Objective:Toconstruct the shRNA(Short Hairpin RNA)eukaryotic expression vector targetingat IER3IP1 gene and observe its silencing effect on IER3IP1 gene.Methods:Two pairs of DNA fragments targeting at IER3IP1 gene and one pair of DNA fragment of negative control were synthesized and cloned into the Eukaryotic Expression Vector pGenesil-1.These vectors were separately transfected into the L02 cells.Inhibitory effect at the time of 24,48,72 h after transfection were detected by semiquantitative RT-PCR.Results:These shRNA eukaryotic expression vectors were successfully constructed.One of them could significantly and specially inhibite the expression of IER3IP1 gene and the inhibition ratios were separately 64%,79%and 69%at the time of 24,48 and 72 h,compared with empty vector group.Conclusion:The combinated eukaryotic expression vector could inhibit the expression of IER3IP1 gene,which forms the basis for investigating IER3IP1 gene's function.

ObjectiveTo observe the clinical efficacy of warming-unblocking Yin Heel Vessel plus rehabilitation training in treating post-stroke strephenopodia, for seeking the optimal treatment protocol.MethodNinety eligible subjects withpost-stroke strephenopodia were randomized into an acupuncture group, a rehabilitation group, and a united group, to respectively receive warming-unblocking Yin Heel Vessel, rehabilitation treatment, and warming-unblocking Yin Heel Vessel plus rehabilitation training. ResultTwo treatment courses later, there was no significant difference in comparing the strephenopodia score between the acupuncture group and rehabilitation group (P>0.05); the strephenopodia score in the united group was significantly different from that in the acupuncture group and rehabilitation group (P<0.05), and the improvement in strephenopodia in the united group was superior to that in both acupuncture group and rehabilitation group.ConclusionWarming-unblocking Yin Heel Vessel plus rehabilitation can produce a content efficacy in treating post-stroke strephenopodia.

[Abstract ] Tumor, with its high morbidity and mortality, has been gained increasingly high attention from the world .Current-ly, the main treatment for solid tumor is still surgical resection , during which surgery , anesthesia and other factors may have such side effects as releasing tumor cells into blood , lymphatic , bone marrow even organs and then resulting in the formation of micrometastatic lesion, increasing the risk of tumor recurrence and metastasis and finally affecting the postoperative survival rate .Recently, studies have shown that most anesthetic agents can influence the function of immune system and the activity of tumor cells , and then impact the tumor micrometastasis .This paper will summarize the research progresses in the impacts of anesthetic agents on tumor micrometastasis during perioperative period .

Objective To study and explore Glucose-reducing effect of induced human bone mes-enchymal stem cells on diabetic mice.Methods The diabetic model of rats was duplicated by injection of STZ intraperitoneally.The induced cells were implanted into diabetic mice.Blood glucose levels were moni-tored every 3 days after implantation for 14 days.Results The mice receiving the treated Cells began to decrease their blood glucose levels after 3days.But control Animals that did not receive induced cells exhib-ited persistent hyperglycemia.Conclusions Induced cells by hBMSCs can decrease blood glucose levels on diabetic mice.

To observe the pathological changes of vascular aging in spontaneously hypertensive rats (SHRs), and the effects of extracts from Panax ginseng, Panax notoginseng (Burk.) and Ligusticum chuanxiong on vascular aging.

Objective To detect normal rat jejunum epithelial cells to learn about the distribution of peptide YY(PYY)receptor and measured two important parameters of PYY receptor so as to explore the target that PYY binds to and display its physiological function.Methods Receptor radioligand assay was conducted by using 125I-PYY to detect normal rat jejunum epithelial cells.We also measured receptor dissociation constant(Kd),the affinity or the ability that the receptor could bind ligand;maximum binding capacity(Bmax),the concentration of binding site;and Hill coefficient.Results There were PYY receptors in normal rat jejunum epithelial cells.Dissociation constant(Kd)of PYY receptor was(386.69?129.95)pmol/L.Maximum binding capacity(Bmax)was(303.21?116.85)fmol/mg protein.Hill coefficient was 1.PYY receptor had high affinity.Conclusion There are distributions of PYY receptors in normal rat jejunum epithelial cells.Kd means that PYY receptor has high affinity.Bmax means that the number of receptors is limited and decides the characters of saturability.We think that PYY directly inhibits secretion by binding to PYY receptor in jejunum epithelial cells when PYY inhibiting jejunum secretion.

