Consumer Product Safety ; Drinking Water ; standards ; Equipment Safety ; Food Safety ; Public Health ; instrumentation ; methods ; Safety Management ; Water Supply

Environmental Monitoring ; methods ; Environmental Pollutants ; analysis ; Microchip Analytical Procedures

Objective To evaluate the hydrogen proton magnetic resonance spectroscopy(1H-MRS) in the diagnosis of brain diseases.Methods 1.5 Tesla MR scanner of GE Company was used,and single voxel technique was applied in 45 healthy subjects as control group and 115 patients presented with different brain lesions.The 1H-MRS metabolites waveform were evaluated,and the results were analyzed.Results The values of NAA,Cho,Cr and Lac,Lip and NAA/Cho as well as Cho/Cr from the patients showed a statistical difference to the control group,with P not more than 0.05.Conclusion The 1H-MRS results of lesions in the brain disease show statistical differences,and when combined with conventional MRI can contribute to a confident diagnosis.

The prevalence of asthma in pregnant women appears to be increasing.Pregnant women with uncontrolled asthma may have an increased risk of adverse perinatal outcomes.Optimal management of asthma during pregnancy includes avoiding or controlling asthma triggers,individualized pharmacologic therapy,objective monitoring of lung function,and patient education.Inhaled corticosteroids are the preferred treatment for the management of all levels of persistent asthma during pregnancy.It is safer for pregnant women with asthma to be treated with asthma medications than to have asthma symptoms and exacerbations.The goal of asthma management during pregnancy is to optimize maternal and fetal health.

MicroRNA,known as micro-RNA or miRNA,is a class of small non-coding RNA whose ma-ture products is ～22 nucleotides long.It negatively regulates gene expression at the post-transcriptional lev-el.More and more studies confirm the important role of miRNA in carcinogenesis and tumor development by reg-ulating cell apoptosis,and the research focuses on tumor-associated miRNA in tumor cell apoptosis may bring widest perspective on treatment and diagnosis of tumor in the future.

The treatments of infected bone defects are still a major clinical problem.In recent years,local delivery systems applied as anti-infection and bone repair material become a hot research topic.In this review,we discuss various influence factors such as osteoinductive growth factors,antibiotics,cartier material,extended release methods.We also introduce the recent progress in the development of local delivery system for the treatment of infected bone defects.

Objective:To prepare amphotericin B(AmB)-polybutylcyanoacrylate(PBCA) nanoparticle and evaluate its characteristics,so as to screen for the optimal preparation technique.Methods: AmB was loaded to blank PBCA nanoparticles by incubation to prepare AmB-PBCA nanoparticles.The surface of the nanoparticles was modified with polysorbate 80.The AmB concentration of AmB-PBCA-NP solution was calculated with the standard curve of AmB at D_(405),and the stability of solution was assessed.The diameter distribution of the particles was determined with Coulter Laser granulometer.The colloid solution of AmB-PBCA-NP was centrifuged and the supernatant was harvested to determine the concentration of AmB.The encapsulation rate,drug loading rate,and in vitro drug release were measured.The optimized preparation recipe was screened using diameter,drug loading rate,and encapsulation rate of the particles as indices.Results: The prepared AmB-PBCA nanoparticle had a regular spherical or elliptic surface,with a mean diameter of(69.01?28.56) nm.The curve of standard AmB was linear within the range of 1.12-5.60 ?g/ml: D_(405)=0.163 4c+0.006 6(r=0.999 3).The average recovery of AmB-PBCA-NP was(99.93%,) showing the solution of AmB-PBCA-NP was stable within 12 h.The nanoparticles showed a sustained drug release in vitro within 24 h.The optimized recipe was: DextranT-70 stabilizer without sodium deoxycholate,with a mean encapsulation rate of 56.10% and a drug loading rate of 82%.Conclusion: The current method for preparing AmB-PBCA nanoparticles is simple and can meet the requirements of pharmaceutics.

In recent years,more and more experts and scholars study enhancer of zeste homolog 2 (EZH2) and the relationship of the overexpression of EZH2 gene with the occurrence,development,metastasis,and prognosis of tumor,to explore the early diagnosis of cancer,monitoring metastasis and judging the prognosis to provide the new ideas and the methods,to provide the reference for the basic and clinical research.

Deep vein thrombosis is one of venous thromboembolism clinical manifestations,but also a serious,potentially dangerous disease.Thrombosis can occur in various parts of the body veins,and deep venous thrombosis is the most common.Even though most patients with DVT have no clinical symptoms or with mild symptoms,but failure to timely appropriate treatment is easy to progress to post thrombotic syndrome,shares bruises,stock white swollen,which can occur in severe pulmonary embolism and cause death.Although clinically in a variety of measures to prevent DVT formation,including physical prevention and drug prevention,but the incidence of deep vein thrombosis remains high,9-36％ after total hip arthroplasty,and 52％ after total knee arthroplasty.DVT is a disease combined with genetic and non-genetic factors,60％ DVT patients have hereditary risk factors.Currently genetic factors on the formation of DVT study find that clotting factor Ⅺ gene polymorphisms is associated with DVT.Coagulation factor Ⅺ (F Ⅺ) is a serine protease synthesized by the liver and plays an important role in the coagulation cascade process such as maintain the amplification process and the intrinsic pathway.Research on correlation between single nucleotide polymorphisms (SNPs) of F Ⅺ locus rs2289252 and rs2036914 and deep vein thrombosis has become popular.This paper reviews the discovery of coagulation F Ⅺ single nucleotide polymorphism associated with DVT,the degree of association between coagulation F Ⅺ single nucleotide polymorphism and the formation of DVT,risk allele on the clotting F Ⅺ single nucleotide polymorphism,clinical trials of coagulation F Ⅺ and the formation of DVT,coagulation F Ⅺ single nucleotide polymorphisms interact with other genetic polymorphisms,etc.Although the single nucleotide polymorphism in DVT formation mechanism is still vague,we believe that further study of single nucleotide polymorphisms associated with DVT will provide new approaches to prevention and treatment of DVT.

The enzymological characterizations of site-mutagenized rat recombinant DNA polymerase?, RQ182 and RQ183 were studied The phosphocellulose column chromatographies showed that the mutant and the wild DNA polymerases were all eluted by about 0.5 mol/ L KCI, but the denatured DNA-cellulose chromatographies showed that although the wild enzyme was eluted by 0.35 mol/L KCI, RQ182 and RQ183 were eluted by 0.55 and 0.45 mol/L KCI, respectively, indicating that the binding abilities to DNA of the mutant enzymes were increased. Km values for the substrate (dTTP)of the wild enzyme, RQ182 and RQ183 were determined as 38.5, 34.5 and 111.1 ?mol/L, respectively,and the Km values for the primer (oligo(dT)) were 1.28, 1.96 and 6.58 ?g/ml, respectively. The results showed that the affinities of RQ183 to the substrate and the primer were decreased dramatically. It is suggested that Arg182 and Arg183 were involved in the active site function of DNA polymerase ?, in binding to DNA template, in recognizing of primer, and in binding to and catalyzing of substrates of the enzyme.