatment of female stress urinary incontinence(SUI) and discuss the clinical significance.Methods Daily therapy for 40 min through biofeedback electrical stimulation along with pelvic muscle exercises was performed in 36 cases of female SUI for 20 days as a course of treatment.The curative effect was evaluated as regular intervals.Results In the 36 cases,26 caces were cured,5 caces were improved and 5 caees were invalid.The total effective rate was 86.1％.In the follow-up visit,there was 1 case of relapse(2.8％ ) but with moderate symptoms.Conclusion Biofeedback electrical stimulation combined with pelvic muscle exercises,as the therapy for mild and moderate female SUI,was a safe,reliable,feasible,convenient and effective clinical method with high curative ratio.

The recent research of opioid receptors has achieved great progress, especially in the field of the protection of cardiac muscles in ischemic preconditioning, which makes people pay more attention to the relation ship between opioid receptors and cardiovascular system. This article summarizes the studies of opioid receptors, the measures they take to modulate cardiovascular system and the way they participate in ischemic proconditioning both at home and abroad.

One case with dedifferentiated liposarcoma in proximal tibia accompanied by angiosarcoma was analyzed. The dedifferentiated liposarcoma was further analyzed by imaging, clinical and pathological features. One 51-year-old male patient was suspected to bone tumor due to space occupying lesion for 6 days in left proximal tibia and admitted in Changhai Hospital of Second Military Medical University of Chinese PLA in May 2007. The clinical features, including onset features, physical examination, blood routine, imaging alterations, pathological characteristics, operation approach, and follow-up period were retrospectively analyzed. The tumor was treated by curettage and inactivation, iliac grafting, bone cement filling, tibial plateau T type plate and screw internal fixation and tumor tissue biopsy. However, tumor relapsed 40 days postoperatively, and the patient was treated by amputation surgery. Two weeks after surgery, the patient developed emptysis and died due to multiple organ failure. Dedifferentiated liposarcoma accompanied by angiosarcoma displays no noticeable characteristics in imaging, clinical manifestation, and laboratory examination, which make it difficult to diagnose it. This tumor is highly malignant, and has short disease course, and poor prognosis.

The ischemia-modified albumin(IMA) forms after the human serum albumin experiences ischmia/reperfusion,which is a novel marker for myocardial ischemia.IMA exhibits a reduced capacity to bind to certain transition metals,such as cobalt.It can be measured by the Albumin Cobalt Binding test in serum.Several clinical researches suggested that IMA is an ideal biomarker for myocardial ischemia and an important assay for diagnosis of acute coronary syndromes.

Objective To study a new method for extracting agarose. Methods In 1.5% agar solution, adding the cation resin exchanged by NH~+_ 4 to take off metal ions, then using the OH~ - basic resin to absorb agaropectin. Results and Conclusion The extracting rate was 57%, gel strength reached 560 g/ cm~ 2 , the agarose has good electricity function by measuring the gentian violet electrophoresis and the DNA gel electrophoresis, so it is suitable for bioche- mistry and molecular biology electrophoresis. It is a kind of simple and low cost method for extracting agarose which better than the EDTA- 2Na method and DEAE cellulose method.

Nurse workstation, a typical client program of "No.1 Military" project, is trailed and speculated with Oracle tools such as TOAD, and then the general startup flow mode for "No.1 Military" project can be determined, which is instructive for its maintenance and programming.