Objective To mine the relation between leukemia and genes using Weka. Methods The papers on leuke-mia and genes were retrieved from PubMed, their subject headings and subheadings were extracted using BICOMB to generate co-occurrence matrix and term-paper matrix. The research hotspots were found by cluster analysis of the data on co-occurrence matrix using Weka and Cobweb. The literature was verified. Results The 42 high fre-quency words were clustered into 7 classes by Weka. No high frequency words of leukemia or genes were found in classes 1, 2, 4 and 5, indicating that their clustering efficiency was poor. The clustering efficiency of the other 3 classes was good. Conclusion Cluster analysis showed that leukemia is related with myc gene, ab1 gene, p53 gene, virus gene, immunoglobulin gene and mdm gene.

Objective To explore the correlation of urine albumin and creatinine ratio(UACR)and short -term prognosis in patients with acute ischemic stroke (AIS).Methods 112 patients with AIS were selected as the research subjects.The admission UACR and related laboratory examination indexes were detected,the US national institutes of health stroke scale (NIHSS)scores,and 28 d after admission of the modified Rankin scale (mRS) prognostic score were recorded.The NIHSS 5 or more points and NIHSS <5 points,different levels and UACR patient observation index between the different prognosis group were compared.Results NIHSS 5 or more group of patients on admission UACR level[(15.41 ±3.21 mg/g)]was higher than the NIHSS <5 patients (9.52 ±2.04)mg/g (t =12.214,P <0.001);UACR acuity 11.60mg/g group in NIHSS of 5 or more proportion of patients (87.5%),mRS score >2 points (82.1%),the proportion of the recent death patients (23.2%)than the proportion of UACR <11.60mg/g,the proportion of the group (32.1%,25.0%,7.1%;the chi -square values 33.509,34.476,5.598,all P <0.050);MRS score >2 groups of patients UACR level[(16.10 ±3.29)mg/g]was higher than that in patients with mRS score 2 points or less (8.38 ±1.95)mg/g(t =20.132,P <0.001);Pearson correlation analysis showed that the standard of UACR and NIHSS score (r =0.412,P <0.05 ),high -sensitivity C -reactive protein (hs -CRP)(r =0.473,P <0.050),white blood cell count (WBC)(r =0.442,P <0.050),fasting plasma glucose (FPG)(r =0.375,P <0.050)were positively correlated.Multiariable Logistic regression analysis showed that UACR (OR =1.587,P <0.050)was a recent independent risk factors of poor prognosis in patients with AIS. Conclusion UACR the illness severity and recent prognosis of patients with AIS is closely related to high levels of UACR may be recent independent risk factors of poor prognosis in patients with AIS.

Objective: To assess the mental health status of poor medical students.Methods: 40 poor medical students and 40 ordinary medical students were studied controlly with symptom checdlist-90. Results: poor medical students than ordinary medical students had more scores in factors and all average. Conclusion: the mental health status of poor medical students is not good.The work of mental health education and consultation should be accessed according to their mental status for them.

Embodied as an abstract concept as well as concrete regulations and material environment on the campus,the campus culture is the organizational culture among universities,which plays a significant role in enhancing students' quality of career starting.Similar to its social culture,the American campus culture is characterized by a strong personal heroism thus is known as a "cowboy culture".As the so-called "dragon culture",the Chinese culture is a more conservative one and thus lack of innovation-oriented career starting concepts among Chinese university students.Therefore,in order to effectively carry out entrepreneurship education in colleges and universities,the innovation-oriented campus culture should be constructed,students' entrepreneurship conception strengthened,thus students' sense of career starting and mental status be enhanced.