One hundred mandibles of adult Chinese male unearthed in Shanghai without missing teeth, alveolar bone resorption or/and malposition of teeth were studied. Our data showed that the location of the mental foramen below the apex of the lower second premolar was the most common. Our results were compared with those of other investigators. The Clinical significance of iden.tifying the anatomical location of the mental foramen in dental practice is discussed

We tried to clarify the role of oxygen radicals [released from granulocytes stimulated by phorbol myristate acetate (PMA) in rat acute lung injury. It was found that DNA strand-break damage (DSBD) in peripheral white blood cells was significantly increased 40 min after injection of PMA. DSBD in lung tissue of rats treated with PMA was also markedly increased. The PMA-treated rats showed significantly higher lipid-peroxide (LPO) level in plasma and lung hemogenate than the controls. These results suggest that determination of DSBD, a simple and sensitive indicator for oxygen radical damaging, might be useful in the diagnosis of adult respiratory distress syndrome, when it is used together with measurement of plasma LPO.

BACKGROUND: Mental health is the most important internal resource that individual can depend on and also an important factor which affects the existence and development of individual. But there are few studies on the level and characteristics of mental health of female prisoners during serving a sentence.OBJECTIVE: To investigate the level and characteristics of mental health of the female prisoners in Shandong Province.DESIGN: Retrospective, cross sectional,multi-variable and sampling survey based on female prisoners.SETTING: Department of Technology in Shandong Police College.PARTICIPANTS: The investigation was completed in the Shandong Police College frotm January 2002 to March 2002.Totally 393 female prisoners from the Shandong Female Prison were selected with the grouping-sampling method to give the questionnaire and attend the investigation voluntarily,and the persons with definite mental disease were excluded.METHODS: The mental health items of female prisoners,including 9 factors such as somatization, obsessive-compulsion,sensitivity of human relations, depression, anxiety, hostility, phobia anxiety, paranoid ideation and psychosis,were explored with Symptom Checklist 90(SCL-90), and the judging standard of psychologic obstacle was whether the total scores of positive symptoms were 2 standard deviations more than those of the norm or not.And combined with the archives of the female prisoners and the questionnaire of self-editing item,relative data were collected. The uniform instructional words and the methods of filling in were explained to the female prisoners firstly,and then the answers of the questionnaire were written collectively.MAIN OUTCOME MEASURES: The mental health level and the psychological characteristics of female prisoners.RESULTS: 393 questionnaires were provided.There were 15 questionnaires excluded because more than 5 questions were missed in the paper or responsion consistency deviation appeared obviously after callback.378questionnaires were valid ,and the effective rate was 96.2%. ①The item numbers of positive reaction,total average score,toatal average score of positive symptoms of SCL of the prisoners were higher respectively than those of the norm of general group and reached the most significant level.The psychological problems of different degrees were widespread among them.②There were 372 female prisoners who had positive symptoms, and the detection rate was 98.41% ,which was more than that of positive symptoms in prisoners (40.6%).The significance of difference existed(x2=503.59,P ＜ 0.001).There were 84 persons who had psychologic obstacle in the female prisoners,and the detection rate was 22.2%,which was higher than that of in the general group (10.54%).The significance of difference existed (x2=54.128,P ＜ 0.001). ③The scores of 9 factors of SCL were all higher in the female prisoners than in the adult norm of the country,and reached the most significant degree. It showed that the obvious abnormality existed in the somatization, obsessive-compulsion, sensitivity of human relations, depression, anxiety, hostility, phobia anxiety,paranoid ideation and psychosis of the tested persons, and the ordering of them was sensitivity of human relations ＞hostility ＞obsessive obsessivecompulsion ＞depression ＞somatization ＞anxiety ＞phobia anxiety/paranoid ideation＞psychosis. Among the 9 factors,the average scores of obsessivecompulsion, sensitivity of human relations, depression,and hostility were greater than 2,and the symptomatic reaction of them was already above moderate. ④The detection rates of psychological problem or psychologic obstacle of the factors such as paranoid ideation, hostility, somatization,sensitivity of human relations and anxiety,etc.were higher,which indicated that these were the main psychological deviation contents.CONCLUNSION: Compared with the general group,the total level of mental health of female prisoners is lower, and the detection rate of psychologic obstacle and the degree of symptomatic reaction are obviously higher,and the psychological characteristics such as phobia anxiety, obsessive-compulsion,sensitive-suspiciousness, depression and hostility,etc are outstanding.

BACKGROUND: Every year there are case reports about freshmen who took extreme actions because of maladaptation. Freshmen's mental health is related not only to their adaptability to university life but also to the stability and harmony of university campus.OBJECTIVE: To investigate the level of freshmen's mental health in early university life and screen out the students with severe psychological problems.DESIGN: A cross-sectional study.SETTING: Department of Education, Shaoxing University of Zhejiang Province.PARTICIPANTS: A total of 4 417 freshmen of year 2003 from Shaoxing University were selected as observational subjects during November 2003.METHODS: The status of mental health was surveyed with symptom checklist-90 (SCL-90), self-rated anxiety scale (SAS), and self-rated depression scale (SDS). SCL-90 consisted of 9 dimensions: somatization, obsessive-compulsive, interpersonal sensitivity, depression, anxiety, hostility,phobic anxiety, paranoid ideation and psychoticism, and 5 evaluation scales: 1 as none, 2 as mild, 3 as moderate, 4 as severe and 5 as rather severe. The higher the score, the lower the level of psychological health was. They were evaluated as whether they had problem of psychological health or not. SAS contained 4 grades that recorded mainly the frequency of the appearing symptoms defined by the evaluation entry. The standard score took 50 points as the critical point. The higher the score, the more severe anxiety status was. The main statistical index of SDS was the exponent of depression severity that ranged from 0.25 to 1.00. Those with less than 0.05 had no depression, 0.50-0.59 mild depression, 0.60-0.69moderate depression, and above 0.70 severe depression. The group test was conducted among the freshmen 3 months after they entered the university.MAIN OUTCOME MEASURES: The scores of SCL-90, SAS and SDS.RESULTS: A total of 4 417 questionnaires were sent out, and 4 387questionnaires were collected with the effective rate of 99.32%. ① The scores on obsessive-compulsive, phobic anxiety and psychoticism ih the freshmen were higher than those of the norms (t=2.88-4.82, P ＜ 0.01). Among them, 333 freshmen with common psychological problems were detected, which constituted 7.59%. Another 49 students with severe psychological problems were screened out, which made up 1.12%. ② There were significant sex differences of scores in SCL-90 factors between boys and girls (total average t=5.56, P ＜ 0.01). ③ There was a significant correlation of depression and anxiety of SCL-90 scores with the standard scores of SDS and SAS, respectively, and the coefficient of product-moment correlation was 0.532 and 0.361, respectively.CONCLUSION: ① The freshmen did not have good psychological health.② The girls had more psychological problems than the boys. ③ The scores on depression and anxiety of SCL-90 were mutual proof with the standard scores of SDS and SAS, respectively.

Objective:To study the effect of adriamycin(ADR) as therapeutic agent inducing rabbit posterior capsular lens epithelial cell apoptosis and to assess the relationships between P53,Bcl-2 after lens extracapsular extraction.Methods:Twelve rabbit eyes were injected with 0.2mg ADR(0.4mg?L -1 ) during lens extracapsular extraction performed respectively in the third week and the eighth week after the operation.With TdT-mediated dUTP nick end labeling(TUNEL)and immunohistochemical(SABC) staining.The protein expression cell rate of P53 and Bcl-2 were detected.Results:Positive pericyte muclei labeled by TUNEL increased more significantly than those in the control guoup from the third week to the eighth week after the operation.P53 protein expression was significantly higher(P

Objective:To investigate the relationship among inflammatory reaction and cytokine levels,nitric oxide(NO) content in aqueous humor after alkali burn.Methods:12 adult New Zealand rabbits were divided into two groups(6 rabbits each):normal control group and alkali burn experimental group.Aqueous humor samples were aspirated on the alkali burn 1,3,7,14 and 30 days,and the total number of white blood cells in the aqueous humor and their subset distribution as well as tumor necrosis factor-? (TNF-?) and NO~-_2/NO~-_3 were counted.The data were analyzed using analysis of variance of SPSS software.Results:There was a higher number of inflammatory cells and a higher level of TNF-? and NO~-_2/NO~-_3 in alkali burn eyes than that in the normal control eyes.The number of mononuclear phagocyte and the levels of TNF-?,the content of NO~-_2/NO~-_3 in aqueous humor were the highest in alkali burn group in 7～14 days.The change of TNF-?,NO~-_2/NO~-_3 and inflammatory reaction were basically similar,that is ,the more serious the reaction,the higher the levels of the content.Conclusion:The intraocular inflammation after alkali burn is closely related to the change in cytokine levels and NO content in aqueous humor.

Objective:To establish mice model with learning and memory deficit induced by cerebral ischemia/reperfusion.Methods:The mice model induced by cerebral ischemia/reperfusion were made by bilateral occlusion of both common carotid arteries and ischemic hypotension/reperfusion.Locomotive activity,step-down test and water maze were used to reflect behavior changes,as well as cell morphologic changes were evaluated,respectively.And total anti-oxidant capacity and activities of SOD(superoxide dismutase)and MDA(malondialdehyde)were also measured.Results:The neuronal damage in model mice was significant,compared with sham group.And locomotive activity of model mice markedly decreased.The escape latency and time of electric shock of model mice significantly increased in step-down test;and 24 hours later the number of errors significantly increased,step-down latency and time of remaining on platform markedly decreased.In maze test,the spatial learning and memory ability of model mice significantly decreased with characteristics of increase in the number of errors and latency at D3,D4 and D5,and the total anti-oxidant capacity and activities of SOD were decreased and MDA level increased in model group compared with sham group,respectively.Conclusion:This model of mice cerebral ischemia/reperfusion is useful for studying learning and memory deficit.

AIM: To determine the content of ginsenoside Rg_1、Re and Rb_1 in Shenfu Injection (Radix et Rhizoma Ginseng Rubra,etc.) by HPLC-ELSD. METHODS: Separation was achieved on a Waters ODS column((4.6) mm?150 mm,5 ?m).Elution was carried out using a gradient of acetonitrile and water.Drift tube temperature:120 ℃,gas flow rate:1.8 mL/min. RESULTS: The calibration curves were linear in the range of 0.322-(3.22) ?g for ginsenoside Rg_1,0.290-2.90 ?g for ginsenoside Re,0.508-5.08 ?g for ginsenoside Rb_1. CONCLUSION: The method is simple,accurate,effective and feasible.It can be used to control the quality of Shenfu Injection.

Objective:To determine the expression of aquaporin 8 and its localization in human WISH cells and to explore the molecular and cellular mechanisms for water absorption across the amniotic membranes.Methods:Human amnion-derive WISH cells were cultured.Western analysis was used to quantify AQP8 expression level.Reverse transcripton-polymerase chain reaction(RT-PCR)was used to quantify AQP8 mRNA expression levels.Immunofluorescence was used to determine localization of AQP8 in WISH cells.Results:AQP8 mRNA protein was found in human WISH cells.By western-blot,the AQP8 proteim was detected at 34ku in human WISH cells.AQP8 labeling was observed in intracellular vesicular structures throughout the cytosol and plasma membrane.Conclusion:The study demonstrates the expression of aquaporin 8 in human WISH cells.These results suggest that aquaporin 8 may be a channel that mediates amniotic fluid resorption by way of intramembranous pathway.

Objective To summarize analysis postpartum hemorrhage were treated by human recombinant coagulation factor Ⅶ, CARDS and other three amine butyl alcohol, psychological intervention treatment of clinical effects and the patient's quality of life.Methods Our hospital during July 2013 to December 2016, total 100 cases of postpartum hemorrhage patients treated with the control group of 50 patients with card other three amine butyl alcohol with psychological intervention therapy;observation group of 50 cases using human recombinant coagulation factor Ⅶ, CARDS and other three amine butyl alcohol, psychological intervention treatment, two groups of patients were reviewed treatment effect and postpartum quality of life.Results In the bleeding, bleeding time, postpartum anxiety score, depression score indicators, such as observation group is less than the control group, the difference to be markedly, with statistical significance (P<0.05);In the postpartum life quality score, the observation group is higher than the control group, the obvious differences, statistically significant (P<0.05);In terms of adverse reaction happening, observation group and control group difference is not obvious, not statistically significant .Conclusion Human recombinant coagulation factor Ⅶ, CARDS and other three amine butyl alcohol, psychological intervention treatment of postpartum hemorrhage, the clinical curative effect is distinct, can better improve the patients quality of life, improve patients' negative mood, is worthy of popularization and application.